가감좌귀음(加減左歸飮)의 알레르기성 기관지천식(氣管支喘息) 반응(反應) 조절(調節) 효과(效果)

박은정,이해자,박종익,박영주,Park, Eun-Jung,Lee, Hai-Ja,Park, Jong-Ik,Park, Young-Joo 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objective : KagamJwagwiEum(KJE) has been used for the purpose of prevention and treatment of bronchial asthma and allergic asthma in Korea. To investigate the biological effect of KJE, the author examined cytotoxicity and inflammatory cytokines secretion with human leukemic mast cell line, HMC-1. Methods: HMC-1 was stimulated with phorbol 12-myristate 13-acetate(PMA) and calcium ionophore A23187. KJE by itself had no effect on viability of HMC-l. The effects of KJE on the secretion of tumor necrosis $factor-alpha(TNF-{\alpha})$, interleukin(IL)-6 and IL-8 from HMC-1 were evaluated with enzyme-linked immunosorbent assay. Results : KJE inhibited PMA plus A23187-induced $TNF-{\alpha}$ and IL-6 secretion. But KJE had no effect IL-8 secretion: KJE had immunoregulatory effects on cytokines, increased secretion of NO and $TNF-{\alpha}$ but did not effect IL-12 secretion when the cells were primed and trigged with $IFN-{\gamma}$ in the peritoneal macrophages of C57BL/6 mice. Conclusions : Taken together, these results suggest that KJE inhibit the production of inflammatory cytokines in HMC-1 cells and activate macrophages.

생약으로부터 세포분화유도물질의 검색 및 분리 및 분리 (I)

박은정,김진웅,Park, Eun-Jung,Kim, Jin-Woong 한국생약학회 1997 생약학회지 Vol.28 No.4

300 extracts derived from 100 plants were tested for their potential to induce HL-60 cell differentiation using NBT assay and NSE/SE staining methods. Morphological changes from suspended to adherent state of the cells were also observed by microscopic examination. In result, 55 extracts induced cell differentiation into monocyte/macrophage lineage in the NBT and the NSE assay.

당귀음자(當歸飮子) 가감방(加減方)의 처방별 (A, B) 아토피성 알레르기반응 조절 효과 비교 연구

박은정,이해자,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Han, Kyeung-Hoon 대한한방소아과학회 2005 대한한방소아과학회지 Vol.19 No.2

Objective: Danguieumja-gagambang (DGEJGB), a traditional Korean prescription, has been used as therapeutics for allergic diseases such as atopic dermatitis (AD). In this study, we compared with regulatory Effect of Atopic Allergic Reaction by Prescriptions A and by Prescriptions B. Methods : To evaluate and compare the atopic allergic effectiveness of two prescription (A, B) of DGEJGB, the author investigated a possible effect of DGEJGB on mast cell-mediated allergic reaction, cytokine secretion and mRNA expression in vivo and in vitro. Results : Mast cells are a potent source of mediators that regulate the inflammatory response in allergic reaction. In mice orally administered A, B of DGEJGB ( 0.1, 0.1 and 1.0 g/kg) for 1 h, compound 48/80-induced ear swelling was significantly reduced. Significant reduced levels (P < 0.05). of tumor necrosis factor $(TNF)-{\alpha}$ was observed in the human mast cell line (HMC-1) with DGEJGB (A). IL-6 and IL-8 secretion were significantly inhibited by DGEJGB (A, B). In addition, $TNF-{\alpha}$ and IL-8 mRNA expression were reduced by DGEJGB (A) at the dose of 0.01 mg/ml without cell toxicity. Conclusions : These results suggest that DGEJGB (A) contributes to the treatment of atopic allergic reactions rather than DGEJGB (B), and that its action may be due to inhibition of cytokine secretion and mRNA expression HMC-1.

소아중풍(小兒中風) 환자(患者)에 대한 치험(治驗) 1례(例)

박은정,이해자,나원경,장성진,안재선,한경훈,Park, Eun-Jung,Lee, Hai-Ja,Na, Won-Kyeung,Jang, Sung-Jin,Ahn, Jai-Sun,Han, Kyeung-Hoon 대한한방소아과학회 2004 대한한방소아과학회지 Vol.18 No.2

Objectives : The purpose of this study is case report of stroke in childhood. The childhood cerebrovascular diseases are interesting than those seen in adulthood, because the etiology and prognosis of the disease in children are quite different from those of adults. Cerebrovascular diseases in children is more common than once recognized. However, relatively little attention has been paid to the epidemiology of childhood stroke. Methods : We experienced one case of ischemic stroke in childhood whose symptoms are similar to that of adults. There is no evidence of cerebrovascular malformation at MRI, MRA film. The cause of stroke is undetermined and just we can see the little infarction lesion on Lt. pons. We treated the patient with herb medicine, acupuncture-Tx, negative-Tx, moxa-Tx and physical treatment. Results : After the treatment of oriental medicine, the patient recovered from the stroke symptoms. Conclusion : we thought that the more follow-up is needed. So we repoert one case of cerebral infarction in children that recovered from stroke symptoms after the treatment of oriental medicine.

