複合 韓藥劑 淸籬滋坎湯의 抗癌效果 및 作用機轉 糾明

Cho, Kyung-Sam,Kim, Si-Young,Park, Jai-Kyung,Choi, Seung-Hoon,Chung, Se-Young,Yoon, Hwi-Joong 경희대학교 동서의학연구소 1999 INTERNATIONAL SYMPOSIUM ON EAST-WEST MEDICINE Vol.1999 No.1

Kyung Sam Cho¹Si Young Kim¹, Jai Kyung Park²,Seung Hoon Choi³,Se Young Chung⁴, Hwi Joong Yoon¹¹College of Medicine, ²East-West Medical Research Institute, ³College of Oriental Medicine, ⁴College of Pharmacology, Kyung Hee University,Seoul, Korea. The Anti-cancer Effect of Oriental Medicine Chung-Ri-Ja-Gam-Tang in the Mouse with Metastatic Lung Cancer. Proceedings of International Symposium on East-West Medicine, Seoul. 231-243, 1999.-The oriental medicine Chung-Ri-Ja-Gam-Tang is an herbal medicine which has been used for pulmonary diseases and known as having immune stimulatory effects. It has been known effective in lung cancer. So we studied the effects and the mechanisms of this herbal medicine it the mouse with metastatic lung cancer. The metastatic lung cancer of the mouse was produced with melnoma cell line(B16BL/6).5×10□/mouse tumor cells were injected intravenously to the CDF1 mouse via tail vein. The mice were divided 4 groups. The first group was treated with 50mg/kg extract of Chung-Ri-Ja-Gam-Tang per oral for 10 days after cancer cell injection, second group treated with saline after cancer cell injection, the third group with medicine without cancer cell, and the last group with saline only. After 10 days treatments some of the mice were scarificed and the lung and spleen was removed. The survival duration, weight change, the number of metastatic cancer nodule of the lung, the NK cell activity, the capacity of cytokines(INF-γ,INF-α,IL-2) production and the proliferation activities of mouse lymphocytes were measured. The surival times of the group 1 mice were longer and the weight loss was less than the group 2 significantly. The number of the metastatic nodule of the lung were decresed in the group 1 than the group 2. The production of INF-γ,was increase in group 2 than group 3 and 4, IL-2 production was increased in group 1 than group 2,3,4 significantly. There was no difference in TNF- α production and proliferation activity of lymphocyte in each group. The NK cell activity was significantly increased in group 1 than group 2,3,4. We conclude that the Chung-Ri-Ja-Gam-Tang has the effect of increasing the NK cell activity of the CDF1 mouse with metastatic lung cancer(B16BL/6). And it is suggested that the increased production of the IL-2 is the mechanisms of enhanced NK cell activity.

임신 중 체중증가 정도에 따른 체성분 비교연구

조정훈 ( Jung-hoon Cho ),장준복 ( Jun Bock Jang ),김승보 ( Seung Bo Kim ),이경섭 ( Kyung Sub Lee ) 한방비만학회 2002 한방비만학회지 Vol.2 No.1

Objectives: To compare the body composition of the postpartal female weight-gained over the recommended during pregnancy with that of normal female with same age, female diagnosed as obesity and postpartal female weight-gained within the recommended during pregnancy. Methods: From Apr. 1. 2001 to Feb. 28. 2002, there were 745 delivery in Dept. of Obstetrics & Gynecology, Kyung-Hee Medical Center. 32 mothers of them wanted to be investigated about the weight gain during their pregnancy. We excluded 12 persons who had gained within the recommended weight(15㎏) and had diagnosed as the diseases inducing pathologic edema, preeclamsia, gestational heart or renal diseases, for example. In 2 days after delivery(nomal) or 5 days after(Caesarean section) we analysed the body composition of 20 persons. Results: 1. Compared with normal female, body weight, body mass index and body fat of the women gained over the recommended were increased and there made alternation to the increase of the percentage of body fat or the decrease of the percentage of lean body mass. 2. Compared with obese women who had same body mass index, the women gained over the recommended showed a little increase of body fluid, protein and mineral, but had a little decrease of the percentage of body fat. 3. The percentages of body fat increased with the women gained over the recommended, the women gained within the recommended and the obese women in order. This could imply the relation between the pregnancy, weight gain during pregnancy and the obesity. 4. The weights before pregnancy were same in the women gained over the recommended and the women gained within the recommended. But the weights just after delivery kept the gap happened during pregnancy between them and its main cause was the increase of body fat.

