Carbon tetrachloride를 투여한 rat의 hepatic lipid 축적에 미치는 vitamin E의 효과에 관한 연구

박은주,이경연,이미영,이외숙,장재정,정귀은,최진희 曉星女子大學校 藥學大學 學生會 1988 曉星藥誌 Vol.4 No.-

The present studies were undertaken to evaluate the effect of vitamin E, CCl_4 on the change of hepatic triglyceride, hepatic cholesterol, hepatic phospholipid in male rat. The result obtained from this study were summarised as follows: 1. Hepatic phospholipid of CCl_4 treated rat was increased in proportion to CCl_4 dosage but after concomitant injection(I.P) of vitamin E and CCl_4, hepatic phospholipid was significantly decreased in comparison to that of CCl_4 alone injection. 2. There was no effect on hepatic cholesterol concentration either CCl_4 alone injection(I.P) or concomitant injecton(I.P) of vitamin E and CCl_4. 3. Hepatic triglyceride of CCl_4-treated rat was significantly increased in comparison to that of normal rat but hepatic triglyceride of rat concomitant injection of vitamin E and CCl_4 was significantly decreased in comparison to that of CCl_4 alone injection.

뇌동맥류를 진단하는데 침습적 뇌혈관 조영술이 자기공명 혈관 조영술보다 언제나 더 유리한가?

사은희,김대성,정대수,박규현,배지호,김학진 대한신경과학회 1998 대한신경과학회지 Vol.16 No.5

알칼리하에서 UV 흡수극대 파장 이동을 이용한 Nitrofurazone 정량

박송희,김영아,김은정 曉星女子大學校 藥學大學 學生會 1990 曉星藥誌 Vol.5 No.-

Nitrofurazone in exsiting method is determined by measuring absorbance in 375nm. This method is anxious about inhibition of 5-NO_2-furaldehyde derivatives and inconvenience of procedure. So we studied new determination method with shift of U.V maximum absorbance wavelength in alkali. 1. The shift of absorbance to a longer wavelength was extreamly in DMF, ethanol. 2. DMF-NF solution appeared constant absorbance in KOH concentration between 2.8 and 14×10^-3mole/l. EtOH-NF solution appeared constant absorbance in KOH concentration between 2.5 and 5×10^-2mole/l So this KOH concentration can be used determination of Nitrofurazone.

HBsAg 양성혈청에서의 HBeAg 및 anti-HBe 양성율과 ALT와의 관계 고찰

문희주,윤기은,박정오,배형준,최범열 서울보건대학 1996 서울보건대학 부설 한국보건과학연구소 논문집 Vol.3 No.1

The authors investigated HBeAg and anti-HBe in 1,000 cases(male 941, female 59) of HBsAg positive among the 225,512 blood donors in Seoul Nambu Blood Center in 1994. HBeAg and anti-HBe was detected by the method Enzyme Immunoassy. The results obtained were as follows ; 1. HBeAg and anti-HBe positive rates were detected 498 cases(49.8%) and 445 cases(44.5%) respectively among 1,000 cases HBsAg positive blood donors. 2. In HBsAg positive carriers, HBeAg positive rates were not significantly different between anti-HBe positive rates, but in sex were significantly different between HBeAg positive rates and anti-HBe positive rates. 3. HBeAg positive 498 cases were analyzed with age and it was found percentages of positive rates were 63.6% in 1st decade, 53.7% in 2nd decade, 20.6% in 3rd decade, 19.6% in 4th decade and 8.3% in over 5th decade, but anti-HBe positive 447 cases were 32.4% in 1st decade, 40.5% in 2nd decade, 69.2% in 3rd decade, 78.3% in 4th decade and 91. 7% in above 5th decade. 4. HBeAg positive rate decreased stepwise with age, while anti-HBe positive rate increased stepwise with age. 5. ALT abnormal cases in the HBeAg positive donors were higher than normal cases, but ALT normal cases in the anit-HBe positive donors were higher than abnormal cases.

