N/O/N 박막 다이아프레임을 이용한 광섬유 압력센서의 제작 및 그 압력특성

유양욱,김명규,박동수,김창원,김진섭,이정희,손병기 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

The 0.6 μm N/O/N triple layer, Si_(3)N_(4)(150 nm)/SiO_(2)(300 nm)/Si_(3)N_(4)(150 nm), was deposited on silicon substrate and the N/O/N thin film diaphragm was formed using anisotropic etching technique. The stress of the formed diaphragm was very small with temperature variations. Combining this diaphragm and the optical fiber, intensity-type pressure sensor was fabricated and its pressure characteristics were investigated. The relation between the optical output power, inversely proportional to the deflection of the diaphragm, and the applied pressure was almost linear in 0~77 torr range.

매설형 광섬유 Fabry-Perot 간섭형 센서의 제조 및 특성

이호일,이경진,김명규,박재희,강신원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1

Fiber-optic Fabry-Perot Interferometric(FFPI) sensor was embedded in the scaled down reinforced concrete structure and its characteristics were investigated. The intrinsic FFPI consists of two internal mirrors 11 mm apart in the optical fiber and TiO_(2) thin film was sputtered for the mirrors. The sensor showed excellent optical interference characteristics and the phase shift of the sensor output signal was proportional to the external loads applied to the structure. Three sensors at different points were multiplexed by the TDM method, so the deformation to the external loads at each point in the structure could be simultaneously monitored.

지단백과 알부민 및 이들의 변형물질이 메산지움세포 증식에 미치는 영향

손일석,이태원,박재경,김희진,조병수,임천규,김명재 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.2

목 적 : 혈청 지단백과 알부민은 그 자체로써 또는 산화나 당화 과정에 의한 변형을 거쳐 사구체 질환을 진행시킬 수 있는 것으로 알려져 있다. 그 기전은 분명하지는 않으나 메산지움세포 증식과 관련이 있는 것으로 알려져 있다. 저자들은 비교적 간단한 흡광도측정법을 이용하여 지단백과 알부민 및 그들의 변형물질들이 각각 메산지움세포 증식에 미치는 영향을 알아보고자 하였다. 방 법: 정상인의 혈청 지단백을 원심분리 하여 저밀도지단백, 고밀도지단백, 초저밀도지단백을얻고, 산화 저밀도지단백, 당화 저밀도지단백, 그리고 혈청 알부민과 당화 알부민을 각각 농도를 달리하여 배양 중인 인간 메산지움세포에 가한 후 흡광도를 측정함으로써 메산지움세포의 증식정도를 알아보았다. 양성 대조군으로 interleukin-1β를 이용하였다. 결 과: 저밀도지단백은 농도 증가시 50 μg/mL와 100 μg/mL에서 세포의 증식을 보였으며,고밀도지단백과 초저밀도지단백은 농도에 따른 유의한 변화가 없었다. 산화 저밀도지단백은5 μg/mL에서부터 세포의 증식을 초래한 후 10 μg/mL와 25 μg/mL에서도 이를 유지하였다. 당화 저밀도지단백은 농도 증가에 따라 10 μg/mL와 100 μg/mL에서 세포의 증식을 억제하였다. 알부민은 농도 500 μg/mL에서 세포 증식을 억제하다가 고농도(1,000 μg/mL)에서는 영향이 없었으며, 당화 알부민 역시 100 μg/mL의 적은 농도에서는 억제효과를 보였으나 고농도(1,000 μg/mL)에서는 오히려 증식을 일으키는 결과를 보였다. 결 론: 저농도에서 저밀도지단백과 산화 저밀도지단백은 사람 메산지움세포의 증식을 초래하였으며, 당화 저밀도지단백은 증식을 억제하였다. 알부민은 일정농도까지 사람 메산지움세포의 증식을 억제하다가 고농도에서는 영향이 없었으며, 당화 알부민도 일정농도까지 증식 억제 효과가 있다가 고농도에서는 오히려 증식을 초래하는 양상을 보였다. Background : Modified lipoproteins may be involved in nephro- and glomerulosclerosis. Diabetic nephropathy-like lesions have also been induced in a rat model by glycated and glycoxidized albumin. In cultured rat or human mesangial cells, enhanced cell proliferation and production of mesangial matrix in response to lipoproteins and their modified forms have been demonstrated by [3H]-thymidine incorporation and cell counting assays. But these methods are relatively complex and most of them have used only one or two of the lipoprotein, albumin and their modified forms. Methods : We investigated the effects of native and modifed lipoproteins, and albumin on cultured human mesangial cell proliferation using non-radioactive colorimetric method by MTS/PMS assay. Lipoproteins added were low density lipoprotein(LDL), high density lipoprotein(HDL), very low density lipoprotein(VLDL), oxidized LDL(oxidation with copper sulfate in vitro) and glycated LDL and we also used albumin, glycated albumin, and interleukin-1β as a positive control. Results : Interleukin-1β promoted the proliferation of cultured human mesangial cells up to concentration 20 ng/mL. LDL induced the proliferation of mesangial cells in a concentration-dependent manner up to concentration 100 μg/mL. HDL and VLDL had no significant proliferative effect. Oxidized LDL caused the proliferation of mesangial cells at low concentration up to concentration 25 μg/mL. Addition of glycated LDL resulted in a concentrationdependent inhibition of mesangial cells. Albumin and glycated albumin inhibited the proliferation of mesangial cells at low concentration of 100 μg/mL, but cell growth was increased at higher concentrations. Conclusion : We demonstrated the effects of the single and modified proteins on the proliferation of cultured human mesangial cell by relatively simple colorimetric method. Results were almostly identical to those of previous studies obtained by radioactive method or cell counting assay. (Korean J Nephrol 2002;21(2):266-275)

