국내 자생 민들레의 종류별 항산화성 및 세포독성 연구

천상욱 한국작물학회 2012 한국작물학회지 Vol.57 No.1

민들레의 종류별 성분 및 생리활성 차이를 검토하고자 폴리페놀 함량, 플라보노이드 함량, 항산화성 및 항암성을 분석하였다. 민들레 종류별 1000mgkg1의 메탄올 추출물의 총 페놀 함량은 지상부 추출물이 50.2~76.8mgkg1범위로 지하부 추출물 24.9~40.0mgkg1범위보다 높게 나타났으며, 종별로는 민들레가 가장 높았고, 그 다음으로 흰민들레와 서양민들레 순으로 나타났다. 민들레의 지상부 및 지하부 추출물에서 각각 76.8과 40.0mgkg1를 보여 유의적으로 가장 높은 함량을 보였다(p < 0.05). 한편, 총 플라보노이드 함량은 총 페놀 함량과 유사한 경향을 보였으나 총 페놀 함량보다 낮은 함량 범위(6.5~36.4mgkg1)를 보였다. DPPH 라디컬 소거능은 추출물 농도가 증가할수록 높은 활성을 보였으며 민들레의 지상부와 뿌리 추출물 1,000mgkg1에서 DPPH 라디컬 소거능은 각각 89.6와 83.4%의 소거능을 보여 다른 두 종보다 높은 활성을 보였다. MTT법에 의한 세포독성 시험에서 민들레 종류별 지상부와 지하부 추출물의 폐암세포주(Calu-6)에 대한 세포 생존율은 민들레 추출물에서 가장 낮았고(IC50값 = 83.4과 66.4mgkg1), 그 다음으로 흰민들레와 서양민들레 순으로 높은 생존율을 보여 민들레가 다른 두 종에 비해 높은 세포독성이 있음을 보여 주었다. 한편, 위암세포주(SNU-601)에 대한 세포 생존율은 폐암세포주에 비해 상대적으로 높은 경향으로 이는 추출물이 더 낮은 항암활성을 갖고 있음을 나타냈다. 각 성분과 생리활성 항목간의 상관관계에 있어서 총 페놀 함량과 항산화 활성 또는 세포독성 항목간의 상관관계(r2 =0.0097~0.6213)는 총 플라보노이드 함량과 항산화 활성 또는 세포독성 항목간 상관관계(r2 =0.0027~0.4627) 보다 높게 나타났다. 결과적으로 총 페놀 함량과 총 플라보노이드 함량이 항산화성 및 세포독성 과 높은 연관성을 보이며, 그 함량과 활성은 민들레 종류에 따라 다르게 나타났다. Contents of phenolics and flavonoids, antioxidant activity and cytotoxicity were investigated in the methanol extracts of three different Taraxacum species, Taraxacum coreanum , Taraxacum mongolicum , and Taraxacum officinale . Total phenolics content at 1000mg~;kg-1 was more present in shoot parts than in roots, and was highest in T. mongolicum shoot and root extracts (76.8 and 40.0mg~;kg-1 , respectively), followed by T. coreanum and T. officinale (p < 0.05). Total flavonoid level had same tendency to total phenolics among Taraxacum species, showing lower amounts (6.5~36.4mg~;kg-1 ) than total phenolics. The antioxidant activity of the methanol extracts from all the species dose-dependently increased. DPPH free radical scavenging activity at 1,000mg~;kg-1 was highest in shoot and root extracts from T. mongolicum by 89.6 and 83.4%, respectively. According to MTT assay, cell viability of Calu-6 (human pulmonary carcinoma) was lowest in the T. mongolicum shoot and root extracts (IC50 values=83.4 and 66.4mg~;kg-1 , respectively), and followed by T. coreanum and T. officinale (lowest). Calu-6 was more sensitive to the extracts than SNU-601 (human gastric carcinoma). Antioxidative and anticancer activities in three different Taraxacum species was more correlated with total phenolics content (r2 =0.0097 to 0.6213) than with total flavonoids level (r2 =0.0027 to 0.4627). The results showed total phenolics content and total flavonoids level were highly correlated with anticancer activity and antioxidant activity, and their content and activities were different depending on species.

