고지방식이에 의한 흰쥐의 시상하부 Neuropeptide Y-면역반응 신경세포와 장내 Cajal 세포의 변화

문지영,문경래,박상기,정윤영,김은영,Moon, Ji-Young,Moon, Kyung-Rae,Park, Sang-Kee,Chung, Yoon-Young,Kim, Eun-Young 대한소아소화기영양학회 2011 Pediatric gastroenterology, hepatology & nutrition Vol.14 No.2

목 적: 고지방식이를 섭취한 흰쥐에서 식이 유발인자인 NPY와 장내 운동 조절인자인 Cajal 간질세포의 변화를 관찰하고자 하였다. 대상 및 방법: 동일 연령의 Sprague-Dawley계 수컷 흰쥐를 임의로 대조군(7마리)과 고지방식이군(7마리)으로 분리한 후, 대조군에는 일반 흰쥐 사료를 충분히 섭취시켰으며, 고지방식이군에게는 60% kcal 지방이 포함된 사료(중앙실험동물센터)를 6주 동안 물과 함께 충분히 섭취하도록 하였다. 6주 후 시상하부 조직 절편에 rabbit polyclonal NPY (1 : 1000, Abcam)항체를 반응시키고, 소장의 조직 절편에 rabbit polyclonal c-kit (1 : 400, Neuromics)항체를 반응시켜서 면역조직화학 검사를 시행하였다. 결 과: 고지방식이군의 시상하부에서 NPY의 면역 반응성이 강하게 나타났으며, 특히 PVN에서 NPY의 면역 반응 세포수의 증가를 관찰할 수 있었다. 고지방식이군에서 소장의 Auerbach's plexus 부위의 Cajal 간질 세포의 면역 반응성이 떨어졌으며, 환상근과 종주근 내 Cajal 간질 세포 수도 현저히 감소하였다. 결 론: 고지방식이를 섭취한 흰쥐에서 시상하부 NPY 면역반응 신경세포수와 발현의 증가는 NPY의 저항성과 관련되며, 소장에서 Cajal 간질 세포의 감소는 장운동의 이상을 초래할 수 있음을 나타낸다. Purpose: The aim of this study was to assess changes in neuropeptide Y (NPY) immunoreactivity in the hypothalamus and interstitial cells of Cajal (ICC) in the small intestine of rats fed high-fat diets (HFD). Methods: Male Sprague-Dawley rats (200~250 g body weight) were randomly divided into two groups, which were the control group (normal chow diet for 6 weeks), and the HFD group (rodent diet with 60% kcal fat for 6 weeks). The immunoreactivity of NPY in the hypothalamus and ICC in the small intestine was evaluated after every feed for 6 weeks. Results: NPY immunoreactivity was observed strongly in the hypothalamic nuclei in the HFD group compared to the control group. The numbers of NPY-immunoreactive (IR) cells were significantly higher in the paraventricular hypothalamic nucleus in the HFD group than in the control group. In the region of Auerbach's plexus (AP) of small intestine, the staining intensity of the ICC-IR cells was reduced in the HFD group compared to the control group. The numbers of ICC in the small intestine with HFD, including ICC in the inner circular and outer longitudinal muscle were significantly lower than in the control group. Conclusion: This study suggested that increasing NPY-IR cells in the hypothalamus may reflect resistance of NPY action after a HFD, and decreasing ICC-IR cells in the small intestine after a HFD is functionally significant in gastrointestinal motility.

소아 비만에서 Leptin과 골대사의 연관성

김은영,노영일,양은석,문경래,박상기,박영봉,이영화,Kim, Eun Young,Rho, Young il,Yang, Eun Seok,Moon, Kyung Rae,Park, Sang Kee,Park, Yeong Bong,Lee, Young Hwa 대한소아소화기영양학회 2006 Pediatric gastroenterology, hepatology & nutrition Vol.9 No.2

