배양된 사람 치주인대세포와 골수유래간엽줄기세포의 분화에 미치는 법랑기질유도체 (Enamel Matrix Derivative, EMD)의 영향

박상규,주성숙,권용대,최병준,김영란,이백수,Park, Sang-Gyu,Jue, Seong-Suk,Kwon, Yong-Dae,Choi, Byung-Joon,Kim, Young-Ran,Lee, Baek-Soo 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.4

Introduction: Enamel matrix derivative (EMD) is a protein which is secreted by Hertwig root sheath and plays a major role in the formation of cementum and attachment of peridontium. Several studies have shown that EMD promoted the proliferation and differentiation of preosteoblasts, osteoblasts and periodontal ligament cells in vitro: however, reports showing the inhibition of osteogenic differentiation by EMD also existed. This study was designed to simultaneously evaluate the effect of EMD on the two cell lines (human mesenchymal stem cells: hMSC, human periodontal ligament derived fibroblasts: hPDLCs) by means of quantitative analysis of some bone related matrices (Alkaline phosphatase : ALP, osteopontin ; OPN, osteocalcin ; OC). Materials and Methods: hMSCs and hPDLCs were expanded and cells in the 4${\sim}$6 passages were adopted to use. hMSc and hPDLCs were cultured during 1,2,7, and 14 days with 0, 50 and 100 ${\mu}g/ml$ of EMD, respectively. ALP activity was assessed by SensoLyte ALP kit and expressed as values of the relative optical density. Among the matrix proteins of the bony tissue, OC and OPN were assessed and quantification of these proteins was evaluated by means of human OC immunoassay kit and human OPN assay kit, respectively. Results: ALP activity maintained without EMD at $1,2^{nd}$ day. The activity increased at $7^{th}$ day but decreased at $14^{th}$ day. EMD increased the activity at $14^{th}$ day in the hPDLCs culture. In the hMSCs, rapid decrease was noted in $7^{th}$ and $14^{th}$ days without regard to EMD concentrations. Regarding the OPN synthesis in hPDLCs, marked decrease of OPN was noted after EMD application. Gradual decrease tendency of OPN was shown over time. In hMSCs, marked decrease of OPN was also noted after EMD application. Overall concentration of OPN was relatively consistent over time than that in hPDLCs. Regarding the OC synthesis, in both of hPDLCs and hMSCs, inhibition of OC formation was noted after EMD application in the early stages but EMD exerted minimal effect at the later stages. Conclusion: In this experimental condition, EMD seemed to play an inhibitory role during the differentiation of hMSCs and hPDLCs in the context of OC and OPN formation. In the periodontium, there are many kinds of cells contributing to the regeneration of oral tissue. EMD enhanced ALP activity in hPDLCs rather than in hMSCs and this may imply that EMD has a positive effect on the differentiation of cementoblasts compared with the effect on hMSCs. The result of our research was consistent with recent studies in which the authors showed the inhibitory effect of EMD in terms of the differentiation of mineral colony forming cells in vitro. This in vitro study may not stand for all the charateristics of EMD; thus, further studies involving many other bone matrices and cellular attachment will be necessary.

수서생태계의 구조, 에너지 흐름과 물질순환에서 필수지방산의 역할

박상규,Park, Sang-Gyu 한국통합생물학회 2004 Korean journal of biological sciences Vol.8 No.S

생물학적 동등성 시험의 추가시험에 대한 통계적 고찰

박상규,남봉현,정윤노,이재영,정규진,Park, Sang-Gue,Nam, Bong-Hyun,Chung, Yun-Ro,Lee, Jae-Young,Jeong, Gyu-Jin 한국통계학회 2010 Communications for statistical applications and me Vol.17 No.1

제제간의 생물학적 동등성을 입증하는데 실패했을 때 추가시험을 허용하는 새로운 생물학적 동등성 시험 기준이 2008년 7월부터 시행하게 되었다. 추가시험의 의미나 시험 기준에서 제시하는 절차를 살펴보고 추가시험의 통계학적 의미를 고찰한다. 또한 사례를 통해 추가시험을 고려할 경우 어떤 점에 유의하여야 성공 할 수 있을 것인가 논의한다. The newly revised bioequivalence guideline of Korea allows the add-on test since July 1, 2008 and some discussion from statistical point of view would be needed for a practical use. The statistical model of add-on test is introduced and its two stage testing procedures are discussed. Meaningful statistical points of the add-on test are delivered through an illustrated example.

컬러 정지화 코덱 구현 연구

박상규,신현식,Park, Sang-Gyu,Shin, Hyun-Sik 한국전자통신연구원 1992 전자통신 Vol.14 No.1

ISO/IEC JTC1/SC29/WG10 JPEG(Joint Photographic Expert Group)에서는 5년여의 활동 결과로서 지난해 Grey/Color 정지화 코딩 방식에 대한 규격 (DIS 10918) 을 완성하였다. 이 규격에 포함된 코딩 방식중에서 Baseline DCT 코딩 방식은 Mandatory 코딩 방식으로서 규격을 따르는 모든 인코더/디코더가 갖추고 있어야 할 기능이다. 본 고에서는 Baseline DCT 코딩 방식을 개인용 컴퓨터에서 범용 DSP를 이용하여 구현할 수 있는 코덱 시스팀을 제시하고, 그에 따른 성능을 분석하였다.

