흰쥐의 방사선 피폭후 생물학적 지표로서의 혈액효소

김진규,김상복,김국찬,천기정,김인규,박효국,이강석 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

생물학적 선량평가를 위한 생화학적 지표 연구로서 흰쥐 혈액내 효소활성도의 변화를 조사한 결과는 다음과 같다. 1) Alkaline phosphatase 활성도는 0.1, 0.25, 0.5, 2, 4 Gy의 방사선 조사후 24시간까지 혈액내 활성도가 증가하였고 72시간 경과시에는 대조군과 비슷한 활성도를 보였다. Creatine kinase는 2, 4 Gy 방사선 조사후 혈액내에서 활성도에서 활성도가 72시간까지 증가하였으나 0.1, 0.25Gy, 0.5 Gy 방사선 조사시에는 커다란 변화를 보이지 않았다. 2) Malate dehydrogenase 활성도는 0.1, 0.25, 0.5 Gy 방사선 조사시에는 커다란 변화가 없었으며 lactate dehydrogenase는 방사선 조사후 활성도가 감소하였다. 3) GOT의 활성도는 선량률 0.1Gy/min.로 0.1, 0.25, 0.5, 2, 4 Gy 조사후에는 어떠한 변화도 없었으며 선량률 0.5 Gy/sec.로 0.5, 1, 1.5, 2, 3, 5, 7 Gy로 조사후에는 증가현상을 보이고 있다. Acid phosphatase 활성도는 상기의 어떠한 선량에서도 나타나지 않고 있다. 잠재적으로 이러한 효소들은 방사선 피폭의 지표물질로 사용될 수 있으며 생화학적 지식과 기술을 이용한 좀더 나은 지표물질을 찾기 위하여 계속적인 조사가 필요하다. Enzyme activity changes in rat blood as biochemical indicator useful for evaluating exposure dose were experimentally studied. The experimental results obtained are as follows: 1) Alkaline phosphatase activities increased in the blood serum until 24 hours after 0.1, 0.25, 0.5, 2 and 4 Gy irradiation and its activities returned mormal condition after 72 hours of post-irradiation. Creatine kinase activities increased in the blood serum until 72 hours after 2 and 4 Gy irradiation but any significant activity changes were not detected after 0.1, 0.25 Gy irradiation. 2) Malate dehydrogenase activities did not reveal available changes changes after 0.1, 0.25, 0.5 Gy irradiation and lactate dehydrogenase activities decreased in the blood serum after 0.1, 0.25, 0.5 Gy irradiation. 3) Glutamate oxaloacetate transaminase activity changes were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and GOT activities increased after 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.). Any acid phosphatase activities were detected in the blood serum after 0.1, 0.25, 0.5, 2, 4 Gy(0.1 Gy/min.) and 0.5, 1, 1.5, 2, 3, 5, 7 Gy(0.5 Gy/sec.) irradiation. Potentially some of these enzymes can be used as indicator protein for radiation injury. Futher investigation is needed to find better biochemical indicatiors utilizing recent knowledge and techniques of biochemistry.

우라늄 투여후 간조직에서의 효소활성도의 변화

김국찬,이강석,김진규,김인규,박효국,천기정,김상복 대한방사선 방어학회 1993 방사선방어학회지 Vol.18 No.2

우라늄 피폭후 효소활성도가 내부피폭의 biomarker로서의 가능성을 연구하기 위하여 수서생물인 잉어9Caprinus carpio)의 복강내 우라늄 투여시 간조직에서 일어나는 여러가지 효소계의 변화를 조사하였다. 이러한 효소활성도의 변화는 우라늄 내부피폭의 biomarker로서 가능성을 가지고 있으며 연구결과는 다음과 같다. 1) 6일째까지 우라늄을 주사하여 적출한 간에서의 전체단백질량은 계속 감소하고 있다. 2) 세포내의 lysosome내에 함유하고 있는 acid protease와 β-glucuronidase의 활성도는 6일째 투여후까지 활성도가 감소하고 있다. 3) Alkaline phosphatase의 경우 6일째 우라늄 투여후까지 증가하고 있으며 반대로 acid phosphatase의 경우 6일째 우라늄 투여후까지 활성도가 급격히 증가하였고 glutamate oxaloacetate transaminase의 활성도는 완만하게 증가하고 있다. 4) Creatine kinase의 활성도는 완만한 감소를 보이고 있으며 malate dehydrogenase는 첫번 우라늄 투여후에 활성도가 급격히 감소하였고 3일째 우라늄 투여후에는 활성도가 거의 나타나지 않았다. We examined various enzyme activity changes by intraperitioneal injection uranium in the carp liver. These enzyme activity changes can be used as biochemical indicators of internal exposure to uranium. The results were followings ; 1) Total protein concentration decreased by intraperitoneal injection in the carp liver. 2) Lysosomal acid protease and β-glucronidase activities increased in the liver until sixth intraperitoneal injection of uranium, but lysosomal acid phosphatase activities decreased in the liver until the sixth injection of uranium. 3) Alkaline phosphatase activities sharply increased and Glutamate oxaloacetate Transaminase activities steadily decreased in the liver until the sixth injection of uranium. 4) Creatine %kinase activities steadily decreased and malate dehydrogenase activities sharply decreased in the liver after the primary injection of uranium. Any malate dehydrogenase activities was not detected after sixth injection of uranium.

