Production of Bovine Nuclear Transfer Embryos Using Fibroblasts Transfected with Single-Chain Human Follicle-Stimulating Hormone Gene

Yoon, Ji Young,Kwon, Mo Sun,Kang, Jee Hyun,Ahn, Kwang Sung,Kim, So Seob,Kim, Nam-Hyung,Kim, Jin-Hoi,Kim, Teoan,Shim, Hosup Asian Australasian Association of Animal Productio 2009 Animal Bioscience Vol.22 No.2

Human follicle-stimulating hormone (hFSH) is a pituitary glycoprotein that regulates follicular development and ovulation. Clinically, hFSH has been used to induce follicular growth in infertile women. The hormone is composed of heterodimers, including a common ${\alpha}$ subunit among the gonadotropin family and a hormone-specific ${\beta}$ subunit. Since assembly of the heterodimer is a rate-limiting step in the production of functional hFSH, transgenic clone cows carrying a single-chain hFSH transgene may efficiently produce functional hormone. Genes encoding the ${\alpha}$ and ${\beta}$ subunits of hFSH were linked using the C-terminal peptide sequence from the ${\beta}$ subunit of human chorionic gonadotropin. Bovine fetal fibroblasts were transfected with the gene construct, including the goat ${\beta}$-casein promoter and a single-chain hFSH coding sequence. Transfected fibroblasts were transferred into enucleated oocytes, and individual nuclear transfer (NT) embryos developed to the blastocyst stage were analyzed for the transgene by polymerase chain reaction. Seventy eight blastocysts (30.8%) were developed from 259 reconstructed embryos. Among these blastocysts, the hFSH gene was detected in 70.8% (34/48) of the embryos. Subsequent transfer of hFSH-transgenic clone embryos to 31 recipients results in 11 (35.5%) early pregnancies. However, all fetuses were lost before reaching day 180 of gestation. The results from this study demonstrated that bovine NT embryos carrying single-chain hFSH could be produced, and further extensive studies in which NT embryos are transferred to more recipients may give rise to single chain hFSH-transgenic cows for biomedical applications.

Reduced Mitochondrial Properties in Putative Progenitor/Stem Cells of Human Keratinocytes

( Sung Eun Chang ),( Young Mi Kim Pak ),( Hae Woong Lee ),( Jee Ho Choi ),( Eun Jeong Jeong ),( Seung Ho Choi ),( Hyo Won Chang ),( Yoo Sam Chung ),( Sang Yoon Kim ) 대한피부과학회 2009 Annals of Dermatology Vol.21 No.4

Background: The characterization of progenitor/keratinocyte stem cells (KSC) remains an unachieved goal. A previous study showed that rapid adhering cells to collagen IV had the characteristics of putative progenitor/KSCs. Objective: The purpose of this study was to investigate the genetic expression of rapid adhering cells compared to non adhering cells to determine the characteristic of KSCs. Methods: We isolated rapid adhering cells representative of KSCs from non adhering cells representative of transient amplifying cells. In addition, we differentiated cells from human tonsilar keratinocytes utilizing the adhering capability of the KSCs to collagen IV. Annealing control primer based differentially displayed polymerase chain reaction (PCR) was performed as well as Western blot analysis. Results: The levels of mitochondria- related gene expression were low in the rapid adhering cells compared to the non adhering cells. Mitochondrial complex I, COX IV, peroxiredoxins (I, II and IV) and mitochondrial membrane potential were all low in the rapid adhering cells compared to the non adhering cells. Conclusion: Using an adhesion method on human collagen IV-coated plates, our results suggest that reduced mitochondrial function may be an important characteristic of KSCs. (Ann Dermatol 21(4) 364~368, 2009)

Efficacy and Safety of 1,064 nm Q-switched Nd:YAG Laser Treatment for Removing Melanocytic Nevi

( Yoon Jee Kim ),( Kyu Uang Whang ),( Won Bok Choi ),( Hyun Jo Kim ),( Jae Young Hwang ),( Jung Hoon Lee ),( Sung Wook Kim ) 대한피부과학회 2012 Annals of Dermatology Vol.24 No.2

