Salmonella Typhi, Salmonella Typhimurium 및 Salmonella Enteritidis의 항균제 감수성 (1997)

신영학,유정식,김기상,정동준,오경수,이점규,이상원,이근영,박미선,이복권,김호훈 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Site-specific mutagenesis of yeast 2-Cys peroxiredoxin improves heat or oxidative stress tolerance by enhancing its chaperone or peroxidase function

Hong, S. H.,Lee, S. S.,Chung, J. M.,Jung, H. s.,Singh, S.,Mondal, S.,Jang, H. H.,Cho, J. Y.,Bae, H. J.,Chung, B. Y. Springer Science + Business Media 2017 Protoplasma Vol.254 No.1

<P>Yeast peroxiredoxin II (yPrxII) is an antioxidant enzyme that plays a protective role against the damage caused by reactive oxygen species (ROS) in Saccharomyces cerevisiae. This enzyme consists of 196 amino acids containing 2-Cys Prx with highly conserved two active cysteine residues at positions 48 and 171. The yPrxII has dual enzymatic functions as a peroxidase and molecular chaperone. To understand the effect of additional cysteine residues on dual functions of yPrxII, S79C-yPrxII and S109C-yPrxII, the substitution of Ser with Cys residue at 79 and 109 positions, respectively, was generated. S109C-yPrxII and S79C-yPrxII showed 3.7- and 2.7-fold higher chaperone and peroxidase activity, respectively, than the wild type (WT). The improvement in enzyme activity was found to be closely associated with structural changes in proteins. S109C-yPrxII had increased beta-sheet in its secondary structure and formed high-molecular-weight (HMW) as well as low-molecular-weight (LMW) complexes, but S79C-yPrxII formed only LMW complexes. HMW complexes predominantly exhibited a chaperone function, and LMW complexes showed a peroxidase function. In addition, transgenic yeast cells over-expressing Cys-substituted yPrxII showed greater tolerance against heat and oxidative stress compared to WT-yPrxII.</P>

Phase II study of preoperative chemoradiotherapy (CRT) with irinotecan plus S-1 in locally advanced rectal cancer

Shin, S.J.,Kim, N.K.,Keum, K.C.,Kim, H.G.,Im, J.S.,Choi, H.J.,Baik, S.H.,Choen, J.H.,Jeung, H.C.,Rha, S.Y.,Roh, J.K.,Chung, H.C.,Ahn, J.B. Elsevier Science Publishers 2010 Radiotherapy and oncology Vol.95 No.3

Background and purpose: The aim of this study is to evaluate the efficacy and safety of preoperative radiation therapy combined with S-1 and irinotecan (SI) in LARC. Materials and methods: Patients were considered LARC if they had a T3/T4 lesion or node positive. Weekly doses of 40mg/m<SUP>2</SUP> irinotecan were intravenously administered once per week during weeks 1-5 of radiotherapy. S-1 (70mg/m<SUP>2</SUP>) was given from Monday to Friday in all weeks of radiotherapy. 3-D conformal radiotherapy was given at daily fractions of 1.8Gy for 5days for a total dose of 50.4 (45+5.4)Gy. Surgery was performed 4-6weeks following the completion of chemoradiation. Results: Between June 2006 and November 2007, 43 pts were enrolled. The stage was: cT3 24 patients, cT4 6 patients; 28 patients were cN+. Forty-one patients completed the chemoradiation and 42 patients underwent operation: a low anterior resection was performed in 36 patients, a total colectomy in 1 patient, and an abdominal perineal resection in 5 patients. T downstaging was observed in 50%; 23 N+ patients became N- (55%). The complete pathological response was observed in 9 patients (21%). The 3-year locoregional failure rate, distant failure rate, disease-free survival, and overall survival were 9.5%, 18.6%, 72.1%, and 94.3%, respectively. Only three patients experienced G3 diarrhea; one had G3 sepsis and two had septic shock. Hematological toxicity (G3-G4) was observed in five patients. Conclusions: This study demonstrated the efficacy of preoperative CRT with S-1 and irinotecan with 21% of complete response. However, prompt recognition and management of infection is needed to use it in patients with locally advanced rectal cancer.

