Tetrahydrobiopterin Inhibits PDGF-stimulated Migration and Proliferation in Rat Aortic Smooth Muscle Cells via the Nitric Oxide Synthase-independent Pathway

Xiaowen Jiang,김보경,Haiyue Lin,이창권,김정완,강현,이필영,원경종,정성호,이환명 대한약리학회 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3

Tetrahydrobiopterin (BH4), an essential cofactor for nitric oxide synthase (NOS) activity, is known to play important roles in modulating both NO and superoxide production during vascular diseases such as atherosclerosis. However, the role of BH4 in functions of vascular smooth muscle cells is not fully known. In this study, we tested the effects of BH4 and dihydrobiopterin (BH2), a BH4 precursor, on migration and proliferation in response to platelet-derived growth factor-BB (PDGF-BB) in rat aortic smooth muscle cells (RASMCs). Cell migration and proliferation were measured using a Boyden chamber and a 5-bromo-2'-deoxyuridine incorporation assay, respectively, and these results were confirmed with an ex vivo aortic sprout assay. Cell viability was examined by 2,3-bis [2-methoxy- 4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide assays. BH4 and BH2 decreased PDGF-BB- induced cell migration and proliferation in a dose-dependent manner. The inhibition of cell migration and proliferation by BH4 and BH2 was not affected by pretreatment with NG-nitro-L-arginine methyl ester, a NOS inhibitor. Moreover, the sprout outgrowth formation of aortic rings induced by PDGF-BB was inhibited by BH4 and BH2. Cell viability was not inhibited by BH4 and BH2 treatment. The present results suggest that BH4 and BH2 may inhibit PDGF-stimulated RASMC migration and proliferation via the NOS-independent pathway. Therefore, BH4 and its derivative could be useful for the development of a candidate molecule with an NO-independent anti-atherosclerotic function.

Controlled pH- and glucose-responsive drug release behavior of cationic chitosan based nano-composite hydrogels by using graphene oxide as drug nanocarrier

Xiaowen Zhao,Xue Zou,Lin Ye 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.49 No.-

To realize tight control of hyperglycemia for diabetic patients, cationic chitosan (HTCC) based nanocompositehydrogels were prepared by using graphene oxide (GO) as nano-carrier for the model drug(bovine serum albumin, BSA). BSA intercalated into the layers of GO and the intercalation process wasmainly driven by the mutual electrostatic interaction. By introducing GO-BSA, a more compact GOcenterednetwork structure formed for the hydrogel. Compared with HTCC/BSA, HTCC/2.0wt%GO-BSAhydrogels exhibited a more distinct pH-/glucose-sensitivity and a much lower initial burst release, whichwas attributed to the compact structure and strong interactions among HTCC, GO and BSA in the hydrogelsystem

Tetrahydrobiopterin Inhibits PDGF-stimulated Migration and Proliferation in Rat Aortic Smooth Muscle Cells via the Nitric Oxide Synthase-independent Pathway

Jiang, Xiaowen,Kim, Bo-Kyung,Lin, Haiyue,Lee, Chang-Kwon,Kim, Jung-Hwan,Kang, Hyun,Lee, Pil-Young,Jung, Seung-Hyo,Lee, Hwan-Myung,Won, Kyung-Jong The Korean Society of Pharmacology 2010 The Korean Journal of Physiology & Pharmacology Vol.14 No.3

Tetrahydrobiopterin (BH4), an essential cofactor for nitric oxide synthase (NOS) activity, is known to play important roles in modulating both NO and superoxide production during vascular diseases such as atherosclerosis. However, the role of BH4 in functions of vascular smooth muscle cells is not fully known. In this study, we tested the effects of BH4 and dihydrobiopterin (BH2), a BH4 precursor, on migration and proliferation in response to platelet-derived growth factor-BB (PDGF-BB) in rat aortic smooth muscle cells (RASMCs). Cell migration and proliferation were measured using a Boyden chamber and a 5-bromo-2'-deoxyuridine incorporation assay, respectively, and these results were confirmed with an ex vivo aortic sprout assay. Cell viability was examined by 2,3-bis [2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxanilide assays. BH4 and BH2 decreased PDGF-BBinduced cell migration and proliferation in a dose-dependent manner. The inhibition of cell migration and proliferation by BH4 and BH2 was not affected by pretreatment with $N^G$-nitro-L-arginine methyl ester, a NOS inhibitor. Moreover, the sprout outgrowth formation of aortic rings induced by PDGF-BB was inhibited by BH4 and BH2. Cell viability was not inhibited by BH4 and BH2 treatment. The present results suggest that BH4 and BH2 may inhibit PDGF-stimulated RASMC migration and proliferation via the NOS-independent pathway. Therefore, BH4 and its derivative could be useful for the development of a candidate molecule with an NO-independent anti-atherosclerotic function.

