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정일민,이택준,오용택,Bimal Kumar Ghimire,장인배,김승현 고려인삼학회 2017 Journal of Ginseng Research Vol.41 No.2
Background: The natural ratios of carbon (C), nitrogen (N), and sulfur (S) stable isotopes can be varied in some specific living organisms owing to various isotopic fractionation processes in nature. Therefore, the analysis of C, N, and S stable isotope ratios in ginseng can provide a feasible method for determining ginseng authenticity depending on the cultivation land and type of fertilizer. Methods: C, N, and S stable isotope composition in 6-yr-old ginseng roots (Jagyeongjong variety) was measured by isotope ratio mass spectrometry. Results: The type of cultivation land and organic fertilizers affected the C, N, and S stable isotope ratio in ginseng (p < 0.05). The d15NAIR and d34SVCDT values in ginseng roots more significantly discriminated the cultivation land and type of organic fertilizers in ginseng cultivation than the d13CVPDB value. The combination of d13CVPDB, d15NAIR, or d34SVCDT in ginseng, except the combination d13CVPDBe34SVCDT, showed a better discrimination depending on soil type or fertilizer type. Conclusion: This case study provides preliminary results about the variation of C, N, and S isotope composition in ginseng according to the cultivation soil type and organic fertilizer type. Hence, our findings are potentially applicable to evaluate ginseng authenticity depending on cultivation conditions.
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정일민,김광호,안종국 韓國藥用作物學會 1998 韓國藥用作物學會誌 Vol.6 No.4
60종(種)의 자생 약용식물과 식용작물을 대상으로 항산화활성의 생리활성을 조사함으로써 약용식물과 식용작물의 유용성 측면의 확인뿐만 아니라 새로운 생리활성물질 탐색의 가능성을 검토하기 위해서 실시한 실험 결과는 다음과 같다. 자생 약용식물 및 식용작물에 대한 50% EtOH 추출물을 이용하여 TBA법. DPPH법에 의하여 1차 활성검정 결과 처리방법에 따라 활성값에 차이가 있으나 TBA법에서는 검정콩의 추출액이 87.3%, DPPH법에서는 까마중 추출액이 80.6%로서 가장 높은 활성을 보였고 두가지 방법에서 동시에 활성이 높은 것은 검정콩을 비롯하여 10종 이었다. SOD활성정도도 검정식물에 따라 큰 차이가 있으나 검정콩이 53.5%로서 가장 높은 SOD활성을 나타내었으며 SOD종류는 Cu/ZnSOD, FeSOD을 포함하는 것으로 나타났다. Cu/ZnSOD만을 함유하고 있는 잔대는 SOD활성 정도가 10.37%로서 실험 대상식물 중 가장 낮은 활성을 보였다. Sixty medicinal and food plants native to Korea were mainly selected with old traditional habit and antioxidant activity was investigated. The 80% EtOH extracts of sixty medicinal and food plants were screened for antioxidant activity. Antioxidant activity was measured by the TBA (Thiobarbituric acid), DPPH (1, 1-Diphenyl-2-picrylhydrazyl), SOD (superoxide dismutase) which was evaluated by the nitro blue tetrazolium(NBT) reduction method. Among sixty plants, black Glycine max(87. 3%) and Solanum nigrum (80.6%) exhibited the highest antioxidant activity by TBA and DPPH methods, respectively. Also, 10 species extracts including black Glycine max showed the high activity value in these two methods. The SOD characteristics on black Glycine max seed extracts which showed the highest SOD activity (53.5%) exhibited four major SODs; two Cu/ZnSODs and two FeSODs. However, Adenophaora vertidllata which showed lowest SOD value (10.4%) had only Cu/Zn SOD. No varietal differences in the high SOD value were detected in the Cu/Zn SOD isozyme patterns.
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정일민,김광호,안종국,안종석,안순철 韓國藥用作物學會 1999 韓國藥用作物學會誌 Vol.7 No.1
60종(種)의 약용식물과 식용작물을 대상으로 항종양활성등의 생리활성을 조사함으로서 약용식물과 식용작물의 유용적인 측면의 확인뿐만 아니라 나아가서 새로운 생리활성물질 탐색의 가능성을 검토하기 위해서 실험하였으며 결과는 다음과 같다. 약용식물 및 식용작물에 대한 80% EtOH 추출물을 이용하여 항종양효과를 보면 PKC법에서는 명아주(73.4%) 및 antibleb형성억제력검정에서는 검정콩이, PLC법에서는 검정콩(91.9%), MTT법에서 50%의 억제력을 나타내는 농도(IC50)가 검정콩과 쑥의 경우 각각 4.7μg/ml을 보였다. This study was conducted to screen antitumoral activity by in vitro bioassay method using 60 Korean medicinal and food plants extracted by 80% EtOH. Antitumor activity test was applied by the PKC(protein kinase C) and antibleb formation, PLC (Phospholipase C), and colorimetric tetrazolium assay (MTT assay) methods. Chenopodjum album and black Glycine max showed high antitumoral activity by 73.5% and 81.0%, respectively, against PKC by bleb-forming assay and PKC enzyme assay on human chronic leukemia K562 cell. Black Glycine max also showed 91.2% antitumoral activity in the PLC method and the lowest IC50 value(4.7μg/ml) by MTT method against P-338 cell line. In the effect of the concentration treatment on antitumoral test, the more concentration indicated the more activity value.
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