Mechanisms of Hela Cell Apoptosis Induced by Abnormal Savda Munziq Total Phenolics Combined with Chemotherapeutic Agents

Zhang, Yun-Xia,Abliz, Guzalnur,Ye, Wei-Jun,Mutalipu, Zuohelaguli,Li, Xiao-Wen,Wang, Hai-Qin,Buranjiang, Gulimire,Upur, Halmurat Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Objective: To investigate the effects of abnormal Savda Munziq (ASMq) total phenolics combined with cisplatin and docetaxel on the Hela cell growth. Methods: In vivo cultured Hela cells were treated with cisplatin, docetaxel, total phenolics, cisplatin+total phenolics or docetaxel+total phenolics. MTT was performed to assess inhibition of cell proliferation, flow cytometry to detect apoptosis, and semi-quantitative RT-PCR to test for survivin and Bcl-2 expression. Results: The total phenolics, cisplatin and docetaxel had significant inhibitory and apoptosis-promoting effects on Hela cells (P<0.05), with the early apoptotic rates of $12.8{\pm}0.70%$, $18.9{\pm}3.79%$ and $15.8{\pm}3.8%$; the total phenolics, cisplatin and docetaxel significantly decreased the expression of Bcl-2 and survivin (all P<0.01), especially when used in combination. Conclusion: ASMq total phenolics, combined with cisplatin and docetaxel, could promote the apoptosis of Hela cells possibly through reducing the expression of Bcl-2 and survivin.

Optimization of shoot cultures and bioactive compound accumulation in Rosa rugosa during acclimatization

Jang, Hae-Rim,Park, Byung-Jun,Park, Seung-A,Pee, Ok-Ja,Park, So-Young,Paek, Kee-Yoeup The Korean Society of Plant Biotechnology 2016 식물생명공학회지 Vol.43 No.1

Rosa rugosa is a medicinal, ornamental, and edible plant native to Eastern Asian countries, including Korea, Japan, and China. The aim of this study was to establish a system for biomass production and secondary metabolite accumulation during in vitro culture and acclimatization of Rosa rugosa. The highest rate of multiple shoot proliferation was achieved with $8.8{\mu}M$ benzyladenine (BA) (83.3%). However, the number of shoots (14.4 per explant) at $4.4{\mu}M$ BA was higher than that at $8.8{\mu}M$ BA. Compared to BA, a combination of thidiazuron (TDZ) and indole butyric acid (IBA) exhibited significantly lower shoot induction, with only 50.0~79.2% and 4.2~16.7% relative shoot formation, respectively. During acclimatization, shoots were sampled every week and their total phenolic contents were analyzed. Among various growth factors, fresh weight showed the most dramatic increase from the 3rd week (88.0 mg/plant) to 4th week (132.7 mg/plant). Total phenolics and flavonoids contents were the highest at $1^{st}$ week of acclimatization. Depending on developmental stages, total phenolics and flavonoids contents were higher in 1-yr-old shoots grown ex vitro than in those of older field-grown or in vitro-grown plants. Amongst different ages of field grown plants, 6-year-old plants, the oldest in this study, showed the lowest content in total phenolics.

Optimization of shoot cultures and bioactive compound accumulation in Rosa rugosa during acclimatization

장해림,박병준,박승아,피옥자,박소영,백기엽 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.1

Rosa rugosa is a medicinal, ornamental, and edible plant native to Eastern Asian countries, including Korea, Japan, and China. The aim of this study was to establish a system for biomass production and secondary metabolite accumulation during in vitro culture and acclimatization of Rosa rugosa. The highest rate of multiple shoot proliferation was achieved with 8.8 μM benzyladenine (BA) (83.3%). However, the number of shoots (14.4 per explant) at 4.4 μM BA was higher than that at 8.8 μM BA. Compared to BA, a combination of thidiazuron (TDZ) and indole butyric acid (IBA) exhibited significantly lower shoot induction, with only 50.0 ~ 79.2% and 4.2 ~ 16.7% relative shoot formation, respectively. During acclimatization, shoots were sampled every week and their total phenolic contents were analyzed. Among various growth factors, fresh weight showed the most dramatic increase from the 3rd week (88.0 mg/plant) to 4th week (132.7 mg/plant). Total phenolics and flavonoids contents were the highest at 1 st week of acclimatization. Depending on developmental stages, total phenolics and flavonoids contents were higher in 1-yr-old shoots grown ex vitro than in those of older field-grown or in vitro-grown plants. Amongst different ages of field grown plants, 6-year-old plants, the oldest in this study, showed the lowest content in total phenolics.

국내산 프로폴리스의 총플라보노이드 및 총페놀함량 비교

우순옥(Woo, Soon Ok),홍인표(In-Pyo Hong),한상미(Sangmi Han) 한국양봉학회 2015 韓國養蜂學會誌 Vol.30 No.4

Propolis is a sticky material like resin made from plant growing point which are collected by bees, then mixed with bee saliva enzyme. It is used to keep bee colony safe by applying inside of bee hive. The propolis, it is necessary to extract the active ingredient in raw material, mainly extracted by the ethyl alcohol. Ethanol extracted propolis collected from different regions in Korea, we compared the total flavonoids and total phenolic contents. The average of total flavonoids content of propolis were 6.1% in central regions, 5.7% in southern regions, however, propolis in Jeju island represented the average value of 0.44%. The other areas showed a value of 7.4%. The total phenolic contents of propolis were 21.8% in the central region, 20.9% in southern regions, 13.7% in Jeju, and the other areas showed a value of 29.4%. Although some differences depending on the region, the propolis produced in Korea has been as the substance that is rich in flavonoids and phenolics.