Super-strong and anti-tearing poly(vinyl alcohol)/graphene oxide nano-composite hydrogels fabricated by formation of multiple crosslinking bonding network structure

Jinlong Cao,Xiaowen Zhao,Lin Ye 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.112 No.-

As load-bearing material in biomedical-applications as cartilage replacement, artificial meniscus and tendons,etc., poly(vinyl alcohol) (PVA)/graphene oxide (GO)-tannic acid (TA) nano-composite hydrogelswith multiple-crosslinking network were fabricated by establishing freezing/thawing-annealing-swellingmethod. By TA anchoring, PVA molecules were grafted onto GO surface efficiently, and strong interfacialinteraction led to exfoliation and uniform distribution of GO in matrix. By introducing annealing process,the crystallinity and crystallite size of PVA increased and introducing GO-TA led to more perfect and densercrystalline structure, while physical crosslinking network centered on GO-TA and hydrogen bondabundantcrystalline phase formed, resulting in increasing crosslinking density of hydrogel. By furtherswelling in CaCl2 aqueous solution, hydroxyl-Ca2+ coordination formed, and multiple-crosslinking networkwas constructed with high crosslinking density. The tensile strength and fracture toughness of compositehydrogel were remarkably improved, reaching 14.38 MPa/27.93 MJ/m3, approximately 11-/26-foldhigher than those of neat PVA hydrogel, while tearing strength was significantly enhanced, attributed tohigh energy dissipation through unzipping multiple interactions.

The influences of process parameters on the preliminary roll-forging process of the AISI-1045 automobile front axle beam

Wuhao Zhuang,Lin Hua,Xiaowen Wang,Yanxiong Liu,Liying Dong,Heping Dai 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.2

Roll-forging process is widely used to fabricate the automobile front axle beams. The reasonable selection of the process parameters is significant to ensure the quality and improve the property of the automobile front axle beam owing to the complexity and importance of the preliminary roll-forging process. This paper developed a 3D Finite element (FE) model of the preliminary roll-forging process of the AISI-1045 automobile front axle beam under the Deform-3D platform. A corresponding experiment is subsequently conducted. Good agreement between the experimental and simulative results validates the employed model. Based on this dependable FE model, the influences of initial temperature of billet T 0 , rotational speed of rolls n and friction factor m on the geometric dimension, temperature inhomogeneity, deformation inhomogeneity, and roll-forging load are investigated comprehensively. The results can provide reliable guidelines for the selection of process parameters.

Fatty Acid Biosynthesis in Eukaryotic Photosynthetic Microalgae: Identification of a Microsomal Delta 12 Desaturase in Chlamydomonas reinhardtii

Xiaoyuan Chi,Xiaowen Zhang,Xiangyu Guan,Ling Ding,Youxun Li,Mingqing Wang,Hanzhi Lin,Song Qin 한국미생물학회 2008 The journal of microbiology Vol.46 No.2

Polyunsaturated fatty acids (PUFAs) are important components of infant and adult nutrition because they serve as structural elements of cell membranes. Fatty acid desaturases are responsible for the insertion of double bonds into pre-formed fatty acid chains in reactions that require oxygen and reducing equivalents. In this study, the genome-wide characterization of the fatty acid desaturases from seven eukaryotic photosynthetic microalgae was undertaken according to the conserved histidine-rich motifs and phylogenetic profiles. Analysis of these genomes provided insight into the origin and evolution of the pathway of fatty acid biosynthesis in eukaryotic plants. In addition, the candidate enzyme from Chlamydomonas reinhardtii with the highest similarity to the microsomal Δ12 desaturase of Chlorella vulgaris was isolated, and its function was verified by heterologous expression in yeast (Saccharomyces cerevisiae).

