한국인에서의 전염성연속종 바이러스의 분자생물학적 역학에 관한 연구

최지호(Jee Ho Choi),김규한(Kyu Han Kim),김성범(Seong Beom Kim),서정화(Jung Wha Suh),고재경(Jai Kyoung Koh),성경제(Kyung Jeh Sung) 대한피부과학회 1994 大韓皮膚科學會誌 Vol.32 No.5

Background : Recent restriction enclonuclease analysis studies hsve revealed that MCV DNA can be classified into two major types, designated MCV-1 and MCV-2, by th:ir restriction enzyme cleavsge patterns. In earlier reports of MCV DNA analysis, MCV-2 was the main virus type found in genital lesions. However many recent studies cienied the relationship between virus type and anatomical distribution. Objective : The purpose of this study was to examine the ratio of MCV-l to MCV-2 in Korean isolates of MCV DNA and the relationship between MCV subtypes and with clinical features such as anatomical location, age, sex, numiber of lesions, and atopic dermatitis. Methads : MCV DNA extrated from 112 cases of Korean patients waa examined by restriction endonuclease analysis using Brtm HI. Results : 1. MCV-1 was found in 108 of 112 (96.4%) patients and MCV-2 in of 112 (3.6%) patients. The ratio of MCV-1 to MCV-2 wss 28:1. 2. There was no significant ciprrelation between MCV subtypes and the age, sex, number of lesions, atopic dermatitis, and anatoimic loction. 3. Lesions induced by MCV-1 MCV-2 were indistinguishable on the brsis of size and form. Conclueion : This study showis that the ratio of MCV-1 to MCV-2 was 28:1 in Korean molluscum contagiosum patients and there was no relationship between MCV subtyies and lesional morphology or snatomical distribution. (Kor J Dermatol 1994;32(5):763-769)

철결핍성빈혈 진단과 RDW, MCV

조경진(Kyung-Jin Cho),남영미(Young-Mi Nam),강연주(Yon-Ju Kang),민해연(Hae-Yon Min) 대한의생명과학회 1995 Biomedical Science Letters Vol.1 No.1

자동혈구계산기에서 산출되는 RDW나 MCV가 철결핍성빈혈의 구분진단에 어떠한 도움이 되는지를 보기 위하여 어느 대학병원의 최근 5년 동안의 의무기록과 건강진단결과를 이용하여 227명의 빈혈환자와 143명의 건강인을 선정하고 그 들의 혈액검사 결과를 비교분석해 보았다. 분석결과 빈혈환자로서는 철결핍성빈혈과 재생불량성빈혈 환자가 많았으며, 그 외에 만성질환과 관련된 빈혈환자도 많은 것으로 나타났다. 정상인 들에 비하여 빈혈환자에서 RDW는 높게, 그리고 MCV는 낮게 나타났는데 특히 철결핍성빈혈에서는 다른 빈혈에 비하여 19.3±4.8로 현저하게 높게 나타난 반면, MCV는 62.9±13.7fl로 현저하게 낮게 나타났으며, 그 빈혈의 정도에 따라 그 변화가 크게 나타났다. 빈혈관련수들을 이용하여 D.F.=0.26-0.012MCV-0.130MCH+0.073MCHC+0.052RDW+0.003PLT와 같은 판별함수가 도출되었고, 이를 토대로 철결핍성빈혈과 다른 빈혈을 구분하는데 있어서는 MCV, MCH, MCHC, RDW등이 판별력이 있다는 것을 확인할 수 있었다. RDW and MCV are thought to be the highly sensitive blood cell parameters in the differentiation of iron deficiency anemias. Through the medical records of 227 anemic patients and the physical checking results of 143 healthy persons in a General Hospital during the recent five years, the authors evaluated various blood cell parameters including RDW and MCV.Iron deficiency anemia, aplastic anemia and other anemias associated with chronic disease were shown as the three major causes of anemias in Korea. In the patients of iron deficiency anemia MCV was very low(62.9±13.7fl), while RDW was very high(19.3±4.8) showing much lower MCV and much higher RDW in severe IDA compared with in mild IDA. To differentiate iron deficiency anemias form other anemias, a discriminant function was developed from some blood cell parameters like MCV, MCH, MCHC, RDW and platelets(D.F.=0.26-0.012MCV-0.130MCH+0.073MCHC+0.052RDW+0.003PLT).

