Nematicidal and egg‐hatching inhibition activities of Kaempferia galanga rhizome‐derived materials and constituents toward root‐knot nematode (Meloidogyne incognita)

Tae‐Kyun Hong,Kym‐Na‐Ra Hwang,Jun‐Ran Kim,Young‐Joon Ahn 한국응용곤충학회 2009 한국응용곤충학회 학술대회논문집 Vol.2009 No.10

The toxicity of Kaempferia galanga rhizome materials and constituents against Meloidogyne incognita second‐stage juveniles (J2) and eggs were examined. The active principles of K. galanga rhizome were identified as the phenylpropanoids ethyl (E)‐cinnamate (EC, 1) and ethyl (E)‐p‐methoxycinnamate (EMC, 2) by spectroscopic analysis. Results were compared with those of carbofuran, fosthiazate, and metam‐sodium. In direct‐contact mortality bioassay, EC (LC50, 0.037 mg/ml) was the most toxic constituent, followed by EMC (0.041 mg/ml). EC was more effective than carbofuran (LC50, 0.092 mg/ml) but less active than fosthiazate (0.002 mg/ml). EC, egg hatch was inhibited 100, 93, and 87% at 125, 62.5, and 31.25 μg/ml, respectively. EMC caused 100, 81, and 75% inhibition of egg hatch at 125, 62.5, and 31.25 μg/ml, respectively. The inhibition of two phenylpropanoids were similar or more inhibition to that of either carbofuran or metam‐sodium but was lower than that of fosthiazate. In contact + fumigant mortality bioassay, EC and EMC treatments resulted in 86 and c 73% mortality at 0.5 and 0.125 mg/g soil, respectively. The lethality of these phenylpropanoids was almost similar to that of either carbofuran or metam‐sodium but was lower than that of fosthiazate. In vapor‐phase mortality bioassay, EC and EMC were more effective in closed container than open containers, indicating that the mode of delivery of these compounds was, in part, a result of vapor action. K. galanga rhizome‐derived materials, merit further study as potential nematicides and hatching inhibitors for the control of M. incognita populations.

전자공학교육과 실험교과내용에 관한 연구

홍봉식,김태균,오승엽,김환우,권오석 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(A)

The currenty offered experiment courses are completely reconsidered and reconfigured. The whole subjects are divided into 10 seperate courses, each of which should represent either basic or advanced stages of differing fields of the electronic engineering. It has been aimed that the courses form an elaborate hierachy so that they are minimally redundant but linked to one another in their fringes. Also, it has been pursued that the new courses should not fall obsolete in at last next five years exploring the maximum possibilities of the currently available equipments.

각종 동물 설유두의 형태학적인 관찰

김홍선,김무강,김원식,이영호,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

The lingual papillae of the mouse, the rabbit, and the cat were observed by light microscope and scanning electron microscope The results were followings 1. The filiform papillae of the mouse were classified to typical filiform papillae, conical filiform papillae with 2 branched tips, and general conical filiform papillae. The outmost layers of the filiform papillae of the mouse keratinted well, middle layer of the anterior portion of the papillae appeaered a lot of small basophilic granules. The fungiform papillae existed between the filiform papillae with the peripheral folds of epithelia. A vallate papilla existed on the posterior portion of conical filiform papillae and their shape was lenticuiar. 2. The filiform papillae of the rabbit revealed various type (5 types). Keratin layer of the papillae existed vertical to long axis of the papillae on the posterior portion of the papillae. Stratified squamous epithelium on the anterior portion of the papillae existed with a few keratin layers and the intercellular materials of strong PAS positive reaction appeared between the these cells. The fungiform papillae of the rabbit existed between the filiform papillae and had a few peripheral folds. The foliate papillae of the rabbit existed on the lateral portion of tongue root, consisted of the paralleled folds. Taste buds located in the mid-lateral portion of the papillae concentrically. 3. The filiform papillae on the anterior portion of the cat tongue shaped anterior and posterior projection, the filiform palillae on the middle portion had a projections of columnar shape, which papillae surface appeared as onion surface. The conical filiform papillae on the posterior portion had many folds on the lower portion of the papillae. The fungiform papillae of the cat existed between the filiforr papillae with a columnar shaped projectionand proturded from the surface. The vallate papillae of the cat existed between the conical filiform papillae and their shape was typical form of vallate papillae.

