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- 저자 : Park Chang-H (검색결과 1,133 건)
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Park, Y-H,Kim, S-U,Kwon, T-H,Kim, J-M,Song, I-S,Shin, H-J,Lee, B-K,Bang, D-H,Lee, S-J,Lee, D-S,Chang, K-T,Kim, B-Y,Yu, D-Y Macmillan Publishers Limited 2016 Oncogene Vol.35 No.27
<P>The current study was carried out to define the involvement of Peroxiredoxin (Prx) II in progression of hepatocellular carcinoma (HCC) and the underlying molecular mechanism(s). Expression and function of Prx II in HCC was determined using H-ras(G12V)-transformed HCC cells (H-ras(G12V)-HCC cells) and the tumor livers from H-ras(G12V)-transgenic (Tg) mice and HCC patients. Prx II was upregulated in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg mouse tumor livers, the expression pattern of which highly similar to that of forkhead Box M1 (FoxM1). Moreover, either knockdown of FoxM1 or site-directed mutagenesis of FoxM1-binding site of Prx II promoter significantly reduced Prx II levels in H-ras(G12V)-HCC cells, indicating FoxM1 as a direct transcription factor of Prx II in HCC. Interestingly, the null mutation of Prx II markedly decreased the number and size of tumors in H-ras(G12V)-Tg livers. Consistent with this, knockdown of Prx II in H-ras(G12V)-HCC cells reduced the expression of cyclin D1, cell proliferation, anchorage-independent growth and tumor formation in athymic nude mice, whereas overexpression of Prx II increased or aggravated the tumor phenotypes. Importantly, the expression of Prx II was correlated with that of FoxM1 in HCC patients. The activation of extracellular signal-related kinase (ERK) pathway and the expression of FoxM1 and cyclin D1 were highly dependent on Prx II in H-ras(G12V)-HCC cells and H-ras(G12V)-Tg livers. Prx II is FoxM1-dependently- expressed antioxidant in HCC and function as an enhancer of Ras(G12V) oncogenic potential in hepatic tumorigenesis through activation of ERK/FoxM1/cyclin D1 cascade.</P>
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Park, C.S.,Yi, Y.J.,Kim, M.Y.,Chang, Y.J.,Lee, S.H.,Jin, D.I 충남대학교 형질전환복제돼지연구센터 2004 논문집 Vol. No.8
This study was to investigate the effects of fertilization time and culture medium of pig oocytes matured in-vitro by liquid boar sperm. The sperm rich fraction (30∼60 ml) was slowly cooled to room temperature (20∼23℃) by 2 h after collection. Semen was transferred into 15 ml tubes, centrifuged at room temperature for 10 min 800×g, and the supernatant solution was poured off. The concentrated sperm was resuspended with 5 ml of the LEN diluent to provide 1.0×10^(9) sperm/ml at room temperature. The resuspended semen was cooled in a refrigerator to 4℃. The medium used for oocyte maturation was TCM-199 supplemented with 26.19 mM sodium bicarbonate, 0.9 mM sodium pyruvate, 10μg/ml insulin, 2μg/ml vitamin B_(12), 25 mM HEPES, 10μg/ml bovine apotransferrin, 150μM cysteamine, 10IU/ml PMSG, 10 IU/ml hCG, 10 ng/ml EGF, 0.4% BSA, 75μg/ml sodium penicillin G, 50μg/ml streptomycin sulfate and 10% pFF. After about 22 h of culture, oocytes were cultured without cysteamine and hormones for 22 h at 38.5℃, 5% CO₂in air. Oocytes were inseminated with liquid boar sperm stored at 4℃ for 2 days after collection. Oocytes were coincubated for 1, 3, 6 and 9 h in 500 μl mTBM fertilization media with 1.0×10^(6) sperm/ml concentration, respectively. Thereafter, oocytes were transferred into 500 μl NCSU-23, HEPES buffered NCSU-23, PZM-3 and PZM-4 culture media, respectively, for further culture of 6, 48 and 144 h. The rates of sperm penetration and male pronuclear formation were higher in the fertilization times for 6 and 9 h than in those for 1 and 3 h. The rates of cleaved oocytes were higher in the fertilization times for 6 and 9 h (85.0 and 84.6%) than in those for 1 and 3 h (61.1 and 76.8%). The percentage of blastocyst formation from the cleaved oocytes was highest in the fertilization time for 6 h (33.6%) than in that for 1, 3 and 9 h (11.4, 23.0 and 29.6%). Mean cell numbers per blastocyst were 32.9, 27.6, 26.3 and 24.4 in the fertilization times for 6, 9, 3 and 1 h, respectively. The rate of blastocyst from the cleaved oocytes and the number of cells per blastocyst were higher in HEPES buffered NCSU-23 culture medium than in NCSU-23, PZM-3 and PZM-4 culture media. In conclusion, we found out that liquid boar sperm stored at 4℃ could be used for in-vitro fertilization of pig oocytes matured in-vitro. Also, we recommend the coincubation time of 6 h in 500 μl TBM fertilization medium with 1×10^(6) sperm/ml concentration and the HEPES buffered NCSU-23 culture medium for in-vitro fertilization of pig oocytes matured in-vitro.