한국잔디 수집계통들 중에서 우수계통들의 생육특성 비교

임용우(Y. W. Rim),김기용(K. Y. Kim),김맹중(M. J. Kim),성병렬(B. R. Sung),임영철(Y. C. Lim),정의수(E. S. Chung),신홍균(H. K. Shin),김용선(Y. S. Kim) 한국잡초학회·한국잔디학회 2003 Weed & Turfgrass Science Vol.17 No.2,3

2001년 수집된 한국잔디 133계통들 중에서 우수한 계통들을 선발하기위하여 밀도(품질),<br/> 피복성, 녹색기간, 내병성, 출수유무(종자수) 등의 주요특성과 그 외 생육특성들이 조사되었다. 생육특성이 우수한 6계통이 선발되었으며 그 결과를 요약하면 다음과 같다.<br/> 수집된 133계통들 중에서 가장 녹색기간이긴 계통은 J01067으로 11월초까지 녹색도를 유지하였다. J01106 및 J01129 계통들은 엽폭이 각각 1.5mm 및 2mm 정도로 매우 좁으며, 밀도가 높고, 피복성도 좋은 것으로 나타났으며, 내병성도 강하였다. J01122 계통은 들잔디 계통들 중에서 유일하게 내병성이 강한 계통으로 선발되었다. J01128 계통은 엽폭이 3.2mm로 중엽에 속하였으며, 대비품종인 Sunburst 와 비슷하였다. 이 계통의 경우도J01106, J01129 계통들과 마찬가지로 밀도가 높고 내병성이 강한 것으로 나타났다.<br/> 수집된 133계통들의 형태적 분류를 위하여 5가지의 생육특성을 사용하여 크게 세가지 군집<br/> 으로 분류하였으며, 그 중에서 대비품종으로 사용된 6품종들과 선발된 우수 6계통의 분류는<br/> 다음과 같다. 제 1군집에 속하는 품종 및 계통은 Belare, Meyer, 안양중지(Anyang-jungji), J01067, J01112 등이 포함되었으며, 제 2군집으로는 S-94, J01105가 포함되었고, 제 3군집에는 Sunburst, 건희(Konhee), J01106, J01128, J01129 등이 포함되었다. Growth characteristics such as density(quality), covering speed, green period, disease resistance, heading existence(number of seeds) and other characteristics were examined for selection of superior lines among the 133 zoysiagrass lines collected in 2001. Six superior lines were selected and the results were summarized as follows.<br/> Superior line, J01067 was longest for green period among the 133 zoysiagrasses and stayed green until the beginning of November. Leaf width of J01106 and J01129 lines was very narrow as 1.5㎜ and 2㎜, respectively and superior for density(quality), covering speed and disease resistance. J01122 line was selected for strongest disease resistance among the lines of Zoysia japonica. Leaf width of J01128 was 3.2㎜ showing midium type and similar to standard cultivar, Sunburst. This line also showed higher density and strong disease resistance like J01106 and J01129.<br/> Five growth characteristics for morphological classification of 133 zoysiagrass lines<br/> was used and divided into 3 cluster groups. Of 133 lines, 6 standard cultivars and 6 superior lines were classified as follows. First cluster group contained Belare, Meyer, Anyang-jungji, J01067, J01122, and second group contained S-94 and J01105, and third group contained Sunburst, Konhee, J01106, J01128 and J01129.<br/> <br/>

세관 양광주 방전에서 플라즈마 확산의 완전 해

김동준,정종문,김정현,황하청,정재윤,조윤희,임현교,구제환,최은하,조광섭,Jin, D.J.,Jeong, J.M.,Kim, J.H.,Hwang, H.C.,Chung, J.Y.,Cho, Y.H.,Lim, H.K.,Koo, J.H.,Choi, E.H.,Cho, G.S. 한국진공학회 2010 Applied Science and Convergence Technology Vol.19 No.1

관경이 수 mm인 세관 램프 내부에서 플라즈마의 확산을 조사하기 위하여 이극성(ambipolar) 확산방정식을 해하였다. 반경 방향의 확산에 의한 유리관 벽에서의 플라즈마 소멸 특성시간은 $\tau_r\;=\;(r_0/2.4)^2/D_a$로 주어진다. 반경 $r_0{\sim}1\;mm$이고 이극성 확산계수 $D_a{\sim}0.01\;m^2/s$ 이면, $\tau_r{\sim}17\;{\mu}s$이다. 이는 램프의 교류전원 구동에서 플라즈마를 유지하기 위한 구동 최소 주파수 ~30 kHz에 해당한다. 고전압이 인가되는 전극부에 발생한 고밀도의 플라즈마가 양광주로 확산되는 특성시간은 $\tau_z{\sim}0.1\;s$이다. 고밀도 플라즈마 경계에서의 시간에 대한 확산속도는 $t{\sim}10^{-6}\;s$일 때 $u_D{\sim}10^2\;m/s$이고, $t{\sim}10^{-3}\;s$이면 그 속도는 $u_D{\sim}1\;m/s$로 느려진다. 따라서 램프 길이 ~1 m에 대하여 전극부에서 생성된 고밀도 플라즈마가 양광주 전체로 확산되는 시간은 수 초가 걸린다. The ambipolar diffusion equation has been solved in a fine-tube lamp of a few mm in diameter. In the diffusion of radial direction, the plasma diffuses and vanishes away at the glass wall by recombination with the characteristic time of plasma loss is given by $\tau_r\;=\;(r_0/2.4)^2/D_a$. With the radius $r_0{\sim}1\;mm$ and the ambipolar diffusion coefficient $D_a{\sim}0.01\;m^2/s$, the vanishing time is calculated $\tau_r{\sim}10\;{\mu}s$ which corresponds to the least value of frequency 30 kHz for the sustaining the plasma in the operation of high voltage AC-power. In the diffusion of longitudinal z-direction, a high density plasma generated at the area of a high voltage electrode, diffuses into the positive column with the characteristic time $\tau_z{\sim}0.1\;s$. The plasma diffusion velocity at the boundary of high density plasma is $u_D{\sim}10^2\;m/s$ at the time $t{\sim}10^{-6}$ s and the diffusion velocity becomes slow as $u_D{\sim}1\;m/s$ at $t{\sim}10^{-3}\;s$. Therefore, for the long lamp of 1 m, it takes about several seconds for the high density plasma at the area of electrode to diffuse through the whole positive column space.

