http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
(±)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향
오은경,박형건,배기현,최옥진,최은경,최창근,한진희,정태천 한국환경독성학회 2002 환경독성보건학회지 Vol.17 No.4
Effects of (±)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of (±) -camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with (±)-camphor at the doses used. Administration o(±)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that (±)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 213 in male ICR mice.
Park, Hayng-Mi,Shin, Sang-Hyun,Ko, Jong-Min,Yi, Gi-Hwan,Nam, Min-Hee,Chung, Young-Soo,Chung, Won-Bok,Lee, Jai-Heon,Park, Seong-Whan Korean Society of Life Science 2004 생명과학회지 Vol.14 No.1
이소플라본의 함량이 매우 높은 것으로 알려진 국내 콩품종 신팔달로부터 2개의 유전자 IFS1 (SinIFS1)과 IFS2(SinIFS2)가 클로닝되었다. 유전자의 염기서열을 밝힌 후, 기존에 알려진 콩과의 다른 IFS 유전자들과 유전자 염기서열의 유사성을 비교 분석하였다. 유전자 SinIFS1은 전체 1,828bp의 nucleotide와 521개의 아미노산으로 이루어져 있었고 SinIFS2의 경우, 1912bp의 nucleotide와 521의 아미노산으로 이루어져 있었다. 두 유전자 모두 cytochrome P45O superfamily의 일원이었고, 상응하는 conserve된 motif들을 가지고 있었다. 콩과의 다른 식물에서 클로닝된 IFS들과의 염기서열비교에서는 매우 높은 염기서열 유사성(98% 이상)이 관측되었다. 유전자의 발현과 유발에 관한 노던분석 실험 결과, 무처리구로 사용한 암처리보다 모두 유발된 유전자의 발현을 나타났는데, 특히 곰팡이 elicitor 처리구의 경우, 무처리보다 6배 이상의 유전자 유발을 보였다. 그 다음으로는 자외선 처리가 높은 유전자 발현 유발효과를 나타내었고, 그 다음으로 저온과 명처리순으로 유발효과를 나타내었다. Two genes, SinIFS1 and SinIFS2 from Korean soybean cultivar, Sinpaldalkong known as one of isoflavonerich cultivars, were cloned with PCR and degenerate primers. The sequences of two genes were analyzed with previously reported IFS genes of leguminous plants and their expression pattern in various environmental conditions was surveyed. The genomic clone of SinIFS1 contained 1,828bp nucleotides and encoded a polypeptide of 521 amino acids, and 1912bp nucleotides and a polypeptide of 521 amino acids for SinIFS2. Both genes included several conserved motifs, oxygen binding and activation (A/G-G-X-E/D-T-T/S), ERR triad (E...R....R), and heme binding (F-X-X-G-X-R-X-C-X-G) domain, which are typical in any member of cytochrome P45O superfamily. Very high sequence homology (>98%) was observed in the comparison with other IFSs of legumes. In the northern blot analysis to check the expression and increase of SinIFS1 to various environmental renditions (low temperature, light, dark, UV, and fungal elicitor), the most significant induction, more than 6 times of transcript level compared to the dark treatment as a control, was observed from the fungal elicitor treatment. The next up-regulated expression was from UV treatment (4${\times}$), low temperature and light conditions.
대장관 내압 상승으로 유발된 허혈성 대장염 15예의 분석
박수헌,김명훈,김기범,조세현,차상복,문성배,배상수,한원희,최규용,조환석 대한소화기내시경학회 1996 Clinical Endoscopy Vol.16 No.6
It was suggested that temporary vascular insufficiency within the wall of the colon causes iachemic colitis. So, motivated by this fact, we studied and analyzed 15 cases of iachemic colitis, which were confirmed by the diagnostic criteria of Nagasako(1982), through 32 months from January, 1993 to August, 1995 at Our Lady of Mercy Hospital, Incheon. The results were as follows: 1) Average age was 51.3 years(17 to 79 years), 10 of 15 cases were above 50 years and the rest were below 50 years. The ratio of men to women was 7:8. 2) The causes were 8 cases of enema, 3 cases' of taking laxative drugs, 3 cases of strain, and 1 case of having colonofiberscopy. 3) The symptoms were 15 cases of abdominal pain(100%), 15 cases of bloody stool (100%), and 7 cases of diarrhea(47%). The signs were 13 cases of abdominal tenderness(87%), 3 cases of fever(20%), and 11 cases of leukocytosis(above 10.0000/㎣)(73 %). 4) The involved sites were 13 cases in the sigpnoid colon(87%), 10 cases in the descending colon(67%), and 9 cases in the rectum(60%). 5) The colonafiberscopc findings were 15 cases of diffuse hyperemia(100%), 13 cases of erosions and hemorrhage(87%), 13 cased of edema(87%). 9 cases of exudate (60%), and 6 cases of ulcer(40%). The longitudinlal hyperemia or shallow ulceration, which are characteristic of colonofiberscopic findings of ischemic colitis, were found in 11 eases(73%). After a week, the follow-up endoscqpy showed that most of lesions were improved. And after another wek, sill cases had recovered. 6) All cases were improved by NPO and fluid therapy; the period of fluid therapy was 5.89 days. 7) The pathological findings were 11 cases of chronic ulceration(79%) and 1 cases each of erosion, superficial hemorrhage, melansis coli(7%). And only a patient was not biopsy.