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Nguyen, T.H.,Than, V.T.,Thanh, H.D.,Nguyen, V.Q.,Nguyen, K.H.,Nguyen, D.T.,Park, J.H.,Chung, I.S.,Jeong, D.G.,Chang, K.T.,Oh, T.K.,Kim, W. Pergamon Press 2015 Comparative immunology, microbiology and infectiou Vol.42 No.-
In Vietnam, highly pathogenic avian influenza (HPAI), such as that caused by H5N1 viruses, is the most highly contagious infectious disease that has been affecting domestic poultry in recent years. Vietnam might be an evolutionary hotspot and a potential source of globally pandemic strains. However, few studies have reported viruses circulating in the south-central region of Vietnam. In the present study, 47 H5N1-positive samples were collected from both vaccinated and unvaccinated poultry farms in the South Central Coast region of Vietnam during 2013-2014, and their genetic diversity was analyzed. A common sequence motif for HPAI virus was identified at HA-cleavage sites in all samples: either RERRRKR/G (clades 2.3.2.1c and 2.3.2.1a) or REGRRKKR/G (clade 1.1.2). Phylogenetic analysis of HA genes identified three clades of HPAI H5N1: 1.1.2 (n=1), 2.3.2.1a (n=1), and 2.3.2.1c (n=45). The phylogenetic analysis indicated that these Vietnamese clades may have evolved from Chinese and Cambodian virus clades isolated in 2012-2013 but are less closely related to the clades detected from the Tyva Republic, Bulgaria, Mongolia, Japan, and Korea in 2009-2011. Detection of the coexistence of virus clades 2.3.2.1 and the very virulent 1.1.2 in the south-central regions suggests their local importance and highlights concerns regarding their spread, both northwards and southwards, as well as the potential for reassortment. The obtained data highlight the importance of regular identification of viral evolution and the development and use of region-specific vaccines.
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Park, M. Y.,Kim, Y. J.,Hwang, S. H.,Kim, H. H.,Lee, E. Y.,Jeong, S. H.,Chang, C. L. American Society for Microbiology 2009 Journal of clinical microbiology Vol.47 No.2
<P>We evaluated a new immunochromatographic assay (ICA) using mouse monoclonal anti-MPT64 antibody for rapid discrimination between Mycobacterium tuberculosis and nontuberculous mycobacteria in clinical isolates. A study with mycobacteria and other organisms showed excellent sensitivity (approximately equal 99%) and specificity (100%) and an appropriate detection limit (10(5) CFU/ml) when tested with M. tuberculosis H37Rv. This ICA can simplify the identification of M. tuberculosis in clinical laboratories.</P>
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박구부,장판형 경상대학교 축산진흥연구소 1982 畜産振興硏究所報 Vol.9 No.1
In order to determine the changes in pH, water holding capacity(WHC) and tenderness of chicken meat during the postmortem storage, the breast and leg muscles were obtained from Rhode Island Red Chicken of 2, 6 and 18 months old send stored at 4℃ for 7 days postmortem. The results obtained were as follows: 1) The pH was not significantly affected by the difference of muscle position and chicken ages. However, the pH was rapidly decreased in the early storage period up to 8 hours afterslaughtering, but this trend was reversed at 48 hrs and the pH change was not recognized from 48 hours up to 168 hours of postmortem storage. 2) The WHG of the muscles of 2-month chicken was significanttly higher than that . of 18-month chicken. The WHC was not affected by the muscle positions but storage period caused considerable changes in the WHC up to 8 hours postslaughtering. 3) The tenderness of the muscles of 18-month chicken was significantly lower than that of 2-, and 6-month chicken. The tenderness was not significantly different between muscle positions but significantly decreased up to 8 hours postslaughtering and increased gradually thereafter.
