Genetic Transformation of Oriental Cymbidium by a Modified Agrobacterium Infection Method

Hye Joung Kim(김혜정),Sang Hyun Shin(신상현),Young Soo Chung(정영수),Jeong Sheop Shin(신정섭),Chun Ho Pak(박천호),Jong Suk Lee(이종석) 한국원예학회 2000 원예과학기술지 Vol.18 No.2

도시 내 육상 생물종 모니터링을 위한 환경DNA 리뷰 및 적용

김휘문 ( Kim Whee-moon ),김성열 ( Kim Seoung-yeal ),박일수 ( Park Il-su ),이현정 ( Lee Hyun-jung ),김경태 ( Kim Kyeong-tae ),김영 ( Kim Young ),김혜정 ( Kim Hye-joung ),곽민호 ( Kwak Min-ho ),임태양 ( Lim Tae-yang ),박찬 ( Park 한국환경복원기술학회(구 한국환경복원녹화기술학회) 2020 한국환경복원기술학회지 Vol.23 No.2

Scientific trust and quantification of traditional species investigation and results that have been used in ecology for decades has always been a problem and concern for ecologists. Global ecologists have proposed DNA-based species investigation studies to find answers to problems. In this study, we reviewed the global trend of research on environmental DNA(eDNA), which is a method for monitoring species by detecting DNA of organisms naturally mixed in environmental samples such as water, soil, and feces. The first eDNA research confirmed the possibility of species investigation at the molecular level, and commercialization of NGS(Next Generation Sequencing) and DNA metabarcoding elicits efficient and quantitative species investigation results, and eDNA research is increasing in the filed of ecology. In this study, mammals and birds were detected using MiMammal universal primers from 23 samples(3 natural reserves; 20 water bowls) out of 4 patches to verify eDNA for urban ecosystems in Suwon, and eDNA was verified by performing camera trapping and field survey. Most terrestrial species were detected through eDNA, and particularly, mice(Mus musculus), and Vinous-throated Parrotbill (Sinosuthora webbiana) were identified only with eDNA, It has been confirmed to be highly effective by investigating techniques for small and internal species. However, due to the lack of resolution of the primer, weasels(Mustela sibirica) and squirrels(Melanochromis auratus) were not detected, and it was confirmed that the traditional investigation method was effective only for a few species, such as Mogera robusta(Mogera robusta). Therefore, it is judged that the effects of species investigation can be maximized only when eDNA is combined with traditional field survey and Camera trapping to complement each other.

페로니켈슬래그 미분말 혼입 시멘트 페이스트의 양생조건에 따른 유동성 및 압축강도 특성

김영욱 ( Kim Young Uk ),김도빈 ( Kim Do Bin ),김혜정 ( Kim Hye Jeong ),정수빈 ( Jeong Su Bin ),김정현 ( Kim Joung Hyeon ),최세진 ( Choi Se Jin ) 한국구조물진단유지관리공학회 2017 한국구조물진단유지관리공학회 학술발표대회 논문집 Vol.21 No.2

The study investigated the fluidity and compressive strength properties of cement paste containing Ferro-Nickel slag powder by different curing conditions to estimate the applicability of Ferro-Nickel slag powder for cement replacement materials.

지속적 발열 환자에서 반복 혈액배양이 유효한 균 동정률에 영향을 미치는 지에 대한 근거기반간호 사례

김나현(Kim, Na Hyoun),최현임(Choi, Hyun Yim),이미순(Lee, Mi Soon),이미경(Lee, Mi Kyung),한다은(Han, Da Eun),김혜정(Kim, Hye Jung),최정순(Choi, Joung Soon),이미정(Lee, Mi Jung),박정옥(Park, Jeong Ok),서은정(Seo, Eun Jeoung) 한국근거기반간호학회 2020 근거와 간호 Vol.8 No.1