강남경희한방병원에 내원한 냉증을 호소하는 여성의 Heart Rate Variability 특성 연구

이미주 ( Mi Joo Lee ),김은경 ( Eun Kyung Kim ),이진무 ( Jin Moo Lee ),조정훈 ( Jung Hoon Cho ),장준복 ( Jun Bock Jang ),이경섭 ( Kyung Sub Lee ),이창훈 ( Chang Hoon Lee ) 대한한방부인과학회 2011 大韓韓方婦人科學會誌 Vol.24 No.3

구내용 분광광도계를 이용한 자연치의 색상분석

조경모,신동훈 大韓齒科保存學會 1998 Restorative Dentistry & Endodontics Vol.23 No.1

The recognition of the esthetics as well as their function of the teeth in the dental field is growing. Evaluating the esthetics of a tooth concerns shape, texture, and color. And among these, color has been the most difficult element to make natural expression. Color is a very subjective element affected by many different factor like light source, object, observer. Numerous efforts to quantifying color has been made. Currently the use of colorimeter and spectrophotometercolor that the color measuring device has been suggested. In this study, the normal range of the tooth color in canines, lateral incisors and central incisors for Koreans was evaluated with a modified spectrophotometer for intraoral use. The color differences in terms of teeth positions, age and area within the tooth was evaluated and comparatively analyzed. Results were as follow ; 1. In all age groups, the central incisors are most bright and least yellowish opposit to the canines are most dark and yellowish. The central incisors and lateral incisors showed the similar color patterns. 2. In the comparison between gender, it showed that in the 10's group male have more yellowish, the 20's female have lighter and more redness, the 30's indicated more redness in female and in the 40's the lightness is more in female. 3. With aging, there was no significant change in L^* and a^* coordinates but a shift was in b^* to yellowish in the 30's and 40's in central incisors. Lateral incisors and canines showed a lighter and more yellowish tooth color with an irregular change of color coordinate in a^*. 4. In all teeth the middle and cervical portion showed close correlation with over 0.7(0.05>p) but color (a^*, b^*) showed higher correlation than lightness(L^*). 5. It has been shown that through the regression test the cervical color could be calculated by the middle color value.

레니텍^(�) 정(말레인산 에날라프릴, 10mg)에 대한 에나레이스 정의 생물학적 동등성

조성희,하용화,홍성제,서성훈,류재환,김동현,이경태 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.3

The purpose of present study was to evaluate the bioequivalence of two enalapril maleate tablets, Renitec^(™) (MSD Korea Ltd.) and Enalace ^(™) (Welfide Korea Ltd.), according to the guidelines of Korea Food Drug Administration (KFDA). Twenty-four normal male volunteers, 22.33±2.55 year in age and 66.54±8.30 ㎏ in body weight, were divided into two groups and a randomized 2×2 cross-over study was employed. After two tablets containint 10 ㎎ of enal-april maleate per tablet wre orally administered, blood was taken at predetermined time intervals and concentrations of enalapril in plasma were determined using LC-MS-MS. Pharmacokinetic parameters such as AUC_(t), C_(max) and T_(max) were calculated and ANOVA test was utilized for the statistical analysis of the parameters using logarithmically transformed AUC_(t) and C_(max) untransformed T_(max). There were no sequence effects between two formulations in these parameters. The 90% confidence intervals for the log transfored data were acceptance range of log0.8 to log1.25(e.g., log1.02∼log1.14 and log1.03∼log1.19 for AUC_(t) and C_(max), respectively). The major parameters, AUC_(t) and C_(max), met the criteria of KDFA for bioequivalence indicating that Enalace^(™) tablet is bioequivalent to Renitec^(™) tablet.