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오리고기에 있어서 저장기간에 따른 보수성과 지방산 조성변화
박구부,김영환,장판형 경상대학교 축산진흥연구소 1986 畜産振興硏究所報 Vol.13 No.1
This experiment was carried out to investigate changes in WHC, lipid contents and fatty acid composition from duck muscles(9 months) during postmortem storage. Experimental animals were normally slaughted to produce sample from leg, breast and gizzard muscles. And the samples were stored for 0, 8, 48, 96 and 168hrs at 4℃. Fatty acid compositions were analysed by GLC. The results obtained were as follows: WHC of duck muscles at right after slaugutering 71.7-74.0%, respectively. WHC decreased rapidly at 8hrs, but increased only slightly at 48hrs and increased a little at 168hrs postmortem storage. As the storage time passed the means of total lipid contents were 1-3% duck muscles, respectively. Fatty acid was identified to ten from myristic acid to arachidonic acid in duck muscles by GLC. The highest composition of fatty acids was oleic acid(38-44%) in duck muscles. The next higher compositions of fatty acid were linoleic(26-27%) and palmitic acid(19-22%) in breast and leg muscles. Although saturated fatty acids increased as the storage time passed, unsaturated fatty acids relatively decreased in experimental animals, the composition of unsaturated fatty acids was higher than that of saturated fatty acids.
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Park, Y.,Jang, S. K.,Park, J. H.,Yang, S. Y.,Chung, H.,Han, Y.,Chang, Y. S.,Choi, I. G.,Yeo, H. Springer Science + Business Media 2017 Journal of wood science Vol.63 No.6
<P>The effects of the combined treatment of drying and heat treatment using superheated steam (SHS) were studied relative to the changes of the major chemical components in larch wood. The green lumber was dried and heat-treated in SHS conditions of 250 A degrees C and 0.5 MPa for 18 h, and the relative percentage contents of sugars, lignin, and extractives were investigated and compared with the relative percentage contents in the lumber heat-treated in hot air conditions of 250 A degrees C and atmospheric pressure for 18 h. After both heat treatment methods, the relative percentage contents of xylan, mannan, galactan, and arabinan were greatly decreased, whereas that of the Klason lignin was increased, additionally that of glucan and extractives remained almost unchanged. Lignin may bind with furan compounds decomposed from hemicellulose following heat treatment, thus contributing to the increase in the apparent relative percentage contents of the Klason lignin. In addition, the condensate collected in the condenser after combined drying and heat treatment using SHS was investigated qualitatively and quantitatively by high-performance liquid chromatography (HPLC). A large amount of furfural and acetic acid decomposed from hemicellulose was detected and some sugar components composed of cellulose and hemicellulose were detected in the liquid condensate.</P>
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소아 쭈쭈가무시병환자 28명의 임상상 및 유병기간에 따른 간접면역형광항체가의 변동상
박병규,오영균,윤희상,염명걸,유한욱,김재형,강환섭,김익상,장우현,김승환 대한감염학회 1993 감염 Vol.25 No.2
During the autumn of 1990 , we had investigated the clinical featuresw and followed the change in the direct immunofluorescent antibody(IFA) titers in 28 children with scrub typhus who resided in or near Chinju, Korea. All the children except one were diagnosed by the peak IFA titers of 1:160 or higher. The results were as follows: 1) The clinical features of our children were not different, to a large degree, from those of adults ever described in Korea literatures. But the frequence rates of skin rash, eschar and conjunctival injection were higher, those of proteinuria and hematuria were lower than those in adults and the characters of rash in our children were also quite different(faces were frequently the first site of eruption and even palms and soles were involved occasionally, initial bright red color of rash was changed into brown color during the acute phase). 2) IFA titers rose rapidly during the 2nd or 3rd week of illness and then formed plateau. When we suggest IFA titers of 1:180 or higher as the diagnostic criteria, the seropositivity of our children was 5/7(75%) in the 1st week and over 95% in the 2nd through 5th week of illness, and suggest IFA titers of 1:160 or higher,then the seropositivityin the 1st, 2nd and 3rd through 5th week were 3/7(43%), 23/31(74%) and over 95%,respectively. 3) IFA titers in 6-8 months after disease onset were 1:80(13 children) or 1:20( 7 children), and in one and half year (our longest follow-up period), 1:20 (3 children) showing that IFA lasts longer than one and half year after sickness. 4) From the above data (3) and the fact that majority of cases of scrub typhus occur in the autumn season in Korea, IFA titers of 1:40-1:160 should be used as the diagnostic criteria in order to distinguish between the present and past infections.
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