시령탕과 온비탕의 저밀도지단백의 산화 억제 효과

이재진,이태원,장현하,김희진,임천규,김명재 대한신장학회 2001 대한신장학회잡지 Vol.20 No.3

Interferometric-Type Fiber Optic Pressure Sensor using A Diaphragm and a Fiber Optic Fabry-Perot Interferometer

Myung Gyoo Kim,Jae Hee Park,Dae Hyuk Kwon,Byung Ki Sohn 대한전자공학회 1997 대한전자공학회 학술대회 Vol.1997 No.1

당뇨병 환자에서 신중 유무에 따른 자율신경기능의 차이

김명임,이태원,고용호,나학열,이계원,김희진,임천규,김명재 대한신장학회 2000 대한신장학회잡지 Vol.19 No.6

同種肝臟移植에 關한 實驗的 硏究 -豫報-

金洙泰,朴哲圭,蔣舜明,金鏞日,朴容眩,洪俊浩,吳壽明,羅成均,金晋煥 최신의학사 1970 最新醫學 Vol.13 No.4

Preliminary experiment of six auxiliary and four orthotopic canine hepatic allotransplantation was undertaken in this laboratory in 1969. Buster's and Hori' s methods and the other modifications were tried for six auxiliary and four orthopic allogenic transplantation avoiding the use of venous bypass. The one method <Fig. l-(3)> for the recipient procedure, is followed by: 1. Side to side portacava1 shunt, 2. Right hepatectomy, 3. Orthotopic allotransplantation, 4. Left hepatectomy. The two cases of survival, 10 days and 45 days respectively, were observed in the cases of auxiliary heterotopic allotransplantation, and the latter case is still alive at the time of this roport. On the 6th postoperative day, liver scanning revealed the graft is still in function in the former case. The necrosis of graft liver was noted upon autopsy on the 10th postoperative day. The liver function and C. B. C. were checked at a few days interval after transplantation in the latter case. Within 4 weeks, the liver function had become almost within normal limits, but the elevation of alkaline phosphatase is still persistent. His general condition appears to be healthy.

유전체 다이아프램을 이용한 다모드 광섬유 압력센서

김명규(Myung-Gyoo Kim),권대혁(Dae-Hyuk Kwon),김진변(Jin-Sup Kim),박재희(Jae-Hee Park),이정희(Jung-Hee Lee),손병기(Byung-Ki Sohn) 한국진공학회(ASCT) 1997 Applied Science and Convergence Technology Vol.6 No.3

실시콘 미세가공기술로 형성된 프레임 모양의 실리콘 기판에 의해 지지되는 100 ㎚-Au/30 ㎚-NiCr/150 ㎚-Si₃N₄/300㎚-SiO₂/150㎚-Si₃N₄ 광반사막을 제조하였으며, 이것을 광섬유와 결합하여 강도형 다모드 광섬유 압력센서를 제작하고 그 특성을 조사하였다. Si₃N₄/SiO₂/Si₃N₄ 다이아프램을 광반사막으로 사용하기 위하여 이 다이아프램의 뒷면에 NiCr 및 Au 박막을 각각 진공증착하여 광반사막에서의 광투과에 의한 광손실을 수 %로 감소시킬 수 있었다. 유전체 다이아프램의 상하에 각각 있는 Si₃N₄막은 KOH 수용액에 의한 실리콘 이방성 식각시 자동시각 정지층 역할을 하여 다이아프램 두께의 재현성이 우수하였다. 다이아프램의 크기가 3×3 ㎟, 4×4 ㎟ 및 5×5 ㎟인 센서는 각각 0~126.64 kPa, 0~79.98 kPa 및 0~46.66 kPa의 압력범위에서 선형적인 광출력-압력 특성을 나타내었으며, 이들 센서의 압력감도는 각각 약 20.69 nW/kPa, 26,70 nW/kPa 및 39.33 nW/kPa로서, 다이아프램의 크기가 증가할수록 압력감도도 증가하였다. An optical intensity-type pressure sensor has been fabricated by coupling multimode optical fiber with 100 ㎚-Au/30 ㎚-NiCr/150 ㎚-Si₃N₄/300 ㎚-SiO₂/150 ㎚-Si₃N₄ optical reflection layer supported by micromachined frame-shape silicon substrate, and its characteristics was investigated. For the application of Si₃N₄/SiO₂/Si₃N₄ diaphragm to the optical reflection layer of the sensor, NiCr and Au films were deposited on the backside of the diaphragm by thermal evaporation, respectively, and thus optical loss caused by transmission in the reflection layer could be decreased to a few percents. Dielectric diaphragms with uniform thickness were able to be also reproduced because top- and bottom-Si₃N₄ layer of the diaphragm could automatically stop silicon anisotropic etching. The respective pressure ranges in which the sensor showed linear optical output power-pressure characteristics were 0~126.64 kPa, 0~79.98 kPa, and 0~46.66 kPa, and the respective pressure sensitivities of the sensor were about 20.69 nW/kPa, 26.70 nW/kPa, and 39.33 nW/kPa, for the diaphragm sizes of 3×3 ㎟, 4×4 ㎟, and 5×5 ㎟, indicating that the sensitivity increases as diaphragm size increases.