다중 제어루프에 의한 자기부상용 DC-DC 전원장치에 관한 연구

조주현,조정민,전기영,이상집,이승환,오봉환,이훈구,한경희 明知大學校 産業技術硏究所 2004 産業技術硏究所論文集 Vol.23 No.-

The author present a modified multi-loop algorithm including feedforward for controlling a 55kW step down chopper in the power supply of Maglev. The control law for the duty cycle consists of three terms. The first is the feedforward term which compensates for variations in the input voltage. The second term consists of the difference between the slowly moving inductor current and output current. The third term consists of proportional and integral terms involving the perturbation in the output voltage. This perturvation is derived by subtracting the desired output voltage from the actual output voltage. The proportional and integral action stabilizes the system and minimizes output voltage error. In order to verify the validity of the proposed multi-loop controller, simulation study was tried using Matlab simulink.

역전사 중합효소 연쇄반응을 이용한 사람의 활성 T세포 항원 CTLA-4 유전자 클로닝 및 마우스 L세포에서의 발현

조양자,장경덕,정용훈,박장환,김경희 한양대학교 의과대학 1993 한양의대 학술지 Vol.13 No.2

Occupancy of the T-cell receptor (TCR) by antigen in association with class Ⅱ MHC molecule is required for the initiation of T cell activation. However TCR stimulation is not a sufficient signal to account for all the observed events that occur during T cell activation. And in the absence of a second costimulatory signal provided by abtigen presenting cell antigen specific signal may lead to clonal inactivation or energy. CD28, a T cell surface molecule first defined by monoclonal antibody 9.3 on human cells, appears to function as an alternative, TCR/CD3-independent, activation pathway for T cell. This molecule provides costimulatory signal for T cell proliferation by increasing a number of lymphokines and cytokines production. A T cell activation antigen CTLA-4 is homologous to CD28 with 28% amino acid and 67% of nucleic acid homology in the protein coding region. CTLA-4 was ofiginally identified by the differential screening follosing subtractive hybridization of murine cytotoxic T cell cDNA library and mapped to the same lacation on chromosome 2 in human and chromosome 1 in mouse as CD28. These findings and activation associated expression pattern of CTLA-4 raise question about functional role of this molecule in T cell activation. In this study human CTLA-4 cDNA was amplified from phytohemagglutinin (PHA)-stimulated human peropheral blood lymphocytes mRNA by using reverse transcription-ploymerase chain reaction (RT-PCR) method. And amplified human CTLA-4 cDNA was subsequently cloned into pRC/CMV vector. In double stranded DNA sequencing the 331st base of protein coding region was changed from guanylic acid of human CTLA-4 from alanine to threonine. Murind L cell transfected with this clone expressed 36kD protein. It appears that this dirrerence of molecular weight between native (34kD) and recombinant (36kD) human CTLA-4 molecule may due to glycosylation differences. The cDNA and murine CTLA-4 molecule may due to glycosylation differences. The cDNA and murine L cell lines expressing human CTLA-4 developed by this study would contribute in future work unveiling the biological rile of CTLA-4 in T cell activation.

peat moss를 이용한 폐수중의 중금속 제거

이경화,김정섭,유현철,최성우,윤철종,우성훈,박승조 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

Adsorption equilibrium of heavy metals ion on the peat moss was confirmed Frundlich isotherm Removal efficiency of heavy metals ion was increased from pH 2 to 10 and heavy metal removal efficiency was increased in the sequence of Cu(Ⅱ) > Pb(Ⅱ) > Cd(Ⅱ) > Mn(Ⅱ) on the PM(Peat Moss). The PM is likely to be more effective adsorption than the PAC to removal of metal ions in the solution, since PM's weakly acidic functional groups which is not founded on the PAC, in addition to its high surface area and porosity. But, the PM is not separated from the solution water by sedimentation process. So, a liquid and solid seperation between the PM and the solution water could be considered for the PM's field application. But peat moss was low cost adsorbent. Thus author propose to adopt PM as a adsorbent for heavy metal removal.

페탄광 주변 하천의 수질조사

정경훈,최형일,정오진,정재경,강성환,조영찬 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

The Water quality for river surrounding closed coal mine in Hwasun area Chunnam was investigated. The investigated factors were pH, turbidity, conductivity, Fe, SO_(4)^(-2), Ca, and Mg concentration, and the crystallization form and components of suspended solids were also investigated by scanning electron microscope and energy dispersion system Extremely high value of turbidity and conductivity were represented in Honam Kwangup (N-7) and Honam Tanza (N-3)area, respectively. Fe concentration was lower in all sampling sites, and the concentration of SO_(4)^(-2) ion was higher than 200mg/L in all sampling sites excepting Dongyang Tanggwang area (N-2) and Hwail wetland (N-11). The SEM micrograph and EDS analysis showed that the crystallization form of the suspended solids was formless, These particles have size of less than 10??and the main components was O, Al, Fe and S. The corelation values between conductivity and Mg, SO_(4)^(-2), and Ca were 0.967, 0.959, 0.931, respectively, and the results indicate good corelation.

