Phenotypic Characterization and ln Vivo Localization of Human Adipose-Derived Mesenchymal Stem Cells

류영준,조재진,조태준,이동섭,최진영 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.6

Human adipose-derived mesenchymal stem cells (hADMSCs) are a potential cell source for autologous cell therapy due to their regenerative ability. However, detailed cytological or phenotypic characteristics of these cells are still unclear. Therefore, we determined and compared cell size, mor-phology, ultrastructure, and immunohistochemical (IHC) expression profiles of isolated hADMSCs and cells located in human adipose tissues. We also characterized the localization of these cells in vivo. Light microscopy examination at low power revealed that hADMSCs acquired a spindle-shaped morphology after four pas-sages. Additionally, high power views showed that these cells had various sizes, nuclear contours, and cytoplasmic textures. To further evaluate cell morphology, transmission electron microscopy was performed. hADMSCs typically had ultrastructural characteristics similar to those of primitive mesenchymal cells including a relatively high nuclear/cytosol ratio, prominent nucleoli, immature cytoplasmic organelles, and numerous filipodia. Some cells contained various numbers of lamellar bodies and lipid droplets. IHC staining demonstrated that PDGFR and CD10 were constitutively expressed in most hADMSCs regardless of passage num-ber but expression levels of -SMA, CD68, Oct4 and c-kit varied. IHC staining of adipose tissue showed that cells with immunophenotypic characteristics identical to those of hADMSCs were located mainly in the peri-vascular ad-ventitia not in smooth muscle area. In sum-mary, hADMSCs were found to represent a heteroge-neous cell population with primitive mesenchymal cells that were mainly found in the perivascular adventitia. Furthermore, the cell surface markers would be CD10/PDGFR. To obtain defined cell populations for therapeutic purposes, further studies will be required to establish more specific isolation methods.

韓國歷代政權의 國家目標設定과 그 政治的 課題

劉英俊 한국정치학회 1980 한국정치학회보 Vol.14 No.-

트립티센 기반의 트리페닐아민 전자-주게 분자 합성 및 특성 분석

류영준,안병관 한국전기전자재료학회 2022 전기전자재료학회논문지 Vol.35 No.4

The development of efficient electron donor (or hole-transporting) molecules that can be used in variousoptoelectronic device fields is highly demanded. In this work, a novel class of triptycene-based three-dimensional (3D)triphenylamine (TI-TPA) derivatives with different end substituents was designed and prepared for transparent electron donor materials. Owing to the rigid 3D triptycene framework, the obtained TI-TPA derivatives had an amorphous morphology with high thermal decomposition temperature. The oxidation potential of these TI-TPA derivatives decreased as the electron donating strength of the end substituent increased. Among TI-TPA derivatives, TI-TPA-OMe exhibited the highest HOMO level (-5.31 eV) which is similar to that of Spiro-OMeTAD (-5.22 eV). In addition, TI-TPA-OMe was found to form a strong charge transfer complex with the triptycene-based acceptor TI-BQ, leading to a new absorption band at around 640 nm. These results can be applied for developing efficient electron donor materials that can mimic the advantages of the spiro-linked structure and TPA units of Spiro-OMeTAD.

Nylon6 Non-Woven with Nano Fiber

류영준,강영식,김학용,이덕래 한국고분자학회 2001 한국고분자학회 학술대회 연구논문 초록집 Vol.2001 No.10

Analysis of Human Tissue Management Models for Medical Research: Preparation for Implementation of the 2012 Revision of the Bioethics and Safety Act of Korea