상추(Lactuca sativa L.)의 ACAT 억제 Diterpenoid, Phytol : 식용 식물자원으로부터 활성물질의 탐색 Development of Biologically Active Compound from Edible Plant Sources

장태오,방면호,송명종,홍윤희,김지영,정대균,배동근,권병목,김영국,이현선,김인호,백남인 한국농화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.1

고양이의 子宮支配神經의 電氣生理學 硏究

林泰均,羅重烈,洪性鳳 고려대학교 의과대학 1986 고려대 의대 잡지 Vol.23 No.1

A method was described for recording in vivo evoked potential of afferent fibers supplying the cat's uterus from the whole dorsal roots between T11 and S4 spinal segments. 1. The evoked potentials were recorded from T12, T13, L1, L7, S1, S2, and S3 segments of the spinal dorsal roots 71-hen the uterus was stimulated electrically with the intensity of 50V, duration of stimuli of 1 ms, and the frequency of stimuli of 5Hz. 2. Three types of the evoked potentials mere observed from the above dorsal roots. 1) A-β fibers from TI3 and S2 segments had the conduction volocity ranging from 24~37m/sec to 27~46m/sec. These fibers mere seemed to conduct touch and pressure sense from the uterus to the spinal cord. 2) A-β fibers from the dorsal root segments of T12, 13, L1, 7, & S1, 2, 3, had the conduction velocity ranging from 2.7m/sec to 31.6m/sec and seemed to conduct the pain and temperature sense. 3) C1 fibers from T12 dorsal root segments had the conduction velocity ranging from 1.5 m/sec to 2.2m/sec. These fibers were presumed to conduct the pair and temperature sense from the uterus. 3. The compound action potential evoked by stimulation of the uterus usually had three or four peaks, conducted at 1.5~2.2m/sec, 2.8~31.6 m/sec, and 23.5~45.5 m/sec respectively, and were likely to correspond to the fast afferent, middle, and slow afferent fibers respectively. 4. It seemed that the impulses from the uterus probably conducted through myelinated, thin myelinated and unmyelinated nerve fibers. 5. The blood pressure was increased by the electric stimulation on the uturus, but the rate of increase was noted to diminish as the dorsal roots were cut in the order of from S4 to T11. The factor for the increased BP seemed due to the painful stimulation on the uterus.

한국성인 정상교합자와 부정교합자의 연조직 측모에 관한 두부방사선 계측학적 비교연구

강홍구,윤태호,황성,정호균 중앙대부설한국의과학연구소 1991 한국의과학 Vol.23 No.4

Rat 전지 상완의 근섬유형 구성에 대한 연구

정명래,김홍선,신태균 충남대학교 의과대학 지역사회의학연구소 1987 충남의대잡지 Vol.14 No.2

This study was to determine the fiber-type populations and calculate the cross-sectional areas in each fiber-type in the muscle of the brachium of the rat. For 20 rats, the flexors (brachialis and biceps brachii) and extensors (triceps brachii) of the elbow taken from dissection. The fibers were classfied histochemically as fast-twitch oxidative glycolytic fiber(FOG), fast-twitch glycolytic fiber (FG), or slow-twitch oxidative fiber(SO), and their populations were determined. The cross-sectional areas of each type muscle fibers were calculated with microcomputer image analyzer. The results were as follows ; I. The variations in fiber-type population of various muscles with which their positions were observed as well as those reported in ot4er studies. 2. In both extensor and flexor groups, the deep region of the muscles was consisted of higher populaions of SO fibers, while more superficial regions of the same muscles were more FG fibers. 3. The percentage of SO fibers were larger in the extensors than that of flexors. 4. Except the SO fibers, the cross-sectional areas of FOG and FG fibers in the extensors were larger than that of flexors.