목 적: 체중과 지방량이 골밀도와 밀접한 연관이 있는 것으로 알려져 왔다. 비만아에서는 지방 조직에서 합성 분비되는 leptin이 증가되는데, 증가된 leptin이 비만아의 골 대사에 미치는 영향에 대해 알아보고자 연구를 하였다. 방 법: 2005년 11월부터 2006년 5월까지 조선대학교병원 외래를 방문한 50명의 남자(비만아 25명, 정상 체중의 대조군 25명)를 대상으로 하였다. 체질량 지수, 체지방률, 삼두박근 피부주름 두께와 견갑골하 피부주름 두께를 측정하고 혈청 leptin과 골대사 지표인 B-ALP, CICP 및 total DPD 농도를 검사하였다. 비만군과 대조군의 신체 계측, leptin 및 골대사 지표 농도를 비교하고, leptin과 골대사 지표와의 상관성을 알아보았다. 결 과: 비만군에서 체질량 지수(r=0.551, p<0.05), 체지방률(r=0.584, p<0.05)과 유의한 양의 상관관계가 있었다. Leptin은 골형성 지표인 B-ALP (r=-0.613, p=0.026) 및 CICP (r=-0.583, p=0.037)와 유의한 음의 상관성을 보였다. 그러나 골흡수 지표인 total DPD (r=0.103)와는 유의한 상관성이 없었다. 대조군에서 leptin과 유의한 상관성이 있는 인자는 없었다. 골대사에 영향을 주는 체중을 통제하고 상관성을 알아본 결과에서도 비만군에서 leptin은 B-ALP (r=-0.728, p=0.007) 및 CICP (r=-0.684, p=0.014)와 유의한 음의 상관성을 보였으나 total DPD와는 연관성이 없었다. 대조군에서는 leptin과 연관성 있는 골대사 지표는 없었다. 비만군에서 골대사 지표에 영향을 미치는 인자에 대해 다중 단계별 회귀 분석을 시행한 결과 종속변수인 B-ALP는 leptin과 유의한 음의 상관성을 보였으며(Y=-39.653X+356.341, p=0.026), CICP도 leptin과 유의한 음의 상관성을 보였다(Y=-13.437X+116.013, p=0.037). 결 론: 비만아에서 leptin은 골형성 지표인 B-ALP 및 CICP와 음의 상관성이 있었으며, 골 흡수가 아닌 골 형성을 저하시키는 것을 알 수 있었다. Purpose: The aim of this study was to evaluate the influence of leptin on biochemical markers of bone metabolism in childhood obesity. Methods: A total of 50 male children (25 obese and 25 controls) were recruited from the pediatric outpatient clinic at the Chosun University Hospital from November 1st 2005 to May 30th 2006. BMI, body fat percentage, serum leptin, bone-specific alkaline phosphatase (B-ALP), C-terminal propeptide of type 1 collagen (CICP), total deoxypyridinoline crosslinks (total DPD) were measured. The correlations of leptin with BMI, body fat percentage, B-ALP, CICP, total DPD were analyzed by Pearson's correlation. In a multiple stepwise regression analysis, leptin after correction for body weight was evaluated if there was a correlation with biochemical markers of bone formation and resorption respectively. Results: The leptin levels of the obese group were significantly higher than those of the control group (p=0.012). In the obese group, the leptin level was significantly positively correlated with the BMI (r=0.551, p=0.01) and the percentage of body fat (r=0.584, p=0.018). In the obese group, of bone markers, B-ALP (r=-0.613, p=0.026) and CICP (r=-0.583, p=0.037) were negatively correlated with leptin. B-ALP (r=-0.728, p=0.007) and CICP (r=-0.684, p=0.014) were negatively correlated with leptin when corrected for body weight. In the control group, bone markers were not correlated with leptin. In the multiple stepwise regression analyses, there was a negative correlation between the leptin and B-ALP (Y=-39.653X+356.341, p=0.026), CICP (Y=-13.437X+ 116.013, p=0.037) respectively in the obese group. Conclusion: Leptin was a significant factor in the bone formation but not in bone resorption in childhood obesity.

양수내에서 황색포도상구균의 증식억제 -트렌스페린 결합 단백에 대한 항체-

신성희 ( Sung Heui Shin ),양은석 ( Eun Suk Yang ),박상기 ( Sang Kee Park ),문경래 ( Kyung Rae Moon ),임용 ( Yong Lim ),전호종 ( Ho Jong Jeon ),김진호 ( Jin Ho Kim ) 대한주산의학회 2000 Perinatology Vol.11 No.4

복합운동프로그램이 비만중년여성들의 생활습관병 관련인자와 혈중활성산소에 미치는 영향

양승자(Seung-Ja Yang),서영환(Young-Hwan Seo),오은해(Eun-Hae Oh),문경래(Kyung-Rae Moon) 한국발육발달학회 2018 한국발육발달학회지 Vol.26 No.2

