ZNF509S1 downregulates PUMA by inhibiting p53K382 acetylation and p53-DNA binding

Jeon, B.N.,Yoon, J.H.,Han, D.,Kim, M.K.,Kim, Y.,Choi, S.H.,Song, J.,Kim, K.S.,Kim, K.,Hur, M.W. Elsevier Science 2017 Biochimica et biophysica acta. Gene regulatory mec Vol.1860 No.9

Expression of the POK family protein ZNF509L, and -its S1 isoform, is induced by p53 upon exposure to genotoxic stress. Due to alternative splicing of the ZNF509 primary transcript, ZNF509S1 lacks the 6 zinc-fingers and C-terminus of ZNF509L, resulting in only one zinc-finger. ZNF509L and -S1 inhibit cell proliferation by activating p21/CDKN1A and RB transcription, respectively. When cells are exposed to severe DNA damage, p53 activates PUMA (p53-upregulated modulator of apoptosis) transcription. Interestingly, apoptosis due to transcriptional activation of PUMA by p53 is attenuated by ZNF509S1. Thus we investigated the molecular mechanism(s) underlying the transcriptional attenuation and anti-apoptotic effects of ZNF509S1. We show that ZNF509S1 modulation of p53 activity is important in PUMA gene transcription by modulating post-translational modification of p53 by p300. ZNF509S1 directly interacts with p53 and inhibits p300-mediated acetylation of p53 lysine K382, with deacetylation of p53 K382 leading to decreased DNA binding at the p53 response element 1 of the PUMA promoter. ZNF509S1 may play a role not only in cell cycle arrest, by activating RB expression, but also in rescuing cells from apoptotic death by repressing PUMA expression in cells exposed to severe DNA damage.

CBD 방법에 의한 CdS 박막의 성장과 광전도 특성

황광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdS 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 550℃로 열처리한 시료의 경우 X-선 회절무늬로 부터 외삽법에 의해 a_。와 c_。는 각각 4.1364 Å과 6.7129 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.35㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 150 K 까지는 압전산란에 의하여, 150 K 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdS thin films were grown on creamic substrate using a chemical bath deposition method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdS polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdS samples annealed in N_2 gas at 550℃ it was found hexagonal structure whose lattice constants a_o and c_o were 4.1364 Å and 6.7120Å, respectively. Its grain size was about 0.35 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33K and 150K and by polar optical scattering at temperature range of 150K and 293K. We measured also spectral response, sensitivity (γ), maximum allowable power dissipation and response time on these samples.

Chemical Bath Deposition 방법으로 제작한 CdSe 박막의 특성

홍광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdSe 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 450℃로 열처리한 시료가 X-선 회절무늬로 부터 외삽법에 의해 a_o와 c_o 는 각각 4.302 Å과 7.014 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.3㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 200 K 까지는 압전산란에 의하여, 200 k 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdSe thin films were grown on creamic substrate using a chemical bath deposition (CBD) method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdSe polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdSe samples annealed in N_2 gas at 450℃ it was found hexagonal structure whose lattice parameters a_o and c_o were 4.302 Å and 7.014 Å, respectively. It grain size was about 0.3 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33 K and 200 K, and by polar optical potical scattering at temperature range of 200 K and 293 K. We measured also spectral response, sensitivily (γ), maximum allowable power dissipation and response time on these samples.

대두박 수준을 달리한 사료내 미생물 PHYTASE 의 첨가가 잉어의 성장 및 인 배설량에 미치는 영향

김광석(K . S . Kim),김정대(J . D . Kim),송재성(J . S . Song),정관식(K . S . Jeong),우영배(Y . B . Woo) 한국영양사료학회 1996 韓國營養飼料學會誌 Vol.20 No.5

Synthesis and Thermoelectric Properties of Ce1−z Pr z Fe4−x Co x Sb12 Skutterudites

Song, K. M.,Shin, D. K.,Jang, K. W.,Choi, S. M.,Lee, S.,Seo, W. S.,Kim, I. H. Springer Science + Business Media 2017 Journal of electronic materials Vol.46 No.5

<P>p-Type Ce1-z Pr (z) Fe4-x Co (x) Sb-12 skutterudites were prepared by encapsulated melting, quenching, annealing, and hot pressing. While the skutterudite phase was successfully synthesized, a small amount of the secondary phase (FeSb2) was observed. According to the scanning electron microscope analysis, (Ce,Pr)Sb-2 phases were also observed for Co-substituted specimens (x = 0.5). The electrical conductivity decreased with increasing temperature, implying a degenerate semiconductor behavior, and also decreased with increasing Co contents. All specimens showed p-type characteristics having positive signs of the Hall coefficient and the Seebeck coefficient. The Seebeck coefficient increased with increasing temperature and reached a maximum value at 823 K. The power factor (PF) increased with decreasing Co content and Ce0.75Pr0.25 Fe4Sb12 showed a peak value of PF = 3.2 mW m(-1) K-2 at 823 K. The electronic thermal conductivity decreased with increasing Co contents and the lattice thermal conductivity decreased with decreasing Ce and Co contents at high temperature. The thermal conductivity increased at temperatures above 623 K due to bipolar conduction. The dimensionless figurea of pound merit (ZT) showed a maximum value of ZT = 0.84 at 823 K for Ce0.25Pr0.75Fe4Sb12.</P>

Search for the H Dibaryon in (K^-,K^+) Reactions

Aoki, S.,Chung, K.S.,Park, I.G.,Song, J. S. 慶尙大學校 기초과학연구소 1990 基礎科學硏究所報 Vol.6 No.-

We have studied (K^-,K^+) reactions from an emulsion target. The S=-2H dibaryon has been searched for by the analysis of the K^+ momentum spectrum together with emulsion data. No evidence of H production was observed in the mass range of 1.90-2.16 GeV/C^2. Upper limits for the production cross section of the H are (0.2-0.6)% of that for the quasifree≡^- production at the 90% confidence level.

Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

K-LAN 각 PSDN 연결을 위한 gateway machine의 구현에 관한 연구

송병권(B.K.Song),변준범(J.B.Byun),이광휘(K.H.Lee),안순신(S.S.An),최성수(S.S.Choi),신영석(Y.S.Shin),정광수(K.S.Chung),최양희(Y.H.Choi) 대한전자공학회 1986 대한전자공학회 학술대회 Vol.1986 No.6

염료공장의 톨루이딘 슬폰산 공정폐수와 종합폐수의 생물학적 처리

송승구,주승팔,이민규,박태주 한국화학공학회 1988 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.26 No.2

염료공장의 고농도 유기성 폐수인 Toluidine Sulfonic Acid(TSA) 공정폐수의 희석처리도 실험과 TSA 공정폐수와 공장전체 폐수를 혼합한 종합폐수의 처리도 실험을 행하였다. TSA 공정폐수의 응집처리는 pH가 6.5일 때 황산알루미늄 300㎎/ℓ 주입으로 약 40%의 COD 제거 효과를 나타내었다. TSA 공정폐수와 종합폐수를 완전혼합 활성슬럿지 공법으로 처리한 결과, 폭기시간 86시간 이상, F/M비 0.074㎏BOD/㎏MLSS/day 이하에서 68% 및 88% 이상의 COD 및 BOD 제거율을 얻었으며, 이 실험조건에서 동력학적 계수, k, K_s, Y, K_d의 값은 각각 0.174-0.453day^(-1), 68.8-152.6㎎/ℓ, 0.445-0.653 및 0.0022-0.0075day^(-1)의 범위로 나타났다. An laboratory-scale experiment was carried out to determine the characteristics and treatability of toluidine sulfonic acid (TSA) process wastewater and combined wastewater in a dye plant. Chemical treatment with aluminium sulfate, 300 ㎎/ℓ at pH 6.5 showed about 40% COD removal efficiency. With a completely-mixed-activated-sludge (CMAS) unit, the removal efficiencies of BOD_5 and COD were achieved more than 88% and 66%, respectively, at aeration time longer than 86 hours, that corresponded to the F/M ratio below 0.074 ㎏BOD/㎏MLSS/day. Design parameters, such as k, K_s, Y and K_d applied to CMAS mathematical equations were found as the range of 0.174-0.452 day^(-1), 68.8-152.6 ㎎/ℓ, 0.445-0.653 and 0.0022-0.0075 day^(-1), respectively.

Ciprofloxacin의 각종 감염증에 대한 임상효과에 관한 연구

강경호,송관규,유혜경,강숙경,김민자,박승철,김진호,김대원 대한감염학회 1987 감염 Vol.19 No.4

85 patients 37M and 48F, ranging in age between 18-75yrs(39±16), were treated with Ciprofloxacin at daily odse of 100㎎ bid Ⅳ (49) or 500㎎ bid p.o (30), or combined use (6) Infections included: pneumonia (19) bronchitis (15), pharyngotonilitis (1), GI tract infection (15), acute pyelonephritis (26), cystitis (2), sepsis (6), osteomyelitis (1). Severity and mode of infection were; severe 13, moderate 71, mild 1 and acute form 82, chronic 2, recurrent 1. Duration of symptoms before therapy was 4.8±4.5 days and total dose, Duration of Drug were Ⅳ: 2882±1018㎎ 7.1±2.4 dyas oral: 7167±1858㎎, 7.2±1.9 days Ⅳ and oral: 1600±858㎎/4500±1039㎎⁴±0.9/4.5±1.5 days. Isolated major organisms were; 1) pneumonia (19)-k. pneumonia (7), E. cloaceae (4), S. pneumonia(3), P. aeruginosa (2), 2) Bronchitis (15)-S. pneumonia (8), S. aureus (2), E. cloaceae (2), K. Oxytoca (2). 3) GI tract infection(15)-s. typhi (14), S. parathphi (1). 4) acute pyelonephritis (26)-E. coli (17), P. aeruginosa (4), Enterococcus (2), P. mirabilis (1). 5) cystitis (2)-E. coli (1), P. aeruginosa (1). 6) Sepsis (6)-E. coli (6), K. pneumonia (1). 7) osteomyelitis (1)-S. epidermis (1). Clinical result were resolution (64 case-75%), improvement (16-18.3%), undetermined (1-1.1%) failure (4-4.7%). Therapy result were as follows: 66 case (77.7%) were completely resolved 1 case of undetermined course in CVA with pyelonephritis. 14 case (16.5%) of partial response were 6 cases of RTI, 5 case of UTI, 5 case of thphoid fever underlying idsease; rectal cancer 1 Diabetes mellitus 4). 4 case (4.7%) of failure were 2E. coli pneunonia witn liver cirrhosis, 1 s. epidermis osteomyelitis with lymphoma and 1 enterococcus sepsis. Over all efficacy of therapy was 94.2% in 85 cases. Adverse reaction was observed in 1 patient which was fever and rash.