HBA 양성환자의 혈청 Type Ⅳ Collagen 측정치 비교

윤기은,문희주,박정오 서울保健大學 1996 論文集 Vol.16 No.1

The purpose of this study was to analyze the concentration of serum type w Ⅳcollagen in HBV-DNA positive patients. The results were as follows. 1. As HBV-DNA concentration of serum grew higher, that of serum type Ⅳcollagen became lower gradually. 2. Total LDH activity had a significant relationship with concentration of serum type Ⅳcollagen up to l,000ng/mL. As HBV-DNA concentration of serum grew higher, total LDH activity became lower gradually. 3. Correlation serum type N collagen with AST(GOT) and ALT(GPT) appeared to have little relation compared to LDH. But each generally had the same change in direction. As HBV-DNA concentration of serum grew higher, those of AST and ALT were decreased drastically. These were no differences between the test group and a normal group.

혈뇨의 감별진단을 위한 이형적혈구의 형태학적 연구

윤철종,박정오,문희주,윤기은 韓國電子顯微鏡學會 1999 Applied microscopy Vol.29 No.3

전체 성인의 약 4%에서 정도 차이는 있지만 혈뇨가 있다고 볼 때 그 원인을 구명하는 일이 중요하다. 그 원인이 사구체성 신염인지 기타 그 외의 비사구체성 원인에 따른 질환인지를 쉽게 감별하는 일이 중요하다. 본 연구자들은 혈뇨를 동반한 소아과 환자중에서 신 생검 조직검사까지 한 5명의 환자에서 혈뇨를 표본 제작하여 먼저 위상차현미경적 표본제작법을 통하여 적혈구를 감별하였고 방법을 달리하여 주사형 전자현미경으로 요침사를 관찰하여 형태학적 고찰을 통하여 이형적혈구를 보다 명확한 3차원구조를 관찰할 수 있었다. 침생검을 통해 얻은 조직학적 소견으로는 사구체의 기저막을 통과한 혈구들의 요로를 통하여 배출과정을 광학현미경 및 투과형 전자현미경으로 확인할 수 있었다. 본 연구에서 육안적 혈뇨의 색은 유출되는 적혈구의 양과 상관관계가 있으며 용혈된 정도가 심한 경우에 콜라색 혈뇨가 있음을 알 수 있었다. 이들 결과에서 본 연구자들은 사구체성 질환을 동반한 유극적혈구 및 환형 적혈구 등과 같은 변형된 적혈구를 주사형 전자현미경으로 명확한 관찰을 함으로 사구체성 질환으로 인한 혈뇨의 감별에 주사형 전자현미경이 가장 유용한 검사 방법이라고 생각된다. Examination of the morphology of red blood cells in the urine has been shown to be a promising adjunct in determining whether hematuria represents glomerular or nonglomerular bleeding. This is due to distortion of RBCs as they pass across the basement membrane of the glomerular capillaries. It is concluded that is method can greatly help the clinician in distinguishing between glomerular and non glomerular bleeding in patients with hematuria and channeling such patients toward the most appropriate investigations. We have experimented dysmorphic red blood cells that 5 patients of the hematuria are distorted with irregular outlines and often have small blebs extruding from the red cell membrane. Tried urinary sediments were seen with phase contrast microscope and confirmed scanning electron microscope. There are seen acanthocytes, anulocytes, ghost cells and sphero-echinocytes in dysmorphic erythrocytes. Clinical diagnosis was referred from the result of the biopsy-proven. Scanning electron microscopic findings of the hematuria are good diagnostic tool that disclose in distorted red blood cells from patients with glomerular disorders.

供血者에서의 HBsAg 發現率과 生化學的 檢査値에 관한 硏究

문희주,윤기은,김승곤,라동진,김태전,박정오,최범열 서울保健大學 1988 論文集 Vol.8 No.1

고카라트 Au-Zn-Pt 합금의 등온시효경화 거동

이희경,박영환,문희만,이정은,김형일 大韓齒科器材學會 2001 대한치과재료학회지 Vol.28 No.4

The isothermal age-hardening behavior of a high carat Au-Zn-Pt alloy was investigated by means of hardness test, X-ray diffraction study, microscopic observation and electron probe micro analysis. By the isothermal ageing of the solution-treated specimen at 300∼400℃, the hardness increased slowly at the initial stage of ageing and reached a maximum hardness values at 300∼400℃ was similar and suitable to use as the crown & bridge. By the isothermal ageing of the solution-treated specimen at 300∼400℃, three phases of the Au-rich α_1 phase with face-centered cubic structure,the Pt_3Zn a_2 phase with an ordered AuCu_(L1_2) type(f.c.c) and the Pt-rich a_3 phase with face-centered cubic structure were transformed into three phases of the α_1 phase, the α_3 phase and the PtZn β phase with an ordered AuCu I(L1_0) type. The hardening seemed to be attributed to the lattice strains of the matrix resulting from the transformation of the a_2 phase to the β phase. The overageing with softening was attributed to the coarsening of the nodule precipitates which seemed to be composed of the β phase and α_1 matrix.

