Effect of ER signal peptides (KDEL) on expression and function of monoclonal antibody in plant

Da-Young Park,Eun-Yi Oh,Zhe Lu,Yangkang So,Mi-Hyun Ahn,Mira Song,Kyung-Jin Lee,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current animal expression systems. In this study, the expression level of mAbP SO57 between with and without Lys-Asp-Glu-Leu (KDEL) was compared in transgenic plant. PCR and PT-PCR analyse showed stable gene insertion transcription of heavy and light chain genes of mAbP SO57 with or without KDEL in plant, respectively. We validated expression of mAbP SO57 by western blot. Western blot showed the significantly higher expression level of mAbP SO57 with KDEL compared to without KDEL. Flow cytometry (FACS) analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb (mAbM) evidenced similar binding activity to the FcγRI receptor (CD64). High performance liquid chromatography (HPLC) analysed, glycosylation patterns of mAbP SO57 with or without KDEL. The mAbP SO57 with KDEL had glycan profile with both oligomannose type (47.6%) and golgi type (52.4%), while the mAbP SO57 without KDEL had only golgi type (100%) glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest the potential of mAbP SO57 for rabies immunotherapy, regardless of plant specific glycan structures.

Effect of ER signal peptides (KDEL) on expression and function of anti-virus monoclonal antibody in plant

Da-Young Park,Yangkang So,Kyung-Jin Lee,Zhe Lu,Eun-Yi Oh,Kwang-Wok Min,Seul-Ki Lee,Jung-Hwan Lee,Mi-Hyun Ahn,Mira Song,Doo-Byoung Oh,Youngkwan Kim,Kisung Ko 한국당과학회 2009 한국당과학회 학술대회 Vol.2009 No.1

Plant genetic engineering has led to the production of plant-derived mAb (mAbP), which provides a safe and economically feasible alternative to the current antibody expression systems. In this study, the expression levels of mAbP SO57 with or without ER-signal peptides (Lys-Asp-Gly-Leu;KDEL) in transgenic tobacco plants were analysed in transgenic plant. PCR and Reverse Transcription-PCR analyses showed existence of heavy and light chain genes of mAb with or without KEDL and their transcription in plant, respectively. Western blot showed that the expression levels of mAbP SO57 with KDEL were significantly higher than that without KDEL. Flow cytometry analysis showed that the Fc domains of both purified mAbP and mammalian-derived mAb have similar binding activity to the FcγRI receptor. High-performance liquid chromatography analysis showed that the mAbP SO57 with KDEL had glycan profile with both oligomannose and golgi type, whereas the mAbP SO57 without KDEL had only golgi type glycans. Neutralizing analysis with rabies virus CVS-11 showed the similar neutralizing activity between mAbP SO57 with and without KDEL. These results suggest that the potential of mAbP SO57 for rabies immunotherapy is regardless of plant specific glycan structures.

Cross-talk between STAT6 and Ras/MAPK Pathway for the IL-4-mediated T Cell Survival

So, Eui-Young,Jang, Ji-Young,Lee, Choong-Eun Korean Society for Biochemistry and Molecular Biol 2001 Journal of biochemistry and molecular biology Vol.34 No.6

As a prototypic Thl vs Th2 cytokine, IFN-$\gamma$ and IL-4 activate distinct STAT proteins, STAT1 and STATE, respectively. In cytokine-producing Jurkat T cells, IL-4 is effectively rescued from cell death that is induced by dexamethasone, but IFN-$\gamma$ failed to do so. Since the Ras/MAPK pathway is known to play an important role in cytokine-induced cell survival, we investigated the mechanism of T cell survival through the analysis of functional cross-talk between Ras/MAPK and distinct STAT proteins that are activated by IL-4 and IFN-$\gamma$. Although IL-4 and IFN-$\gamma$ each induced the activation of STATE and STATI. in Jurkat T cells, respectively, only IL-4 was capable of inducing MAPK. Along with tyrosine kinase inhibitors, MEK/MAPK inhibitors also caused a significant suppression of the IL-4-induced STATE activity. This suggests a positive regulation of STATE by MAPK during IL-4 signal transduction. Furthermore, transfection studies with dominant active (da) vs dominant negative (dn) Ras revealed that daRas, but not dnRas, selectively up-regulated the expression and activity of STATE with a concomitant increase in MAPK activity. These results, therefore, suggest that there is a functional cross-talk between the Ras/MAPK and Jak/STAT6 pathways, which may have a role in the IL-4-induced T cell survival.