철결핍성빈혈 진단과 RDW, MCV

민해연,강연주,조경진,남영미 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCINE 1995 Journal of biomedical laboratory sciences Vol.1 No.1

자동혈구계산기에서 산출되는 RDW나 MCV가 철결핍성빈혈의 구분진단에 어떠한 도움이 되는지를 보기 위하여 어는 대학병원의 최근 5년 동안의 의무기록과 건강진단결과를 이용하여 227명의 빈혈환자와 143명의 건강인을 선정하고 그들의 혈액검사 결과를 비교분석해 보았다. 분석결과 빈혈환자로서는 철결핍성빈혈과 재생불량성빈혈 환자가 많았으며, 그 외에 만성질환과 관련된 빈혈환자도 많은 것으로 나타났다. 정상인 들에 비하여 빈혈환자에서 RDW는 높게, 그리고 MCV는 낮게 나타났는데 특히 철겹핍성빈혈에서는 다른 빈혈에 비하여 19.3±4.8로 현저하게 높게 나타난 반면, MCV는 62.9±13.7fl로 현저하게 낮게 나타났으며, 그 빈혈의 정도에 따라 그 변화가 크게 나타났다. 빈혈관련수들을 이용하여 D.F.=0.26-0.012MCV-0.130MCH+0.073MCHC+0.052RDW+0.003PLT와 같은 판별함수가 도출되었고, 이를 토대로 철결핍성빈혈과 다른 빈혈을 구분하는데 있어서는 MCV, MCH, MCHC, RDW등이 판별력이 있다는 것을 확인 할수 있었다. RDW and MCV are thought to be the highly sensitive blood cell parameters in the differentiation of iron deficiency anemias. Through the medical records of 227 anemic patients and the physical checking results of 143 healthy persons in a General Hospital during the recent five years, the authors evaluated various blood cell parameters including RDW and MCV. Iron deficiency anemia, aplastic anemia and other anemias associated with chronic discase were shown as the three major causes of anemias in Korea. In the patients of iron deficiency anemia MCV was very low(62.9±13.7fL), while RDW was very high(19.3±4.8) showing much lower MCV and much higher RDW in severe IDA compared with in mild IDA. To differentiate iron deficiency anemias form other anemias, a discriminant function was developed from some blood cell parameters like MCV, MCH, MCHC, RDW and platelets(D.F.=0.26 -0.012MCV -0.130MCH+ 0.073MCHC +0.052RDW +0.003PLT).