함황 채소 추출물의 항산화 효과에 관한 연구

김다미(Da Mi Kim),김혜정(Hye Joung Kim),조지은(Ji Eun Jo),윤미향(Mi Hyang Yoon),김경희(Kyung Hee Kim),육홍선(Hong Sun Yook) 한국생활과학회 2010 한국생활과학회 학술대회 Vol.2010 No.하계

무의미한 연명치료 중단 결정에서 의료의 한계

안용항 ( Yong Hang Ahn ),김혜정 ( Hye Joung Kim ) 한국의료윤리학회 2010 한국의료윤리학회지 Vol.13 No.1

On May 21, 2009, the Supreme Court of Korea ruled that patients have the right to terminate medically meaningless treatment upon confirmation that their terminal illness is indeed irreversible. The Supreme Court`s decision thus makes proof of the irreversibility of a patient`s condition an important process in legitimatizing a patient`s decision to terminate life-sustaining treatment. However, since medical practice rejects explanations in terms of "essences" and is affected by issues of subjectivity, we argue that physicians cannot give indisputable confirmation of the irreversibility of a patient`s terminal illness, as required by the Supreme Court`s ruling.

황 함유 채소 에탄올 추출물의 항산화 및 항균활성

김경희(Kyoung-Hee Kim),김혜정(Hye-Joung Kim),변명우(Myung-Woo Byun),육홍선(Hong-Sun Yook) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.5

본 연구는 황 함유 채소류 중 사용범위가 넓은 마늘, 무, 부추, 생강, 양파, 파를 80% 에탄올에 추출하여 항산화 및 항균효과에 대해 평가하였다. 추출 수율은 2.33~10.12%를 나타내었으며, 총 폴리페놀 함량은 생강이 233.63±4.59, 부추가 220.98±10.56 ㎎/g GAE로 높은 함량을 나타내었고, 양파, 파, 마늘, 무 순으로 69.07±1.42, 68.83±2.11, 19.41±0.40, 19.05±03.32 ㎎/g GAE의 함량을 나타내었다. DPPH radical 소거활성을 측정한 결과, 생강의 IC50 값이 1.57±0.15 ㎎/mL로 가장 높았으며, 이어 부추>양파>무>파>마늘의 순이었다. ABTS 라디칼 소거활성 및 FRAP value의 측정 결과 역시 생강 및 부추 추출물에서 높은 항산화활성을 나타내었으며 ABTS 라디칼 소거활성의 경우 파>양파>마늘>무, FRAP value의 경우 파>양파>무>마늘의 순으로 항산화 활성을 나타내었다. 항산화 활성 측정결과 전체적으로 생강에서 가장 높은 항산화활성을 나타내었다. 항균활성 측정결과, 부추의 경우 실험에 사용된 7가지 균주에 대해 모두 항균활성을 가지고 있는 것으로 나타났으며, 마늘의 경우(5 ㎎/disc), B. cereus(22.3 ㎜) 및 E. coli(24.3 ㎜)에 대해 높은 항균활성을 나타내었다. 양파와 파는 10 ㎎/disc 농도에서 E. coli(12.7 및 10.3 ㎜)와 B. cereus(12.0 및 12.5 ㎜) 균주에 대해서만 항균력을 나타내었고, L. plantarum 균주(10 ㎎/disc)에 대해서는 마늘(18.0 ㎜)과 부추(10.4 ㎜)가 항균활성을 나타내었다. 본 연구결과, 다양한 한국음식에 광범위하게 이용되는 황 함유채소류 중 생강 및 부추는 항산화 활성이, 부추 및 마늘은 항균력이 높아 특히 부추의 경우 식품 첨가물 및 식품 보존제로서도 활용될 수 있을 것으로 사료된다. This study investigated the antioxidant activities, and antimicrobial activity in vitro of an 80% ethanol extract from garlic, daikon, leek, ginger, onion, and green onion, which are widely-used ingredients in Korean food that contain sulfur. The total polyphenol content in ginger and leek extracts showed a high value (233.63±4.59 and 220.98±10.56 ㎎/g GAE) and onions, leeks, garlic, and daikon followed by with 69.07±1.42, 68.83±2.11, 19.41±0.40, 19.05±03.32 ㎎/g GAE, respectively. DPPH radical scavenging activity was highest with ginger extracts (1.57±0.15 ㎎/mL as IC50) followed in order of decreasing activity by leeks, onions, daikon, green onions, and garlic. The results of ABTS radical scavenging activity and FRAP value showed higher antioxidant activity in extracts from ginger and leek. The order of vegetables with most to least prevalent ABTS radical scavenging activity was green onions, onions, garlic, and finally daikon. From greatest to least FRAP value, the relevant vegetables were green onions, onions, daikon, and garlic (p<0.05). Ginger extracts showed promise against seven strains of microbes: Bacillus cereus, Bacillus subtillis, Staphylococcus aureus, Lactobacillus plantarum, Escherichia coli, Salmonella enterica, and Pseudomonas aeruginosa. Garlic extracts (5 ㎎/disc) showed strong antimicrobial activity against B. cereus (22.3 ㎜) and E. coli (24.3 ㎜). Extracts of both onion and green onion showed antimicrobial activity against only E. coli (12.7 and 10.3 ㎜) and B. cereus (12.0 and 12.5 ㎜) at 10 ㎎/disc, and the inhibition zone diameter from extracts of garlic and leeks were 18.0 ㎜ and 10.4 ㎜ vs. L. plantarum at 10 mg/disc. This study showed positive antioxidant activities for ginger and leeks, and positive antimicrobial activities for leeks and garlic. These sulfur-containing vegetables are widely used in Korean food. Leeks especially could serve as a functional food preservative.