시도로 포장관리체계(PMS) 구성요소 최적화 연구 (안산시 Prototype PMS 개발사례)

박상규,서영찬,Park, Sang-Kyu,Suh, Young-Chan 한국도로학회 2000 한국도로포장공학회 논문집 Vol.2 No.4

우리나라 도로중 PMS가 구축된 도로는 현재, 고속도로와 국도가 있다. 그러나 많은 시(市)에서 도로의 효율적인 관리를 위해 요구되는 시도로 PMS는 아직 없는 실정이다. 따라서, 본 논문에서는 시단위에 적용가능한 Prototype PMS 전산시스템을 개발 제시하였다. 개발한 PMS는 기본적으로 PC 수준에서 운영 가능하도록 구성하였으며 GIS 기능을 이용하여 사용자가 쉽게 포장상태나 유지보수 현황 및 계획을 이해할 수 있게 하였다. 본 PMS의 기능상 구성은 크게 조회, 분석, 자료관리, 출력 부분으로 구축하였다. 특히, 분석과정의 주논리(보수우선순위결정, 보수공법결정)부분은 필요시 사용자가 각 항목의 criteria 값을 수정 할 수 있게 하여 시스템운영이 경직되지 않도록 배려하였다. PMS 데이터베이스의 항목은 시도로 PMS의 규모 및 목적을 고려하여 선정하였다. 또한 PMS에서 사용된 도로 관리길이 및 위치 지정체계는 노선을 기준으로 기본 100m길이의 section으로 분할관리하였으며, 위치 지정체계 방식은 reference points 방식을 기본으로 사용하였다. PMS(Pavement Management System) has been developed and/or operated for the freeway and national highway systems in Korea. There is, however, no municipal PMS that is necessary in many cities for efficient management of their roadways. In this study, a municipal Prototype PMS is developed. The Prototype PMS is designed to be operated on PC base. It uses GIS interface for user friendliness that applies color graphics, text and distress pictures to display pavement conditions and rehabilitation information for both current and historical situations. The computer program of the prototype PMS consists of four major functions(inquiry, analysis, data management, and print). Especially, prioritization and M&R(Maintenance and Rehabilitation) alternative selection procedure is designed to be flexible so that the users can modify the decision criteria whenever it is necessary. Database items of the Prototype PMS is carefully selected for the objectives and size of municipal PMS. Reference point method is basically used for the location referencing in the Database. The unit section consists of each loom segment of the road for effective management.

산소 전달 특성에 미치는 이젝터 구동 노즐 면적비에 따른 혼합 분류의 영향

박상규,양희천,Park, Sang Kyoo,Yang, Hei Cheon 한국분무공학회 2022 한국액체미립화학회지 Vol.27 No.3

The objective of this is to experimentally investigate the effect of mixed jet on the oxygen transfer characteristics with the primary nozzle area ratio of an annular nozzle ejector for the application of a microbial fuel cell. A direct visualization method with a high speed camera system was used to capture the horizontal mixed jet images, and a binarization technique was used to analyze the images. The clean water unsteady state technique was used for the oxygen transfer measurement. The air-water mixed jet discharging into a water tank behaved similar to a buoyancy or horizontal jet with the primary nozzle area ratio. It was found that an optimum primary nozzle area ratio was observed where the oxygen transfer performance reached its maximum value due to the decrease of air volume fraction and the increase of jet length and air bubble dispersion.

한국 IT 벤처기업의 국제화 학습: 유니데이타커뮤니케이션(주) 사례

박상규,문휘창,Park, Sang-Gyu,Moon, Hwy-Chang 한국벤처창업학회 2012 벤처창업연구 Vol.7 No.1

본 사례에서는 국내 IT 벤처기업인 유니데이타커뮤니케이션(주)의 단계적인 국제화 경험이 학습역량 강화를 통해 경영성과에 기여하는 일련의 국제화 과정을 조직학습이론으로 설명하였다. 이는 조직의 국제화 경험이 국제화 성과의 중요한 결정요인이며 조직학습을 통해 배양된 학습역량은 경영성과에 기여한다는 기존 연구결과를 기반으로 하고 있다. 더불어, 창업기부터 꾸준히 국제화를 추진한 IT 벤처기업의 국제화학습 특성을 기존지식 활용(Exploitation)과 새로운 지식 탐색(Exploration)으로 구분하여 분석하였다. 분석결과, 대기업에 비해 내부자원이 취약한 벤처기업은 파트너 네트워크를 활용한 탐색과 활용을 통해 새로운 국제화 경험을 축적하였다. 축적된 국제화 경험은 학습 역량 강화를 통해 기업의 내부역량으로 배태(embedded)되어 궁극적으로 경영성과에 유의미하게 작용함을 사례를 통해 확인 할 수 있었다. This paper explains the contribution of international learning to the business performance through strengthening the international learning capability of the organization. This is consistent with the previous literature that the organization's international experience is an important determinant of the international performance and the learning capability, strengthened through organizational learning, contributes to the business performance. In addition, this paper analyzes the characteristics of international learning of a venture business, which has actively pursued internationalization, by categorizing exploitation and exploration. An important result is that the small-sized venture firm, which is weak in internal resources compared with large firms, utilizes partner network more actively to accumulate international experience by exploration. We can then confirm that this international experience strengthens learning capability and gives positive effects on the business performance.

