Overexpression of Zoysia ZjCIGR1 gene confers cold stress resistance to zoysiagrass

Yang‑Ji Kim,Dae‑Hwa Yang,Mi‑Young Park,Hyeon‑Jin Sun,Pill‑Soon Song,Hong‑Gyu Kang,Seok‑Cheol Suh,Yong‑Eok Lee,이효연 한국식물생명공학회 2020 Plant biotechnology reports Vol.14 No.1

Zoysia japonica Steud. is a warm-season lawn grass popular in Korea and elsewhere. They are cultivated in many places such as river banks, roadside, and play grounds. However, there still is a disadvantage of frequent mowing, and the grass grows poorly under the chilly conditions. To develop a grass variety that circumvents these drawbacks, we cloned the chitininducible gibberellins-responsive1 gene (CIGR1) from zoysiagrass. The full length of the ZjCIGR1 (Zj; Zoysia japonica Steud.) gene was obtained by 5′/3′ RACE PCR and the phylogenetic tree showed that it belonged to the CIGR1-subgroup in the PAT1-group of GRAS protein family. Expression of the ZjCIGR1 in wild-type plants was confirmed in roots, meristems, leaves, and flowers, especially high in the flowers. The transgenic zoysiagrass was confirmed by PCR using gene-specific primers, phosphinothricin-acetyl-transferase (PAT) strip test, and Southern blot analysis. ZjCIGR1-overexpressing plants acquired tolerance to cold stress displaying morphological phenotypes characteristic of stress resistance. In addition, in the transformants, expression of the ZjCIGR1 as well as cold-regulated (COR) gene was increased compared to the wild-type plants under cold stress condition. These results suggest that ZjCIGR1 gene is an important candidate for regulating cold stress resistance.

전신마취중 에피네프린사용으로 발생되는 부정맥

박선규,진홍용,양홍석 중앙대부설한국의과학연구소 1985 한국의과학 Vol.17 No.3

Cone-beam CT를 이용한 제II급 부정교합 아동의 상기도 형태에 관한 3차원적 연구

홍지숙,김대성,오경민,김윤지,이규홍,박양호 대한치과교정학회 2010 대한치과교정학회지 Vol.40 No.3

Objective: The aim of this study was to evaluate the volumes and areas of the upper airways in children with Class II malocclusion, using three dimensional cone-beam computed tomography (CBCT) and to compare the volumetric and cross-sectional measurements and cephalometric variables to investigate possible relationships between the upper airway and facial morphology. Methods: CBCT scans were obtained from 37 subjects (17 boys and 20 girls; average age, 11.02 years). The upper airway volumes and areas were measured, and compared with cephalometric variables. Results: The area of the PNS-posterior plane (SPP) was significantly smaller in the Class II malocclusion group (p < 0.05). Also, the volumetric and cross-sectional measurements were lower in Class II than in Class I malocclusion groups, although the differences were not significant between the two groups (p > 0.05). The Class II malocclusion group showed significantly smaller values of PFH, mandibular body length, pog to N perp and showed larger values of FMA, ANB, and facial convexity than the Class I malocclusion group. The volume of the upper airway in front of PNS point (WN) showed negative correlation with ANB (p < 0.05). Conclusions: The Class II malocclusion group had a narrower upper airway associated with a decreased posterior facial height and a divergent growth pattern than the Class I malocclusion group. 성장기 아동의 상기도 협착은 두개안면 성장에 영향을 미칠 수 있다. 본 연구는 3차원 cone-beam computed tomography (CBCT)를 이용하여 제II급 부정교합 아동의 상기도 부피 및 단면적 계측치와 측모두부계측치를 비교하여 상기도와 안면 형태와의 연관성을 알아보고자 하였다. 37명의 아동(남자 17명, 여자 20명, 평균 연령 11.02세)의 CBCT를 촬영하였다. 연구 결과, 상기도의 PNS-posterior plane에서의 단면적(S_(PP))은 제II급 부정교합군에서 유의하게 작았다 (p < 0.05). 상기도 부피 계측치는 제II급 부정교합군에서 제I급 부정교합군보다 작았으나, 두 군 간 통계적으로 유의한 차이는 없었다. 제II급 부정교합군은 후안모고경(PFH), mandibular body length (p < 0.05), Pog to N perp (p < 0.01)에서 제I급 부정교합군보다 유의하게 작은 값을 보였고, FMA (p < 0.05), ANB, facial convexity (p < 0.01)는 유의하게 큰 값을 보였다. 제II급 부정교합군의 PNS 전방의 상기도 부피(W_(N))는 ANB difference 각도와 음의 상관관계를 보였다 (p < 0.05). 제II급 부정교합 아동은 상대적으로 좁은 상기도 부피를 가지며 이에 따른 감소된 후안모고경(PFH)과 하악골의 divergent한 안면 성장 양상을 보였다.