말초혈액림프구의 Rosette 형성 및 세포독성에 미치는 중금속의 영향

김혜정,이정호 圓光大學校 醫科學硏究所 1998 圓光醫科學 Vol.14 No.2

Background: The hazards to heavy metals are keeping increase and mercury and cadmium are representative metals. The previous reports of the effects of these metals on immune responses do not come to an agreement. This study is designed to investigate the indirect effects of these metals on immune response employing the rossete forming rate(RFR) of bovine periphral blood lymphocytes. (PBL) to sheep red blood cells(SRBC) and their cytotoxicity to PBL. Method: Mercury or cadmium treated PBL were divided into two groups, respectively. One is treated at 37℃ with various concentration of HgCl_2(10^-7∼10^-4) or CdCl_2 for 90 min before mixed with SRBC, and then kept overnight at 4^0C(short- time treated group). The other was kept overnight at 4^0C with various concentration of these metals after mixed with SRBC(longtime, treated group). On the other hand, the effects of these metals on the cytotoxicity to PBL were evaluated by trypan blue exclusion method according to the same incubation procedure that described above. Results: The E-rossete forming rate and E_AET-rossete forming rate of mecury 90 min treated group were increased significantly (p<0.01) than those of control in range of 10^-6M and 10^-6∼10^-5M, respectively, but all of the 10^-4M treated PBL could hardly form rossete, especially in E-rossete. The E-rossete forming rate of mercury 14-16 hr treated group was increased than that of control group in range of 10^-7∼10^-6M, but there was no significance and E_AET-rossete forming rate was increased more than that of control in range of 10^-7∼10^-5(10^-7∼10^-6M, p<0.01). On the other hand, almost all of the 10^-4M treated PBL couldn't form rossete. The RFR of cadmium 90 min treated group was decreased in the proportion to the concentration of cadmium but E-rossete forming rate of 10^-5M and E_AET-rossete forming rate of 10^-6M were increased significantly(p<0.01) than those of controls. The E-rossete forming rate of cadmium 14-16 hr treated group was similiar to that of control but E_AET-rossete forming rate was increased in proportion to the concentration of cadmium in range of 10^-6∼10^-4M, but there was no significance. The cytotoxicity of mercury and cadmium was increased dose dependently but it was decreased in 14-16 hr treated group than that of 90 min treated group. Conclusion: These results suggest that mercury and cadmium modified immune response by changing the activity of SRBC receptors on the cell surface. On the other hand, cell dead rate induced by these heavy metals in 14-16 hr treated group was decreased than that of 90 min treated group, it suggests that damaged cells were recovered by adaption to these heavy metals and excretion of debris through intracellular organells.

