편백나무 건조 중 발생하는 휘발성 유기화합물의 항진균 및 항산화 활성

서영준,이재원,Seo, Young-Jun,Lee, Jae-Won 한국미생물학회 2012 미생물학회지 Vol.48 No.4

In this study, we evaluated antifungal and antioxidant activities of condensed liquid of volatile organic compounds generated during the drying process of Chamaecyparis obtuse. Condensed liquid of volatile organic compounds were extracted with hexane and ethyl acetate, respectively. The extracts were used for antifungal activity at range from 100 to 500 mg/L Antifungal activity of extracts increased as the extracts concentration increased, the activity against tested dermatophytes was high at 500 mg/L. Among extract solvents, antifungal activity was higher at hexane extract than the ethyl acetate. Especially, the antifungal activity against Trichophyton rubrum was higher than other tested dermatophytes. Antioxidant activity was 90% above 25 mg/L regardless of the extractive solvents, while the hexane extract showed high antioxidant activity below 25 mg/L. Major compounds of hexane extract were torreyol, alpha-cadinol, and tau-cadinol. However, major compounds of ethyl acetate extract were alpha-amorphene, alpha-cadinol, and gamma-cadinene. 본 연구는 편백나무 건조 중 발생하는 휘발성 유기화합물로 구성된 응축수에 대해 항진균 및 항산화 활성을 평가하였다. 응축수는 hexane과 ethyl acetate로 각각 추출하였으며 추출액의 농도 100-500 mg/L에서 피부사상균에 대한 항진균 활성을 측정하였다. 추출액 농도가 증가할수록 항진균 효과도 증가하였으며 500 mg/L에서는 모든 피부사상균에 대해 높은 항진균 활성을 나타냈다. 추출용매에 따라서는 hexane으로 추출하였을 경우 높은 활성을 나타냈으며, 특히 Trichophyton rubrum에 대한 항진균 활성이 높았다. 항산화 활성을 측정한 결과 25 mg/L 이상의 농도에서는 90% 이상의 항산화 활성을 나타냈으며 추출용매에 따른 차이는 거의 없었다. 하지만 25 mg/L 이하의 농도에서는 hexane 추출물에서 높은 항산화 활성을 나타냈다. 각각의 추출물 구성성분을 분석한 결과 hexane 추출물의 주요 구성성분은 torreyol, alpha-cadinol, tau-cadinol이며, ethyl acetate 추출물의 주요 구성성분은 alpha-amorphene, alpha-cadinol, gamma-cadinene으로 나타났다.

Inhaled essential oil from <i>Chamaecyparis obtuse</i> ameliorates the impairments of cognitive function induced by injection of β-amyloid in rats

Bae, Donghyuck,Seol, Heejin,Yoon, Ho-Geun,Na, Ju-Ryun,Oh, Kyonyeo,Choi, Chul Yung,Lee, Dong-wook,Jun, Woojin,Youl Lee, Kwang,Lee, Jeongmin,Hwang, Kwontack,Lee, Yoo-Hyun,Kim, Sunoh Informa Healthcare 2012 PHARMACEUTICAL BIOLOGY Vol.50 No.7

<P><I>Context: Chamaecyparis obtusa</I> Sieb. & Zucc., Endlicher (Cupressaceae) forest bathing or aromatherapy has been shown in various studies to have biological functions such as anticancer, antiallergies, antiinflammatory, and antioxidant activity. However, no reports exist on the pharmacological or biological activities of the essential oil of <I>C. obtusa</I> (EOCO) or its effects on central nervous system.</P><P><I>Objective</I>: The aggregation and formation of β-amyloid peptides (Aβ) into fibrils are central events in the pathogenesis of Alzheimer’s disease (AD), and overproduction and aggregation of Aβ into oligomers have been known to trigger neurotoxicity. In this study, we investigated the effects of inhaled EOCO on cognitive function and neuronal apoptosis in rats intrahippocampally injected with Aβ.</P><P><I>Materials and methods</I>: To model AD, 4 μg of aggregated Aβ was injected into the hippocampus. To test the effects of EOCO, behavioral performance in the Morris water maze was tested 4 days after injection. After behavioral testing, brain sections were prepared for TTC staining and TUNEL assay.</P><P><I>Results</I>: Inhaled EOCO protected spatial learning and memory from the impairments induced by Aβ<SUB>1-40</SUB> injection. In addition, the behavioral deficits accompanying Aβ<SUB>1-40</SUB>-induced AD were attenuated by inhalation of EOCO. Furthermore, acetylcholinesterase (AChE) activity and neuronal apoptosis were significantly inhibited in rats treated with Aβ<SUB>1-40</SUB> and EOCO compared to rats treated only with Aβ<SUB>1-40</SUB>.</P><P><I>Discussion and conclusion</I>: EOCO suppressed both AD-related neuronal cell apoptosis and AD-related dysfunction of the memory system. Thus, the results of this study support EOCO as a candidate drug for the treatment of AD.</P>