A phase II study of S-1 monotherapy administered for 2 weeks of a 3-week cycle in advanced gastric cancer patients with poor performance status

Jeung, H-C,Rha, S Y,Shin, S J,Ahn, J B,Noh, S H,Roh, J K,Chung, H C Nature Publishing Group 2007 The British journal of cancer Vol.97 No.4

<P>Systemic chemotherapy for gastric cancer is often associated with treatment-related toxicity, which is particularly severe in patients with a poor performance status. In this paper, we describe the first study to evaluate S-1 monotherapy as an option for advanced gastric cancer patients who are not candidates for combination chemotherapy due to poor clinical condition. Fifty-two patients with Eastern Cooperative Oncology Group (ECOG) performance scale 2–3, whose general condition had made use of combination chemotherapy impossible, were enrolled. S-1 was administered to 30 patients as second- or third-line therapy. The initial dose of S-1 was 35 mg m<SUP>−2</SUP>, administered b.i.d for 14 days every 3 weeks. With a median follow-up period of 33 weeks, the median progression-free survival, and overall survival were 11 weeks (95% CI, 8–14) and 33 weeks (95% CI, 19–47), respectively. The overall 1-year survival rate was 29% by intent-to-treat analysis. The overall response rate was 12% (95% CI, 3–21), and the percentage of stable disease was 35%, resulting in the disease control rate of 47% (95% CI, 32–60). Significant drug-related toxicity included grade 3 diarrhoea (14%), anorexia (14%), fatigue (10%), neutropenia (10%), and leucopenia (6%). In conclusion, this study indicated the modest activity of S-1 in gastric cancer patients with poor performance status.</P>

서울대학교 保健大學院 博士課程 敎育프로그램의 評價的 硏究

정인숙,박태진,김성옥,배은영,박은옥,김정순,문 용 서울大學校保健大學院 1997 國民保健硏究所硏究論叢 Vol.7 No.2

The doctoral program of school of Public Health in Seoul National University was established in 1976 and about 100 students have graduated or completed the course. Even though there have been several studies on evaluating the educational programs of the school, both master's and doctorate's since early 1980s, this study was carried out to identify needs of students, their performance and perceived achievements, unmet needs and barriers in achieving their educational goals only on doctoral graduates. The study was done by the students of doctoral program taking 'evaluative research in public health services' course as a practice under the direction of the teaching staff. Out of 91 eligibles 51 persons responded to the mail and/or telephone interview, and the results obtained are as followings: 1) The goal of the doctoral program of the Public Health, SNU that was to produce competent public health specialists furnished with full abilities of performong research, education and administration(service)has not been changed since 1976 as was set on the program establishment. 2) The objective of enrollment to the program stated by the subjecs varied considerably; majority of them(47%) for expanding scope of public health sceience, for advanced degree(39%), for contribution to the progress of public health sceience(20%), for better job(20%) and for pure academic interest(2%). 3) The needs of students by area of concern, for instance research, theaching and administration, each classified into several performance is viewed as the proportion accomplished out of needs, which are quantified by 5-point Likert scale, most of the graduates indicated that they have accomplished their needs in 83-92% for research abilities, 75-92% for teaching sbilities and 72-96% for administration abilities, the average performance being the highes in research. 5) The barriers that hindered their performance were the school facilities, such as library(72.5%), deficient funds for research(39.2%), insufficient students' room(33.3%) and deficient laboratory supplies(25.5%). 6) The barrier related to student's characteristics were insufficient study time for part-time student(56.9%), deficient study motivation(45.1%), tendency to take easy curriculum(29.4%) and addmittence of less qualificated students(19.6%). 7) The barrier related to professor's characteristics were deficiency of self-development endeavour(62.7%), lack of professor majoring in some particular fields of public health(39.2%), infrequent collaborative researches/activities with students (17.6%), and absolutely deficient number of professors that are necessary for the program(17.6%). In general, however, the graduates appered to be satisfied in terms of what they have accomplished through the current educational program, which have been revised by the school from time to time to cope with the student's as well as community's demand.

