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      • 柑橘의 耐寒性 品種育成 및 耐寒 栽培技術에 關한 硏究

        金智文,宋鎬京,金鼎錫 충남대학교 농업과학연구소 1977 農業技術硏究報告 Vol.4 No.2

        Present studies were carried out for breeding cold resistant clones of Citrus, improving overwintering techniques of Citrus in Jeju island as well as other southern region, Result obtained were as follows 1. In the vinyl house covered with two sheets of straw mat, 12 indivuals were found as non-injury and 15 were slightly injured by leaf freeging test at -9℃ for 2 hours treatment. 2. In the condition of vinyl house covered with straw mat and viny film mulching and heat-in by sun-light, the inside temperature of vinyl house were not lowered below -3℃ and the ground temperature in vinyl house keeps above 0℃ during winter though outdoor temperature were lower by -15℃(Daejeon area). 3. The vinyl tunnel inside the vinyl house and vinyl film mulching an ground position showed greater effectiveness for preventing heat loss from house but there were no significant difference between the color of vinyl film covered the tunnel. 4. In the vertical distribution of maximum temperature in vinyl house, the upper space was slightly higher than the lower position at high temperature condition, while minimum temperature was distributed as higher as the middle position, ground surface and upper position in order at low temperature condition 5. In the horizontal distribution of temperature in vinyl house, ground and surface temperature of north side was lower than the other sides, and citrus planted within 30cm from north side wall died by cold injury and in the other side near wall appeared slight symptom of cole injury. 6. The insulating trench(30×30×30cm) packed with straw bundle installed under north wall might be effective to prevent heat loss of ground temperature. 7. In cloudy and snowy day, the temperature difference between indoor and outdoor were less, and the indoor temperature were maintained highly during night due to the effect of prevention of heat loss. 8. The highest temperature in a day was observed at about P.M. 3 both inside and outside of vinyl house and the lowest temperature was observed at about A.M. 6. The difference between the highest and lowest temperature of indoor in a sunny day was 19℃, compared with 9℃ on a cloudy or snowy day in late November. Especially, lowering of temperature in a snowy day was so less that the curve of temperature change was comparatively constant. 9. If the effective methods of citrus cultivation in vinyl house with improved clone such as hardiness, semi-dwarf and spur type are applied, it might be possible to cultivate the citrus tree safely in Jeju island as well as other southern rejion and to magnity the cultivation of citrus tree.

      • KCI등재

        I-SSR 標識子를 利用한 호도나무屬의 識別 및 類緣關係 分析

        黃錫仁,曺炅眞,李文鎬,李在善,李炳實,李郁 한국임학회 2004 한국산림과학회지 Vol.93 No.7

        DNA 표지자를 이용한 호도나무 품종의 유연관계 및 품종구분을 위하여 I-SSR 9개 표지를 이용하여 104개의 유의적인 표지가 얻어졌으며, 신품종 호도나무를 포함한 83개 국내외 품종(종)에 대해 DNA 지문에 의한 품종식별이 가능하였다. 수원 1호, 수원 2호, 왕호도는 모두 Conrord, McKinster 등 미국에서 도입된 대립성 품종과의 인공교배로 육성된 품종으로 유전적 유연관계가 상촌 4호 등 국내 선발종보다는 산성 4호×Concord의 교배종과 더 가까웠다. 국내 선발종 호도나무인 상촌 2호, 상촌 4호 및 산성 4호 이들 끼리 한 그룹을 이루고 있는데 이는 국내의 호도나무가 외국품종과 구분되는 유전적 동질성이 있음을 보여주었다. 영동은 개체선발로 육성되었으나 외국종과 교배에 의하여 육성된 교잡종들과 같은 무리에 속하였는데, 이는 영동 품종은 외국 품종의 교배에 의해 외래 유전자 일부가 유입된 것이 아닌가 추정된다. Concord×상촌 1호., Concord×상촌 2호, Concord×대부 1호가 한 무리를 이루었고 Lake×상촌 2호가 이들과 구분되는 것은 교배차대가 유전적으로 화분수보다는 모수의 영향을 더 많이 받고 있음을 보여준다. Using 104 fragments amplified from 9 I-SSR primers, a total of 83 cultivars in Juglans spp. including new cultivars used for the present study were identified. The new cultivars 'Suwon No.1', 'Suwon No.2', and 'Wanghodo' are hybrids between the domestic cultivars and the introduced cultivars such as 'Concord' and 'McKinster' bearing big nuts. Consequently, these cultivars were closer to the hybrid between 'Sansung 4×Concord' than to domestically selected cultivars such as 'Sangehon 4' from the viewpoint of genetic relationship. The domestic cultivars such as 'Sangchon 2', 'Sangchon 4', and 'Sansung 4' belonged to the same group and were genetically distinctive from the introduced cultivars. This suggests that there would be difference in genetic structure between the domestic and the introduced cultivars. The cultivar 'Yeongdong' was clustered into the group of hybrids between the domestic and the introduced cultivars although it was developed by the selection of a tree from a domestic population. This might be because the genes were introduced from the introduced cultivars into the domestic population via open-pollination. The 'Concordx×Sangchon 1', 'Concord×Sangchon 2', and 'Concord×Daebu 1' were Clustered into the same group whereas 'Lake×Sangchon 2' was clustered into the different group. This ndicates that the genetic characteristics of hybrids may be influence more strongly by the mother tree than by the father tree.

