GM 대두 분석용 DNA 기준물질의 안정성 검증

김형하,이수경,김우정,서정근 한국환경분석학회 2013 제1회 한국분석기술단체 총 연합 합동 심포지엄 Vol.0 No.-

GM 옥수수(MON810) 분석용 DNA 기준물질의 장기안정성 검증

김형하,이수경,김우정,서정근 한국환경분석학회 2013 제1회 한국분석기술단체 총 연합 합동 심포지엄 Vol.0 No.-

물오리나무 ( Alnus hirsuta ) 의 칼루스 유도 및 자엽유래 칼루스로부터의 원형질체 배양

김형하(Hyong Ha Kim),안정선(Chung Sun An) 한국식물학회 1990 Journal of Plant Biology Vol.33 No.4

Callus-inducing ability of Alnus hirsuta was examined by culturing various tissues (leaf, hypocotyl, cotyledon and seed) on NT (Nagata & Takebe) medium, supplemented with 2.5 μM 2,4-D. Leaf-orginated callus was cultured on media varying in auxin (IBA and NAA) and cytokinin (BAP) concentrations to examine the effects of auxin and cytokinin on callus growth. Maximum growth was obtained at 10μM IBA + 10μM BAP and 10μM NAA without cytokinin. Cell suspensions established from cotyledon-originated callus yielded viable protoplasts after incubation for 16-18 hours in an enzyme mixture (1% (w/v) Onozuka R-10, 0.5% (w/v) Macerozyme, CPW salts and 13% (w/v) mannitol, pH 5.8). Protoplasts were cultured on NT medium, supplemented with glucose, hormones and coconut milk. After 6 weeks of culture, protoplasts sustained cell divisions to form microcallus, which showed various colors from red to white.

A New Analytical Method to Determine the Purity of Synthetic Fluorophores using Single Molecule Detection Technique

송남웅,김형하,박태숙,윤민중 한국광과학회 2005 Journal of Photosciences Vol.12 No.2

Infrared Multiphoton Dissociation of CHCI2F: Reaction Mechanisms and Product Ratio Dependence on Pressure and Laser Pulse Energy

송남웅,이원철,김형하 한국광과학회 2005 Journal of Photosciences Vol.12 No.2

Structural Identification of a Non-Glycosylated Variant at Ser126 for O-Glycosylation Site from EPO BRP, Human Recombinant Erythropoietin by LC/MS Analysis

변재희,임유리,김형하,서정근 한국분자세포생물학회 2015 Molecules and cells Vol.38 No.6

A variant peak was detected in the analysis of RP-HPLC of rHu-EPO, which has about 7% relative content. Fractions of the main and the variant peaks were pooled separately and further analyzed to identify the molecular structure of the variant peak. Total mass analysis for each peak fraction using ESI-TOF MS shows differences in molecular mass. The fraction of the main peak tends to result in higher molecular masses than the fraction of the variant. The detected masses for the variant are about 600-1000 Da smaller than those for the main peak. Peptide mapping analysis for each peak fraction using Asp-N and Glu-C shows differences in O-glycopeptide profiles at Ser126. The O-glycopeptides were not detected in the fraction of the variant. It is concluded that the variant peak is non-Oglycosylated rHu-EPO and the main peak is fully Oglycosylated rHu-EPO at Ser126.

Development of Aloe Fermentation Products and Improvements of Gastrointestinal Function in vitro

조주현,백순옥,김충식,김형하,정은주,김현경,김봉균 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.1

Three aloe fermentation products from Ganoderma lucidum (AG), Hericium erinaceum (AH), and Phellinus linteus (AP) were obtained by fermenting mushroom mycelia using aloe as a substrate. When AG, AH, and AP were added to sterilized aloe and fermented for 5 days, the color of the aloe fermentation product changed from pink to beige, which is aloe’s natural color, through the fermentation time. The pH of the aloe fermentation products ranged 4.32-4.36 shortly after inoculation and then 4.62-4.68during the 5 days of fermentation. pH increased by 7%during the total fermentation time. The solid content had increased 1.28-1.40 times. The contents of aloin A and B increased with fermentation time. β-Glucan content decreased with fermentation time. The urease inhibition activity (%)were remarkable in AG-4 96.70, AH-4 92.30, and AP-466.40%, indicating these products had growth inhibition effects against Helicobacter pylori. Moreover, AG and AH were most effective as anti-H. pylori treatments.