Background: Until recently, the removal of melanocytic nevi has been performed with a CO2 laser or Er:YAG laser. These lasers have been useful for removing affected spots. However, enlargement of spots or some sequelae, including depressed or hypertrophic scars, could develop as unwanted results. The Q-switched Nd:YAG laser has been used to remove deep-seated melanocytes, such as Ota nevus or tattoos. However, there have been no previous experiments performed to test the efficacy and safety of this laser treatment for melanocytic nevi. Objective: The objective of this study was to investigate the efficacy and safety of the 1,064 nm Q-switched Nd:YAG laser for removing melanocytic nevi, including congenital nevomelanocytic and acquired nevomelanocytic nevi. Methods: Two thousand and sixty four Korean patients with small melanocytic nevi were treated with a Q-switched Nd:YAG laser from 2005 to 2009. High-resolution photographs were taken in identical lighting and positions before and after the six weeks of treatment to observe the procedural efficacy. Results: About 70% of the nevi treated using a 1,064 nm Q-switched Nd:YAG laser were completely removed after one session. The other 30% were completely treated within three sessions. The appearance of sequelae such as hollow scars noticeably decreased compared to the results seen in CO2 or Er:YAG laser treatments. Conclusion: Use of the 1,064 nm Q- switched Nd:YAG laser is a safe and effective treatment modality for melanocytic nevi. (Ann Dermatol 24(2) 162∼ 167, 2012)

The Association between Motor Laterality and Cognitive Impairment in Parkinson’s Disease

Jee-Eun Yoon,김지선,Jae-Young Seo,Jin Whan Cho,Jun-Sang Sunwoo,이경복,노학재,안무영 대한치매학회 2016 Dementia and Neurocognitive Disorders Vol.15 No.4

Background and Purpose The relationship between the side of motor symptoms and cognitive impairment has rarely been reported in Parkinson’s disease (PD). We aimed to estimate the influence of motor laterality on cognition in PD patients. Methods We enrolled 67 patients with PD, and they were divided into two groups according to side of symptom onset or predominant motor symptom presentation (right and left). Right-sided PD (RPD, 40) and left-sided PD (LPD, 27) patients underwent a neuropsychological battery exploring memory, attention/working memory, frontal/executive, visuospatial, and language functions. Student's t-test and Chisquare test have been carried out to compare the clinical and neuropsychological data between two groups. Results There were no significant differences in any neuropsychological test between the RPD and LPD groups, except for digit forward span test. RPD patients scored lower on the digit forward span test than LPD patients (5.43±9.49 vs. 6.15±1.38, p=0.045). Conclusions RPD patients seem to experience more difficulties in attention and working memory than did LPD patients. The laterality of motor symptoms is not a major determinant for cognitive impairment in PD patients but, we should consider differences of cognitive deficits depending on the side of motor symptoms to treat patients with PD.

[Thyroid & Endocrinology] Is Intraoperative Frozen Section Helpful in Diagnosis of FVPTCs?

Sung Yoon Jang,Anna Cho,Jung-Han Kim,Jun-Ho Choe,Jee Soo Kim 대한외과학회 2021 대한외과학회 학술대회 초록집 Vol.2021 No.11

Thematic Poster : TP-80 ; Incidence of Venous Thromboembolism in Head and Neck Surgery and Usefulness of Caprini Score in Korean Patients

( Sang Heon Kim ),( Sang Hoon Kim ),( Jang Won Sohn ),( Ho Joo Yoon ),( Dong Ho Shin ),( Sung Soo Park ),( Jae Hyung Lee ),( Byoung Hoon Lee ),( Youn Seup Kim ),( Jae Seuk Park ),( Young Koo Jee ) 대한결핵 및 호흡기학회 2014 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.118 No.-

Background: Postoperative venous thromboembolism (VTE) is a fatal condition in patients undergoing surgical procedure. The incidence of VTE and the usefulness of Caprini risk scoresfor predicting VTE in head and neck surgery have not been well established in Korean patients. Methods: In a prospective design, we enrolled subjects who underwent head and neck surgery from January to March 2013. Clinical characteristics and Caprini score were assessed at the day of operation. The development of VTE was monitored for the following 3 months after surgery. Results: Of 364 enrolled subjects, nine patients were identified to have VTE. The 3-month incidence rate of VTE in head and neck surgery was 2.5%. Three of these patients were in fatal condition requiring emergency embolectomy and one of them underwent extracorporeal membrane oxygenation before embolectomy. Caprini score in VTE patients was significantly higher than those without VTE (8.1 ± 5.1 vs. 3.9 ± 1.9, P = 0.048). Receiver-operating characteristic curve analysis revealed that the diagnostic accuracy of Caprini score was high with an area under the curve of 0.868 ±0.035. Conclusion: The incidence of VTE in patients with head and neck surgery was 2.47%. The Caprini risk score was useful in selecting high risk patients for VTE in head and neck surgery.