NOD/SCID 마우스 모델을 이용한 인간 제대혈 혼합이식에서의 생착 양상 분석

정양조,김동욱,조빈,강영주,박보배,김혜정,김태규,오일환 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.2

연구배경: 제대혈은 골수에 비해 많은 장점을 가지고 있음에도, 이식 후 성적에 있어 총세포수에 의해 좌우되며, 한 개의 태반으로부터 얻을 수 있는 총세포수의 한계가 있어 주로 소아에 국한되고 있다. 따라서 제대혈 응용의 확대를 위해 생착량을 늘이기 위한 방법으로 다중공여자에 의한 제대혈 혼합이식을 늘일 수 있는지 연구하기 위하여, 본 연구에서는 주조직적합성 형별로 조합된 제대혈을 NOD/ SCID 생쥐에 이식하는 방법으로, 혼합생착의 가능성을 연구하였다. 방법: 조합된 2개 공여자로부터 얻어진 제대혈들을 단일이식 및 혼합이식하여, 혼합이식된 숙주에 생착된 세포를 PCR-SSOP 방법을 사용하여 공여자 별로 추적하였다. 또한 각 공여자세포의 상대적 생착비율을 대조군인 단일제대혈 이식에서 얻어진 생착량과 비교하는 정량적 비교도 함께 하였다. 결과: 총단핵구를 혼합이식한 실험군에서는 주조직적합성의 일치도에 관계없이 한쪽공여자의 세포가 다른쪽에 비해 우세한 편향생착을 보였다. 그러나 임파구를 비롯한 Lineage 양성세포를 제거한 실험군에서는 주조직적합성의 불일치에도 불구하고 뚜렸한 생착의 공존이 관찰되었다. 결론: 본 연구는 제대혈의 생착량을 증가를 목적으로 총단핵구를 혼합이식할 경우 한쪽공여자의 세포에 의해 편중되며, 이것을 이식 후 생착과정에서의 두 종류의 세포의 경쟁에 의해 초래된다는 것을 보이고 있다. 또한 이러한 경쟁은 제대혈에 포함되어 있던 임파구들에 의한 것이며, 생착공존을 위하여는 공여자간 주조직적합성의 일치도 보다, 임파구의 제거가 더 중요하다는 것을 보여주고 있다. Background: Although umbilical cord blood (UCB) has been an attractive alternatives for bone marrow cells, application is limited to pediatric case due to limitation in total number of nucleated cells, that is a single most important factor for outcomes of UCB transplantation. Therefore, increasing overall engraftment by mixed transplantation of UCB derived from multiple donor should comprise one strategy to circumvent the posed limitation of UCB transplantation. In order to investigate the feasibility of establishing co-engraftment by multi-donor UCB cells, we carried out a xenotransaplantation study using NOD/SCID mice for systemic analysis of results on the input-based control of single unit transplantation. Methods: UCB units with various extent of HLA-matchings were co-transplanted into NOD/SCID mice along with single unit transplantation control. The relative contributions of engraftment by cells from each donor-derived were analyzed by HLA polymorphism using PCR-SSOP. Results: In all HLA-based mixed transplantation of total nucleated UCB cells, engraftment of one donor predominated over the other despite that equivalent amount of engraftments were achieved by single donor transplantation. When lineage depleted UCB units were co-transplanted, significant degree of co-engraftments were observed regardless of HLA disparity. Conclusion: Our result show that one donor cells dominate over the other in mixed UCB transplantation and that it is due to competition between donor cells during post-transplantation process. Our results suggest that immune cells contained in UCB unit mediate such competitioin and that 6 locus HLA matching would not be sufficient to prevent the competition.