계분으로부터 살모넬라에 감염된 가금류의 신속한 진단

박홍양 동물자원연구센터 1999 1999년도 동물자원연구센터 학술심포지움 Vol.1999 No.-

우리의 건강과 깊은 관계가 있는 유해미생물중 하나인 살모넬라균은 최근 심각한 사회문제로 대두되고 있다. 가금류인 닭의 경우 살모넬라가 주로 장내에 번식하고 있어 도살시 계육뿐만 아니라 계분과 함께 배출되어 산란된 난에까지 오염이 이어지고 있다. 더불어 육계, 산란계로부터 조기에 살모넬라 감염여부를 파악하는 것이 중요한데 현재의 검출법 중 하나인 단순배양법의 경우 시간이 오래걸리고 많은 인력과 비용이 드는 단점이 있다. 그래서 본 연구는 살모넬라에 감염된 닭의 분을 이용해 유전공학기법의 하나인 PCR(Polymerase Chain Reaction)을 이용해서 살모넬라균 특유의 유전자의 염기 서열을 찾아 검출에 필요한 primer(S-1,S-2,S-7,S-8)를 제작하고, 이를 적절하나 조건(94 ℃에서 30초간의 denaturation, 63 ℃에서 20초간 annealing, 72 ℃에서 40초간의 extension 과정을 30번 반복)에 맞추어 실험하여 닭의 도축전에 감염여부를 신속하게 판단하여 양계농가의 경제적 이익과 국민의 건강증진에 초점을 두고 있다.

마우스의 장내 농양(intraabdominal abscess)형성에 대한 연구

박장환,정용훈,조양자,김정목,전윤홍 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

Intraabdominal abscesses (IAA) are produced microbiologically by mixed infection. And clinical treatment of IAA is limited because duration of the these diseases are prolong and the their nature is mixed infection. This study was carried out to develop a standardized IAA model used in B.fragilis. Encapsulated B.fragilis and autoclaved colonic and cecal contents produced IAA> And encapsulated B.fragilis produced IAA more than unencapsulated B. fragilis. Only E.coli was not effective to produce IAA but combination with encapsulated B.fragilis produced IAA. Abscesses were usually found in the serosal surface adherent to pancrease or colon and in the fatty tissue around the uterus. This study will subsequently support a development of IAA treatment.

측두엽에 발생된 Gliosarcoma

박양화,권익승,홍승관,문명선,신미경 대한신경외과학회 1989 Journal of Korean neurosurgical society Vol.18 No.1