The 4^th.industrial revolution and Korean university's role change

박상규,Park, Sang-Kyu Convergence Society for SMB 2018 융합정보논문지 Vol.8 No.1

최근 각 언론, 기업계, 정부 유관기관 및 학계 등 많은 분야에서 4차 산업에 대한 관심이 폭발적으로 증가하였다. 특히 우리가 피부로 느낄 수 있는 분야인 인공지능이 인간능력을 이미 크게 앞서고 있다는 것을 깨닫고 나서 많은 사람들은 4차산업혁명이 실제로 우리 코 앞에 와있다는 것을 실감할 수 있었다. 이렇게 대부분 사람들의 생각보다 빠르게 다가온 4차산업을 어떻게 효율적으로 대응해야 할까? 특히 최근의 인공지능, 빅데이터, 무인자동차 및 유전자가위 등에 대한 상반된 견해들을 비교분석하는 방식으로 연구를 진행해 본다. 이러한 분석과 연구를 통하여 교육적, 정치적, 사회적, 윤리적 그리고 과학적 영향들을 파악해 본 결과, 현재까지 뚜렷하게 정립되어 있는 개념이나 체계, 시스템이 존재하지 않는다는 것을 이해할 수 있었고 오히려 4차산업혁명의 개념, 체계를 앞서서 정의하고 정립하는 국가나 기업, 개인들이 산업의 주도권을 확보할 수 있다는 것을 알게 되었다. 그러나 한국사회와 대학은 오히려 현재 2차산업혁명의 체계와 문화에서 머물러있는 듯한 모습을 보이고 있는데, 이러한 현실인식 위에서 새로운 산업혁명의 트렌드를 맞추어 따라갈 수 있는 방안들을 찾아 보고자 한다. The interest about 4th Industrial Revolution was impressively increased from newspapers, iindustry, government and academic sectors. Especially AI what could be felt by the skin of many peoples, already overpassed the ability of the human's even in creative areas. Namely, now many people start fo feel that the effect of the revolution is just infront of themselves. There were several issues in this trend, the ability of deep learning by machine, the identity of the human, the change of job environment and the concern about the social change etc. Recently many studies have been made about the 4th industrial revolution in many fields like as AI(artificial intelligence), CRISPR, big data and driverless car etc. As many positive effects and pessimistic effects are existed at the same time and many preventing actions are being suggested recently, these opinions will be compared and analyzed and better solutions will be found eventually. Several educational, political, scientific, social and ethical effects and solutions were studied and suggested in this study. Clear implication from the study is that the world we will live from now on is changing faster than ever in the social, industrial, political and educational environment. If it will reform the social systems according to those changes, a society (nation or government) will grasp the chance of its development or take-off, otherwise, it will consume the resources ineffectively and lose the competition as a whole society. But the method of that reform is not that apparent in many aspects as the revolution is progressing currently and its definition should be made whether in industrial or scientific aspect. The person or nation who will define it will have the advantage of leading the future of that business or society.

PCR 과 Southern hybridization 을 이용한 구지뽕나무와 무궁화의 클론감별

박상규,류장발 ( Sang Gyu Park,Jang Bal Ryu ) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.1

Polymerase chain reaction (PCR) and Southern hybridization analyses were carried out to identify clones of silk worm thorn (Cudraria tricuspidata) and Rose of sharon (Hibiscus syriacus) which look like one tree with two ar three, branches or two or three different trees. For PCR five different PCR primers (17∼24 nucleotides) are derived from CaMV 35S promoter, nopaline synthase terminator and coding region of thylakoid membrane protein gene. In the case of silk worm thorn, about 500 bp of PCR product was produced from DNAs of one tree or branch in the presence of 35S primer alone. Southern hybridization analysis of genomic DNAs hybridized with ^(32)P labeled PCR product showed that the same size of DNA fragments were hybridized with different intensities. In addition, PCR analyses using 20 different primers of OPERON 10-mer kits showed that only OPA01 primer produced PCR products of different size. These results indicate that two different trees of silk worm thorn combined to one tree. In the case of the Rose of Sharon, the same size of PCR products were produced from three different samples but Southern hybridization with the above PCR product as a probe did not show any hybridized bands. PCR analyses in the presence of OPERON 10-mers showed OPA04 and OPA13 produced different products including same sizes of products. These, results indicate that three different trees of the Rose of Sharon seem to be derived from the tree.

Rand tests for Comparing several treatments with a control in a Randomized Block experiment

박상규,김정일,이은구 ( Sang Gue Park,Jeong Il Kim,Eun Koo Lee ) 한국품질경영학회 1991 품질경영학회지 Vol.19 No.1