Effect of Cyclosporine on Peripheral Blood and Lesional Skin in Psoriatic Patients

( Sung Eun Chang ),( Woo Jin Yun ),( Ghil Suk Yoon ),( Jee Ho Choi ),( Bong Seong Kim ),( Soo Jong Hong ) 대한피부과학회 2007 Annals of Dermatology Vol.19 No.3

Background: Cyclosporine effectively suppresses immune responses and inhibits skin homing T cell responses in psoriasis. E-selectin is known to be up-regulated on vascular endothelium of inflammatory skin lesions such as psoriasis. Purpose: Based on our previous study that cyclosporine decreased lesional cutaneous lymphocyte antigen (CLA)+ T cells in psoriatic patients, we tried to find any change of CLA+ T cells in peripheral blood in psoriatic patients, since psoriasis is a disease of systemic T cell activation. Subjects and Methods: Peripheral blood of 8 patients with chronic plaque type psoriasis at 0, 3, 6, 12, 18 weeks after cyclosporine was examined by flow cytometry using anti-CLA antibody. Five skin biopsy samples at 0, 3, 6, 12, 18 weeks were immunohistochemically stained with anti E-selectin antibody. Results: Our results demonstrate that the number of CD3+ CLA+ and CD4+CLA+ T cells was significantly reduced in the peripheral blood at week 3, but gradually increased to the level of baseline at 18 weeks. In psoriatic skin lesions, with decrease of PASI score and CLA+ T cells number, the expression of E-selectin on the endothelial cells was gradually decreased throughout 18 weeks of therapy. Conclusion: These results suggest that cyclosporine suppresses the migration of skin homing T cells to psoriatic skin lesions, in part, through the inhibition of E-selectin on the endothelial cells. (Ann Dermatol (Seoul) 19(3) 106~111, 2007)

Synergistic toxicity with copper contributes to NAT2-associated isoniazid toxicity

Yoon Jihoon G.,Jang Dong Geon,Cho Sung-Gyu,Lee Chaeyoung,Noh Shin Hye,Seo Soo Kyung,Yu Jung Woo,Chung Hyeon Woo,Han KyeoRe,Kwon Soon Sung,Han Dai Hoon,Oh Jaeseong,Jang In-Jin,Kim Sang-Hoon,Jee Young-K 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

Anti-tuberculosis (AT) medications, including isoniazid (INH), can cause drug-induced liver injury (DILI), but the underlying mechanism remains unclear. In this study, we aimed to identify genetic factors that may increase the susceptibility of individuals to AT-DILI and to examine genetic interactions that may lead to isoniazid (INH)-induced hepatotoxicity. We performed a targeted sequencing analysis of 380 pharmacogenes in a discovery cohort of 112 patients (35 AT-DILI patients and 77 controls) receiving AT treatment for active tuberculosis. Pharmacogenome-wide association analysis was also conducted using 1048 population controls (Korea1K). NAT2 and ATP7B genotypes were analyzed in a replication cohort of 165 patients (37 AT-DILI patients and 128 controls) to validate the effects of both risk genotypes. NAT2 ultraslow acetylators (UAs) were found to have a greater risk of AT-DILI than other genotypes (odds ratio [OR] 5.6 [95% confidence interval; 2.5–13.2], P = 7.2 × 10−6). The presence of ATP7B gene 832R/R homozygosity (rs1061472) was found to co-occur with NAT2 UA in AT-DILI patients (P = 0.017) and to amplify the risk in NAT2 UA (OR 32.5 [4.5–1423], P = 7.5 × 10−6). In vitro experiments using human liver-derived cell lines (HepG2 and SNU387 cells) revealed toxic synergism between INH and Cu, which were strongly augmented in cells with defective NAT2 and ATP7B activity, leading to increased mitochondrial reactive oxygen species generation, mitochondrial dysfunction, DNA damage, and apoptosis. These findings link the co-occurrence of ATP7B and NAT2 genotypes to the risk of INH-induced hepatotoxicity, providing novel mechanistic insight into individual AT-DILI susceptibility.