Ki-1 임파종 1 예

김용진,김경재,박재복,이지현,안기성,강민모,최석문,황기석 대한내과학회 1993 대한내과학회지 Vol.45 No.4

A 60-year-old man was admitted to our hospital complaining of fever with chillness and anarexia for 12 weeks. Physical examination revealed supraclavicular lymphadenopathy and left inguinal lymphadenopathy. Abdominal CT scan showed the evidence of maked lymphadenopathy on retraperitoneal, external iliac, obturator, left inguinal, and celiac lymph nodes. A pathologic diagnosis of Ki-1(+) lymphoma was made by the biopsed supraclavicular lymph node because the node consisted of large cells with pleomorphic nuclei, scanty cytoplasm, large basophilic nucleoli, atypical vacuoli and large cells were positive for leukocyte common antigen, Ber-H2, marker, and B-cell marker.

초음파를 이용한 바이오시스템 계측

김기복,김만수 한국산업식품공학회 2004 산업 식품공학 Vol.8 No.1

Ultrasonic nondestructive testing is a versatile an successful technique that can be applied to a wide variety of material analysis applications. The state-of art techology of sensors, microprocessors, and methods for signal analysis hs been promising new possiblilities for the application of ultrasonic techniques to non-destructive and non-hazardous tstings. Ultrasonic material analysis is based on a simple principle of physics. That is, the motion of a wave is affected by the medium through which it travels. Thus, changes in one or more of four measureable parameters associated with the passage of a elastic wave through a material - time of flight, attenuation, scattering, and frequency content - would be correlated with changes in physical properties such as hardness, elastic modulus, density, homogeneity or particle size and structure. Ultrasonic techniques can be applied to various biosystems such as food, agricultural products and biological materials, owing to their nondestructive nature. in this paper, the principle of ultrasonic technique was briefly described and its applications to biosystems were reviewed.

?? 착물의 구조적 특성

김복조,윤두천,박기훈,주언정,오창언 嶺南大學校 基礎科學 硏究所 1998 基礎科學硏究 Vol.18 No.-

Five-coordinated complex of Cu(Ⅱ) complex, [CuCl(1,10-phenanthroline)₂]·Cl·6H₂O·CH₃OH, was synthesized by adding an aqueous solution of Cu(Ⅱ) to a methanol solution of 1,10-phenanthroline. Its crystal structure was detemined by single crystal X-ray crystallography. Crystal data are are as fllow: [CuCl(phen)₂]·Cl·6H₂O·CH₃OH, monoclinic, space group C2/c (#15), a = 23.309(5), b = 30.270(6), c = 7.491(2) A, B = 97.83(2)˚, V = 5236 A³, Z = 8,2745 data with I >3σ(I) (R = 00.64, Rw = 0.087). In the crystal structure of ??, the Cu(Ⅱ)is five-coordianted with four nitrogen and one chlorine atom. The geometry around Cu(Ⅱ) is somewhat distorted trigonal bipyramid and Cu(Ⅱ) is locted nearly (0.0280 A˚) on the least-squares plane of the two nitrogen atoms and one chlorine atom.

미세가공 정전용량형 초음파 탐촉자 개발 (Ⅰ) : 진동 막 거동 분석 Analysis of the Membrane Behavior

김기복,안봉영,박해원,김영주,이승석 한국비파괴검사학회 2004 한국비파괴검사학회지 Vol.24 No.5

본 연구는 고효율 비접촉 초음파 송수신이 가능한 미세가공 정전용량형 츠음파 탐촉자(cMUT)를 개발하기 위한 기초연구로 수행되었다. cMUT의 주요 구성요소인 미세 진동 막의 거동-공진 주파수, 막 번위, 막 붕괴 변위, 막 붕괴전압을 이론적인 해석과 유한요소해석을 이용하여 분석하였다. 진동 막의 두께와 크기, 회생층 두께, 전극의 두께와 크기 등과 같은 cMUT의 설계인자를 검토하였다. 분석결과 진동 막의 공진주파수는 진동 막의 두께가 증가함에 따라 증가하였으며 직경에 따라서는 감소하였다. 직류 마이어스 전압이 증가함에 따라 진동 막의 변위는 증가하였으며 진동 막의 붕괴전압을 분석하였다. This study was conducted to develope a capacitive micromachined ultrasonic transducer (cMUT) which enable to high efficient non-contact transmit and receive the ultrasonic wave in air. Theoretical analysis and finite element analysis of the behavior of membrane (such as resonance frequency, membrane deflection, collapse deflection and collapse voltage) of the cMUT were performed. The design parameters of the cMUT such as the dimension and thickness of membrane, thickness of sacrificial layer, thickness and size of electrode were estimated. The resonance frequency of the membrane increased as the thickness of the membrane increased but decreased as the diameter of the membrane increased. The deflection of the membrane increased as d-c bias voltage increased. The collapse voltage of the membrane was analyzed.