정서장애 대학생을 대상으로 한 단일화된 범진단적 치료의 효과

조용래,남수아,서우정,김형하 한국인지행동치료학회 2023 인지행동치료 Vol.23 No.1

본 연구는 Barlow 등(2011)에 의해 개발된 정서장애에 대한 단일화된 범진단적 치료 프로토콜(UP)의 5가지 핵심모듈과 3가지 추가 모듈에 기반한 총 8회기의 심리학적 치료가 여러 종류의 불안장애와 우울장애로 진단된 국내참가자들에게 효과적인지를 검증하는 데 목적이 있다. 연구 대상은 DSM-5의 사회불안장애, 주요우울장애, 지속성우울장애, 범불안장애 및 기타 불안 관련 장애 중에서 적어도 1가지 이상 충족시킨 대학생들이며, UP 개인치료를 제공받은 참가자 18명과 대기통제조건에 속한 참가자 15명이 8주 간격의 사전 및 사후 평가와 3개월 추후 평가를 모두 완성하였다. UP조건에서 사회불안증상, 범불안증상, 우울증상, 임상적 불안증상, 부적 정서 및 주 진단의 임상적 심각도 평정 등의 측정치들이 사전과 사후 간에, 사전과 추후 간에 유의하게 감소하였다. 이 조건에서공황장애 심각도와 일상적 기능의 손상 수준은 사전에 비해 추후에 큰 수준 및 중간에서 큰 수준 사이의 효과크기를 각각 보였다. 이와 대조적으로, 대기통제조건은 대부분의 측정치들에서 그러한 변화를 거의 보이지 않거나 작은 변화를 보였다. 임상적 유의성 검증 결과, UP조건은 대기통제조건에 비해 주 진단의 기준을 더 이상 충족시키지 않은 참가자의 비율, 치료 반응자 비율, 그리고 높은 최종 상태 기능자 비율 등이 유의하게 더 높았다. 결론적으로, 이러한 결과들은 총 8회기의 UP가 여러 종류의 불안장애와 우울장애로 진단된 국내 대학생 내담자들에게 효과적임을 시사하며, 국내에서 정서장애에 대한 범진단적 치료의 임상적 적용 가능성을 지지한다.

Identification of Disease Specific Protein Interactions between the Gastric Cancer Causing Pathogen, H. pylori, and Human Hosts using Protein Network Modeling and Gene Chip Analysis

김완규,김규완,이은정,Edward M. Marcotte,김형하,서정근 한국바이오칩학회 2007 BioChip Journal Vol.1 No.3

Proteins are traditionally known as the building blocks or functional units that make up the cellular physiology of living organisms. In the post-genomic view of a protein, however, it can function as an element within a protein network and its role can then be evaluated by protein-protein interaction analysis. The role of proteins within such a network can be defined by their cellular function within the functional modules of the network as well as their individual activity. In this study, we used a proteinprotein interaction modeling system to identify the functional modules and proteins involved in the pathogenic interaction between the gastric pathogen, <I>H. pylori</I>, and humans. We analyzed 1,590 <I>H. pylori</I> proteins against 10,257 human entries expressed in human gastric tissues and identified 4,349 potential protein-protein interactions between 159 <I>H. pylori</I> proteins and 108 human proteins. We then investigated the association of gastric cancer with the 108 human proteins found to have an interaction with the <I>H. pylori</I> proteins using a GeneChip database that we generated. Among the 108 human proteins, 93 (86%) were shown to be associated with gastric cancer, 91 of which were up-regulated and 2 of which were down-regulated by at least 4 fold in gastric cancer tissues. Additionally, 32 of the proteins were found to be gastric cancer-specific, whereas the remaining proteins were found to be associated with several other forms of cancer. Taken together, these results suggest that protein network modeling in conjunction with GeneChip technology can be a useful tool for the analysis of the complex relationship between human pathogens and their hosts.