류영준,김한겸,장세진,구영모 대한의학회 2013 Journal of Korean medical science Vol.28 No.6

Efficient management of human tissue samples is a critical issue; the supply of samples is unable to satisfy the current demands for research. Lack of informed consent is also an ethical problem. One of the goals of the 2012 revision of Korea’s Bioethics and Safety Act was to implement regulations that govern the management of human tissue samples. To remain competitive, medical institutions must prepare for these future changes. In this report, we review two tissue management models that are currently in use; model 1 is the most common system utilized by hospitals in Korea and model 2 is implemented by some of the larger institutions. We also propose three alternative models that offer advantages over the systems currently in use. Model 3 is a multi-bank model that protects the independence of physicians and pathologists. Model 4 utilizes a comprehensive single bioresource bank; although in this case, the pathologists gain control of the samples,which may make it difficult to implement. Model 5, which employs a bioresource utilization steering committee (BUSC), is viable to implement and still maintains the advantages of Model 4. To comply with the upcoming law, we suggest that physicians and pathologists in an institution should collaborate to choose one of the improved models of tissue management system that best fits for their situation.

2019 International Neuroethics Society: An Expanded Vision*— 참석 후기 —

류영준,김상현 미래의료인문사회과학회 2019 FUTURE MEDICINE & HUMANITIES Vol.2 No.2

2019 Internatioanl Neuroethics Society(INS) 정기 학술대회가 10월 17-18일 미국 시카고 Radison Blu Aqua 호텔에서 개최되었다. 이번 학술대회에서는 임상 경험이나 임상시험 연구에 기반한 실증적 연구(empirical study)에 대한 발표가 주를 이루었다. 신경학적 또는 정신적 증상에 전임상적(preclinical)으로 개입하면서 생기는 윤리적 측면에 대한 논의에서 질병에 걸릴 ‘확률적 가능성’을 알려주는 것은 의학적 혹은 통계학적 지식이 없는 일반인에게는 사실상 무의미한 정보가 될 수 있다는 지적은 매우 중요하다고 생각한다. 그리고 뇌심부자극술(DBS) 연구가 종료된 경우 연구 참여자의 사후 관리(치료 지속 혹은 기기제거 비용)에 관한 연구자들의 윤리적 책임과 규율의 흠결에 대한 지적도 의미가 있었다. 한편 뇌질환이나 정신질환에 대한 예측 가능성과 치료 가능성이 높아지는 것의 파급 효과는 환자와 병원의 범주를 벗어나고 있기도 하다. 뇌의 건강 상태의 예측 가능성은 한 개인의 법적 책임의 범위뿐만 아니라 사회경제적 취약계층에 대한 정부의 정책에도 영향을 미칠 수 있으며, 나아가 수형자의 처벌 및 교정의 방법이 변화될 가능성까지 점쳐지고 있다. 그 동안 뇌의 구조적・기능적 이상과 폭력성간의 관계에 관한 연구들은 있었으나, 실제로 수형자 연구 코호트를 구축해서 비침습적 뇌신경조절술의 폭력성 감소 효과를 연구하는 것은 매우 이례적인 것으로 새로운 접근법으로 보인다. 앞으로 이러한 연구의 윤리적 정당성과 형사정책적 효용성에 대한 많은 연구가 필요할 것이다. The 2019 International Neuroethics Society (INS) Annual Conference was held at Radison Blu Aqua Hotel, Chicago, USA, on October 17-18. In this conference, a number of presentations on neuroscientific empirical studies were mainly featured and discussed. Ethical issues of preclinical interventions of neurological or psychiatric symptoms include health information literacy of the layperson. They pointed out that there are regulatory loopholes on duties to follow up care on the participants after the termination of the DBS clinical research. Meanwhile, the advances in brain science have ripple effects in various social areas besides the medical field. The predictability of the brain health condition can affect not only the criminal responsibility, but also the governmental policy toward the vulnerable groups. Furthermore, there is a possibility that the method of punishment and correction of inmates may change. While there have been studies on the relationship between brain abnormalities and violence, running an offender cohort to study the violence-reducing effects of non-invasive brain intervention seems to be a novel approach. Further studies will be needed to verify and support the ethical justification and utility of the neuroscientific approach.

