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한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률
조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4
Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.
Kang, Eun-Ju,Byun, June-Ho,Choi, Young-Jin,Maeng, Geun-Ho,Lee, Sung-Lim,Kang, Dong-Ho,Lee, Jong-Sil,Rho, Gyu-Jin,Park, Bong-Wook Mary Ann Liebert, Inc 2010 Tissue engineering. Part A Vol.16 No.3
<P>In vitro and in vivo osteogenesis of skin-derived mesenchymal stem cell-like cells (SDMSCs) with a demineralized bone (DMB) and fibrin glue scaffold were compared. SDMSCs isolated from the ears of adult miniature pigs were evaluated for the expression of transcriptional factors (Oct-4, Sox-2, and Nanog) and MSC marker proteins (CD29, CD44, CD90, and vimentin). The isolated SDMSCs were cocultured in vitro with a mixed DMB and fibrin glue scaffold in a nonosteogenic medium for 1, 2, and 4 weeks. Osteonectin, osteocalcin, and Runx2 were expressed during the culture period and reached maximum at 2 weeks after in vitro coculture. von Kossa-positive bone minerals were also noted in the cocultured medium at 4 weeks. Autogenous porcine SDMSCs (1 x 10(7)) labeled with a tracking dye, PKH26, were grafted into the maxillary sinus with a DMB and fibrin glue scaffold. In the contralateral side, only a scaffold was grafted without SDMSCs (control). In vivo osteogenesis was evaluated from two animals euthanized at 2 and 4 weeks after grafting. In vivo PKH26 staining was detected in all the specimens at both time points. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the SDMSC-grafted group compared with the control group. New bone generation was initiated from the periphery to the center of the grafted material. The number of proliferating cells increased over time and reached a peak at 4 weeks in both in vivo and in vitro specimens. These findings suggest that autogenous SDMSC grafting with a DMB and fibrin glue scaffold can serve as a predictable alternative to bone grafting in the maxillary sinus floor.</P>
04 포스터 발표 : 토양 환경 분야(PS) ; PS-12 : 서로 다른 토양에서 Imidacloprid의 흡착 및 탈착 특성
이창호 ( Chang Ho Lee ),남지윤 ( Ji Youn Nam ),권회군 ( Hoe Gun Kwon ),이제형 ( Je Hyeong Lee ),송은희 ( Eun Hee Song ),추환용 ( Hwan Yong Choo ),맹은호 ( Eun Ho Maeng ) 한국환경농학회 2015 한국환경농학회 학술대회집 Vol.2015 No.-
Imidacloprid는 chloronicotinyl계 침투성 살충제로서 흡입해충(sucking insects), 토양해충(soil insects),흰개미(termites), 저작해충(chewing insects) 등을 방제할 목적으로 사용되고 있다. 그러나, 꿀벌(Apismellifera)에 대하여 독성이 매우 높고, LD50이 8 ng/bee로 보고 되어 있다. 그리고, 토양내에서 imidacloprid의 수착(sorption) 정도는 유기탄소 및 무기물질 등의 영향을 많이 받는 것으로 알려져 있다. 따라서, 본 연구에서는 서로 다른 토양에서 imdacloprid의 흡/탈착특성을 알아보고, 토양내 거동형태를 파악하고자 하였다. HCB-SL-PF (silty loam, pH 6.8, OC 33.0 g kg-1), Iowa Soil (silty loam, pH6.2, OC 8.5 g kg-1)의 두 종류의 토양을 선정하였으며, 흡/탈착실험을 위하여 토양과 용액비율을 1:3(w/v)으로 하여 Imidacloprid를 0, 5, 10, 50, 100, 500 mg kg-1 수준으로 처리하여 상온에서 20 rpm의 속도로 4시간 진탕하였다. 산출된 결과를Freundlich equation에 적용한 일차식으로 나타내었고, 흡착실험에서 HCB-SL-PF 토양은 기울기 0.807 (R2 0.980), KadsF 1.897, Iowa Soil 토양은 기울기 0.950 (R20.998), KadsF 1.359로 나타났다. 탈착실험에서는 HCB-SL-PF 토양은 기울기 0.582 (R2 0.924), KadsF3.461, Iowa Soil 토양은 기울기 0.977 (R2 0.917), KadsF 1.390로 나타났다. 상대적으로 유기탄소함량이 높은 HCB-SL-PF (OC 33.0 g kg-1) 토양이 Iowa Soil (8.5 g kg-1) 토양보다 흡착계수가 높게 나타났다.