당뇨병쥐 뇌에서 Gangliosides 분석

박은정,전길자,Park, Eun-Jeong,Jhon, Gil-Ja 생화학분자생물학회 1993 한국생화학회지 Vol.26 No.6

본 연구에서는 alloxan으로 유발시킨 당뇨병 쥐의 뇌에서 gangliosides 조성 변화를 조사하였다. 뇌 조직은 alloxan 주사후 3일째 희생시켜 얻었으며, gangliosides는 Folch-Suzuki 방법을 이용하여 추출하였다. Periodate-resorcinol 법으로 sialic acids를 정량한 결과, 당뇨병 쥐 뇌에서 sialic acid 양$(375{\mu}g)$이 정상$(783{\mu}g)$에 비해 반으로 감소하였다. 당뇨병 쥐 뇌에서 GT1b, GD1b, GD1a는 감소하였고 GM3와 band 1은 증가하였다. Sialidase처리로 band 1은 sialic acid가 없는 당지질임이 확인되었다. 2D-HPTLC로 당뇨병 쥐 뇌에서 GM1a와 GD1a 사이에 존재하는 마확인된 ganglioside가 증가함이 밝혀졌다. 미지의 ganglioside를 preparative TLC로 분리하여 ${\beta}-galactosidase$를 처리한 후 HPLC로 확인한 결과 Gal-GM1, Gal-Gal-GM1임이 확인되었다. The changes of ganglioside pattern from alloxan-induced diabetic rat brain, were investigated. Gangliosides were isolated by Folch-Suzuki method and analyzed by HPTLC. Sialic acid was determined by periodate-resorcinol method. The amount of gangliosides decreased 2 folds in diabetic brain. We found decreasing GT1b, GD1b, and GD1a, increasing GM3 and band 1, and no change of GM1a. In diabetic brain band 1 was identified as simple glycolipid with no sialic acid by the treatment of sialidase. The increase of unidentified ganglioside separated between GM1a and GD1a in diabetic brain found by 2-dimensional HPTLC. Unidentified ganglioside was collected by preparative HPTLC, treated with ${\beta}-galactosidase$ and analyzed by HPLC. As the result, this ganglioside revealed Gal-GM1 and Gal-Gal-GM1.

독사 교상후 발생한 전신적 합병증 - 혈액학적 합병증과 신경학적 합병증을 중심으로 -

박은정,윤상규,안정환,최상천,김기운,민영기,이국종,정호성,정윤석,Park, Eun-Jung,Yoon, Sang-Kyu,Ahn, Jung-Hwan,Choi, Sang-Cheon,Kim, Gi-Woon,Min, Young-Gi,Lee, Kuk-Jong,Jung, Ho-Sung,Jung, Yoon-Seok 대한임상독성학회 2009 대한임상독성학회지 Vol.7 No.2

Purpose: This study explored and evaluated the systemic complications resulting from the bite of Korean venomous snake, focussing on hematologic and neurologic features. Methods: Medical records (demographic data, clinical measurements including laboratory results, severity score, and amount of antidote administration, and hospitalization course) of consecutive patients who presented with snakebites to two university teaching hospital during a 10-year period were retrospectively reviewed. Subgroup analysis was conducted for evaluations of anti-acetylcholine esterase administration in complicated victims. Results: The 170 patients displayed occurrence rates of hematologic and neurologic complications of 12.9% and 20.6%, respectively. Among 22 patients with hematologic complications, isolated thrombocytopenia was evident in eight patients (36.4%), prothrombin time (PT) / activated partial thromboplastin time (aPTT) prolongation in 11 patients (50.0%), and both in three patients (13.6%). The mean time to recovery was $4.5{\pm}1.8$ days for isolated thrombocytopenia, and $5.1{\pm}1.8$ days for PT and aPTT prolongation. Hematologic complications could occur suddenly 1?4 days after hospitalization. Among 35 patients with neurologic complications, dizziness was evident in 16 patients (45.7%), and diplopia / blurred vision in 19 patients (54.3%). The mean time to recovery was $3.4{\pm}0.6$ days in patients receiving anti-acetylcholine esterase and $6.9{\pm}1.8$ days in those not receiving anti-acetylcholine esterase (p=0.00). Conclusion: Occurrence rates of hematologic and neurologic complications following venomous snake bite differed as compared to other studies conducted in Korea. Onset of hematologic complications can occur rapidly days after admittance. Anti-acetylcholine esterase administration may be effective in treating neurologic complications.