한국의 연구중심병원의 연구용 인체조직 관리체계 향상을 위한 제언

류영준,김한겸,장세진 대한의사협회 2012 대한의사협회지 Vol.55 No.3

In this paper, the authors suggest more effective conditions for human tissue preparation in research hospitals. Because recent genetic and molecular studies have contributed to the rapid development of molecular and genetic medicine, human tissue is now again being considered as a valuable research resource. Basically, high-quality research-oriented tissue bank organizations are a very important part of a research hospital. The current management system for human tissue, however, is not very effective from either legal or practical perspectives. In this article, the authors propose some improvements on the human tissue management system. The laws on human tissue such as the Bioethics and Safety Act should be changed to contain suitable language applying on-site and efficient multi-dimensional information. Informed consent should be an essential requirement before surgery. Pathologists should be supported as the essential manpower of human tissue banks by law. A committee composed of a clinician, researcher, pathologist, information manager, and coordinator should be established to manage human tissue banks in hospitals. The Institutional Review Board should pay more attention to preventing the leakage of the private information of donors, and researchers should know about the review process and guidelines. This suggestion will create a more stable and effective system for the management of human tissue banks, and it will also create a complete and integrated system for research institutions. Therefore, human tissue banks can play an important role in improving research hospitals’ competitiveness by providing a valuable collection of material for research.

Embryonal Rhabdomyosarcoma Arising from a Mediastinal Teratoma: An Unusual Case Report

류영준,유수현,정민정,장세진,조경자 대한의학회 2013 Journal of Korean medical science Vol.28 No.3

We report an unusual case of 9.5-cm-sized embryonal rhabdomyosarcoma arose from a mediastinal mature teratoma in a 46-yr-old man. A man presented with chest trauma as a result of an accident at 10 September 2011. On chest X-ray, an anterior mediastinal mass was detected. To obtain further information, chest computed tomography (CT) with contrast enhancement was performed, revealing an anterior mediastinal mass. Complete surgical excision was performed and entire specimen was evaluated. Pathologic diagnosis was embryonal rhabdomyosarcoma arising in mature cystic teratoma. After surgical excision, two cycles of dactinomycin-based chemotherapy were performed. Lung metastasis was detected on follow up CT in September 2012, and wedge resection was performed. Pathological finding of the lung lesion showed same feature with that of primary rhabdomyosarcoma.

신경 향상 기술 관련 신경윤리 연구를 위한 시나리오 개발 -뇌심부자극술(DBS)을 통한 기억 향상 기술을 중심으로-

류영준,엄주희 동국대학교 동서사상연구소 2022 철학·사상·문화 Vol.- No.39

This study contains the process of deriving specific technical ethics scenarios for neuroethics research. It based on the results of a human trial study of deep brain stimulation for the purpose of improving memory, which was recently conducted at Seoul National University Hospital and known through the media. This scenarios process was based on the results at Seoul National University hospital in 2020, presented by Professor Chun Kee Chung, a neurosurgeon and Soyeon Jeon a neuroscientist at the Department of Brain and Cognitive Science at Seoul National University. Based on this study, the scenario derivation method was divided into two stages. First of all, it was checked whether the basis for the preparation of the technical ethics scenario was appropriate in the six criteria. Criteria are 1) researcher's credibility and the existence of scientific basis, 2) unethical or illegality of research procedures, 3) commercial research purpose, 4) scientific specificity, 5) potential for development 6) Possibility of social impact. The “Deep Brain Stimulation Technical Ethics Scenario for the purpose of improving memory for test takers” thus derived is expected to be a necessary basis for neuroethicists and humanities scholars who wish to discuss humanities. 본 연구는 최근 서울대학교 병원에서 시행되고 언론을 통해 알려진 기억력 향상을 목적으로 한 뇌심부자극술의 인간대상 연구 결과를 바탕으로 신경윤리 연구를 위한 구체적 기술윤리 시나리오의 도출 과정을 고찰한 것이다. 2020년 서울대학교 병원 신경외과 의사인 정천기 교수팀은 10명의 난치성 간질 환자를 대상으로 기억을 담당하는 해마에 직접 전기 자극을 시행하여 연구결과를 발표하였다. 이 연구를 바탕으로 기술윤리 시나리오 도출하기 위하여 세 단계의 분석을 수행하였다. 연구자와 연구과정에서의 윤리성과 사회적 영향을 기준으로 6가지의 준거를 설정하고 해당 사례가 기술윤리 시나리오 작성에 적합한 사례인지 검토한 후, 구체적인 시나리오를 구성하였다. 1) 연구자의 신뢰도와 과학적 근거 유무, 2) 연구 절차의 비윤리성 또는 불법성, 3) 상업적 연구목적을 고려하여 연구결과 공표의 윤리성, 4) 과학적 구체성, 5) 해당 연구주제의 발전가능성 유무 6) 사회적 파장 가능성이 6가지 준거이다. 이를 통해 ‘수험생 대상 기억력 향상을 목적으로 한 뇌심부자극술 기술윤리 시나리오’가 도출되었다. 이 시나리오가 신경과학기술과 관련하여 인문‧사회과학적 논의를 하고자 하는 윤리학자와 인문·사회과학자들에게 필요한 구체적 논의의 틀을 제공할 수 있을 것으로 기대한다.