Antibacterial activity of Chamaecyparis obtuse Extract and Profile of Antimicrobial Agents Resistance for Methicillin-Resistant Staphylococcus aureus

염종화 대한의생명과학회 2024 Biomedical Science Letters Vol.30 No.1

In vitro antimicrobial activities of hot water extracts of Chamaecyparis obtuse, for methicillin-resistant Staphylococcus aureus (MRSA) was compared to commonly used conventional antimicrobial agents. All MRSA was susceptible to linezolid or vancomycin, but also to erythromycin. MIC range and MIC90 to erythromycin, clindamycin, levofloxacin, tetracycline for MRSA were each 4 μg/mL, 2 ~ >128 μg/mL, ≤0.06 ~ >128 μg/mL, 0.25 ~ >128 μg/mL, 0.25~64 μg/ mL and 4 μg/mL, .128 μg/mL, >128 μg/mL, >128 μg/mL, 64 μg/mL. The hot water extracts of leaf of C. obtuse had the lowest MIC range, MIC50, and MIC90 (0.125 μL/mL) for the MRSA tested, and it was possible more potent than various conventional antimicrobial agents. Screen antibacterial drug candidate with high antibacterial activity such as derivatives of C. obtuse leaf extract such as terpinen-4-ol or using combined therapy with commercialized antibacterial agents will likely be helpful in treating refractory MRSA infections.

The effects of essential oil of Chamaecyparis Obtuse impregnant fabric for atopic dermatitis

( Kwang Ho Yoo ),( Chang Kwun Hong ),( Tae Rin Kwon ),( Myeung Nam Kim ),( Hee Su Kim ),( Dong Sik Bang ),( Beom Joon Kim ) 대한피부과학회 2015 대한피부과학회 학술발표대회집 Vol.67 No.2

Background: The essential oil of Chamaecyparis obtuse (EOCO) has been used as an alternative treatment modality for allergy-related diseases due to their immune modulating activity. Textiles have the longest and most intense con〓tact with the human skin, and functional textiles with intrinsic properties such as anti-oxidative and anti-microbial activities have been gaining importance in medical applications. Specially designed textiles may support AD treatment and may improve the quality of life of AD patients.Objectives: Here, the effect of EOCO delivered through fabric on AD was investigated using NC/Nga mice as animal models. Methods: The fabric was applied 6 hours a day for 14 days in the experimental group. Results: The EOCO group had a significantly reduced modified SCORAD score, TEWL and serum IgE. Amongthe inflammatory cytokines analyzed, only the mean values of RANTES (regulated on activation, normal T cell expressed and secreted) were detected as low compared with the other control groups. And histological analysis of the skin also revealed that EOCO group reducedepidermal hyperplasia (thickness measurement, p<0.05) with recovery of the protein expression of filaggrin, involucrin and loricrin. Conclusion: It was confirmed that EOCO fabric has anti-atopic and anti-inflammatory activity. Based on the study data, it is suggested that EOCO fabric may be useful as an alternative functional textile for people suffering from AD.

편백나무 추출액이 돈사 공기 중의 냄새물질 농도 및 냄새기여도에 미치는 효과

황옥화,조성백,이민희,김기현,한덕우,이상룡,곽정훈 한국냄새환경학회 2016 실내환경 및 냄새 학회지 Vol.15 No.4

The objective of this experiment was to evaluate the effect of Chamaecyparis obtus extract to reduce odoremissions released from the swine feeding operations. Finisher pigs [Landrace × (Yorkshire × Duroc)] with aninitial body weight averaging 50 kg were housed separately in two rooms with eighty eight pigs in each room ata swine feeding operation site. C. obtus extract was sprayed in the room by ceiling sprayer for one minute attwice per day during two months. Concentrations of odorous compounds from air in the room of the swine feedingoperation were analyzed at four times during two months. Levels of butyric acid, valeric acid, i-butyric acid, ivalericacid, skatole, methylmercaptan, and trimethylamine tended to decrease in C. obtus extract spray treatmentgroup compared to the non-spray treatment group (P > 0.05). Odor activity values of butyric acid, valeric acid,skatole and trimethylamine were higher than other odorous compounds and decreased by 72%, 76%, 54% and20%, respectively, in C. obtus extract spray treatment group compared to the non-spray group. Taken together,C. obtus extract showed an odor reducing capability in the air of a swine feeding operation suggesting that itpossesses anti-bacterial properties as well as having a dust removal and masking effect.