철결핍성 빈혈환자의 내원시 검사 소견

강한걸,신석균,최소연,김현수,송영구,서형찬,김효철 아주대학교 1996 아주의학 Vol.1 No.2

저자들은 철결핍성 빈혈 환자들의 내원시 검사소견을 관찰하여 이들의 특성 및 진단에 유용한 지표를 찾고 아울러 혈청 내 철의 정도를 반영할 수 있는 적혈구지수가 있는 지 알아보고자 1994년 1월부터 1996년 1월까지 아주대학교 부속대학병원 혈액종양내과에 내원하여 Coulter STK A2를 통해서 얻은 적혈구지수와 혈청 철, 혈청 총철결합능력, 혈청 ferritin을 측정하여 진단된 IDA환자 130례를 대상으로 다음과 같은 결과를 얻었다. 1) 전체 환자의 평균연령은 36.3±11.6세였으며 12세에서 65세까지의 연령분포를 보였다. 2) 전체 환자의 혈색소는 8.0±2.0 g/㎗이었고, 헤마토크리트 26.0±5.4%, MCV 68.8±11.3 fl, MCH 21.8±4.6 pg, RDW 18.5±4.0%, 혈청 철 25.0±15.1 ㎍/㎗, 혈청 총철결합능력 422.0±62.7 ㎍/㎗, ferritin 6.7±2.3 ㎍/㎗이었다. 3) 환자들이 호소하는 증상들은 월경과다가 36.9%, 현기증이 34.6%, 만성두통 32.3%, 만성피로 17.7% 그리고 소화기계 장애가 16.9%를 나타내었다. 4) 빈혈의 주요 원인으로서는 월경과다가 75례(57.7%), 위십이지장 궤양이 18례(13.8%), 사춘기/임신으로 인한 철의 요구량 증가가 19례(14.6%)였으며 치질로 인한 출혈이 11례(8.5%)이었고 원인불명이 17례였다. 5) 대상환자들을 혈색소치 9.0 g/㎗를 기준으로 하여 두 그룹으로 분류하였으며 혈색소가 9 g/㎗이상인 경한 빈혈 그룹에서 혈색소와 MCV는 유의한 상관관계(r=0.6584, p<0.05)가 있었으며, 혈색소가 9 g/㎗ 미만인 중한 빈혈 그룹에서 혈색소와 MCHC가 유의한 상관관계(r=0.4305, p<0.05)를 나타내여 경한 빈혈에서는 MCV가 중한 빈혈에서는 MCHC가 의미가 있음을 시사하고 있다. 5) RDW와 MCV는 두 그룹 모두에서 반비례 관계를 보였지만, 빈혈이 경한 그룹에서는 통계적으로 유의한 상관관계(r=-0.2985, p<0.05)를 보였고, 빈혈이 중한 환자그룹에서는 통계적으로 상관관계(r=-0.1208, p<0.05)가 유의하지 않아서, IDA 초기에는 MCV와 RDW가 의미가 있겠지만 중한 빈혈인 경우에는 의미가 없음을 보여 주었다. 6) 새로운 지표 MCV/RDW 비값이 fe/TIBC 포화도와 상당히 유의한 양의 상관관계(r=0.4763, p<0.005)가 있었고 5.1 이하인 경우에는 85.4%의 민감도로 IDA를 진단할 수 있겠다. 이상의 결과에서 혈청 철의 생화학적 지표에 앞서 단순한 일반 혈액검사에서 MCV/RDW를 계산함으로써 IDA의 선별검사로 사용할 수 있으며 5.1 이하의 범위에서 IDA를 진단을 한다면 85.4%의 민감도로 예측할 수 있다. To evaluate the laboratory findings in patients with iron deficiency anemia(IDA) at initial presentation and to define useful screening tests such as RBC indices and markers of serum iron, we studied 130 patients with the diagnosis of IDA who visited Ajou University Hospital from July 1994 to January 1996. The results of the study are as follows; 1) The mean age of IDA patients were 36.3 ±11.6 years old and ranged from 12 to 65 years old. 2) The mean laboratory values at initial visits were hemoglobin 8.3±2.0 g/dl, hemalocrit 26.0±5.4%, mean corpuscular volume 68.8±11.3 fl, mean corpuscular hemoglobin 21.8±4.6 pg, RDW 18.5±4.0%, serum iron 25.0±15.1 ㎍/dl, TIBC 422.0±62.7 ㎍/dl and serum feiritin 6.7±2.3 ㎍/dl. 3) Frequent symptoms and signs were menorrhagia(36.9%), dizziness(34.6%), headache(32.3%), easy fatigability(17.7%) and G-Ⅰ trouble(16.9%). 4) Frequent causes of IDA were menorrhagia in 75(57.7%) cases, gastrointestinal ulcer in 18(13.8%) cases, increased demand for iron due to pregnancy and growth spun in 19(14.6%) cases, hemorrhoidal bleeding in 11(8.5%) cases. 5) When patients were divided into two groups(Hb≥9 g/dl as mild, Hb<9 g/dl as severe anemia), the group with Hba.9 g/dl showed a significant correlation between hemoglobin and MCV(r=0.6584, p<0.05). However, in the group with Hb<9 g/dl, there was a significant correlation between hemoglobin and MCHC(r=0.4305, p<0.05). 5) Negative correlation was observed between MCV and RDW in both groups, <although statistically significant in the mild group(r=-0.2985, p<0.05) but not significant in the severe group(r=-0.1208, p>0.05)> indicating more microcytic in RBC index and anisocytosis in RBC shape. 6) MCV/RDW ratio was correlated significantly with Fe/TIBC saiuration(r=0.4763, p<0.005) and MCV/RDW ratio of less than 5.1. This is lo be the most sensitive index in the diagnosis of IDA, sensitivity 85.4% in both mild and severe groups. In conclusion, in addition to serum iron markers, MCV, MCHC and RDW have been used as screening tests and prediction for IDA. We suggest that the ratio of MCV/RDW(fl/%) less than 5.1 is one of the most sensitive index in screening and diagnosing IDA with 85.4% sensitivity rate.