저자들은 근래 좌측 측두엽에 발생된 Gliosarcoma 1예를 수술적 부분적출 및 수술후 방사선 조사로 치료한 후 일시적 증상 호전을 보이다가 수개월 내에 급속한 임상적 악화 및 종양 재증식을 치험하고 희귀한 증례이기에 보고하고저 한다. Gliosarcoma는 악성 뇌교종과 섬유육종이 혼합된 뇌종양으로써, 문헌 고찰 결과 악성 뇌교종 내의 혈관내피세포 과증식이 어떠한 알려지지 않은 기전에 의하여 악성 변화를 일으켜 섬유육종이 발생되는 경우가 대부분으로 사료된다. A case of left temporal gliosarcoma is reported. The patient was a 40-year old man who had headache, behavior change, dysphasia, and mental confusion for about 2 months duration. On brain CT, a large partially cystic mass with homogeneous enhancement was located at left temporal lobe and associated with remarkable pentumoral edema and mass effect. Angiographic tumor vascularity was faintly visualized. Surgical subtotal resection of the tumor and post-operative radiotherapy offered transient symptomatic improvement, being followed within several months by clinical deterioration and regrowth of the tumor on brain CT. Light microscopic studies of the H & E and special stained specimens showed two different components within the tumor in a mixed fashion, i.e. intermingled glioblastoma multiforme and fibrosarcoma. Electron microscopic study of the sarcomatous cells disclosed intracytoplasmic Weibel-Palade-like bodies, a specific marker of endothelial cells. The fibrosarcomatous component of gliosarcoma is believed to be originated from malignant transformation of the hyperplastic endothelial cells within the anaplastic glioma. Literature concerned with gliosarcoma is reviewed.

교류전해에칭용 에틸렌글리콜의 알루미늄박판의 부식억제 효과

박성민, 양정진, 김홍일, 김한주, 김성한, 신진식, 박수길 충북대학교 건설기술연구소 2008 충북대학교 건설기술연구소 논문집 Vol.27 No.1

In this study, additions of aluminium foil eching characteristics were compared with sulfuric acid for capacitor. sulfuric acid and ethylene glycol is used as addition, characteristics of funtional group from addition were guessed by comparing of aluminium foil eching characteristics. When sulfuric acid was added in HCI etching solution, corrosion of aluminum metal was inhibited by the chemical adsorption of sulfate ion, when ethylen glycol was added in HCI etching solution, corrosion inhibited by the chemical adsorption of OH­^ion.

Mulligan 치료법과 고유수용성 신경근 촉진법이 동결견 환자의 치료에 미친 효과

양정애,윤홍일,박현식,신영일,전범수 대한정형도수치료학회 2006 대한정형도수물리치료학회지 Vol.12 No.1

The purpose of this study was to compare the effects of Mulligan method and PNF method on the pain and limitation of range of motion in patients with frozen shoulder. The subjects of this study were 20 patients, 10(50%) males and 10(50%) females. They visited clinic for physical treatment within 6 months after onset of shoulder pain and limitation of range of motion with frozen shoulder. One group was applied with Mulligan method and other group was with PNF method . The patient were treated 5 times session weekly for 6 weeks from March 4th, 2002. And each treatment session was 15min. with physiotherapy. The pain was measured by visual analogue scale (VAS) and rage of motion (ROM) of flexion, abduction, external rotation, internal rotation were measured by goniometer. The data was analysed by paired T-test and independent T-test. The results of this study were summarized as follow : 1. The ROM of Mulligan method group increase in after treatment in comparison with ROM in before treatment, it is significant increase. Although the VAS of MMG decrease in before than after treatment, it is significant difference. 2. There is significant difference in before and after treatment of ROM of shoulder flexion, abduction, internal rotation, external rotation between PNF method group. The VAS of PMG is decreasing in before than after treatment, it is significant difference. 3. There is significant difference in before movement and after movement ROM of flexion, abduction, internal rotation, external rotation and VAS between Mulligan method session and PNF method session then the scale which measured by Mulligan method was more increased than that of PNF method. The results showed that both Mulligan method and PNF method were effective in pain reduce and ROM increase, but Mulligan method was superior to PNF method in ROM increase and pain reduce.