추출방법에 따른 편백 정유의 향기 성분 비교 및 아토피 개선에의 응용

임금숙(Geum-Sook Lim),김란(Ran Kim),조훈(Hoon Cho),문영숙(Young-Sook Moon),최창남(Chang-Nam Choi) 한국생물공학회 2013 KSBB Journal Vol.28 No.2

Volatile flavor compounds of Chamaecyparis obtuse essential oil were extracted by simultaneous steam distillation extraction (SDE) and supercritical fluid extraction (SFE) and analyzed by GC-MS. A total of 48 and 50 components were identified in essential oil by SDE and SFE, respectively. Monoterpenes, oxygenated monoterpenes, sesquiterpenes, oxygenated sesquiterpenes, and diterpenes in essential oil by SDE were 37.24, 10.9, 9.61, 0.22, and 0.22%, respectively. In the case of SFE, they were 19.1, 23.3, 22.66, 1.31, and 10.57%, respectively. Antioxidant activities were increased with the increase of essential oil up to 80 μL /mL, irrespective of extraction method. Especially, when the essential oil concentration extracted by SDE was increased from 20 to 80 μL /mL, the antioxidant activity was increased from 10.5 to 55.1%. However, over 80 μL/mL of essential oil, an equilibrium state was maintained. In the case of essential oil extracted by SFE, it was decreased compared to that of SDE. For the improvement of atopic dermatitis, various cosmetics such as an ato-cide soap, ato-cide spray, and ato-cide lotion containing essential oil extracted by SFE were tested. About over 90% was useful for the improvement of atopic dermatitis after 4 weeks of clinical trial targeting 40 female adults. These results demonstrate that ato-cide soap, ato-cide spray, and atocide lotion containing essential oil extracted by SFE could be used in functional cosmetics.

입지환경인자를 바탕으로 한 편백의 지위지수 추정에 관한 연구

김대현 ( Dae Hyun Kim ),김의경 ( Eui Gyeong Kim ),이성기 ( Sung Gi Lee ),정영교 ( Young Gyo Chung ),정진현 ( Hyun Jeong Jin ) 한국환경과학회 2008 한국환경과학회지 Vol.17 No.8

편백의 다신초 유도 및 발근을 통한 식물체 재분화

김용욱,김지아,이나념 한국식물생명공학회 2019 JOURNAL OF PLANT BIOTECHNOLOGY Vol.46 No.4

A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3- butyric acid.

편백의 다신초 유도 및 발근을 통한 식물체 재분화

김지아,이나념,김용욱,Kim, Ji Ah,Lee, Na-Nyum,Kim, Yong Wook 한국식물생명공학회 2019 식물생명공학회지 Vol.46 No.4

