Reduction of Bacillus cereus Contamination in Biofilms on Stainless Steel Surfaces by Application of Sanitizers and Commercial Detergent

Lee, Min-Jeong,Ha, Ji-Hyoung,Kim, Yong-Su,Ryu, Jee-Hoon,Ha, Sang-Do The Korean Society for Applied Biological Chemistr 2010 Applied Biological Chemistry (Appl Biol Chem) Vol.53 No.1

We established the effectiveness of a washing, detergent and sanitizer treatments for reducing Bacillus cereus (B. cereus) contamination in biofilms on stainless steel surfaces. A water treatment did not affect biofilms and chlorine treatments at 200 ppm for 10 min and 300 ppm for 5 min, iodophor at 200 ppm for 10 min, and QAC at 400 ppm for 10 min were used to eliminate all B. cereus cells. B. cereus cells after detergent treatment were eliminated by chlorine at 100 ppm for 10 min, 200 ppm for 5 min, and 300 ppm for 3 min, and with iodophor at 100 ppm for 10 min and 200 ppm for 10 min, and QAC at 400 ppm for 10 min.

이동형 스크러버를 이용한 암모니아 및 톨루엔의 제거 효율

김재영 ( Jae-young Kim ),김장윤 ( Jang-yoon Kim ),이연희 ( Yeon Hee Lee ),김민선 ( Min Sun Kim ),김민수 ( Min-su Kim ),김현지 ( Hyun Ji Kim ),류태인 ( Tae In Ryu ),정재형 ( Jae Hyeong Jeong ),황승율 ( Seung-ryul Hwang ),김균 ( Ky 한국환경농학회 2018 한국환경농학회지 Vol.37 No.1

본 연구는 국내에서 다량 취급되고 있는 암모니아 및 톨루엔을 흄 상태로 노출시킨 후 흡수/흡착방법을 달리한 이동형 스크러버를 이용해 각 유해화학물질의 제거효율을 비교 분석하였다. 이동형 스크러버는 기 개발된 장치를 개선하여 와류세정에 의한 흡수, 유입풍속 조절의 장점을 살리고, 활성탄 및 카본필터를 통한 기체상 유해화학물질의 흡착방법 도입을 통해 단점을 보완하였다. 개선된 장치는 기초성능평가를 통해 적정 제어풍속이 검증되었고, 5% 암모니아수 용액을 흄 상태로 노출시켜 후드 흡입부, 송풍기 배출부 및 세정기가 정상 작동함을 확인하였다. 흡수/흡착방법에 따른 암모니아 제거효율은 90분 경과 후 C≥PCA>SWA 순으로 가장 우수한 SWA 군의 노출 농도별 제거 효율은 시간이 경과할수록 노출 농도와 무관하게 배출 농도는 증가되었고, 세정액의 pH는 산성에서 염기성으로 변화되었다. 또한, 시간 경과에 따른 노출 농도 별 세정액의 pH 변화와 배출구 농도 변화 사이에 0.9429~0.9491 수준의 정의 상관관계를 나타내었다. 흡착방법에 따른 톨루엔의 제거 효율은 초기 10분 경과 후 배출구농도에서 C>CMA≥GCA 순을 나타내다 90분 경과까지 일정한 농도로 유지되었다. 가장 우수한 GCA 군의 노출 농도 별 제거 효율은 초기 10분 경과 시 노출 농도에 비례하여 배출 농도가 높은 경향이었다. 이와 같은 결과를 통해 개선된 이동형 스크러버는 중화반응에 의해 산성 또는 염기성 유해화학물질의 제거뿐 아니라, 활성탄 등의 흡착기능 개선을 통해 VOCs 제거에도 효과적이었다. 하지만, 실제 현장에서 활용이 가능하도록 세정액 pH를 지속적으로 유지하여 연속적으로 흡수 제거할 수 있는 방법과 노출되는 화학물질 농도와 흡착제의 관계에서 파괴점, 포화점, 흡착속도 등의 물리적인 요소가 추가적인 연구를 통해 도출되어야 할 것이다. BACKGROUND: The mobile vortex wet scrubber was developed to remove the harmful chemicals from accidental releases. However, there was a disadvantage that it was limitedly used for volatile organic compounds (VOCs) such as toluene according to the physicochemical properties. This study compared the removal efficiencies of an improved mobile scrubber on toluene and ammonia by applying diverse adsorption and absorption methods. METHODS AND RESULTS: The removal efficiencies on harmful chemicals were examined using various adsorption and absorption methods of water vortex process (C), phosphoric acid-impregnated activated carbon adsorption (PCA), pH-controlled water (pH 2.5) vortex process absorption with sulfuric acid (SWA) after ammonia exposure, granular activated carbon adsorption (GCA), and activated carbon mat adsorption (CMA) after toluene exposure. As a result, the best removal efficiency was shown in the SWA for ammonia and GCA for toluene. Also, the SWA and GCA methods were compared with different concentration levels. In the case of ammonia exposure (5, 10 and 25%), there was no difference by concentration levels, and the concentration in the outlet gradually increased, with pH change from acid to base. In the case of toluene exposure (50, 75 and 100%), the outlet concentration was higher relative to the exposure concentration in the initial 10 min, but the outlet concentration was remained steady after 10 min. CONCLUSION: The newly improved mobile scrubber was also effective in removing VOCs through adsorption techniques (activated carbon, activated carbon fiber, carbon mat filter etc.), as well as removing acid-base harmful chemicals by neutralization reaction.