HCV, Alcoholic : PO-14 ; The significance of megaloblastic change during treatment of hepatitis C patients

( Haak Cheoul Kim ),( Eun Young Cho ),( Young Bum Cho ),( Ji Won Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.1

Background and Aims: For Hepatitis C virus (HCV) infection, pegylated interferon and ribavirin treatment are used as standard treatments, but they can make various hematologic adverse events. If such an adverse event arises, does should be reduced dosage, which may drop the treatment response. Also it is often reported that anemia occurrence can be an indicator of the good treatment response. We confirmed that Mean corpuscular volume (MCV) increase was not unusual during the HCV standard treatment and tried to find out the meaning of MCV increase during the process of HCV treatment. Methods: The subjects of the study were 60 outpatients who visited Wonkwang University Hospital because of chronic C viral hepatitis and were treated with pegylated interferon and ribavirinfrom from 2005 to 2010 and whose SVR could be identified. The medical records of the subjects were analyzed. CBC, blood chemistry, HCV RNA (copies/mL), T4 free and TSH were reviewed at 0, 4, 12, 24, 48 and 72 week after the treatment, and aspartate aminotransferase to platelet ratio index(APRI) was calculated based on the blood test result. Additionally, abdomen ultrasound scan was performed to check if there is a cirrhotic change. Results: 1. The subjects were 31 males and 29 females by gender, and 19 Genotype 1, 40 Genotype 2 and 1 Genotype 3 by Genotype. 24 patients were treated with interferon α2a, and 36 with pegylated interferon α2b. Regarding ribavirin dose, 11 patients had less than 800mg, 37 patients had 800mg ~ <1000mg, and 12 patients had ≥1000mg. Ten patients showed significant drinking history (80 g/day). 2. When classifying the group with the baseline of MCV 95fL before the treatment (Group 1, MCV< 95 fL; Group 2, MCV ≥95 fL) 16 subjects showed over 95 fL MCV, and there was no significantly different factors between groups (all≥0.05). The only significantly different factor was average age such as 59.1±13.5 in Group 1 and 64.0±7.19 in Group 2, which showed borderline significance (p=0.076). 3. Regarding the increase of MCV during the treatment, it increased regardless of Genotype at 4, 12, and 24 weeks and decreased from 48 weeks. (Genotype 1: 90.92±3.62, 90.95±4.03, 94.52±6.74, 97.43±7.31, 93.68±5.36, 91.36±5.56 in week 0, week 4, week 12 (p=0.000), week 24 (p=0.024), week 48, week 72, retrospectively; Genotype 2: 92.26±5.87, 93.54±5.66, 97.13±6.55, 98.08±7.11, 92.72±4.82, 92.58±4.99 in week 0, week 4 (p=0.000), week 12 (p=0.000), week 24 (p=0.000), week 48, week 72, retrospectively) 4. There was statistically significant difference in Hemoglobin (week 0, 2, 4, 12, 24) and platelet count (week 0, 2, 12, 24, 48, 72) and albumin (week 0, 4, 12, 24, 48, 72) (all p≤0.05) when dividing group with the standard of MCV ≥100fL (Group 3, MCV< 100 fL; Group 4, MCV ≥100 fL), which showed significant correlation when it was divided by APRI >0.08. Group 4 had more patients who showed cirrhotic changes on the sonography comparing to Group 3 (3/29(21.4%) in Group 3, 11/31(78.6%) in Group 4, p=0.021). Conclusions: Although MCV increase during the treatment was not related with the treatment response, it seemed to be related with chronicity of hepatitis. Thus, such patients should be closely observed even after the termination of the treatment.