Cross - talk between STAT6 and Ras / MAPK Pathway for the IL - 4 - mediated T Cell Survival

Lee, Choong Eun,So, Eui Young,Jang, Ji Young 생화학분자생물학회 1998 BMB Reports Vol.34 No.6

As a prototypic Thl vs Th2 cytokine, IFN-γ and IL-4 activate distinct STAT proteins, STAT1 and STATE, respectively. In cytokine-producing Jurkat T cells, IL-4 is effectively rescued from cell death that is induced by dexamethasone, but IFN-γ failed to do so. Since the Ras/ MAPK pathway is known to play an important role in cytokine-induced cell survival, we investigated the mechanism of T cell survival through the analysis of functional cross-talk between Ras/MAPK and distinct STAT proteins that are activated by IL-4 and IFN-γ. Although 1L-4 and IFN-γ each induced the activation of STATE and STATI. in Jurkat T cells, respectively, only IL-4 was capable of inducing MAPK. Along with tyrosine kinase inhibitors, MEK/MAPK inhibitors also caused a significant suppression of the IL-4-induced STATE activity. This suggests a positive regulation of STATE by MAPK during IL-4 signal transduction. Furthermore, transfection studies with dominant active (da) vs dominant negative (dn) Ras revealed that daRas, but not dnRas, selectively up-regulated the expression and activity of STATE with a concomitant increase in MAPK activity. These results, therefore, suggest that there is a functional cross-talk between the Ras/MAPK and Jak/STAT6 pathways, which may have a role in the IL-4-induced T cell survival.

남자대학생의 흡연태도와 행동에 관한 연구

강경진,김정미,민혜영,안소진,오경숙,은경희,이현,이희선,홍주미,김수지 이화여자대학교 간호과학대학 2003 이화간호학회지 Vol.- No.37

There have been so many researches about problems of cigarettes and relation of smoking and heath since 1950's and generally known that smoking is one of the worst factor of increasing disease and mortality. No matter what such many proofs about various problems of smoking have been researched, smoking rate of current male collage students has not been decreased. Besides, most of the researches about cigarette use and its addiction have been focused on the adolescent or adult women smokers or adult male, not male collage student. Therefore, the subject of this study is about smoking attitude and smoking behavior of male collage students by general features. Smoking attitude was analyzed by modifying 'Guidelines for the content of tobacco smoking surveys of the general population'(WHO, 1982). Two hundred of male collage students participated to the distributed questionnaire for one week in May. The smoking attitude was categorized into duration of smoking and number of cigarette per day(CPD), and nicotine dependency, investigated by Fagerstrom Tolerance Questionnaire(FTQ) (Fagerstorm & Schneider, 1989). All statistics were performed by procedure of SAS according to number, percentage, mean, standard deviation, and ANOVA. The results are as follows: 1. Smoking attitude level was mean 25.73±5.15 marks, ranging from 13 to 39 marks of the total 40 marks. 2. Duration of cigarettes, number of cigarette per day, and nicotine dependency were mean 5.14±2.84 years, 12.88±6.85 pieces, and 4.78±1.69, respectively. 3. Smoking attitude showed significance according to general features such as marital state and allowance, but the significance according to marital state is not valuable because the numerical difference is so big between married one and single that they cannot be compared with each other. 4. Duration of smoking was significant according to general features such as age and allowance. 5. Smoking amount showed significance the factors of age only. 6. ll1ere was no significance in nicotine dependency according to general features. As a result, smoking attitude of male collage student in this study shows moderate level. Smoking attitude and duration is significant according to allowance, and smoking amount is significant according to age. Nicotine dependency according to general features is not significant but comparing with the preceding study about adult male, male collage students in this study are respectively more nicotine-dependent than adult male smokers. In conclusion, the right allowance usage strategies and effective heath education focused on the young are needed. In addition, helpful programs and information spreading strategies are necessary for establishing higher level of smoking attitude. At last, systemic regulations in daily living for ""anti-nico-dependent"" and perspective reforming of male university student are needed for reducing nicotine dependency.

회전원통수조 실험에서 회전속도에 따른 표류각속도

소선섭,강민구,김영은,조미순 公州大學校 基礎科學硏究所 2003 自然科學硏究 Vol.10 No.-

본 논문은 Kazuo Ukaji와 Katsumi Tamaki의 논문 중 회전속도(回轉速度)와 표류각속도(漂流角速度)의 관계를 기초로 하였다. 그들에 따르면, 회전삼중수조 실험값이 수치모델값보다 열손실에 의해 약 10% 정도 작고, Ω(회전속도)값이 증가하면 ω(표류각속도)는 점차적으로 감소함을 알 수 있었다. 본 논문에서는 그것을 실험으로써 증명하고자 하였다. 온도차(∆T)를 일정하게 유지하고 회전속도를 증가시키면서 파를 관찰하였다. 그 결과, 회전속도(回轉速度)를 크게 할수록 파의 표류각속도는 점차 감소하는 경향을 나타냈다. 또 회전속도에 따른 표류각속도의 기울기는 온도차가 작은 경우에 기울기가 더 높게 나타났다. This essay was based on relation of the drift angular speed and angular velocity in the essay of Kazuo Ukaji and Katsumi Tamaki. According to them, because of heat loss, rotating fluid annulus experimental value is lower about 10 percent than numerical model value, we could know that ω is dwindling if Ω increases. In this essay, we observed to certify it as an experiment. We kept differences of temperature changelessly, and observed form of waves as increasing turning number of times. By the result, when we changed greatly volutions per minute, observed hat the drift angular speed of wave is dwindling. Also, by the result, we observed tilt of a drift angular speed by angular velocity, slant in case difference of temperature is low.