Recently, due to lack of physical activity, middle-aged women are exposed to Lifestyle-related diseases by obesity and health care is urgently needed. The purpose of this study was to investigate the effect of complex exercise program on Lifestyle-related factors and blood free oxygen radical of middle-aged obese women. The two-way repeated measure ANOVA was used to verify the difference between the time of measurement and the group. At this time, the paired sample t-test was performed for the variables with statistically significant differences in each variable. The significance level of all results was set to p<.05 and the following conclusions were obtained. In the changes of Lifestyle-related factors, the body fat percentage showed an interaction effect between group and period & group. The blood glucose showed only the interaction effect between period & group. Total cholesterol had an interaction effect between timing and period & group, In neutral fat, there was only interaction effect between timing & group. In blood pressure, there was no interaction effect in time, group and time & group. Also in all the factors of the exercise group, the difference between the two groups was statistically significant after the experiments than before. Changes in blood activated oxygen were found to have an interaction effect between the two groups. In the exercise group, statistically significant decrease was observed after the experiment. In conclusion, complex exercise has a positive effect on improving Lifestyle-related factors and blood stress. This indicates that this can be a recommended exercise method for middle-aged obese women. In future follow-up studies, if the exercise duration is prolonged and the intensity is gradually increased, it can be considered to be a program that can prevent various Lifestyle diseases by eliminating middle-aged obesity.

여성독거노인의 건강관련 삶의 질과 보완대체의학 이용 및 의도와의 관련성

박선미(Park Sun Mi),한미아(Han Mi Ah),박종(Park Jong),이미자(Lee Mi Ja),문경래(Moon Kyung Rae),최송실(Choi Song Sil) 한국노년학회 2014 한국노년학 Vol.34 No.4

Purpose: This study aimed to identify association between the health-related quality of life and complementary and alternative medicine (CAM) in elderly women living alone. Method: A face-to-face survey with each elderly who lives alone in Gwangju, was proceed for two months from October to September, 2013, and 478 questionnaires were analyzed for this study. General characteristics, complementary and alternative medicine (CAM) related factors and health related quality of life (HRQoL: EQ-5D) were collected. Chi-squre tests and multiple logistic regression were tested to evaluate association between HRQoL and CAM use. We performed multiple logistic regression to identify whether factor of life quality was independently meaningful variables to CAM use after controlling the confounding variables. Result: A total of 478 subjects, and 39.7% were more than 80 years. About 51% elderly women lived alone for more than 15 years. The pain/discomfort dimension was the highest reported problem: 85.6% for elderly women. The proportion of CAM use was significantly higher among elderly with problem in mobility compared to elderly without problem (OR=2.74, 95% CI=1.45-5.18). The proportion of CAM use intention was significantly higher among elderly with problem in mobility (OR=1.97, 95% CI=1.06-3.66) and pain/discomfort (OR=2.21, 95% CI=1.07-4.55). Conclusion: The HRQoL was significanlty associated with CAM use in elderly women living alone. Further study is needed to investigate the role of CAM in elderly considering elderly's medical conditions. 본 연구는 광주지역의 65세 이상 여성독거노인에서 건강관련 삶의 질의 영역과 보완대체의학 이용과의 관련성을 알아보고자 하였다. 2013년 9월부터 10월까지 광주지역 독거노인을 대상으로 각 구당 100명씩 총 500명을 대상으로 1:1면접조사를 시행하였으며, 그 중 여성 독거노인 478명을 대상으로 하였다. 조사원은 사전 집체교육을 통해 표준화된 방식으로 조사를 진행할 수 있도록 하였으며, 설문도구를 활용하여 조사하였고, 빈도분석, 카이제곱 검정, 다중로지스틱 회귀분석을 이용하여 분석하였다. 연구결과를 살펴보면, 대상자의 39.7%가 80세 이상이었으며, 약 51%는 독거기간이 15년 이상이었다. 여성독거노인의 85.6%는 통증/불편에 지장이 있었으며, 운동능력에 지장이 있는 경우 보완대체의학 이용여부(OR=2.74, 95% CI=1.45-5.18)와 이용의도(OR=1.97, 95% CI=1.06-3.66)가 유의하게 증가하였고, 통증/불편에 지장이 있는 경우 보완대체의학 이용여부가 유의하게 증가하였다(OR=2.21, 95% CI=1.07-4.55). 결론적으로 광주지역 여성독거노인에서 건강관련 삶의 질과 보완대체의학이용여부 또는 이용의도와의 관련성을 파악할 수 있었다. 향후 의학적 처치를 고려한 보완대체의학의 적절한 사용에 대한 연구가 필요하리라 판단된다.