한국산 약용식물로부터의 SOD활성 및 Phenolic Compounds 함량 비교

임정대,유창연,김명조,윤성중,이선주,김나영,정일민 한국약용작물학회 2004 한국약용작물학회지 Vol.12 No.3

한국에 분포하고 있는 82종의 약용식물에 대하여 환경내성 및 기능성 소재의 개발을 위한 탐색을 위하여 항산화효소와 관련된 첫 번째 방어기작인 SOD 효소활성과 Phenol 화합물의 함량정도를 검정하여 본 결과 SOD 효소활성은 원지 (P. tenuifolia Willd.), 백편두 (D. lablab L.), 백개자 (S. alba L.), 대황 (R. palmatum L.), 감초 (G. uralensis Fisch), 연자육 (N. nucifera Gaertn), 지황 [R. glutinosa (Gaertner) Liboshitz], 현삼 (S. buergeriana Miq.), 인진 (A. capillaris Thb.)등에서 다른 약용작물보다 비교적 높은 SOD 효소 활성 능력을 나타냈다. Total phenol함량은 (3~249.731 μg/g)의 범위를 보였으며 지유(S. officinalis L.), 대황 (R. palmatum L.), 복분자 (R. chingii Hu)에서 각각 249.731 μg/g, 209.546 μg/g, 170.333 μg/g으로 가장 높은 함량을 나타냈다. 16종의 phenol 화합물의 경우에서는 82종의 약용식물이 각기 매우 상이한 농도의 분포를 나타내었다. 앞으로의 연구에서는, 항산화 효소 및 phenol 화합물이 많이 함유하여 우수한 기능성을 가지고 있는 것을 확인된 약용식물에 대해 그들의 이용성을 증대시킬 수 있는 구체적인 연구가 수행되어져야 할 것으로 사료된다. 또한 phenol성 물질을 많이 함유하는 약용식물 추출물을 이용한 기능성 식품, 화장품, 천연 보존재 및 의약품 등으로 개발 (Cook & Samman, 1996)할 수 있을 것으로 판단되어진다. 82 species of Korean medicinal plants were measured to their SOD (superoxide dismutase) activity, total phenol and individual phenol compounds. The SOD activity of medicinal plants was varied from 3.67% to 48.3%, and especially, 4 species including Polygala tenuifolia Willd. showed activity over 40% higher than other species. The 16 individual phenol compounds concentrations have a severe variation in medicinal plants. Codonopsis lanceolata is the highest concentration (12,176 μg/g), and 10 species including Glycyrrhiza uralensis Fisch were showed to concentrations more than 1,000 μg/g. And also, salicylic acid was generally found in many plants, but trans-cinnamic acid, naringenin and kampferol was little found in plants. The total phenol by Folin-Dennis methods showed a respectively variation (3~249.731 μg/g) in 82 medicinal plants, and 7 species including Astragalus membranaceus showed low total phenol concentrations less than 10 μg/g. In conclusion, we expect that selecting medicinal plants with high biological activity might develop to chemicals related to antioxidants, as well as these become most popular substance at many industrial materials, and suggest to more researchs in future.

Rapid Preparation of Total Nucleic Acids from E. coli for Multi-purpose Applications

Cheng, Lin,Li, Tai-Yuan,Zhang, Yi Korean Society for Biochemistry and Molecular Biol 2004 Journal of biochemistry and molecular biology Vol.37 No.3

Separate protocols are commonly used to prepare plasmid DNA, chromosomal DNA, or total RNA from E. coli cells. Various methods for the rapid preparation of plasmid DNA have been developed previously, but the preparation of the chromosomal DNA and total RNA are usually laborious. We report here a simple, fast, reliable, and cost-effective method to extract total nucleic acids from E. coli by direct lysis of the cells with phenol. Five distinct and sharp bands, which correspond to chromosomal DNA, plasmid DNA, 23S rRNA, 16S rRNA, and a mixture of small RNA, were observed when analyzing the prepared total nucleic acids on a regular 1-2% agarose gel. The simple and high-quality preparation of the total nucleic acids in a singe tube allowed us to rapidly screen the recombinant plasmid, as well as to simultaneously monitor the change of the plasmid copy number and rRNA levels during the growth of E. coli in the liquid medium.