사물탕(四物湯) 가미방(加味方)이 흑색중(黑色腫) 세포고사(細胞枯死)에 미치는 효과(效果)

박은정,이해자,장성진,Park, Eun-Jung,Lee, Hai-Ja,Chang, Sung-Jin 대한한방소아과학회 2006 대한한방소아과학회지 Vol.20 No.1

Objective : In this study, the ability of Oriental medicine Samultanggamibang(SMTG) to induce apoptosis was investigated in B16F10 melanoma cells. Method : Tetrazolium-based colorimetric assay was performed for cytotoxicity test. Several new assays for the basis of biochemical events associated with apoptosis such as DNA fragmentation by a flow cytometry, caspase-3 activation and PARP cleavage by Western blotting should be carried out potentially useful for the basis of biochemical events associated with apoptosis such as a flow cytometry and caspase-3 activation. Results : (1) The number of B16F10 melanoma cells was less than 30 % after exposure to 1 mg/ml SMTG for 48 h. SMTG increased cytotoxicity of B16F10 melanoma cells in a dose- and time-dependent manner. (2) The percentage of apoptotic cells by flow cytometric analysis of the DNA-stained cells increased to 21 % at 24 h and 25 % at 48 h after treatment with 1 mg/ml SMTG. (3) SMTG-induced apoptosis was accompained by the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymerase. (4) SMTG induces the activation of caspase-3 and the specific proteolytic cleavage of poly-ADP-ribose polymearse and eventually leads to apoptosis through c-Jun NH2-terminal protein kinase (JNK)-dependent manner in B16F10 melanoma cells. Conclusion : SMTG had a strong cytotoxic effect of B16F10 melanoma cells.

랫드 심근세포유래 $H_9C_2$ 세포주에서의 망간화합물의 산화적스트레스 유도작용

박은정,박광식,Park, Eun-Jung,Park, Kwang-Sik 대한약학회 2008 약학회지 Vol.52 No.3

Manganese is a naturally occurring element which is widespread in the environment. Also, manganese is an essential trace element and plays a key role in important biological reactions catalyzed by enzymes. However, exposure to high levels of manganese can cause toxicity in neurone and inhalation system, also damage in various tissues. We investigated the toxicity induced by manganese compound ($MnCl_2$) in cultured rat cardiomyocytes. Treatment of manganese to cultured cardiomyocyte led to cell death, reactive oxygen species (ROS) increase, and cytosolic caspase-3 activation. The ROS increase was related with the decreased level of glutathione. Expressions of ROS related genes such as heme oxygenase-1, thioredoxin reductase, and NADH quinone oxidase were significantly induced in manganese treated cells. These results suggest that manganese induce oxidative stress and apoptosis in cardiomyocytes, and may be the one of risk factors to cause heart dysfunction in vivo.