Efficacy and safety of guselkumab compared with placebo and adalimumab in Korean patients with moderate to severe psoriasis: Post-hoc analysis from the phase Ⅲ, double-blind, placebo- and active comparator-controlled VOYAGE1/2 trials

( Sang Woong Youn ),( Tae Yoon Kim ),( Byung Soo Kim ),( Seung Chul Lee ),( Jeung Hoon Lee ),( Yong-beom Choe ),( Joo-heung Lee ),( Jee-ho Choi ),( Joo Young Roh ),( Seong Jin Jo ),( Eun-so Lee ),( Mi 대한피부과학회 2018 대한피부과학회 학술발표대회집 Vol.70 No.2

Background: The Phase Ⅲ studies, VOYAGE 1 and 2, were conducted to assess guselkumab for the treatment of patients with moderate to severe psoriasis. However, these studies included a worldwide study population. Objectives: To determine the efficacy and safety of guselkumab in Korean patients. Methods: This analysis included 28 patients from VOYAGE 1 and 98 patients from VOYAGE 2. In total, 126 patients were analyzed. Outcomes through week 28 were analyzed due to differences in design after week28. Results: Of 126 Korean patients randomized, 30 received placebo, 63 were assigned to guselkumab, and 33 received adalimumab. Guselkumab was superior to adalimumab in achieving an IGA 0 score (clear skin) at week 12 (31.7% vs 0.0%, p<0.001) and at week 24 (52.4% vs 21.2%, p=0.004), respectively. Among patients treated with guselkumab, 40 of 63 (63.5%) achieved a PASI75 response at week 8; at week 24, a significantly higher proportion of guselkumab patients achieved a PASI 75 compared to adalimumab patients (93.7% vs 66.7%, respectively, p<0.001). In addition, higher proportions of guselkumab patients achieved PASI 90 and PASI 100 than adalimumab patients at week 24 (PASI90; 73.0% vs 57.6%, p=0.168 / PASI100; 20.6% vs 15.2%, p=0.591, respectively). Through week 28, guselkumab and adalimumab showed comparable safety profiles. Conclusion: The efficacy and safety of guselkumab in Korean psoriasis patients through 28 weeks was consistent with findings reported for the VOYAGE1 and 2 trials.

Retroperitoneal fibrosis in the era of immunoglobulin G4-related disease

( Yoon Kyung Choi ),( Ji Hyun Yang ),( Shin Young Ahn ),( Gang Jee Ko ),( Se Won Oh ),( Myung Gyu Kim ),( Won Yong Cho ),( Sang Kyung Jo ) 대한신장학회 2019 Kidney Research and Clinical Practice Vol.38 No.1

Background: Retroperitoneal fibrosis (RPF) is a rare disease characterized by fibroinflammatory tissue in the periaortic or periiliac retroperitoneum, where it frequently encases ureters. There is emerging evidence that a subset of this disease is part of a spectrum of multisystemic autoimmune diseases collectively referred to as “immunoglobulin G4 (IgG4)-related disease”. Methods: We retrospectively analyzed 27 idiopathic RPF patients and identified a subset as IgG4-related RPF, which we categorized according to recently published comprehensive diagnostic criteria. We compared clinical and laboratory characteristics and response to treatment between the two groups. Results: Of 27 total patients, 16 (59.3%) were diagnosed as having IgG4-related RPF, and these were predominantly male. They were also significantly older and more likely to have other organ involvement, hydronephrosis, and postrenal acute kidney injury (AKI) compared to those with idiopathic RPF. However, there was no difference in response rate to systemic steroid treatment. Conclusion: IgG4-related RPF accounts for a substantial portion of RPF cases previously identified as “idiopathic RPF” in Korea. Clinical and laboratory characteristics of IgG4-related RPF are similar to those of idiopathic RPF except for a striking male predominance, older age, and higher incidence of postrenal AKI in IgG4-related RPF. More comprehensive, prospective studies are needed to clearly distinguish IgG4-related RPF from idiopathic RPF based on clinical manifestation and to further assess treatment response and long-term prognosis.