미니돼지에서 자가 피부유래 간엽성 줄기세포를 이용한 상악동저 거상술
변준호(June-Ho Byun),강은주(Eun-Ju Kang),맹근호(Geun-Ho Maeng),노규진(Gyu-Jin Rho),강동호(Dong-Ho Kang),이종실(Jong-Sil Lee),박봉욱(Bong-Wook Park) 대한구강악안면외과학회 2010 대한구강악안면외과학회지 Vol.36 No.2
Introduction: In our previous studies, we isolated porcine skin-derived mesenchymal stem cells (pSDMSCs) from the ears of adult miniature pigs and evaluated the pluripotency of these pSDMSCs based on expressions of transcription factors, such as Oct-4, Sox-2, and Nanog. Moreover, the characteristic of mesenchymal stem cells was revealed by the expression of various mesenchymal stem cell markers, including CD29, CD44, CD90, and vimentin. The aim of this study was to evaluate in vivo osteogenesis after maxillary sinus lift procedures with autogenous pSDMSCs and scaffold. Materials and Methods: The autogenous pSDMSCs were isolated from the 4 miniature pigs, and cultured to 3rd passage with same methods of our previous studies. After cell membranes were labeled using a PKH26, 1×107 cells/100 μL of autogenous pSDMSCs were grafted into the maxillary sinus with a demineralized bone matrix (DBM) and fibrin glue scaffold. In the contralateral control side, only a scaffold was grafted, without SDMSCs. After two animals each were euthanized at 2 and 4 weeks after grafting, the in vivo osteogenesis was evaluated with histolomorphometric and osteocalcin immunohistochemical studies. Results: In vivo PKH26 expression was detected in all specimens at 2 and 4 weeks after grafting. Trabecular bone formation and osteocalcin expression were more pronounced around the grafted materials in the autogenous pSDMSCs-grafted group compared to the control group. Newly generated bone was observed growing from the periphery to the center of the grafted material. Conclusion: The results of the present study suggest that autogenous skin-derived mesenchymal stem cells grafting with a DBM and fibrin glue scaffold can be a predictable method in the maxillary sinus floor elevation technique for implant surgery.
Nam, Eun-Joo,Lee, Young-Jae,Oh, Young-Ah,Jung, Jin-Ah,Im, Hye-In,Koh, Seong-Eun,Maeng, Sung-Ho,Joo, Wan-Seok,Kim, Yong-Sik The Korean Society of Pharmacology 2003 The Korean Journal of Physiology & Pharmacology Vol.7 No.6
3-Nitropropionic acid (3-NP) inhibits electron transport in mitochondria, leading to a metabolic failure. In order to elucidate the mechanism underlying this toxicity, we examined a few biochemical changes possibly involved in the process, such as metabolic inhibition, generation of reactive oxygen species (ROS), DNA strand breakage, and activation of Poly(ADP-ribose) polymerase (PARP). Exposure of SK-N-BE(2)C neuroblastoma cells to 3-NP for 48 h caused actual cell death, while inhibition of mitochondrial function was readily observed when exposed for 24 h to low concentrations (0.2${\sim}$2 mM) of 3-NP. The earliest biochemical change detected with low concentration of 3-NP was an accumulation of ROS (4 h after 3-NP exposure) followed by degradation of DNA. PARP activation by damaged DNA was also detectable, but at a later time. The accumulation of ROS and DNA strand breakage were suppressed by the addition of glutathione or N-acetyl-L-cysteine (NAC), which also partially restored mitochondrial function and cell viability. In addition, inhibition of PARP also reduced the 3-NP-induced DNA strand breakage and cytotoxicity. These results suggest that oxidative stress and activation of PARP are the major factors in 3-NP-induced cytotoxicity, and that the inhibition of these factors may be useful in protecting neuroblastoma cells from 3-NP-induced toxicity.
Ceramide is Involved in $MPP^+-induced$ Cytotoxicity in Human Neuroblastoma Cells
Nam, Eun-Joo,Lee, Hye-Sook,Lee, Young-Jae,Joo, Wan-Seok,Maeng, Sung-Ho,Im, Hye-In,Park, Chan-Woong,Kim, Yong-Sik The Korean Society of Pharmacology 2002 The Korean Journal of Physiology & Pharmacology Vol.6 No.6
To understand the cytotoxic mechanism of $MPP^+,$ we examined the involvement of ceramide in $MPP^+-induced$ cytotoxicity to human neuroblastoma SH-SY5Y cells. When SH-SY5Y cells were exposed to $MPP^+,\;MPP^+$ induced dose-dependent cytotoxicity accompanied by 2-fold elevation of intracellular ceramide levels in SH-SY5Y cells. Three methods were used to test the hypothesis that the elevated intracellular ceramide is related to $MPP^+-induced$ cytotoxicity: $C_2-ceramide$ was directly applied to cells, sphingomyelinase (SMase) was exogenously added, and oleoylethanolamine (OE) was used to inhibit degradation of ceramide. Furthermore, inhibition of ceramide-activated protein phosphatase (CAPP), the effector of ceramide, using okadaic acid (OA) attenuated cell death but treatment of fumonisin $B_1,$ the ceramide synthase inhibitor, did not alter the cytotoxic effect of $MPP^+.$ Based on these, we suggest that the elevation of intracellular ceramide is one of the important mediators in $MPP^+-induced$ cell death.