접착제와 와동형성의 차이에 따른 5급 복합레진 수복의 전향적 임상연구

이경욱,정세준,한영철,손호현,엄정문,오명환,조병훈 大韓齒科保存學會 2006 Restorative Dentistry & Endodontics Vol.31 No.4

본 연구의 목적은 복합레진을 이용한 비우식성 치경부 5급 병소 수복에 있어서, 3가지 다른 접착제를, 유지구를 부여한 치아와 부여하지 않은 치아에 적용하였을 때의 임상적인 효능의 차이를 비교하기 위함이다. 총 150개의 치아를 각각 25개씩 6개의 군으로 나누었다. A군 Scotchbond Multi-Purpose (SBMP, 3M ESPE, St, Paul, MN, USA, 4세대 접착제)를 사용하고 유지구를 부여하지 않음. B군: SBMP를 사용하고 유지구를 부여. C군: BC Plus (Vericom Co., Anyang, Gyeonggido, Korea, 5세대 접착제)를 사용하고 유지구를 부여하지 않음. D군: BC Plus를 사용하고 유지구를 부여. E군: Adper Prompt (3M ESPE, Seefeld, Germany, 6세대 접착제)를 사용하고 유지구를 부여하지 않음. F군: Adper Prompt를 사용하고 유지구를 부여. 모든 치아는 복합레진인 Denfil(Vericom Co., Anyang. Gyonggido. Korea)을 이용하여 충전하였다. 수복 직후와, 수복 6개월 후에 modified USPHS (United States Public Health Service) criteria에 따라 수복물을 임상적으로 평가하였다. 추가적으로 임상사진을 촬영하였으며, 에폭시 레진을 이용하여 복제한 후 주사전자현미경을 이용하여 이를 관찰하였다. 6개월 후 관찰 결과. alpha rating의 수가 각 실험군마다 동일하지는 않았지만, 통계적으로는 3가지의 접착제간에 유의할만한 차이는 없었고, 기계적인 유지구를 부여한 군과 부여하지 않은 군 간에도 유의할만한 차이는 없었다 (p > 0.05). 그러나 6개월의 관찰 기간은 일반적으로 기대되는 레진의 수명보다 짧기 때문에, 각 실험군 간의 차이를 알아보기 위해서는 추후 더 오랜 기간의 관찰이 요구된다. The purpose of this study is to evaluate prospectively the effect of different bonding systems and retention grooves on the clinical performance of resin restorations in non-carious cervical lesions (NCCLs). Thirty nine healthy adults who had at least 2 NCCLs in their promolar areas were included in this study. One hundred and fifty teeth were equally assigned to six groups; (A) Scotchbond Multi-Purpose (SBMP, 3M ESPE, St, Paul, MN, USA, 4th generation bonding system) without retention grooves; (B) SBMP with retention grooves: (C) BC Plus (Vericom Co., Anyang, Gyeonggido, Korea, 5th generation bonding system) without retention grooves; (D) BC Plus with retention grooves; (E) Adper Prompt (3M ESPE, Seefeld, Germany, 6th generation bonding system) without retention grooves: (F) Adper Prompt with retention grooves. All cavities were filled with a hybrid composite resin, Denfil (Vericom Co., Anyang, Gyeonggido, korea) by one operator. Restorations were evaluated at baseline and at 6-month recall, according to the modified USPHS (United states Public Health Service) criteria, Additionally, clinical photographs were taken and epoxy resin replicas were made for SEM evaluation. At 6-month recall, there were some differences in the number of alpha ratings among the experimental groups. But, despite the differences in the number of alpah ratings, there was no significant difference among the 3 adhesive systems (p > 0.05). There was also no significant difference between the groups with or without mechanical retention (p > 0.05). Follow-ups for longer periods than 6 months are needed to verify the clinical performance of different bonding systems and retention grooves. 〔J Kor Acad Cons Dent 31(4):300-311, 2006〕