유방암 환자에서 bcl-2 단백 발현의 예후 인자로서의 의의

류진우,박종권,정민,박동국,김정택,이두선,이찬영,전호진 단국대학교 1998 論文集 Vol.32 No.-

Breast cancer in women pose a problem, and it can be difficult at times to evaluate the possibility of the cancer recurrence in node negative early cancer. The reliable prognostic markers are needed in breast cancer management to help clinicians identify the patient with the high risk of recurrence and arrive at more rational decisions for proper treatment. In order to investigate the prognostic significance of bcl-2, an immunohis-tochemical staining was performed in 35 tissue sections from paraffin blocks of primary breast cancer. Bcl-2 positivity was 43%. No relation could be observed between bcl-2 expression and known prognostic factors(tumor size, lymph node metastasis state, histologic grade, and nuclear grade), There was strong relation between bcl-2 expression and estrogen receptor positivity (ER) (P〈0.05). No relation is found between bcl-2 expression and c - erbB - 2 expression. These results suggest that bcl-2 expression is associated with a favorable prognostic factor(ER) but it is of no value as independent prognostic factor to the clinician in identifying the patients with high risk of recurrence.

대구지역에서 꿀벌이 수집한 화분의 동정

류장발,장정원,김기만,권외정,김민식,박도규 대구대학교 생명과학연구소 2002 생명과학연구 Vol.1 No.2

Pollens gathered by bees at an apiary at Daegu district were identified with light microscope and scanning electro microscope for effective establishment of honey plant plantations for year-round honey production. Pollen loads were collected from bees from March to October at one week interval, ten loads at each collecting time, a load from a colony. Pollens from fifty-four species were collected and 34 species were identified and 20 species unidentified, which would be identified later. Most of the pollen loads were composed with one species. However, a few loads were composed with up to four species. Pollens of 37 species were collected just one time. Pollens from just one species were not collected at any time. In July pollens from many species, up to six species at July 15 and 20, were collected. Pollens of four species, Erigeron annuus, Lagerstroemia indica, Koelreuteria paniculata, Evodia daniellii, were collected up to one month. As the latter two species were known as good honey producing plants, these two species proved to be good pollen producing plants. The former two species were known as poor honey producing plants, the importance of these two species should be reevaluated.

체육수업 설계에 대한 고등학생의 기대요구 분석

이정흔,오병돈,조민행,유영설,이명선,조병준 韓國學校保健學會 2009 韓國學校保健學會誌 Vol.22 No.2

Purpose: The purpose of this study was to analyze students' expectance demand towards high school physical education teaching. Methords : 392 participants randomly selected from one girls' middle school and one girl's high school in each of five tracts and then selected two classes from each of the schools. They were asked to respond to a simple Questionnaire about their views on the present physical education teaching. Results : Results showed that about one third of the participants would not choose physical education if the program were offered as an optional subject. Both male and female students ranked psychomotor domain as the top priority among the various learning objectives. Male students favored soccer and basketball while their counterparts liked badminton and volleyball. Over three-fourths of the participants suggested that students' opinions should be considered when teachers plan their program. Conclusion : The results of this study recommended that physical education teaching should be designed with considerations for professional values, societal changes as well as students' expectance on the design of physical education.