소아 백혈병의 혈청 철, 총 철결합능 Transferrin포화율 및 Ferritin치에 관한 연구

문경래,정은경,박상기 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

Author reviewed the clinical symtoms, hematological findings and serum Fe, total iron binding capadty(TIBC), transferrin saturation and levels of 40 children diagnosed as leukemia at the pediatric department of Chosun University hospital during a period of 7 years from Jan. 1983 to Dec. 1989. Also, 20 normal children as control group were studied. The results were summarized as follows: 1) The Mean±S.D. levels of the serum Fe, TIBC, transferrin saturation and ferrtin of leukemia 40 cases and control group were 126.8±37.8㎍/㎗, 276.1±37.8㎍/㎗, 47.1±15.6%, 244.5±52.7ng/㎖ and 107.2±34.2㎍/㎗, 309.9±31.8㎍/㎗, 34.7±11.4%, 69.6±30.8ng/㎖, respectively. The difference of the serum Fe levels between leukemia and normal control was statistically not significant (P>0.1) but the serum Fe, TIBC, transferrrin saturation and ferritin levels were significantly different(P<0.05, P<0.01, P<0.001). 2) The differences in the mean levels of the serum Fe, TIBC, transferrin saturation and ferfitin among the types of leukemia were not significant. 3) The differences of the serum TIBC, transferrin saturation and ferritin levels between leukemia and normal control were statistically significant. From the very high serum ferritin levels, it is thought that serum ferritinemia as tumor marker may be useful in diagnosis of leukemia.

학동기 비만아동의 피부두께의 통계적 관찰

김경석,나기찬,정은경,문경래,박상기,박영봉 朝鮮大學校 附設 醫學硏究所 1993 The Medical Journal of Chosun University Vol.18 No.1

The measurements of triceps skinfold thickness, body weight and height were done in children aged 7 to 12 years(3,142 boys and 2588 girls) in Kwangju in 1992. The author also observed degree of obesity, prevalence of obesity and average value of triceps skinfold thickness. The results were summarized as follows: 1) The mean value of triceps skinfold thickness acceding to age in male was 7 years 10.5mm, 8 years 10.5mm, 9 years 10.2mm, 10 years 12.3mm, 11 years 13.8mm, 12 years 12.3mm. In the female it was 7 years 11.9mm, 8 years 11.9mm, 9 years 11.6mm, 10 years 12.6mm, 11 years 14.1mm, 12 years 13.6mm. 2) The mean value of triceps skinfold thickness acceding to age in obese male was 7 years 16.3mm, 8 years 17.3mm, 9 years 16.0mm, 10 years 19.5mm, 11 years 23.7mm, 12 years 20.1mm. In the obese female it was 7 years 17.3mm, 8 years 17.9mm, 9 years 18.4mm, 10 years 19.5mm, 11 years 21.2mm, 12 years 20.3mm.

도시지역 초등학생에서 주의력결핍 과잉운동장애의 유병율

표경식,박상학,김상훈,조용래,김학렬,문경래 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.2

Background and Objectives : To study the prevalence of ADHD in urban elementary school children using the Korean version of the disruptive behavior disorders parent rating scale. Materials and methods : 6,372 students from seven urban elementary schools were targeted using the parent rating scale. Results : (1)The total ADHD prevalence was 6.1%. It can be broken down as follows: 4.1% was the Inattentive type, 1.0% was the Hyperactive-impulsive type and 1.1% was the Combined type. 8.3% occurred in male students and 3.9% occurred in female students. The prevalence in boys was significantly higher. (2) The prevalence of Conduct Disorder(CD) was 0.3%: 0.2% for male students and 0.3% for female students. There was no significant difference between girls and boys. (3) The prevalence of Oppositional Defiant Disorder(ODD) was 4.4%: 5.0% for boys and 3.9% for girls. The value for boys was significantly higher. (4) A statistically significant difference between grades was found with ADHD, but no significant difference between grades was found with CD and ODD. (5) In the results of the comorbidity research, ADHD accompanied by CD was 2.3%. ADHD accompanied by ODD was 25.5%. ADHD accompanied by both CD and ODD was 4.1%. (6) Mild forms of ADHD, CD and ODD were found four times more often than that observed in typical cases. Conclusion : Prevalence of ADHD in urban elementary schools is high and CD or ODD is accompanied more frequently. In the future, the school mental health project should be focused mainly on ADHD. From the beginning of urban elementary schools, ADHD should be screened for. Intervention is required depending on whether or not there are accompanying diseases.