      • KCI등재후보

        Flowable 및 microfill 복합레진으로 충전된 제 5급와동에서 load cycling 전,후의 미세변연누출 비교

        강석호,김오영,오명환,조병훈,엄정문,권혁춘,손호현 大韓齒科保存學會 2002 Restorative Dentistry & Endodontics Vol.27 No.2

        Low-viscosity composite resins may produce better sealed margins than stiffer compositions (KempScholte and Davidson, 1988; Crim, 1989). Flowable composites have been recommended for use in Class V cavities but it is also controversial because of its high rates of shrinkage. On the other hand, in the study comparing elastic moduli and leakage, the microfil had the least leakage (Rundle et al. 1997). Furthermore, in the 1996 survey of the Reality Editorial Team, microfills were the clear choice for abfraction lesions. The purpose of this study was to evaluate the microleakage of 6 composite resins (2 hybrids, 2 microfills, and 2 flowable composites) with and without load cycling. Notch-shaped Class V cavities were prepared on buccal surface of 180 extracted human upper premolars on cementum margin. The teeth were randomly divided into non-load cycling group (group 1) and load cycling group(group 2) of 90 teeth each. The experimental teeth of each group were randomly divided into 6 subgroups of 15 samples. All preparations were etched, and Single bond was applied. Preparations were restored with the following materials (n=15) : hybrid composite resin [Z250(3M Dental Products Inc. St.Paul, USA), Denfil(Vericom, Ahnyang, Korea)], microfil [Heliomolar RO(Vivadent, Schaan, Liechtenstein), Micronew(Bisco Inc. Schaumburg, IL, USA)], and flowable composite[AeliteFlo(Bisco Inc. Schaumburg, IL, USA), Revolution(Kerr Corp. Orange, CA, USA)]. Teeth of group 2 were subjected to occlusal load (100N for 50,000 cycles) using chewing simulator(MTS 858 Mini Bionix Ⅱ system, MTS Systems Corp., Minn, USA). All samples were coated with nail polish 1mm short of the restoration, placed in 2% methylene blue for 24 hours, and sectioned with a diamond wheel. Enamel and dentin/cementum margins were analyzed for microleakage on a sclale of 0 (no leakage) to 3 (3/3 of wall). Results were statistically analyzed by Kruskal-Wallis One way analysis, Mann-Whitney U-test, and Student-Newmann-Keuls method. (p=0.05) Results: 1. There was significantly less microleage in enamel margins than dentinal margins of all groups. (p<0.05) 2. There was no significant difference between six composite resin in enamel margin of group 1. 3. In dentin margin of group 1, flowable composite had more microleakage than others but not of significant differences. 4. There was no significant difference between six composite resin in enamel margin of group 2. 5. In dentin margin of group 2, the microleakage were R>A=H=M>D>Z. But there was no significant differences. 6. In enamel margins, load cycling did not affect the marginal microleakage in significant degree. 7. In dentin margins, load cycling did affect the marginal microleakage only in Revolution. (p<0.05)