가시오가피 버섯균사체 발효물의 기능성 식품 소재 개발

조주현(Ju-Hyun Cho),최구희(Goo-Hee Choi),박인재(In-Jae Park),백순옥(Soon-Ok Baik),김형하(Hyung-Ha Kim),김충식(Choong-Sik Kim) 한국식품영양과학회 2014 한국식품영양과학회지 Vol.43 No.3

가시오가피(Acanthopanax senticosus)의 잎과 줄기를 천연물배지로 발효미생물인 영지버섯, 노루궁뎅이버섯 및 상황버섯균사체를 각각 최적조건으로 발효하여 3종의 가시오가피-버섯발효물인 가시오가피 영지버섯균사체 발효물, 가시오가피 노루궁뎅이버섯균사체 발효물, 가시오가피 상황버섯균사체 발효물을 얻었으며 이를 건강기능식품 소재로서 이용하고자 하였다. 우선적으로 배양용 시험관과 실험실용 배양병을 사용하여 버섯균사체별 가시오가피의 천연배지로서 최적혼합비율을 검토한 결과 영지버섯균사체, 노루궁뎅이버섯균사체 및 상황버섯균사체의 가시오가피 줄기와 잎의 혼합비율을 각각 80:20, 50:50, 50:50으로 확립하였으며, 이 조건을 가지고 산업용 배양포트(2,000 mL)에서의 최적배양시간을 검토한 결과 영지버섯균사체 14일, 노루궁뎅이버섯균사체 40일, 상황버섯균사체 30일로 확립하였다. 상기와 같이 최적화된 조건으로 배양된 3종의 가시오가피버섯균사체 발효물들을 물과 70% 에탄올로 추출, 여과, 농축, 동결건조를 수행하여 가시오가피 버섯균사체 발효물 추출분말인 열수추출물(FM-5111, FM-5121, FM-5131)과 70% 에탄올추출물(FM-5112, FM-5122, FM-5132)을 얻었다. 이 가시오가피 버섯균사체 발효물 추출분말의 eleutheroside B와 eleutheroside E를 분석한 결과, eleutheroside B는 FM-5111 0.050±0.003 mg/g, FM-5121 0.067±0.001 mg/g, FM-5131 0.026±0.001 mg/g, FM-5112 0.038±0.002 mg/g, FM-5122 0.053±0.005 mg/g, FM-5132 0.025±0.001 mg/g의 함량을 나타내었고, eleutheroside E는 FM-5111 0.052±0.014 mg/g, FM-5121 0.047±0.003 mg/g, FM-5131 0.078±0.003 mg/g, FM-5112 0.045±0.002 mg/g, FM-5122 0.040±0.002 mg/g, FM-5132 0.080±0.011 mg/g의 함량을 보였으며, β-glucan의 경우 FM-5111 4.47±0.14%, FM-5121 3.41±0.15%, FM-5131 4.15±0.06%, FM-5112 5.18±0.36%, FM-5122 3.74±0.40%, FM-5132 4.37±0.32%의 함량이 측정되었다. Three mushroom mycelia, Ganoderma lucidum, Hericium erinaceum, and Phellinus linteus, were separately diluted with the natural culture media Acanthopanax senticosus. Solid-state fermentation was used to produce three different A. senticosus-fermented mushroom mycelium groups: G. lucidum mycelia, H. erinaceum mycelia, and P. linteus mycelia. The resulting mycelia were analyzed to assess their efficacies as health functional foods. Optimized fermentation conditions were determined by considering the density and growth speed of mycelia in each A. senticosus- fermented mushroom mycelium group. The cultured mushroom mycelia under the optimized conditions were extracted using water and 70% ethanol. Extraction was followed by filtration, concentration and freeze-drying to produce extract powder of A. senticosus-fermented mushroom mycelia: Water extracts (FM-5111, FM-5121, and FM-5131) and 70% ethanol extracts (FM-5112, FM-5122, and FM-5132). Analysis of extract powder of A. senticosus-fermented mushroom mycelia was performed using the maker compounds eleutheroside B and eleutheroside E. Analysis of β-glucan contents was performed by enzymatic procedures.