HCV, Alcoholic : PO-14 ; The significance of megaloblastic change during treatment of hepatitis C patients

( Haak Cheoul Kim ),( Eun Young Cho ),( Young Bum Cho ),( Ji Won Kim ) 대한간학회 2012 춘·추계 학술대회 (KASL) Vol.2012 No.-

Background and Aims: For Hepatitis C virus (HCV) infection, pegylated interferon and ribavirin treatment are used as standard treatments, but they can make various hematologic adverse events. If such an adverse event arises, does should be reduced dosage, which may drop the treatment response. Also it is often reported that anemia occurrence can be an indicator of the good treatment response. We confirmed that Mean corpuscular volume (MCV) increase was not unusual during the HCV standard treatment and tried to find out the meaning of MCV increase during the process of HCV treatment. Methods: The subjects of the study were 60 outpatients who visited Wonkwang University Hospital because of chronic C viral hepatitis and were treated with pegylated interferon and ribavirinfrom from 2005 to 2010 and whose SVR could be identified. The medical records of the subjects were analyzed. CBC, blood chemistry, HCV RNA (copies/mL), T4 free and TSH were reviewed at 0, 4, 12, 24, 48 and 72 week after the treatment, and aspartate aminotransferase to platelet ratio index(APRI) was calculated based on the blood test result. Additionally, abdomen ultrasound scan was performed to check if there is a cirrhotic change. Results: 1. The subjects were 31 males and 29 females by gender, and 19 Genotype 1, 40 Genotype 2 and 1 Genotype 3 by Genotype. 24 patients were treated with interferon α2a, and 36 with pegylated interferon α2b. Regarding ribavirin dose, 11 patients had less than 800mg, 37 patients had 800mg ∼ <1000mg, and 12 patients had ≥1000mg. Ten patients showed significant drinking history (80 g/day). 2. When classifying the group with the baseline of MCV 95fL before the treatment (Group 1, MCV< 95 fL; Group 2, MCV ≥95 fL) 16 subjects showed over 95 fL MCV, and there was no significantly different factors between groups (all≥0.05). The only significantly different factor was average age such as 59.1±13.5 in Group 1 and 64.0±7.19 in Group 2, which showed borderline significance (p=0.076). 3. Regarding the increase of MCV during the treatment, it increased regardless of Genotype at 4, 12, and 24 weeks and decreased from 48 weeks. (Genotype 1: 90.92±3.62, 90.95±4.03, 94.52±6.74, 97.43±7.31, 93.68±5.36, 91.36±5.56 in week 0, week 4, week 12 (p=0.000), week 24 (p=0.024), week 48, week 72, retrospectively; Genotype 2: 92.26±5.87, 93.54±5.66, 97.13±6.55, 98.08±7.11, 92.72±4.82, 92.58±4.99 in week 0, week 4 (p=0.000), week 12 (p=0.000), week 24 (p=0.000), week 48, week 72, retrospectively) 4. There was statistically significant difference in Hemoglobin (week 0, 2, 4, 12, 24) and platelet count (week 0, 2, 12, 24, 48, 72) and albumin (week 0, 4, 12, 24, 48, 72) (all p≤0.05) when dividing group with the standard of MCV ≥100fL (Group 3, MCV< 100 fL; Group 4, MCV ≥100 fL), which showed significant correlation when it was divided by APRI >0.08. Group 4 had more patients who showed cirrhotic changes on the sonography comparing to Group 3 (3/29(21.4%) in Group 3, 11/31(78.6%) in Group 4, p=0.021). Conclusions: Although MCV increase during the treatment was not related with the treatment response, it seemed to be related with chronicity of hepatitis. Thus, such patients should be closely observed even after the termination of the treatment.