      • KCI등재

        ASME 및 CSA 코드에 의한 초음파 결함 크기 측정

        박문호,강석철 韓國非破壞檢査學會 1998 한국비파괴검사학회지 Vol.18 No.4

        국내 원자력발전소 가압경수로 및 가압중수로에 대한 가동중검사 수행 동안 초음파 검사로 검출된 결함을 ASME 코드에서 기술한 center line beam 방법과 CSA 코드에서 기술한 6 dB drop 방법을 이용한 결함의 깊이 방향 크기 및 길이를 측정 비교ㆍ분석하므로서 두 방법을 정확히 이해하고 결함 평가에 대한 신뢰성 향상 및 원전의 건전성을 확보하고자 한다. To record and evaluate the flaws which were found during pre-service/in-service inspection performance of nuclear power plants in Korea, the center line beam method described in ASME code and 6dB drop method stated in CSA code were used. The measured through wall dimensions and lengths by these methods were compared and analyzed in this report. With the measured data analysis, the exact understanding and use of these methods improves the reliability of flaw sizing and assures the integrity of nuclear power plant components.

      • 기성 콘크리트 파일 두부정리를 위한 자동화 장비의 개발

        이정호,김정렬,김영석,조문영,김성근 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2

        During the last few years, the use of prefabricated concrete pile has been gradually increased in many construction sites. Cutting work of the concrete pile is an important task to crush a part of pile head which is compressed with more than 800㎏/㎠, It is usually performed by a crusher and three to four skilled workers. Recent analysis results of the pile cutting work reveal that it frequently makes a lot of cracks which significantly reduce the strength of the pile and is labor-intensive work. The primary objective of this study is to propose conceptual designs for developing an automated pile cutting machine. It is anticipated that the development of the automated pile cutting machine would be able to bring improvements in safety, productivity, quality as well as cost saving.

      • 복숭아 유리나방의 성 페로몬의 한 성분인 (Z,Z)-3,13-옥타데카디엔-1-일 아세테이트의 합성

        姜錫久,文炳昊 성균관대학교 기초과학연구소 1987 論文集 Vol.38 No.2

        A synthesis of (Z, Z)-3, 13-octadecadien-1-yl acetate, one component of the sex pheromone of Synanthedon hector, Butler is described. Starting from 1,9-nonanediol, a sterocontrolled synthesis of (Z, Z)-3, 13-octadecadien-1-yl acetate with high sterochemical purity was achieved by the use of Wittig-olefination reaction and catalytic hydrogenation for the introduction of (Z)-double bond.

      • KCI등재후보

        심상성 루프스에서 Nested-Primer Gene Amplification Assay에 의한 결핵균 DNA의 검출

        신문석,전효진,김병천,이규석,김재룡,권호준,송준영 啓明大學校 醫科大學 1994 계명의대학술지 Vol.13 No.4

        Lupus vulgaris, which is a progressive form of postprimary tuberculosis in patients with a moderate to high degree of immunity and tuberculin sensitivity, is the most common, most serious, and most variable type of cutaneous and mucous membrane tuberculosis. Domonstration of M.tuberculosis directly or in culture in some of these eruptions can be difficult. It is essential to demonstrate the presence of M.tuberculosis in a clinical sample for a definitive diagnosis of tuberculosis. With the discovery of the polymerase chain reaction (PCR), the DNA diagnostic approach for infection has developed in a short period. With the dual purpose of definitive and rapid diagnosis of tuberculosis, this study examined the usefulness of a nested PCR for detection of M. turberculosis. Compared with a single-step PCR, the two-step PCR was able to enhance sensitivity approximately 1,000-fold on the basis of bacterial counts and DNA quantity. We concluded that two-step nested PCR appeared to be the most useful PCR protocol for early and sensitive diagnostic method detecting M.tuberculosis in acid-fast stain negative specimens.

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