고 조리서에 수록된 만두의 종류와 조리법에 관한 고찰 : 1600년대부터 1950년대까지 발간된 고 조리서를 중심으로 Focused on the Old Cooking Books issued in 1600 to 1950

김기숙,이미정,한복진 동아시아식생활학회 1999 동아시아식생활학회지 Vol.9 No.1

Motivated by a need to provide the basic data of standard recipes for Korean traditional food, Mandoo, this paper explores a historic research about the development of Mandoo classified by Mandoo-crust, Mandoo-filling, spice & condiment, garnish, size & shape and unit for measuring ingredients. The data for this study are obtained from the major old cooking books published in the period 1670-1957, such as 「Eumsikdimibang」, 「Leejogoongjoungyoritonggo」, and so on. The results of this study show that (1) starch, buckwheat flour and flour were used as Mandoo-crust and egg was added in order to improve cohesion, (2) cooked pheasant and beef were used as Mandoo-filling, (3) ginger had been used more generally than garlic as spice & condiment and later red pepper powder was added and pine nut was also widly used, (4) pan-fried meat, egg, and mushroom were used as garnish, (5) Mandoo of different kinds existed and had various sizes and shapes, (6) unit for measuring ingredients began to appear on printing in 1939. These findings provide us with opportunity, which leads to making the standard recipes for Mandoo so that anyone can easily have resources for cooking the traditional food, Mandoo.

마하피닝 처리에 의한 기계구조용 합금강의 피로균열전파 지연효과

김민건,임복규,박홍기,황중각 강원대학교 산업기술연구소 2006 産業技術硏究 Vol.26 No.B

Mach peening treatment is one of the various kinds of techniques to improve the fatigue properties. The mach peening process gives high-level work hardening and compressive residual stress near the surface layer, improving the fatigue strength. In addition, this treatment reduces slip bands that initiate the fatigue cracks near the surface. During impingement, a plastic indentation surrounded by a plastic zone is formed. Mach peening treatment characteristic is less energy consumption and is an environmental friendly processing methods that is not accompanied by pollution. It is machining process that can prevent fatigue fracture beforehand in structure using already as well as process of production. The test results showed that fatigue crack propagation delay appeared by drilling type 43%, mach peening type 110%.

청주 및 충북지역소재 무역상사의 최신개정 무역거래조건 및 신용장 통일규칙의 보급이용도측정과 그들 상사사용무역영어적절성에 관한 연구

김복문,고석하,백대기,김주엽 충북대학교 산업경영연구소 1991 産業과 經營 Vol.4 No.2

대도시 청소년 체격의 발육발달과 표준체중에 관한 연구

김성만,손흥기,김복현 경희대학교 체육과학연구소 1995 體育學論文集 Vol.23 No.-

The Purpose of this study is to analyzing the developing process of physique during the 12 years regression equation standard body weight according to the age and body height and also we analyzed the distribution status of normal body weight and obesity weight in 17-year old high school students. A total of 1,423 samples(male:723, female: 701, born between March 1, 1977∼February 28, 1978) and the students who were randomly selected in big city of north of Jeola province, The result of our analysis are as follows: 1.Measurement of Physique ①The steady growth showen 14 year of male and 12-year of female in body height, 14-year of male and 13-year of female In body weight, 15-year of male and 13-year of female In chest-girth, since then the growth and development linear curve is more showness. ②During the 12year of school age, the rate of growth were 55.12cm(47.4%) of male 44.80cm(38.9%) of female in body height 43.59(214.5%) of male, 35.20kg(180.8%) female in body weight and 3.83cm (55.48%) of male, 28.11cm(50.70%) of female in chest girth. ③The age of maximum rapids growth were 12∼13 years old(7.36 ± 2.52cm) of male 10∼ 11years old (6.81± 1.88cm) of female In body height, 12∼13years old(6.14±3.37kg) of male, 11∼12 years old(5.49±2.67kg) in body weight and the chest-girth were 13∼14years old(4.34± 3.20cm) in male, 11∼12 years old(5.01 ± 3.80cm) in female. ④The age of cross over between male and female were 9.7∼ 11.7 years old in body height, 11.5∼ 13.5 years old in chest-girth and there is on cross over in body weight. ⑤The body height of 17-year old was 171.68∼5.56cm in mate and 159.92±4.85cm in female, body weight was 63.91±8.89kg in male and 54.67±6.74kg in female and the chest-girth was 89.20±5.94cm in male 83.55 ± 5.27cm in female. ⑥Physical growth and development was more remarkable improvement than 1980' Physique especially the age of adolescence. 2.Standard body weight by regression equation ①Correlation coefficient of r=0.469-0.760 between body height and body weight in male and r=0.347-0.793 in female. ②Regression equation of standard body weight were found in 17-year old and the equation were y =0.749X+64.791 in male, y=0.484X+22.800 in female. ③The distribution of normal body weight were 63.1% in male and 76.2% in female, over weight were 10.4% in male and 9.3% in female and the obesity weight were 6.9% in male and 3.4% in female.