무균성 뇌막염에 있어 뇌척수액 추적검사에 대한 재평가

정은경,김경석,김갑승,문경래,박상기 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

The author evaluated the clinical manifestation of forty cases of aseptic meningitis who were admitted in the pediatric department of the Chosun University Hospital from March 1990 to September 1990, and also observed the clinical course of two groups of aseptic meningitis divided by admitting time and reevaluated the needness of follow-up lumbar puncture for CSF examination. The author performed follow-up lumbar puncture for CSF examination to the first group, on the 7th hospital day by conventional way, otherwise only observed the clinical course without follow-up lumbar puncture for CSF examination to the 2nd group. There was no difference between both groups, and clinical improvement was seen in both groups. In conclusion, the author considers that if clinical and laboratory finding were compatible with aseptic meningitis, there may be no need for follow-up lumbar puncture for CSF exmenation.

모유 수유아와 조제 분유 영양아에서 수유 방법에 따른 아미노산 농도 비교 및 혈청 트립토판 농도와 수면 유도와의 관계

정다운,김은영,양은석,박상기,박영봉,문경래 朝鮮大學校 附設 醫學硏究所 2005 The Medical Journal of Chosun University Vol.30 No.2

Objective: Sleep behavior is modulated by serotonergic neurons within the brain, and the synthesis and release of serotonin by such neurons is thought to be influenced by the availability of tryptophan, the amino acid precursor of serotonin. Formula-feeding infants have depressed plasma tryptophan concentration relative to breast milk-feeding infants. Because tryptophan alters sleep waking patterns in adults, a study was designed to determine the difference of sleep latency relative to differences in plasma tryptophan and tryptophan : large neutral amino acid (LNAA) ratio between formula-feeding infants and breast milk-feeding infants. Method: 45 newborns who were born in Chosun University Hospital from December 2002 to December 2003 were selected. The newborns were divided to three group, 15 newborns were fed breast milk and 15 newborns were fed formula A, and last 15 newborns were fed formula B. At 6 weeks and 12 weeks, infants were sampled for measuring of serum amino acid level and tryptophan, tryptophan : LNAA ratio. And we taught infants' parents to measure sleep latency that means the time after a feeding to the first episode of active REM sleep that persist ≥ 1 min. Result: 1) At 6weeks, Serum α-amino-n-butyric acid, citrulline, tryptophan level was higher in formula A group than breastmilk group (P<0.05) and proline level was lower than breast milk group (P<0.05). In formula B group, serum n-amino-n-buryric acid, citrulline, cystine, hydroxylysine, hydroxyproline, isoleucine, leucine, lysine, methionine, phenylalanine, taurine, threonine, valine levels were higher than breastmilk group (P<0.05). And Serum isoleucine, methionine, proline, valine level were higher in formula B group than formula A group (P<0.05). 2) At 12 weeks, serum glutamic acid, methionine, ornithine levels were higher in formula group A than breast milk group (P<0.05). Serum β-alanine, aspartic acid, α-amino-n-butyric acid, ethanolamine, glutamic acid, threonine level were higher in formula B group than breast milk group (P<0.05). Serum β-alanine, ethanolamine levels were higher and arginine level was lower in formula B group than formula A (P<0.05). 3) At 6 weeks, serum tryptophan concentration and tryptophan: LNAA ratio were higher in formula A group (P<0.05). Sleep latency was 21 minutes in formula group A, 24 minutes in breast milk group, 25 minutes in formula B group but there was no statistically significance (P>0.05). 4) At 12 weeks, serum tryptophan concentration and tryptophan: LNAA ratio, time after feeding to the first episode of active REM sleep were no difference with each other group. Conclusion: There is significant difference of serum amino acids between breast-feeding infants and formula-feeding infants. And serum tryptophan and tryptophan: LNAA ratio differ between brest-feeding infants and formula-feeding infants. But there is no association between serum tryptophan, tryptophan: LNM ratio and sleep latency.