인간포배기 배아의 효과적인 유리화 동결법의 개발을 위한 연구

이상민,이주희,이상원,이승민,윤산현,임진호,박흠대,이성구,Lee, Sang-Min,Lee, Ju-Hee,Lee, Sang-Won,Lee, Seoung-Min,Yoon, San-Hyun,Lim, Jin-Ho,Park, Huem-Dai,Lee, Seong-Goo 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.3

Objective: The purpose of this study was to evaluate the survival rate of vitrified blastocyst according to the freezing vessels, equilibration time in cryoprotectant and artificial dehydration method. Methods: Human blastocysts were vitrified after loading onto the plastic straw, open-pulled straw (OPS), electron microscopy grid (EM grid) for 1.5 min or 3 min. They also were directly plunged into LN2 within 30sec. For artificial shrinkage of blastocysts, 36 gauge fine needle was pushed at the cellular junction of the trophectoderm into the blstocoele cavity until it shrank without damage of inner cell mass. Results: The survival rate of vitrified blastocysts on plastic straw, OPS, EM grid as freezing vessels were 26.7, 13.0 and 60.5%, respectively. The survival rate of EM grid was significantly higher than that of plastic straw and OPS (p<0.05). For 1.5 min equilibrium, the survival rates of early blastocyst (EB), middle blastocyst (MB) and late blastocyst (LB) were 64.4, 81.0, and 20.0% respectively. For 3 min equilibrium, the survival rates of EB, MB, and LB were 69.9, 50.0 and 57.5% respectively. The survival rates of EB and MB were significantly higher than that of LB in 1.5 min equilibrium group (p<0.05), however, the significance was not observed in 3 min equilibrium groups. In cytoplasmic shrinkage before vitrification, the survival rates of EB, MB and LB were 92.9, 100 and 75.9% respectively. The survival rate of MB was significantly higher than that of LB (p<0.05). The survival rates of vitrified blastocysts by artificial dehydration and slow-frozen blastocysts were not significantly different as 88.9 and 66.7%, respectively. Conclusion: This study showed that the vitrification of human blastocysts using EM grid and artificial dehydration is an effective method. Therefore, these methods would be an useful techniques for blastocyst cryopreservation.

Time-Related Changes in Detrusor Overactivity in Awake Rats with Spinal Cord Injury Observed by Simultaneous Registrations of Intravesical and Intraabdominal Pressures

Jin, Long-Hu,Shin, Hwa-Yoan,Yoon, Seung-Hwan,Seong, Do-Hwan,Park, Chang-Shin,Lee, Tack,Yoon, Sang-Min Korean Continence Society 2011 International Neurourology Journal Vol.15 No.1

<P><B>Purpose</B></P><P>To compare the physical characteristics of detrusor overactivity (DO) induced by intravesical infusion of saline in awake, sham rats and rats with chronic spinal cord injury (SCI), by simultaneous registrations of intravesical and intraabdominal pressures.</P><P><B>Methods</B></P><P>Male Sprague-Dawley rats, normal or with a spinal vascular clip at the level of Th9, were investigated cystometrically 1 and 4 weeks after SCI. Intravesical pressure (IVP) and intraabdominal pressure (IAP) were recorded simultaneously to evaluate true DO. During the filling phase, the event of IVP rises, defined as increments that exceeded 2 cmH<SUB>2</SUB>O from baseline, were determined as DO according to the absence of simultaneous changes in IAP.</P><P><B>Results</B></P><P>All SCI rats exhibited DO during the filling phase, which was not shown in sham rats. The frequency and pressure of DO had a tendency to decrease with time. The DO frequency of SCI rats after 4 weeks (0.9±0.2 min<SUP>-1</SUP>) was decreased compared with that after 1 week (2.1±0.4 min<SUP>-1</SUP>; P<0.05). The DO pressure of SCI rats after 4 weeks (8.4±1.9 cmH<SUB>2</SUB>O) was decreased compared with that after 1 week (11.6±2.9 cmH<SUB>2</SUB>O; P>0.05).</P><P><B>Conclusions</B></P><P>Cystometric studies in awake male SCI rats showed some significant changes in bladder function after SCI. All SCI rats exhibited DO during the filling phase, and showed different physical characteristics of DO over the course of time. The neurological basis of these time-related changes remains poorly understood, but may provide important prognostic information about long-term urological management in SCI patients.</P>

저전력 모드 Bluetooth 시스템을 이용한 파일 전송에 관한 성능 분선

윤현성,이민혁,변건식 동아대학교 정보기술연구소 2002 情報技術硏究所論文誌 Vol.9 No.2

Bluetooth is the name given to a new technology standard using short-range radio links, intended to replace the cable(s) connecting portable and/or fixed electronic devices. The standard defines a uniform structure for a wide range of devices to communicate with each other, with minimal user effort. Its key features are robustness, low complexity, low power and low cost. The standard is aimed at achieving global acceptance such that any Bluetooth device, anywhere in the world, can connect to other Bluetooth devices in its proximity , regardless of brand. Bluetooth enabled electronic devices connect and communicate wirelessly via short-range, ad hoc networks called piconets. Each unit can simultaneously communicate with up to seven other units per piconet. Moreover, each unit can simultaneously belong to several piconets. These piconets are established dynamically and automatically as Bluetooth devices enter and leave the radio proximity.