Nicotine이 발생중인 鷄胎의 體重 成長에 미치는 영향

金武剛,金弘善,柳時潤,申台均,李永浩,趙聲煥 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.2

The authors studies the effects of Nicotine on the Body weight growth of the developmental chick embryo. On the sixth day of incubation, 0.41mg, 0.82mg and 1.65mg of Nicotine were injected into the chorioallantoic sac of each egg of three groups and one group was injected physiological saline solution as a control, and the eggs were broken for weighting the body weight in every other day from the twelfth day to eighteenth day of incubation. In an attempt to analyze the growth of the body weights, growth formula of Zimmerman and relative growth formula of Huxley were applied. The following results were obtained. 1. The growth of chick embryo boby weight was affected as inhibiting by the Nicotine. The inhibiting effects were in proportion to the injected Nicotine dosage. 2. The growth quantity of the body weight injected 1.65mg was almost half weight of the control group body weight. 3. Growth rate was decreased continuously with tine until 18th day in the 1.65mg injected group but it was increased in 0.41mg and 0.82mg injected group with time lapse from 12th day to 18th day, and 0.41mg injected group was more increased than 0.82mg injected group. 4. Specific growth rate decreased in the all of 0.41mg, 0.82mg and 1.65mg injected group and in the decrease degree 1.65mg and 0.82mg injected group ware severe and 0.41mg injected group was slight. 5. Growth centers of growth gradient were 12th day in the 0.41mg injected group and 18th day in the 0.82mg and 1.65mg injected groups. 6. Relative growth rate was also decreased proportionally in related with injected Nicotine dosage.

치환된 안트라센 유도체들의 효과적인 합성에 관한 연구

김학원,김태균,홍수동 경희대학교 환경연구소 1998 環境硏究 論文集 Vol.8 No.-

Efficient syntheses of several 9-substituted anthracene derivatives have been one. Compounds 5, 6, 7 have been prepared from 10-monoallylated anthrone. Compound 8, 9, 10, 11, 12 have been synthesized from anthrone by the DCC coupling reaction with several acids or the base mediated coupling reaction with acyl chlorides. Compounds 14 and 15 have been prepared from the Witting reaction of 9-anthryl carboxaldehyde. 9-Substituted anthracene derivatives might be useful for environmental use and functional polymeric materials.

Cisplatin과 Tobramycin이 白鼠 Corti器에 미치는 영향에 關한 電子 顯微鏡的 硏究 : Ultrastructural Study

김동환,신태균,김무강,김홍선,김원식 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.1

In order to study the morphological changes of Corti’s organ in the rat treated with cisplatin and tobramycin, thirty-three rats, weighing between 200∼250g, were used, and divided into 4 groups, i.e., 3 rats were used for control, and 30 rats were used for 3 experimental groups. Each experimental group was divided into cisplatin-treated, tobramycin-treated, and both cisplatin and tobramycin treated group with 10 rats. Rats were injected with a single dose of 7.7㎎/㎏ of cisplatin intraperitoneally and 50㎎/㎏/day of tobramycin for 2 days intramuscularly, respectively or both. Treated rats were sacrificed on the 3rd and 5th day after the first injection. Results were as follows; 1. Cisplatin-treated group: Outer hair cells of Corti’s organ were degenerated with the formation of vacuoles and appearance of lysosomes, however, inner hair cells showed mild vacuolization. 2. Tobramycin-treated group: NO pathological changes were found in Corti’s organ. 3. Combined cisplatin and tobramycin treated group: Outer and inner hair cells showed vacuolization, appearance of lysosomes, myelin figures and multivesicular body. There were also multivesicular body in the inner phalangeal cell and myelin figure in the Reissner’s membrance. 4. Degeneration of Corti’s organ in the combined treated group was more sever than those of the single cisplatin-treated or tobramycin-treated group. 5. NO pathological differences of Corti’s organ in duration of postinjection were manifested in any groups. It can be concluded that cisplatin induces toxicity to inner ear and cisplatin enhance toxicity more in continued treated with tobramycin.