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벼꽃으로부터 두 개의 calmodulin cDNA 유전자의 분리 및 특성 연구
고보경,고명수,김유정,정일선,최영주 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-
Ca^(2+)은 신호전달과정에서 중요한 작용을 하는데 CaM은 이러한 Ca^(2+)이은의 modulator로서 중요한 작용을 하는 것으로 알러져 있다. 벼꽃의 cDNA library로부터 rice genomic DNA(RCaM-2)를 probe로 사용하여 30여개의 Positive clones을 얻어 이 중 두 개의 벼꽃 calmodulin CDNA clone을 분리하였다. RFCaMs CDNA clone의 총염기수는 RFCaM-3은 831p, RFCaM-4는 751bp이고 open reading frame은 각각 149개의 아미노산으로 구성되어 있다. RFCaM-3 과 RFCaM-4의아미노산은 동일하지만 nucleotide 순서는 상당한 차이를 나타내고 있다. RFCaMs CDNA clone의 open reading flame은 protein serine/threonine kinase에 보존되어 있는 catalytic domain을 가지고 있다 RfCaM-3은 5'-untranslated region은 105bp, 3'-untranslated region은 270bp이고 RfCaM-4는 5'-untranslated region은 78bp, 3'-untranslatedregion은 283bp이다. RFCaMs는 Arabidopsis와 70%, potato와는 92%, carrot과는 98%의 유사성을 보였다. Genomic southern blot분석에 의하면 벼의 clamodulin 유전자들이 7-8개의 multigene family로 존재하는 것으로 나타났다. Two different calmodulin (CaM) cDNAs (RFCaM-3 and RFCaM-4) were isolated from a rice flower cDNA library by screening with a rice genomic probe (RCaM-2). Both cDNAs are 90% sequence homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by RFCaM-3 and RFCaM-4 show identical sequence homology. Comparison of the deduced amino acid sequence of the RFCaMs cDNA clone have identity to Arabidopsis (70%), potato (92%), carrot (98%), and barley and soybean (98%). Southern analysis revealed that both cDNAs are encoded by different genes. Four intense and a few weaker bands were observed in the restriction fragments. Most restriction fragments showed high intensity hybridization signals under stringent conditions, suggesting the presence of a small multigene family for CaM in the rice genome as in other plants.
동종 조혈모세포이식 후 발생한 크립토콕쿠스 척추염 1예
고윤호,임동준,이성수,조유경,이동건,최정현,김유진,민창기,김동욱,박정미,김춘추,신완식 대한감염학회 2001 감염 Vol.33 No.4
Skeletal cryptococcosis is an uncommon infection. Cryptococcus is a common cause of meningitis and infects 7∼10% of patients with AIDS. As well as AIDS, the infection may be seen in association with leukemia, lymphoma, Hodgkin's disease, sarcoidosis, tuberculosis and diabetes, also in patients on steroid medication. But there is no case report of skeletal cryptococcosis following allogeneic hematopoietic stem cell transplantation. A 40-year-old woman was admitted to the hospital because of low back pain. She had chronic myelogenous leukemia for 2 years and underwent allogeneic hematopoietic stem cell transplantation 8 months ago. She have been treated with steroid and cyclosporine orally because of chronic graft versus host disease. On examination she was afebrile and had posterior lower lumbar tenderness. But, she had no reduced strength of low extremities. Open biopsy was underwent. Histology demonstrated budding, round-to-oval, refractile yeast-like organisms within debris. The results of a lumbar puncture were unremarkable and cerebrospinal fluid culture failed to grow bacteria and yeast. The patient was treated with amphotericin B (1 gram) and AmBisome□ (2.8 gram) over 6 weeks. Three months after cessation of therapy, the patient was doing well.(Korean J Infect Dis 33:298∼301, 2001)
국내산 과일 및 채소 추출물을 이용한 체중 조절 관련 기능성 식품소재 탐색
김인혜;김종배;조강진;황유진;황경아;고정숙;엄애선 한양대학교 2011 韓國 生活 科學 硏究 Vol.31 No.1
Obesity can be defined as a disorder of lipid metabolism and the control of this process is regulated by enzymes involved in lipid metabolic pathway. Obesity is also characterized increases in the number and size of adipocyte differentiated from fibroblastic pre-adipocytes in adipose tissue. The aim of this study was to screen ethanol extracts of 21 fruits and vegetables cultivated in Republic of Korea for identifying natural sources of lipase inhibitor and to investigate the effects of several extracts resulting in suppression of triglyceride (TG) accumulation in 3T3-L1 cells. Among the tested extracts, Diospyros kaki L. showed the highest contents of total sugar and the content of total phenolic compounds was detected 17 species while there was not detected in Ixeris dentate root and Allium fistulosum C. etc. We were screened 26 extracts of fruits and vegetables for pancreatic lipase inhibitory activity compared with orlistat. The strongest lipase inhibitory activities were shown by extract of Allium sativum L. stem, Allium sativum L. bulb, Apteryx australis, Diospyros kaki L. Malus Pumila, Perilla frutescens and Prunus mume regardless of concentrations treated. Eight extracts including Brassica juncea var. integrilolia had no effect on lipase inhibition. During the differentiation of 3T3-L1 preadipocyte to mature adipocyte, the cells were treated with carrier or 7 extracts(1, 5 and 10 ㎍/ml), which was shown to highest lipase inhibitory effects. We were quantified lipid contents secreted mature adipocytes and stained with Oil Red O. All cells treated with the Apteryx australis and Prunus mume significantly decreased TG contents than the control. These findings suggested that the screened fruit and vegetable could be a promising source as the effective and safe lipase inhibitors for isolating bioactive ingredients and developing functional foods.