강진만 해역의 다모류 군집의 종조성 및 계절변동

윤성민,김춘철,서원일,황선영,이성훈,한경호,이우범 國立麗水大學校 環境問題硏究所 2004 環境硏究論文集 Vol.6 No.-

To detemine seasonal fluctuations in abundance and speices composition, polychaete samples were collected by Van Veen grab form March to November 2003 in Kangjin Bay. A total of 25,119.3 ind./㎡ polycaete were sampled and identified into 48 species. 25 families and 10 orders. Of the 10 orders. Phllodocida, Spionida, Eunicida and Sabellida accounted for approximately 79.9% of the polychaete fauna in this area. Phyllodocida, Nereidae, Nephtyidae ans Spionidae were dominant representing every moment 4 species. Of 48 species identified Neanthes japonica, Prionospio pinnate, Cirratulus cirratus and Palola siciliensis for 54.0% individuals collected. Season succession of dominant species was evident in study area: Neanthes japonica, Prionospio pinnata. Cirratulus cirratus. Nephtys oligobranchia in March, Neanthes japonica, Lumbrineris japonica. Cirratulus cirratus, Prionospio pinnata in May, Ncanthes japonica, Cirratulus cirratus, Lumbrineris nippnica. Lumbrineris japonica in August and Neanthes japonica, Prionospio pinnata, Laonice cirrata, Palola siciliensis in November, respectively. The diversity index was the highest value in March(H'=3.0059) and the lowest value in May(H'=2.7305). The eveness index was the highest in August(J=0.9184) and lowest in May(J=0.8524). The dominance index was the highest in May(D=0.3843) and the lowest in March(D=0.2275).

충남대학교 기초과학분야의 효율적 육성에 관한 연구

윤화중,진성일,윤민중,윤혜수,이영하,최철규,이천배,신대현 충남대학교 자연과학연구소 1986 忠南科學硏究誌 Vol.13 No.2

Chungnam National University (CNU) is located in Daeduk Science Town, a center of science and technology in Korea, and it is expected to play an important role in the development of science in Korea. With this in mind, the basic natural sciences at CNU should be more actively supported in both education and research. Accordingly analysis and comparison of the basic science program at major college in Korea as well as those in the USA and Europe has been performed. The current cooperative relationship between CNU and research institutes in the science town has also been analyzed. This study has resulted in propositions to make more efficient the upbringing of the basic sciences at CNU, by focusing on the following points ; a) to improve academic affairs, b) to make graduate studies substantial, c) to secure highly qualified faculty members, d) to increase cooperation in research between CNU and reserch institutes, e) to enlarge research laboratories and acquire more equipment and facilities.

하도급 중소기업의 R&D 활동 결정요인

윤성민 한국중소기업학회 2003 中小企業硏究 Vol.25 No.1

본 연구의 목적은 하도급 중소기업 R&D 활동의 다양한 동기를 설명탈 수 있는 이론적 가설들을 제시하고, 이 연구가설들을 통계적으로 검정하는 것이다. 본 연구에서 제시하는 이론적 연구가설들에는 크게 기업의 특성, 제품유형 및 판매방범, 자금조달 측면, 기술적 측면의 네 가지 범주에 속하는 결정요인들이 포함되어 있다. 본 연구에서는 중소기업청·중소기업 협동조합중앙회(1999)의 『중소기업실태조사보고』의 원자료에 수록된 자료를 이용하여, 1-검정과 10git 및 probit 모형의 추정치레 대한 검정은 통하여 연구가설들의 통계적 타당성을 검정하였다. 이 두 모형의 추정결과로부터 기업규모와 기술적 복합성의 두 요인이 하도급 중소기업 R&0 합동에 대한 가장 중요한 결정요인이며 그 외에 수급의존도, 수출비중, 기술교류도 주요 결정요인이라는 사설을 알 수 있었다. The purpose of this paper is to present theoretical hypotheses on the determinants of R&D activities of subcontracting small firms and to test those empirically. The hypotheses Presented in this paper are consisted of firm characteristics, product type and sales method, funds raising method, and technological factors. We tested those hypotheses with the method of t-test and test on estimates of legit and probit model. And we used the raw data of 'Small and Medium Business Survey' of Small and Medium Business Administration(SMBA) and Korea Federation of Small and Medium Business(KFSB). We found that firm size and technological complexity are the most important determinants of R&0 activities of subcontracting small firms, and that subcontracting dependence, export ratio and technological interchange are also important determinants.