COMPARISON OF THE COMBUSTION CHARACTERISTICS BETWEEN S.I. ENGINE AND R.I. ENGINE

Chung, S.S.,Ha, J.Y.,Park, J.S.,Kim, K.J.,Yeom, J.K. The Korean Society of Automotive Engineers 2007 International journal of automotive technology Vol.8 No.1

This experimental study was carried out to obtain both low emissions and high thermal efficiency by rapid bulk combustion. Two kinds of experiments were conducted to obtain fundamental data on the operation of a RI engine by a radical ignition method. First, the basic experiments were conducted to confirm rapid bulk combustion by using a radical ignition method in a constant volume chamber (CVC). In this experiment, the combustion velocity was much higher than that of a conventional method. Next, to investigate the desirable condition of engine operation using radical ignition, an applied experiment was conducted in an actual engine based on the basic experiment results obtained from CVC condition. A sub-chamber-type diesel engine was reconstructed using a SPI type engine with controlled injection duration and spark timing, and finally, converted to a RI engine. In this study, the operation characteristics of the RI engine were examined according to the sub-chamber's specifications such as the sub-chamber volume and the diameter and number of passage holes. These experimental results showed that the RI engine operated successfully and was affected by the ratio of the passage hole area to the sub-chamber volume.

COMPARISON OF THE COMBUSTION CHARACTERISTICS BETWEEN S.I. ENGINE AND R.I. ENGINE

S. S. CHUNG,J. Y. HA,J. S. PARK,K. J. KIM,J. K. YEOM 한국자동차공학회 2007 International journal of automotive technology Vol.8 No.1

This experimental study was carried out to obtain both low emissions and high thermal efficiency by rapid bulk combustion. Two kinds of experiments were conducted to obtain fundamental data on the operation of a RI engine by a radical ignition method. First, the basic experiments were conducted to confirm rapid bulk combustion by using a radical ignition method in a constant volume chamber (CVC). In this experiment, the combustion velocity was much higher than that of a conventional method. Next, to investigate the desirable condition of engine operation using radical ignition, an applied experiment was conducted in an actual engine based on the basic experiment results obtained from CVC condition. A sub-chamber-type diesel engine was reconstructed using a SPI type engine with controlled injection duration and spark timing, and finally, converted to a RI engine. In this study, the operation characteristics of the RI engine were examined according to the sub-chamber’s specifications such as the sub-chamber olume and the diameter and number of passage holes. These experimental results showed that the RI engine operated successfully and was affected by the ratio of the passage hole area to the sub-chamber volume.

[디젤엔진부문] 이종연료 층상분사를 이용한 디젤엔진의 NOx와 soot 동시 저감에 관한 연구

강병무(B.M. Kang),김종률(J.Y. Kim),이태원(T.W. Lee),정성식(S.S Chung),하종률(J.Y. Ha) 한국자동차공학회 2000 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

<br/> ~~.A\-%7}t'--c:j-~ 2000\15:. ~;>jI~t:1J~<br/> 2000-03-2041<br/> 01<br/> 3S. 91 ~ ~A~~<br/> A<br/> L.s:.<br/> OI<br/> Ho~<br/> c:"~.n. C::>C>c r2. C:::>L ;<br/> C/~2!!~~ NOx$ soot ~AI AI~OU ~~ eF?<br/> 7J'!!-';'-, 7,J'6-it(%ottJI-o:}jj! tJI~~), 01I11~(~~ ~~tJI-O:}), '\J'\J-6j, "6J-'6-iH%ottJI-o:}jj!)<br/> A Study on the Simultaneous Reduction of :>iOx and Soot<br/> with Dual Fuel Stratified Injection System in a Diesel Engine<br/> B. 1\1. Kang, ]. Y. Kim(Graduated School of Dong- A L'niv'>, T. W. Lee(Changwon CQUage), S. S. Chung, ]. Y. Ha(Dong-A Univ,)<br/> ABSTRACT<br/> To investigate the method of the simultaneous reduction of Soot and NOx, we developed a new conception for dual fuel stratified injection system in a Diesel Engine.<br/> Some parts of the injection system, ie. nozzle holder, delivery vale. were remodeled to inject diesel and methanol in order from one injector. The Quantity of diesel and methanol was controled precisely by micrometers that were mounted at control lack of injection pump.<br/> The real injection Quantity ratio of dual fuel was certificated by volumetric ratio at injection Quantity experiment. We could confirm the capability that soot and NOx simultaneously were reduced by diesel-methanol stratified injection from the results of combustion and exhaust characteristic.<br/>