Structure and biocompatibility of highly oriented poly(lactic acid) film produced by biaxial solid hot stretching

Zhengqiu Li,Lin Ye,Xiaowen Zhao,Phil Coates,Fin Caton-Rose,Michael Martyn 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.52 No.-

Highly oriented long-chain-branched poly(lactic acid) (LCB-PLA)film was fabricated through biaxial hotstretching in solid state. Compared with neat PLA, more homogeneousfilm with higher draw ratio can beobtained for LCB-PLA. With increasing draw ratio, the long period, lamellae thickness and grain size ofLCB-PLA decreased, while the crystallinity increased. For LCB-PLA with draw ratio of 6*6, the tensilestrength and elongation at break can reach up to 208 6 MPa and 85% respectively. After drawing, theincreasing content ofCH3 and C¼O group on the surface of LCB-PLAfilm was beneficial for celladhesion and growth on it.

Preparation and drug release behavior of pH-responsive bovine serum albumin-loaded chitosan microspheres

Xue Zou,Xiaowen Zhao,Lin Ye,Qi Wang,Hao Li 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1

A novel inverse emulsion-ionic crosslinking approach was adopted to prepare pH-responsive chitosanmicrospheres and effect of preparation technique, such as chitosan concentration, emulsifierconcentration, water/oil ratio and homogenization speed, on the morphology and property of themicrospheres were investigated. The chitosan microspheres with spherical in shape, smooth surface,high productivity and promising pH responsiveness were obtained. On this basis, bovine serum albumin(BSA)-loaded chitosan microspheres was prepared to test the drug release behavior in buffers withdifferent pH values. BSA-chitosan microspheres with particle sizes in range from 5 to 10mmreleased BSAslowly in neutral medium and released rapidly in acidic medium, and exhibited obviously pH-responsiverelease behavior. Korsmeyer–Peppas model can be used for describing BSA release kinetics from chitosanmicrospheres, which corresponded with a Fickian release behavior, and the diffusion through theswelling of chitosan microspheres was the main factor in controlling BSA release.

Rhaponticin suppresses the hypoxia-induced factor-1 alpha-mediated aggressive phenotype of tongue squamous cell carcinoma

Wu Yuan,Wan Xiaowen,Shao Yisen,Wang Wei,Huang Wenquan,Zhu Jiajun,Jiang Lin 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive.We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia.The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α.Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α. Background Emerging evidence suggests that rhaponticin, a stilbene monomeric compound isolated from North China rhubarb, has been shown to exhibit significant biological activity against tumors. However, the anticancer effects and mechanisms of rhaponticin in tongue squamous cell carcinoma (TSCC) remain elusive. Objective We investigated the changes of migration and invasion abilities and EMT progression of TSCC cells treated with different concentrations of rhaponticin under hypoxia, as well as the possible mechanisms, in order to initially explore the effects of rhaponticin on the biological characteristics of TSCC cells under hypoxia. Results The number of cell migration and invasion was prominently increased, E-cadherin protein was down-regulated, and N-cadherin and HIF-1α protein expression was elevated under hypoxia. Rhaponticin intervention strikingly prevented the increased abilities of migration and invasion and EMT of TSCC cells under hypoxia. This was followed by further validation finding that rhaponticin indeed leads to reduced HIF-1α post-transcriptional activity. Mechanistically, rhaponticin may bind to aryl-hydrocarbon nuclear translocator (ARNT) domain of HIF-1α. Conclusions Rhaponticin repressed the invasion and migration abilities and EMT process of TSCC cells under a hypoxic environment in vitro by targeted suppression of HIF-1α.

Single cell gap polymer-stabilized blue-phase transflective LCDs using internal nanowire grid polarizer

Hong-Qing Cui,Zhi-Cheng Ye,Wei Hu,XiaoWen Lin,T.C. Chung,Tean-Sen Jen,Yan-Qing Lu 한국정보디스플레이학회 2011 Journal of information display Vol.12 No.3

Optically isotropic liquid crystal (LC) mixture such as blue-phase LC and nanostructured LC composites exhibit the advantages of fast response time, high contrast ratio and wide-viewing angle due to the induced birefringence along the horizontal electric field. Utilizing this mixture, a novel single cell gap in-plane switching-type polymer-stabilized blue-phase transflective liquid crystal display by embedding the nanowire grid polarizer as a polarization-dependent reflective polarizer in the R region is proposed. This device can be used as a normal black mode without any quarter-wave plate or patterned in-cell phase retarder. Moreover, the transmittance is identical to the reflectance so that it will be suitable for single gamma driving. Detailed electro-optic performances, such as voltage-dependent light efficiency and viewing angle of the proposed device configuration, are investigated.