치과용 인상재의 점성에 관한 연구

박영준,김강연,양홍서 大韓齒科器材學會 1994 대한치과재료학회지 Vol.21 No.1

The purpose of this study was to investigate the rheological properties of a syringe type agar impression material, a zinc oxide eugenol impression paste and 6 types of elastomeric impression materials. Boiled agar impression material was cooled from 80℃ to 32℃, and its viscosity was measured during the temperature decrease at three different shearing rates. Viscosities of unmixed impression pastes were measured at three different temperature conditions and three different shearing rates. Impression materials were mixed at 23 and 30℃, and the viscosities were measured as a function of elapsed time from mixing. At 30℃ mixing condition, viscosities of condensation silicone rubber impression materials were measured with the variation of base/catalyst ratio, and viscosities of addition silicone rubber impression material with the varying amounts of retarder were measured. The results are as follow; 1. In all impression materials, viscosity values were decreased as the shear rate increased(P<0.05). 2. Viscosity of agar impression material was increased rapidly when the temperature decreased below 42℃(P<0.05). 3. Viscosities of unmixed impression materials were decreased by the increased temperature. Condensation and addition silicone rubber impression materials showed low viscosities, and polysulfide impression materials showed moderate viscosities, and polyether and zinc oxide eugenol impression materials showed much higher viscosities than other impression materials. 4. As the room temperature increased, the mixed impression materials showed significantly rapid increase of viscosities during the same lapse of time(P<0.05). Polysulfide rubber and zinc oxide eugenol impression materials retained low viscosities for longer time in the course of setting. Ramitec, polyether rubber for bite registration, attained high viscosity within short elapsed time. On the other hand, Impregum-F polyether rubber impression material showed slowed slower increase of viscosity than condensation and addition silicone rubber impression materials. 5. Viscosities of condensation silicone rubber impression material reacted sensitively with the variation of base/catalyst ratio(P<0.05). 6. Addition silicone rubber impression material mixed with 2 drops of retarder per 5 cm pastes showed no significant difference in viscosity compared with normal mixing condition(P>0.05), but 4 drop-added mix showed significant decrease in viscosity compared with normal mixing group(P<0.05).

ASEA-SKF 래들용 고알루미나 내화재의 용손성향

박홍채,이윤복,양태영,장윤식,오기동 釜山大學校生産技術硏究所 1997 生産技術硏究所論文集 Vol.53 No.-

ASEA-SKF 래들에 사용된 고알루미나 내화물의 광물 및 화학적 변화를 화학분석, 현미결관찰, X-선회절분석으로 조사하였다. 미사용 벽돌의 주요 광물상은 α-Al₂O₃, mullite, tridymite이었다. 가동면에서 SiO₂결정상이 소실되었으며 mullite와 α-Al₂O₃가 다소 감소하였고 2FeO·SiO₂와 hercynite, FeO, Cr₂O₃상이 생성되었다. 침식율은 강종에 따라 다소간 차이가 있었으며 용강 SF-45와 60분간 반응시켰을때 SK36벽돌의 침식율은 75.9%인 반면에 SK38벽돌의 침식율은 20.47%이었다. 대부분의 용손벽돌에서 가동면으로부터 내부로 감에 따라 열적 및 화학적 스폴링으로 인하여 미세균열이 존재하였다. Mineral and chemical changes of alumina refractories used in ASEA-SKF ladle were examined by chemical analysis, microscopy and X-ray diffraction method. Main minerals in unused high alumina runner bricks were α-Al₂O₃, mullite and tridymite. In working face of high alumina runner bricks, SiO₂phase was not observed, mullite and α-Al₂O₃phases more or less decreased. On the other hand, 2FeO·SiO₂, hercynite, FeO and Cr₂O₃phases were formed. Th corrosion of high alumina runner bricks showed different behavior by using various types of iron melts. Expecially, corrosivites of SK36 bricks and SK38 bricks, which immersed in SF-45 Iron melts for 60min, were 75.9% and 20.47%, respectively. Microcracks in high alumina runner bricks increased from the working face to the inside due to thermal and chemical spalling.