尿中 Aminolevulinic Acid와 Porphobilinogen의 비교 조사 연구

나동진,윤기은,김승곤,문희주,김태전,박정오 서울保健大學 1990 論文集 Vol.10 No.1

This studies were examined to measure total ALA and PBG concentration in Urine, and find the relationship of correlation among them in 57 men unexposed and 43 men exposed by mercury. The results were summarized as the following; 1. In 100 populations total urine ALA concentration was 0.85±0.17㎎% in male, in female were 0.40 ± 0.04㎎% (normal value : 0.54㎎%). 2. Urine ALA concentration in male were 1.04∼1.11mg% for the ages between 30 ∼ 49 was the highest and then in female were 0.58㎎% for the ages between 40 - 59 was the highest. 3. In 100 populations total urine PBG concentration was 0.03㎎% in male, in female were 0.02㎎% (normal value : 0.03㎎%). 4. Urine PBG concentration in male were 0.05㎎% for the ages between 30-49 was the highest. 5. In unexposed men group total urine ALA concentration was 0.13±0.03㎎% in male, in female were 0.11±0.0l㎎%. 6. In exposed men group total urine ALA concentration was 1.81±0.55㎎% in male, ill female were 0.78±0.09㎎%, there was differences in attention showing male was higher than the female (P<0.05). 7. In unexposed men group total urine PBG concentration was 0.02㎎% in male, in female were 0.0l㎎%. 8. In exposed men group total urine PBG concentration was 0.06㎎% in male, in female were 0.04㎎%,there was differences in attention showing male was higher than the female (P<0.05).

병원내 생리기능 검사실의 적정 업무환경에 관한 연구

배형준,윤기은,김승곤,나동진,문희주,김태전,박정오 서울보건대학 1999 서울보건대학 부설 병원경영연구소 논문집 Vol.4 No.2

The purpose of this study was to suggest guideline for optimal work environment of physiological laboratory in hospital. Therefore this study was carried out to analyze the actual condition of laboratory system, major equipments and persons. The period of survey was from September to November 1997 and the objects were 123 laboratory. The major results of this study were as follows. 1. The optimal areas of general ECG room, exercise ECG room and echocardiology room were all 9-18㎡, that of Holter ECG room was 3-12㎡. The optimal areas of neurosystem laboratory were all 9-12㎡, those of respiratory system laboratory were all 9-18㎡. 2. The optimal number of persons laboratory of hospital 500 beds and less were 2∼3, those of 501∼700 beds were 3∼4.701∼1.000 beds were 4∼5 and over 1.001 beds were 7∼8 persons in circulatory system laboratory. The optimal number of persons laboratory of hospital 500 beds less than were 1∼2, those of 501∼700 beds were 3∼4, 701∼1.000 beds were 6∼7 and over 1.001 beds were over 8 persons in neurosystem laboratory. The optimal number of persons laboratory of hospital 500 beds and less 1. those 501∼1.000 beds were 1∼2 and over 1.001 beds were 2∼3 persons in repiratory system laboratory. 3. The optimal number of equipments of general ECG were 2∼3 in the 500∼1.000 beds hospital. there were 3∼4 units in the 1,001 beds. The optimal number of equipment EEG were both 1∼2 in the hospital 500 beds and less and 501∼700 beds. those were 4∼5 units in hospital over 701 beds. The optimal number of equipments of spirograph were both 1∼2 in the hospital 500 and less and 501∼1.000 beds. those were 2∼3 units in the over 1.001 beds. 4. In view of speciality and legal problem of physiofunctional test. we sugget that non-licensee were so quickly change place licensee in the interests of optimal work environment. 5. We suggest that to upgrade qualitative level of domestic equipment and to the more amplify utilization with respect to domestic equipment for the purpose of reducing loss of foreign money.