학습경험을 바탕으로 학생들이 제시하는 고등학교 화학교과 내의 어려운 개념과 문지방개념 분석연구

박은정,Park, Eun Jung 대한화학회 2014 대한화학회지 Vol.58 No.1

본 연구는 과학학습의 어려움이나 낮은 흥미도의 한 원인으로 과학 교과학습에서의 중요한 통로 혹은 입구에 해당하는 문지방개념의 존재를 가정하고 특히 화학의 어떠한 개념들이 여기에 해당하는지를 알아보았다. 또한, 각 개념의 속성이 무엇이며 개념을 이해하고 "아~하"의 깨달음을 얻는 경험은 어떠했는지도 함께 알아보았다. 이를 위해, 화학 II를 학습한 239명의 고등학생이 연구에 참여하였고 설문에 대한 응답으로 화학 교과의 어려운 개념이 무엇인지, 문지방에 해당하는 개념이 무엇인지, 혹은 문지방개념을 이해한 경험이 화학 학습에 어떠한 영향을 주었는지를 설명하였다. 몰과 원자구조가 화학 교과의 문지방개념으로 제시되었고 구체적으로는 제시된 두 개념이 문지방개념이 되는 속성이 무엇인지를 집중적으로 분석하였다. 문지방을 극복하고 이해하는 것은 각자의 경험에 따르지만, 문지방개념을 분석하는 기준은 각각의 경험에 일정한 준거를 제시하여 서로 다른 경험들을 객관화 시킬뿐 아니라 개념의 과학적 의미와 본성을 잘 드러내어 준다. 특히, 교사가 제시하는 화학의 문지방개념을 조사한 사전연구와의 비교는 문지방개념의 통합적 속성이 학생들의 학습과 과학흥미도 증진에 중요함을 보여준다. Concerning the difficulty of learning science and reduced interest in science, the authors of this study searched for potential threshold concepts which are portals or gateways in the field of science (particularly chemistry). The nature of these concepts and how to overcome their troublesomeness were further questioned. For this study, 239 high school students completed chemistry II provided information about what difficult concepts and potential threshold concepts in high school chemistry are and how they affect learning chemistry. In particular, the mastery experience of the threshold concepts was explored in detail. Two, "mole and atomic structure" were selected as threshold concepts in chemistry. Not only as important but also as threshold, this study emphasized the importance of the two concepts in terms of features characterizing them as threshold concepts. In particular, the features objectify subjective experiences of students and provide information describing the scientific meaning and distinctive nature of threshold concepts in science. Along with the data from teachers, this study shows the integrative feature as key criteria for students to make meaningful understanding of the two threshold concepts.

Effects of HyangsosanGamibang on the $PPAR{\gamma}$ in the bronchial asthma mouse model

박은정,이해자,오지은,Park, Eun-Jung,Lee, Hai-Ja,Oh, Ji-Eun The Association of Korean Oriental Pediatrics 2007 대한한방소아과학회지 Vol.21 No.1

목적 : OVA에 유도된 천식 쥐 모델에 향소산가미방을 투여한 후 $PPAR{\gamma}$의 변화를 조사하여 향소산가미방의 천식 치료 기전을 알아보고자 하였다. 대상 및 방법 : 8주된 암컷 BALB/c 마우스에 첫 날과 14일 후에 20 ${\mu}g$의 OVA를 알루미니윰 하이드록사이드 1mg과 혼합한 후 총 200 ${\mu}g$를 복강 내로 주입하여 감작시켰다. 처음 감작시킨 날로부터 21,22,23일 후에 천식 모델에 사용하는 초음파 분무기를 이용하여 세 번째 감작시켜서 천식 마우스 모델을 만들었다. 천식 마우스 모델을 만드는 기간 중 OVA를 복강 내로 주입한 후 19일째에 24시간의 간격으로 향소산가미방을 7일 동안 경구 투여하여 향소산가미방의 효과를 조사하였다. 기관지폐포세척술은 마지막 감작 후 72시간 후에 실행하고 기관지폐포 세척액의 총 세포수를 측정하였다. $PPAR{\gamma}$의 발현은 천식 마우스 모델의 폐와 향소산가미방을 투여한 마우스 모델의 폐를 적출한 후 Western blotting 방법을 아용하여 측정하였다. 병리 조직학적 검사는 hematoxylin 2 and eosin-Y 염색을 이용하여 조사하였다. 결과 : 정상 군과 비교하여 OVA감작 천식 쥐 모델에서는 72시간 후에 총 세포 수가 증가하였다. 특히 OVA감작 천식 군에서 증가된 호산구의 수가 향소산가미방을 투여 한 쥐 군에서는 유의하게 감소하였다. OVA감작 천식 쥐 모델에서 72시간 후에 정상 군과 비교하여 핵 내에서의 $PPAR{\gamma}$단백질의 발현이 약간 증가하였다. 그러나 향소산가미방을 투여한 쥐 모델에서는 세포질과 핵 내에서 $PPAR{\gamma}$단백질의 발현이 유의하게 증가하였다. 조직학적 검사상 정상 군과 비교하여 OVA 감작된 천식 쥐 모델에서는 폐포, 세기관지, 기도내강 주변에 많은 염증 세포들이 있었다. 그러나 가미향소산 을 투여 한 후에는 염증 세포들이 유의하게 감소하였다. 결론 : 가미향소산은 $PPAR{\gamma}$작용제로서 역할을 하며, 천식에 대한 치료제 또는 예방제를 개발하는 데 후보 물질이 될 것으로 사료된다.