본 연구에서는 편백의 다신초 유도를 통한 기내 식물체 재분화기술을 개발하고자 본 연구를 수행하였다. 이를 위해 먼저 다신초 유도를 위한 적정 싸이토키닌의 종류 및 농도별 처리에서 1/2LM배지에 0.2 mg/L zeatin의 처리구에서 가장 높은 73%였으며, 절편체 당 평균 3.9개 다신초가 유도되었다. 그리고 싸이토키닌의 고농도→저농도의 순차적 처리의 경우 5.0 mg/L→0.5 mg/L zeatin 처리구에서 100%의 신초 유도율과 절편체 당 17.1개의 가장 많은 다신초가 유도되어 다신초 유도를 위한 최적 조건으로 확인되었다. 신초의 길이신장을 위한 배지는 LM배지에서 배양할 경우 2.62 cm로 가장 좋은 길이생장의 결과를 얻었다. 신초정단부의 갈변화 현상을 억제하기 위해 항산화제 처리 결과 0.5% MES처리구에서 37.5%의 갈변화증상만이 보여 무처구에 비해 2.5배 이상 갈변화 억제 효과가 있었으며, 그에 따른 줄기 길이 신장 또한 1.3 cm로 가장 좋게 나타났다. 신초의 발근은 0.2 mg/L IBA처리구에서 65% 발근율과 3.9개 뿌리가 유도되어 가장 효과적인 처리구였으며, 이렇게 재분화된 기내식물체는 순화를 거쳐 현재 생육포지에서 활발히 생육 중에 있다. A protocol for the in vitro propagation of Chamaecyparis obtusa was established in the present study. Multi-shoots were initiated from apical shoot explants from germinants after 10 weeks of culture on Litvay medium (LM) supplemented with different concentrations of cytokinin. The effects of pre-treatment with high concentrations of cytokinin and varying concentrations (0.2 to 5.0 mg/L) of zeatin on in vitro shoot elongation and shoot multiplication were investigated. Optimal shoot growth was achieved on LM medium, with over 10-mm shoots after 10 weeks of culture. In the anti-browning tests, ethanesulfonic acid triggered the least browning in the shoot tips. The highest multi-shoot induction was observed in the 0.5-mg/L zeatin treatments, which yielded 80% induction of shoots after 10 weeks of culture, and maximum shoot elongation was observed in the LM basal medium without the hormone. The highest rooting rates were 65% under 0.2 mg/L indole-3-butyric acid.

편백나무 추출액의 항균 및 항산화 활성

김보경(Bo Kyung Kim),강정현(Jeong Hyeon Kang),오근혜(Geun Hye Oh),황지영(Ji-Young Hwang),장석위(Seok Oui Jang),김미향(Mihyang Kim) 한국생명과학회 2019 생명과학회지 Vol.29 No.7

본 연구에서는 편백나무(Chamaecyparis obtusa) 추출액의 항산화 활성 및 항균활성을 검토하였다. 편백나무 추출액의 항산화 활성을 평가하기 위하여 DPPH radical 소거능, ABTS radical 소거능 및 SOD 유사 활성을 분석하였다. 그 결과, 편백나무 추출액의 DPPH radical 및 ABTS radical 소거 활성은 농도 의존적으로 증가하였으며, 50μl/ml 농도에서 78% 및 62%의 최대 활성을 나타내었다. 또한, 편백나무 추출액은 높은 SOD 유사 활성을 보였으며, 50 μl/ml 농도에서 92.85 %의 최대 활성을 나타내었다. 한편, 편백나무 추출액의 항균 활성을 측정하기 위해 Paper disc agar diffusion 법을 이용하여 식중독 및 질병을 일으키는 6종의 균주에 대하여 검토하였다. 편백나무 추출액은 B. cereus, E. coli, L. monocytogenes, S. aureus, S. typhi, V. parahaemolyticus에 대한 항균 활성이 나타났고, 이중 B. cereus에 대하여 가장 강한 항 박테리아 활성을 보였다. 이상과 같이 항균 활성이 나타난 6종의 균주에서 편백나무 추출액의 MIC 및 MBC는 30~40 μl/ml로 확인되었다. 이러한 결과는 편백나무 추출액을 이용하여 식중독과 같은 병원균의 성장을 억제하는 항균 소재로 개발한다면 산업적 가치가 높을 것으로 생각된다. In this study, we investigated the biological antioxidant and antibacterial activity of Chamaecyparis obtusa (C. obtuse) extracts by measuring DPPH radical scavenging and ABTS radical scavenging, and SOD-like activities. The DPPH and ABTS radical scavenging activities were increased in a dose-dependent manner, with maximum activities of 78% and 62% at an extract concentration of 50 μl/ml. The C. obtusa extracts also showed high SOD-like activity, with a maximum activity of 92.85% at a concentration of 50 μl/ml. The antibacterial activities of C. obtusa extracts were measured against six types of bacteria known to cause food poisoning and disease. Antibacterial activity was investigated against three gram-positive and three gram-negative bacteria using the paper disc agar diffusion method. The C. obtusa extracts showed antibacterial activities against B. cereus, E. coli, L. monocytogenes, S. aureus, S. typhi and V. parahaemolyticus, among which the activity against B. cereus was greatest. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of C. obtusa extracts were 30–40 μl/ml for the 6 strains that showed an antimicrobial response by the paper disc agar diffusion method. These results suggest that C. obtusa extracts could serve as potential antibacterial agents to inhibit the growth of pathogens responsible for food poisoning and disease.