국내 체중 감량 약물 사용 현황: 자발적으로 온라인에 게시한 처방적 분석

이소영,이지은,김미리,정은아,조은 건강보험심사평가원 2023 HIRA RESEARCH Vol.3 No.2

Background: Multiple medications have been used for weight loss (WL); however, research on the regimens of WL treatment in Korea have been limited. This study aimed to characterize the medications prescribed for WL in real-world settings. Methods: The study sample comprised WL prescriptions (Rxs) shared via a portal site for 1 year (from June 2022 to May 2023). WL drugs were classified into four categories: (1) on-label WL drugs; (2) off-label WL drugs; (3) medication-induced symptom management drugs; (4) dietary supplements and others. We calculated prescribing occurrence rates for each category and drugs. Further, we investigated the usage of off-label and psychotropic drug prescribing. Results: The study sample included 66 Rxs consisting of 63 active ingredients. The average number of medications per Rxs was seven (range, 1–13). Acetaminophen/caffeine/(pseudo)ephedrine (59.1%) and topiramate (54.5%) were the most commonly used medicines. The most frequently prescribed drug regimen involved a combination of on-label WL, off-label WL, and medication-induced symptom management drugs. Conclusion: Psychotropic appetite suppressants have been prescribed with unapproved WL drugs or psychotropic anti-anxiety/hypnotics. Further research is needed to provide evidence on drug combinations to ensure the safety of WL treatments.

신선초의 생장에 대한 이온교환수지의 재배 효과

이소라, 오영제, 김은주, 오영민 忠北大學校 農業科學硏究所 2014 農業科學硏究 Vol.30 No.2

This study was conducted to evaluate effect of ion exchange resins on growth of angelica (Angelica keiskei), and additionally investigated growth response of angelica according to germanium concentration in rhizosphere using the ion exchange resins containing germanium. As a result, the addition of the ion exchange resins induced significant increase of fresh weight and dry weight at 55 days of transplanting, and plant height and leaf area were also showed the similar pattern. Stem diameter and number of petioles were significantly higher in AS-62 and AS-64 treatment than in control. Inorganic elements concentration of angelica plants substantially didn’t show significant differences between control and treatments, but nitrogen, phosphorus, and potassium concentration were higher in treatments than in control. These results showed that the growth of angelica appeared to be promoted due to increased absorption rate of these elements caused by treatment of the ion exchange resins. There were no significant differences between control and treatments in the concentration of calcium, iron and boron, except for magnesium concentration of shoots. Germanium was not detected in the plants. In conclusion, the addition of ion exchange resin containing essential elements to soil showed positive effect on angelica growth.

사군자탕이 L1210 세포를 이식한 마우스의 면역세포에 미치는 영향

殷載淳,金大根,柳東和,權鎭,徐龍勳,蘇俊魯,全焄,吳贊鎬 우석대학교 의약품개발연구소 1997 藥學硏究誌 Vol.2 No.-

The purpose of this research was to investigate effects of Sa-Kunja-Tang(SKT) on immune cells of L1210 cell-transplanted mice. The apoptosis and T lymphocytes subopoulation were tested using a flow cytometry, and the proliferation was tested using a MTT assay. Nitric oxide production from mouse peritoneal macrophage was tested using a Griess reagents, and the phagocytic activity of mouse peritoneal macrophage was tested using a lucigenin chemiluminescence. SKT suppressed apoptosis of T-lymphocytes induced by L1210 transplantation. SKT decreased nitric oxide production from mice peritoneal macrophages increased by L1210 transplantation, and the phagocytic activity decreased by L1210 transplantation. These results suggest that SKT suppresses T lymphocyte apoptosis and macrophage activity in L1210 transplanted mice.

뇌졸중환자를 돌보는 가족의 불쾌감과 스트레스

소희영,최은숙 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

The purpose of the study was to explore malaise and stress of CVA patient's family. The subjects of this study were consisted of 83 CVA patient's family caregivers, hospitalized at one University hospital and three general hospitals in Daejeon. The survey instruments used in the study were Rutter, Tizard & Whitmore's malaise inventory and Lee's stress inventory. The survey was conducted from July 16th to August 30th, 1992. The survey results were analyzed by using S.P.S.S. and summerized as follows ; 1. There was statistically significant difference in the caregiver's malaise according to their demographic characteristics such as age (F=4.08, P<.05), sex (t=-3.45, P<.001), family structure (t=1.93, P<.05), and relationship with patient (F=2.82, P<.05). 2. There was statistically significant difference in the caregiver's stress according to their demographic characteristics such as age (F=6.42, P<.01), educational level (F=6.42, P<.01), family structure (t=2.44, P<.01), monthly income (F=3.26, P<.05), relationship with patient (F=5.76, P<.001). 3. Mean of caregiver's malaise was 8.52, and stress was 45.56. 4. Malaise were negative correlation with the caregiver's educational level (γ=-.2068, P<05), and stress were negative correlation with age (γ=-.3651, P<.001), positive correlation with educational level (γ=.2722, P<.01) and monthly income (γ=.2653, P<.01). 5. In stepwise multiple regression analysis, the caregiver's monthly income, educational level and age accounted for 13.3% of perceived stress.