Biocompatible Osteoconductive Polymer와 吸收性 Hydroxyapatite가 骨化에 미치는 影響에 關한 硏究 : A HISTOLOGICAL STUDY

李敏汀,李相喆,柳東睦 慶熙大學校 齒科大學 1995 慶熙齒大論文集 Vol.17 No.2

The purpose of this study was to observe the osteogenic effects of biocompatible osteoconductive polymer(BOP D.T.I. Co., U.S.A.) and resorbable HA(Osteogen ; Impladent Co., U.S.A.) in bony defected area In order to perform this study, 30 male rabbits, weighing about 2.5kg were selected. The 3x5x7mm sized defects were made on the both sides of the mandibular body and the defect was filled with blood clot in control group and with resorbable HA and BOP respectively in experimental groups. All experimental animals were sacrificed at the 3rd day and the 1st, 2nd, 4th and 6th week after operation. The mandibular body and surrounding tissues were removed out, deminerahzed, dehydrated and embedded. The histological observation of the specimens in light microscopic level was performed after Azan staining. The results were as follows 1. Osteoblasts were observed after a eek both in control group and experimental groups, and fibrous tissues were formed in bony defected area. Especially, new bone formation was observed earlier in BOP experimental group than in any other groups. 2. New bone formation was developed from the adjacent normal bone to bony defected area both in control group and experimental groups. And in experimental groups, new bone was infiltrated rapidly into the graft material than in control group and large amount of bone formation was observed. 3. Both in two experimental groups, the resorption pattern of the graft material was observed after 2 weeks, an materials were almost disappeared after 4 weeks. 4. After 6 weeks, bony defected area was occupied with new bone both in control group and in experimental groups, but bony density in experimental groups was higher than in control group, especially in BOP experimental group which showed more mature heating state.

치은부에 이중인공진피(Terudermis^(�) 이식 후 조직학적 평가

김민정,정현주,류승희,박병주 전남대학교 치의학연구소 2003 구강과학 Vol.15 No.4

The autogenous free gingival graft is the most predictable procedure currently used to increase the width of the attached gingiva in periodontal therapy. But the major disadvantage of the procedure is to create the 2ndary multiple surgical wounds at both a donor site and a recipient site. The other problem is the limited amount of available graft material. Therefore, recent researches have been focused on the development of the biomaterial to substitute gingival tissue. The purpose of this study was to compare the histologic healing after grafting of bilayer artificial dermis and free gingival graft. Four non-smoking subjects (mean age, 32.5 years) in systemically healthy state and good oral hygiene were selected according to their particular needs for correction of mucogingival deformities as suggested by Nabers : 1) zones of minimal attached gingiva, 2) areas of thin gingiva, 3) areas of inadequate vestibular formation, and 4) frenum problems. The recipient sites were prepared through the conventional free gingival graft procedure and were grouped according to the graft materials : Experimental group-bilayer artificial dermis (Terudermis ; Terumo Co. Japan)(n=5) ; Control group-free gingival graft with autogenous palatal mucosa(n=6). Biopsies were harvested from 11 sites (containing adjacent attached gingiva and graft) at 1, 2, 3 and 6 weeks postsurgery to evaluate histologic healing. At the third week in the experimental group and at the second week of in the control group, the grafts has been stabilized on the recipient bed and the graft border has been blended into the surrounding tissue. In the experimental group, the epitheial migration from the adjacent tissue to graft was observed after 1 week of grafting and the distribution of the inflammatory cells were reduced, collagen layer of the artificial dermis was lost and the basement membrane was formed after 3 weeks of grafting. At 6 weeks of grafting, both groups demonstrated orthokeratinized epithelium and increased thickness of epithelial tissue, similar to the adjacent tissue and the rete peg formation. It was difficult to distinguish the graft from adjacent normal tissue. Histologic evaluation revealed a biologic acceptance and incorporation of the collagen layers of the graft tissue to the host tissue, without severe inflammatory response. In conclusion, a bilayer artificial dermis is essentially similar to autogeneous free gingival graft in the correction of mucogingival problems, and has the advantages of decreased patient morbidity(no donor site) and availability of abundant amounts of graft material when needed.