Changes in Antibody Titers of Measles, Mumps, Rubella, and Hepatitis B Virus After Bone Marrow Transplantation in Korea : A Preliminary Report

Chung, Myung Sun,Park, Yoon Hee,Park, Sung Gyoo,Choi, Su Mi,Min, Woo Sung,Shin, Wan Shik,Lee, Seong Hee,Yoo, Jin Hong,Kim, Chun Choo,Choi, Jung Hyun,Kim, Yong Jae,Kim, Dong Jip 대한감염학회 1998 감염 Vol.30 No.6

목 적 : 조혈모세포 이식술 후 환자의 면역기능손상에 따라서 추가 예방접종의 시기와 필요성을 알아보기 위하여 measles, mumps, rubella 및 간염바이러스에 대한 항체가를 측정하였다. 방 법 : 20명의 환자에서 이식전과 이식후 3, 6, 9, 12개월째 혈청을 분리하여 MMR과 간염바이러스 각각의 항체가를 효소면역측정법을 이용하여 정량적으로 추적검사 하였다. 결 과 : Measles, rubella, 그리고 HBV에 대한 항체가는 이식후 일년까지는 통계학적으로 의미 있게 감소하지는 않았으나 mumps에 대한 항체가는 이식 후 6개월 후부터 급속히 감소하여 이식 전에 비해 유의한 차이를 보였다. Measles, mumps, rubella 및 간염바이러스에 대한 항체가는 이식후 감소하는 양상을 보였으나 평균값은 면역력을 가진 것으로 관찰되었다. 결 론 : 조혈모세포 이식후 1년까지는 MMR 및 간염에 대한 추가면역은 불필요한 것으로 사료되며, 필요하다면 환자의 면역상태를 개별적으로 파악하여 실시하는 것이 바람직하다. 추후 더 많은 환자수와 이의 추적기간을 연장하여 국내에서의 조혈모세포이식후 추가면역접종의 필요성 및 시기를 결정하고자 한다. Background : Bone marrow transplantation (BMT) has become a significant treatment modality for hematopoietic adn solid organ malignancy. Recipients of BMTs lose immunity to measles-mumps-rubella (MMR) and hepatitis B infections which are preventable with vaccination. There is no consensus regarding a vaccination schedule after BMT and time of vaccination is variable according to each institution. We analyzed sequential changes in antibody titers of MMR and hepatitis B during the first year after BMT in an attempt to identify the time, dose, and needs for revaccination. Methods : Total 20 patients with hematologic malignancies were studied. Serum levels of IgG antibodies of MMR and hepatitis B virus (HBV) were determined every three months post-BMT by enzyme immunoassay (EIA), chemical luminescence immunoassay (CLIA) and immunofluorescence assay (IFA). Results : IgG antibody levels of measles, rubella, HBV were 1:746, 80±85 IU/mL, 214±343 IU/L before BMT, declined to 1:633, 18±11 IU/mL, 4±6 IU/L one year after BMT, respectively. All the antibody levels were still above cut-off value for positive immunity. Mumps antibody titers were 1:62 before BMT, declined to 1:25 significantly from 6 months after BMT, but the antibody level was still above cut-off value. Conclusion : Antibody titers of MMR and hepatitis B decline during the first year after BMT, but the levels are still above cut-off value. Thus, the timing of revaccination should be after the first year post-transplantation. Long-term studies are needed to determine the optimal time for revaccination.

신증후군 환자의 혈장 Platelet Factor - 4 ( PF4 ) 와 Beta - Thromboglobulin ( β -TG )의 변화

김명재 ( Myung Jae Kim ),임천규 ( Chun Gyoo Ihm ),박충기 ( Choong Gee Park ),유영길 ( Young Kil Yoo ),한인권 ( In Kwon Han ),권오선 ( O Sun Kwon ),장영운 ( Young Woon Chang ),김이숙 ( Rhee Sook Kim ) 대한내과학회 1984 대한내과학회지 Vol.27 No.9