랑게르한스세포 조직구증 20예의 병리학적 특징과 Langerin (CD207)의 면역화학염색의 발현 특이성

류영준,정회선,신봉경,김애리,김한겸,김인선,이현주,이상호 대한병리학회 2009 Journal of Pathology and Translational Medicine Vol.43 No.2

Background : Langerhans cell histiocytosis (LCH) is a well-known neoplastic disorder of Langerhans cells which has characteristic findings, however, LCH has not been adequately studied in Korea. Methods : We analyzed the clinicopathologic features of 20 patients with LCH who were diagnosed between 1997 and 2006 at the Korea University Guro and Anam Hospitals. Results : The M:F ratio was 3:1 and the age ranged from 2-60 years (mean, 23.8 years [4 in 1st decade, 6 in 2nd decade, 2 in 3rd decade, 5 in 4th decade and 3≥40 years of age). The cases were classified as unifocal unisystemic in 13 patients, multifocal unisystemic in 4 patients, and multifocal multisystemic in 3 patients. The bone was the most commonly involved organ (14), followed by lymph node (5), lung (2), skin (2) and ureter (1). The Langerhans cells were immunohistochemically stained with Langerin, CD1a, S-100 protein, and CD68. Langerin and CD1a were specific for Langerhans cells. Conclusions : The distribution of the involved organs in patients with LCH was similar to the distribution in Western countries,but lymph node involvement was more frequent, whereas lung involvement was less common. Langerin is considered to be a specific marker for Langerhans cells. Background : Langerhans cell histiocytosis (LCH) is a well-known neoplastic disorder of Langerhans cells which has characteristic findings, however, LCH has not been adequately studied in Korea. Methods : We analyzed the clinicopathologic features of 20 patients with LCH who were diagnosed between 1997 and 2006 at the Korea University Guro and Anam Hospitals. Results : The M:F ratio was 3:1 and the age ranged from 2-60 years (mean, 23.8 years [4 in 1st decade, 6 in 2nd decade, 2 in 3rd decade, 5 in 4th decade and 3≥40 years of age). The cases were classified as unifocal unisystemic in 13 patients, multifocal unisystemic in 4 patients, and multifocal multisystemic in 3 patients. The bone was the most commonly involved organ (14), followed by lymph node (5), lung (2), skin (2) and ureter (1). The Langerhans cells were immunohistochemically stained with Langerin, CD1a, S-100 protein, and CD68. Langerin and CD1a were specific for Langerhans cells. Conclusions : The distribution of the involved organs in patients with LCH was similar to the distribution in Western countries,but lymph node involvement was more frequent, whereas lung involvement was less common. Langerin is considered to be a specific marker for Langerhans cells.