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태아 쥐와 임신 쥐의 간에서 Protein Carboxyl Methylation
전길자,최혜정,이준승 梨花女子大學校 韓國生活科學硏究院 1990 韓國生活科學硏究院 論叢 Vol.46 No.-
Protein carboxyl methyltransferase(EC 2.1.1.77) which has specificities for the protein containing abnormal aspartyl residues is investigated in lysosome, mitochondria, peroxisome in liver of fetus, pregnant rat and vacuole vesicles in beet root. The level of this enzyme in brain cytosol of normal rat was almost same as that in brain cytosol of pregnant rat. But in liver cytosol, PCM activity of fetal rat was increased 17-fold more than that of normal rat. Total enzyme activities in lysosome, mitochondria and peroxisome of fetal and pregnant rat were not changed. But the total methylatability of fetal rat were increased about 2-fold comparing to pregnant rat. Vacuole vesicles showed the enzyme activities and methylatabilities.
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쥐 간의 O6 - methylguanine - DNA Methyltransferase 에 관한 연구
전길자,박종옥,김상덕 ( Gil Ja Jun,Jong Ok Park,Sang Duk Kim ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.4
Partial hepatectomy produced a 4-5 fold increase in the O^6-mG-DNA methyltransferase activity of rat liver homogenate. to catalyze the removal of methyl group in O^6-methylguanine from DNA. This enzyme activity was maximal at 48 h after partial hepatectomy. When the 3H-methylated enzymes of the normal liver and partially hepatectomized liver were compared on DEAF-cellulose (Whatman DE-52) chromatogram, there was no difference in the elution pattern of enzymes but difference in the protein profile. Both the methylated enzymes were eluted at 0.17 M NaCl.
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Protein methylase 2 에 의한 Myelin 염기성 단백질의 Carboxyl - methylesterification
전길자,홍성열,이향우,김명희,Martin Tuck,김상덕,백운기 ( Gil Ja Jun,Sung Ryul Hong,Hyang Woo Lee,Myung Hee Kim,Martin Tuck,Sang Duk Kim,Woon Ki Paik ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2
Bovine brain myelin basic protein (MBP or AI basic protein) was found to serve as an excellent in vitro substrate for S-adenosylmethionine:protein-carboxyl 0-methyltransferase (protein methylase II; EC 2.1.1.24) with a K_m value of 4.0×10^(-5)M. Peptide mapping of pepsin-digested [methyl-^(14)C]-MBP methylated by purified protein methylase II shows one major and one minor spot on autofluorography, indicating a relatively strong site-specificity of the enzyme towards MBP. At maximum, the enzyme can methylate 4.9 mole per cent of the MBP. Unlike protein methylase I [EC 2.1.1.23] which methylates arginine residues of MBP, protein methylase II activity in the brains of jimpy mutant mice (one of the dysmyelinating mutants) does not differ from the value of their normal littermates.
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태아 쥐와 임신 쥐의 간에서 Protein Carboxyl Methylation
전길자,최혜정,이준승 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-
Protein carboxyl methyltransferase(EC 2.1.1.77) which has specificities for the protein containing abnormal asparty residues is investigated in lysosome, mitochondria, peroxisome in liver of fetus, pregnant rat and vacuole vesicles in beet root. The level of this enzyme in brain cytosol of normal rat was almost same as that in brain cytosol of pregnant rat. But in liver cytosol, PCM activity of fetal rat was increased 17-fold more than that of normal rat. Total enzyme activities in lysosome, mitochondria and peroxisome of fetal and pregnant rat were not changed. But the total methylatability of fetal rat were increased about 2-fold comparing to pregnant rat. Vacuole vesicles showed the enzyme activities and methylatabilities.
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錢吉子,朴恩貞 이화여자대학교 생명과학연구소 1990 생명과학연구논문집 Vol.1 No.-
녹용에서 생리 활성 물질을 찾기 위한 첫 번째 연구로 녹용중의 당지질을 분석하였다. chloroform : methanol : 0.88% KCI 용매를 사용하여 수용성 녹용 추출액 중에서 당지질을 추출 한 후 HPTLC 방법에 의해 chloroform : methanol : 0.02% CaCl_2 전개 용매 중에서 분리하여 orcinal : ferric chloride : sulfuric acid 발색시약으로 확인하였다. 그 결과 녹용에는 4 종류의 당지질이 존재하고 있음을 알 수 있었고 그 band 1은 ganglioside GD_1a로, band 3는 GM_1으로 간주된다. 녹용의 부위에 따라 분석해 본 결과 상중대가 하대보다 더 많은 당지질을 함유하고 있음을 알 수 있었다. 이 분석 방법을 이용해서 녹용이 들어 있는 생약제제를 분석해 본 결과 녹용의 존재를 확인할 수 있었다. 따라서 다른 생약 제제에 들어 있는 녹용의 존재 확인 실험에 이용될 수 있다. In this study, the composition of glycolipids in deer antler was investigated. All nonpolar glycolipids were extracted by chloroform: methanol : 0.88% KCI misxture from the aqueous extract of deer antler. Most glycolipids including gangliosides appeared as blue zones on a light background of TLC plate by spray reagent, orcinol-ferric chloride-sulfuric acid. The aqueous extract of antler contained 4 kinds of glycolipids and among them band 1, 3 weresuggested to be GD_1a and GM_1. The top and middle parts of antler contained more glycolipids than the bottom part. The Chinese natural medicines, such as Ginseng Antlertonic Extract and Ginseng Astragali Antler Extract, were confirmed to contain antler by this method. This method can be used for the identification of existence of antler in natural medicines containing deer antler.
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