연령 증가에 따른 평균 적혈구 용적의 증가

제수정,서철원,지현숙 大韓血液學會 1992 大韓血液學會誌 Vol.27 No.2

EPPRV를 사용한 전자 MCV의 작동 지연 시간 분석

정황훈(Hwang Hun Jeong),이민수(Min Su Lee) 한국자동차공학회 2021 한국자동차공학회 부문종합 학술대회 Vol.2021 No.6

건설기계의 자동화는 인구의 노령화 및 숙련공 감소에 따른 생산량 감소 대응 및 위험한 장소에서의 작업자 안전 확보를 목적으로 활용되고 있다. 건설 현장에서 가장 많이 사용되고 있는 굴착기 또한 전자 MCV를 활용하여 자동화가 이루어지고 있다. 기존 유압 굴착기는 엔진에 의해 구동되는 유압펌프와 MCV, 액추에이터(붐, 암, 버킷 실린더, 스윙 모터, 주행 모터 및 어테치먼트용 유압 서비스 라인)로 구성되어지며, 이 액추에이터의 속도와 방향은 조이스틱의 동작에 따라 조절되는 파일럿 압력에 의해 동작하는 MCV에 의해 제어되어진다. 전자 MCV는 MCV에 공급되는 파일럿 압력을 EPPRV를 활용하여 공급하는 밸브 시스템으로 전기적인 신호를 통해 유압 굴착기를 자동화할 수 있도록 한다. 전자 MCV를 유압 굴착기에 활용할 경우 기존 시스템에 비하여 액추에이터의 응답 지연이 늘어나게 된다. 기존 유압 굴착기에서는 파일럿 압력을 on-off 밸브로 제어함으로써 밸브가 열릴 때 MCV의 스풀에 공급되는 유량이 EPPRV에 비해 크기 때문에 액추에이터는 EPPRV를 사용한 시스템보다 빠른 반응을 보인다. 하지만 EPPRV를 사용한 시스템은 기존 시스템의 지연시간에 EPPRV가 설정된 압력에 이르기까지의 지연 시간이 추가되기 때문에 운전자가 느낄 수 있을 만큼 액추에이터의 동작 지연이 발생한다. 본 발표는 굴착기 자동화를 위한 기초 연구로, 전자 MCV가 설치된 굴착기의 동작으로부터 시스템의 응답 지연을 분석하는 것을 목적으로 한다.

알콜중독환자에서의 평균적혈구용적에 관한 고찰

권석운,박성호,나덕렬,최상운,조한익 대한임상병리학회 1992 대한임상병리학회지 Vol.12 No.3

MCV와 RDW를 이용한 복합유기용제 취급 근로자의 빈혈 유형에 관한 조사

김성준,엄상화,김대환,이채언,전진호,김성천,배기택,박형종,Kim, S.J.,Ohm, S.H.,Kim, D.H.,Lee, C.U.,Chun, J.H.,Kim, S.C.,Pae, K.T.,Park, H.J. 대한예방의학회 1992 예방의학회지 Vol.25 No.2

The hematologic effect by low-concentration and repeated exposure to mixed organic solvent remains obscure. This study was performed to evaluate the hematologic effect by mixed organic solvent exposure, especially on the type of anemia by mean corpuscular volume(MCV) and red blood cell distribution width(RDW). The subject were 64 organic solvent workers(male 4, female 60) and 78 general workers(male 18, female 60) who showed anemic tendency in worker's health examination which carried out by Pusan Paik Hospital from January to December, 1988. The author gained some hematologic findings(hemoglobin, hematocrit, RBC, WBC, MCV, MCHC, platelet count, ESR, RDW) from auto-analysis method by coulter counter S plus IV, and compared the type of anemia by MCV and RDW. In the pilot study for estimating the prevalence of anemia according to the type of b, the prevalence rate was higher in organic solvent workers than in public officials as 10.9% and 4.1% respectively. There were statistical significance in the value of hemoglobin, hematocrit, MCV, MCHC, platelet count, ESR, RDW between the two study groups with more severe anemic tendency in organic solvent workers. The type of anemia in organic solvent workers was microcytic and anisocytic with the mean value of $68.28{\pm}8.3fL$ of MCV and $19.1{\pm}4.0%$ of RDW.