有機農業의 實態와 發展에 관한 事例 硏究 : 忠南 洪城郡 洪東面 有機農家를 中心으로

尹英相,洪性贊,金成敏,具漢謨,申東一,金昌護,姜熙慶 公州大學校 資源科學硏究所 1997 資源科學硏究論文集 Vol.5 No.-

This study was carried out to improve the organic farming system about crop cultivation, compost production and application, plant diseases & pest control, weed control and marketing system of organic farming products to the case study in Chungnam Hongdong area. Agricultural technologies concerning pest & diseases control, weed control and compost production are not established due to lacks of systemized researches by the agricultural scientist, and agricultural machines profitable to this works had not developed yet. In cropping system, reasonable crop rotation had not conducted. The related cultivation of vegetables on the same ground for a long time cause a various plant disease easily. So crop rotation is a reasonable method about soil nutrient utility and soil disease control. It is a good method that introduce the barley and wheat in this field crop system. Those crop have a long root, so can use the other nutrient against the vegetable. In organic farming products, the safety and quality of products by the National Agricultural Products Inspection Center had not inspected except rice, lettus and apple. Inspection is needed for the development of organic farming and mutual confidence between producer and consumer, because the mutual confidence is a major thing to sustain the organic farming. In products marketing, it is more need for the information of organic farming products. Nowaday most products are marketed in Seoul region, but it is not a good method. To search the consumer in near region is a good method for decrease the cost of conveyance and maintain the quality of products. The varieties of distribution structure of organic farming products are required. The products are sold to department store in large city and the prices are about 30% higher than normal products. But when those are supplied in near region, the prices of those are similar to normal products. Direct transactions in near regions is a good method for decrease the cost of conveyance and strength the mutual confidence between producer and consumer.

두 마리의 고양이에서 발생한 복막 심낭막 횡격막 허니아의 수술적 치료

윤영민,정성목 忠南大學校 動物醫科學硏究所 2011 動物醫科學硏究誌 Vol.18 No.1

Two cats (5 years, intact female, Turkish Angora (case1) & 1 year, intact female, Persian Chinchinra (case2) admitted with the hisoty of cardiac abnormality. Two cats had the history of panting and increased respiratory rate. On physical examination, they had panting and right muffled heart sound. Thoracic radiography revealed increased cardiac silhouette, dorsal deviation of trachea, cylindric gas superimposition in cardiac silhouette, convex projection of the caudal cardiac silhoutte, and confluent silhoutte between heart and diaphragm. In ultrasonography, abdorminal viscera in pericardial sac were shown in two cats. They were diagnosed as peritoneopericardial diaphragmatic hermia(PPDH) based on diagnostic imaging examination. Herniorrhaphies and close perioperative care were performed for prevention of postoperative complication in two cats. PPDH in two cats were succesfully treated without postoperative complication.

부산광역권의 장기예측과 경제분석을 위한 다지역계량경제모형의 개발

윤성민,정병우,조광조,이영준 한국경상학회, 한국국민경제학회 2006 경제연구 Vol.24 No.1

가구주 연령별 최저생계비의 계측과 분석

윤성민 釜慶大學校 1997 釜慶大學校 論文集 Vol.2 No.1

This study is to develop the measurement model of minimunm cost-of-living and to measure and analyze the minimum cost-of-living by householder's ages in Korea. The previously used models have the advantage of easy understanding, but also have the serious weakness as follows :(a) The previous models can not produce the reference data for living wages in the social wage system, because those are to measure minimum cost-of-living by family size(not by householder's ages). (b)In the previous models the definition of the minimum cost-of-living is not clear,so the minimum cost-of-living is measured arbitrarily. (c) The previous models use the Rowntree method. But this method is not objective, so the measurement results using that method can not be agreed by the wage negotiating bodies. A new model suggested in this study uses a duality relation of consumer's preference. And it modifies a theoetical model that is developed by KaKwani(1977) and van der Gagg and Smolensky(1982). We measured the minimum cost-of-living by householder's ages in Korea with a raw data of「Annual Report on the Family Income and Expenditure Survey」by using that model and analyzed the results. The new method presented in this paper is not arbitrary and can be agreed by the wage negotiating bodies.