커피박 추출물의 총 페놀화합물 함량 및 유리라디칼 소거능

김혜인(Kim, Hye-In),이지영(Lee, Ji-Young),배진영(Bae, Jin-Young),양선임(Yang, Sun-Im),김혜진(Kim, Hye-Jin),인만진(In, Man-Jin),김동청(Kim, Dong-Chung) 한국외식산업경영학회 2013 외식산업경영연구 Vol.9 No.1

커피박의 항산화능을 확인하기 위하여 커피박 추출물의 고형분, 갈색 물질 및 총 페놀화합물 함량을 측정하였고, DPPH 유리라디칼 소거능을 확인하였다. 커피박의 추출용매로는 70% 에탄올을 사용하였다. 커피박 추출액에는 2.86%(w/w) 의 고형분이 함유되어 있고, 상당량의 갈색물질이 잔존함을 확인하였다. 또한 커피박 추출액에는 303.54㎍/ml의 페놀성 화합물이 있었으며, DPPH 유리라디칼 소거능을 가지고 있었다. 커피박 추출물의 유리라디칼 소거능은 그 추출물에 함유되어 있는 갈색 물질 및 총 페놀화합물 함량과 상관관계를 가지고 있었다. 본 연구는 커피산업의 부산물인 커피박의 천연 항산화 소재로서의 활용 가능성을 보여 주었다. To determine the antioxidant potential of roasted ground coffee residue, we investigated the soluble solid content, brown color intensity, total phenolics and free radical scavenging activity of roasted ground coffee residue extract. The roasted ground coffee residue extract was prepared using 70% ethanol as an extraction solvent. The extract contained 2.86%(w/w) of soluble solid and showed a relatively high brown color intensity. Also, the extract contained 303.54 ㎍/ml of total phenolics and possessed free radical scavenging activity. The free radical scavenging activity showed a positive correlation with brown color intensity and total phenolics. It is suggested that roasted ground coffee residue could be considered as a natural antioxidant substance.

Rapid Preparation of Total Nucleic Acids from E. coli for Multi-purpose Applications

( Lin Cheng ),( Tai Yuan Li ),( Yi Zhang ) 생화학분자생물학회 2004 BMB Reports Vol.37 No.3

Separate protocols are commonly used to prepare plasmid DNA, chromosomal DNA, or total RNA from E. coli cells. Various methods for the rapid preparation of plasmid DNA have been developed previously, but the preparation of the chromosomal DNA and total RNA are usually laborious. We report here a simple, fast, reliable, and cost-effective method to extract total nucleic acids from E. coli by direct lysis of the cells with phenol. Five distinct and sharp bands, which correspond to chromosomal DNA, plasmid DNA, 23S rRNA, 16S rRNA, and a mixture of small RNA, were observed when analyzing the prepared total nucleic acids on a regular 1-2% agarose gel. The simple and high-quality preparation of the total nucleic acids in a singe tube allowed us to rapidly screen the recombinant plasmid, as well as to simultaneously monitor the change of the plasmid copy number and rRNA levels during the growth of E. coli in the liquid medium.

당귀 잎의 항산화 활성

박성진 ( Sung Jin Park ),윤정한 ( Jung Han Yoon ),김영언 ( Young Eon Kim ),윤원병 ( Won Byong Yoon ),김종대 ( Jong Dai Kim ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.6

Angelicae gigas Nakai has been used as a traditional medicine as well as an edible vegetable in South Korea. In this study, the total phenolic and flavonoid content and antioxidants of A. gigas Nakai leaves were examined in vitro via hydroxyl-radical-scavenging activity, reducing power activity, metal chelating assay, and DPPH-free -radical-scavenging assay. Among all the extracts from A. gigas Nakai leaves, the ethanol extract showed the strongest effects in all of the assays. The EC50 values for the DPPH-radical-scavenging activities of ethanol, methanol, and water extracts were 31.47, 42.14, and 58.47 μg/mL, respectively. Among the extracts from A. gigas Kakai leaves, the ethanol extract had the highest levels of total phenolics (7.84 ± 1.46 mg TAN/g) and total flavonoids (4.23 ± 0.03 mg QE/g), which correlated strongly with the individual phenolic-compound (p-hydroxybenzoic acid, vanillin, and trans-ferulic acid) contents. The ethanol extract also showed stronger antioxidant activity than tocopherol in hydroxyl- radical-scavenging activity assay. These results indicate that the ethanol extract of A. gigas Kakai leaves possesses significant antioxidant properties, which suggests its great potential as a functional-food ingredient in the food industry.