Yu, Tae-Kyung,Yun, Young-Joo,Lee, Ko On,Ahn, Kyung Jun,Suh, Jeong-Yong Elsevier 2012 FEBS letters Vol.586 No.10
<P><B>Highlights</B></P><P>► Phosphoryl groups in the biphosphorylated complex exhibit characteristic dynamics. ► The dynamics occurs in a millisecond time scale at the active site of HPr. ► The time scale is comparable to the phosphoryl transfer rate between EIN and HPr. ► The dynamics of HPr may be important to interact with multiple partner proteins.</P> <P><B>Abstract</B></P><P>The N-terminal domain of Enzyme I (EIN) and phosphocarrier HPr can form a biphosphorylated complex when they are both phosphorylated by excess cellular phosphoenolpyruvate. Here we show that the electrostatic repulsion between the phosphoryl groups in the biphosphorylated complex results in characteristic dynamics at the active site in a millisecond time scale. The dynamics is localized to phospho-His15 and the stabilizing backbone amide groups of HPr, and does not impact on the phospho-His189 of EIN. The dynamics occurs with the <I>k</I><SUB>ex</SUB> of ∼500s<SUP>−1</SUP> which compares to the phosphoryl transfer rate of ∼850s<SUP>−1</SUP> between EIN and HPr. The conformational dynamics in HPr may be important for its phosphotransfer reactions with multiple partner proteins.</P><P><B>Structured summary of protein interactions</B></P><P><B>EIN</B> and <B>HPr</B> bind by nuclear magnetic resonance (View Interaction).</P>
Yu, Kyung-Rok,Yang, Se-Ran,Jung, Ji-Won,Kim, Hyongbum,Ko, Kinarm,Han, Dong Wook,Park, Sang-Bum,Choi, Soon Won,Kang, Soo-Kyung,Schö,ler, Hans,Kang, Kyung-Sun Wiley (John WileySons) 2012 Stem Cells Vol.30 No.5
<P>CD49f (integrin subunit α6) regulates signaling pathways in a variety of cellular activities. However, the role of CD49f in regulating the differentiation and pluripotency of stem cells has not been fully investigated. Therefore, in this study, human mesenchymal stem cells (hMSCs) were induced to form spheres under nonadherent culture conditions, and we found that the CD49f-positive population was enriched in MSC spheres compared with MSCs in a monolayer. The expression of CD49f regulated the ability of hMSCs to form spheres and was associated with an activation of the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. Furthermore, the forced expression of CD49f modulated the proliferation and differentiation potentials of hMSCs through prolonged activation of PI3K/AKT and suppressed the level of p53. We showed that the pluripotency factors OCT4 and SOX2 were recruited to the putative promoter region of CD49f, indicating that OCT4 and SOX2 play positive roles in the expression of CD49f. Indeed, CD49f expression was upregulated in human embryonic stem cells (hESCs) compared with hMSCs. The elevated level of CD49f expression was significantly decreased upon embryoid body formation in hESCs. In hESCs, the knockdown of CD49f downregulated PI3K/AKT signaling and upregulated the level of p53, inducing differentiation into three germ layers. Taken together, our data suggest that the cell-surface protein CD49f has novel and dynamic roles in regulating the differentiation potential of hMSCs and maintaining pluripotency.</P>