Pentadecafluorroctyl Polyacrylate에 依한 Nylon 6 纖維의 撥水加工에 關한 硏究

李廷玟,任洪彬,劉承坤,裵基瑞 충남대학교 공업교육연구소 1978 論文集 Vol.1 No.2

This study has been investigated on the effects of fluorochemical repellent agent on the water repellent finishes of nylon 6 fibre with pentadecafluorooctyl polyacrylate. Contact angles of various liquids on the fluorochemical treated nylon 6 surface are measured. The critical surface tension of about 46 dynes per cm on the nylon 6 surface is resulted. The effect of fluorochemical repellent was most favourable at 1% concentration repellent agent and the critical surface tension is resulted the value of about 10.5 dynes per cm. The use of antistatic agent in the fluorochemical repellent was most favourable below 0.5% concentration. It was found that the repellent effects were improved by repeated treatments with the solution of lower concentration of repellent.

優秀工業系 實科敎員養成을 위한 工業敎育大學 敎育課程 改善에 關한 硏究 : 工業敎育學部를 中心으로

李廷玟,任洪彬,李載元,李來然,金澤基,劉承坤 충남대학교 공업교육연구소 1983 論文集 Vol.6 No.2(A)

This paper is studied to improve the curriculum of the College of Industrial Education of Chungnam National University, which is more helpful for the students to become a competent teacher of technical high school. The thesis includes the curriculum of general, professional, technical subjects and so on. The main conclusions are summarized as follows; 1) The current curriculum of the College of Industrial Education was formed on a wholesale manner following the basis of standard courses for some characterized engineering colleges. But the suggested is conformed to the curriculum system of CNU in credits and hours. 2) The current technical subjects are mainly based on the curriculum of college of engineering, but the newly suggested technical subjects are conformed to the curriculum of technical high school as following that some departments of engineering are founded in the College of Industrial Education. 3) In current curriculum, the ratio of reguired technical subjects to elective technical subjects is 85:15. In suggested curriculum, the ratio of it is largely changed to 45:55 for effective operation of minor cousres. And the lists of the subjects for minor courses are changed from subjects for College of Science to subjects for College of Engineering.

한국구강보건사업이 초등학교 아동들의 유치 및 영구치 우식실태에 미치는 영향

최순례,유영아,조민정,송근배 韓國學校保健學會 2004 韓國學校保健學會誌 Vol.17 No.2

Purpose : The aim of this study was to evaluate the effects of oral health care programs in a school-based oral health care center among primary schoolchildren. Methods : School-based oral health care programs included fluoride mouth rinsing, pit and fissure sealing for permanent premolars and molars, fluoride gel application and chewing of xylitol candy. All of the programs were carried out by one dental hygienist among 'D' primary schoolchildren in Daegu city under the supervision of a dentist. Baseline dental examinations were completed and preventive care was implemented for 544 children during one year. All of the children visited a school-based oral health care center every three months for a regular check-up. The final oral examination was conducted from March 15 to April 1, 2004. The data analysis data was made on the basis of SAS 8.01. Mean differences between 2003 and 2004 data were compared by paired t-test. Corresponding p-values were considered significant at values less than 0.05. Results : The DMF rate and DFT index were reduced to 8.0% and 8.4% during one year respectively, but there were no statistically significant differences. The DMF rate was significantly reduced (16.3%) after a one year program of school-based oral health care practice. The DMFT(Decay Missing Filling Tooth) index was also reduced compared to 2003 throughout the entire grade. Conclusion : School-based oral health care programs can reduce the prevalence of dental caries prevalence among schoolchildren during one year. This program also improved the oral health capacity of schoolchildren. It is recommend that the school-based oral health care program should be extended to every primary school in Korea