Yeast로 부터 RNA 抽出에 關한 硏究

劉承坤,任洪彬,周赫鍾 충남대학교 1977 工業技術開發硏究所論文集 Vol.4 No.1

In the extraction of RNA from Sccharomyces cerevisiae, sodium chloride solution is regarded as the proper solvent and 5 percent is selected as the optimal concentration. The optimal temperature and time in the extraction of RNA are obtained at 65℃ for 60 minutes. The amount of solvent per unit gram of yeast is scarcely affected at more than 200ml. Yeast powder which was pretreated with hot TCA products about 10 times of RNA than yeast cake. In the case of yeast cake, the amount of RNA extracted was suddenly increased over the sterilizing temperature of yeast. Although yeast was pretreated with hot TCA, the amount of RNA extracted is only 66% of total amount of RNA by the first stage. But 93% of RNA is extracted by the continuous two stages.

梔子로부터 梔子油 抽出에 關한 硏究

劉承坤 충남대학교 공업기술개발연구소 1976 工業技術開發硏究所論文集 Vol.3 No.1

Gardenia contains about 20wt% of Gardenia oil. Ethyl-Ether was selected as solvent for extraction of Gardenia oil from Gardenia particles that was passed 18 mesh Tailer style screen. The amounts of Gardenia oil extracted from unit Gardenia sample was propotional to the log θ^2.5 fo extaction time (θ; 10∼240min.), the log T^15 of extraction temperature (T; 10∼30℃), and the log V^5.2 of volume of solvent (V; 10∼200ml/5g sample). The optimum extraction conditions were 120 min., 20℃ and 10ml of solvent per gr Gardenia particles. Finally, the empirical equation was derived as fallow, Y(θ.T.V)??log θ^2.5T^15V^5.2―20.5 The devition the experimental value and the one calculated from the equation in effective range is ±0.5%. The results of analysis of Gardenia oil were those that the sapoification value was 220∼240, the acid value was 65∼72, the iodine value was 46∼52 and the specific gravity was 0.925∼0.945 (25℃/25℃).

활성탄소섬유 카트리지 휠터에 의한 수중 잔류염소의 흡 탈착 거동

유승곤,이재광,송승필 한국화학공학회 2000 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.38 No.2

활성탄소섬유 카트리지 휠터를 제조하고 이 휠터의 수중 잔류염소 흡착 및 탈착 거동을 조사하였다. 2 ppm 잔류염소의 흡착파과곡선에서 0.2 ppm의 잔류염소농도를 파과점으로 할 때, 파과점까지 활성탄소섬유 카트리지 휠터(Activated Carbon Fiber 함량: 100 g)는 31.5 ton, 활성탄소섬유/활성탄 카트리지 휠터(Activated Carbon Fiber 함량: 50 g, Activated Carbon 함량: 50g)는 8.4 ton을 정수하였다. 카트리지 휠터에 의한 압력손실은 유량이 10ℓ/min일 경우, 활성탄소섬유 휠터의 경우는 120 ton 처리까지 0.2 ㎏_f/㎠ 미만이나, 활성탄소섬유와 활성탄을 1 : 1로 혼합하여 만든 경우는 120 ton처리까지 2.3㎏_f/㎠ 이었다. 카트리지 휠터의 탈착은 90℃의 뜨거운 물로 역세척하여 수행하였다 충분히 역세척이 이루어진 카트리지 휠터의 파과점까지의 제2차 수중 잔류염소 흡착실험으로 탈착률(재생률)을 검사하였다. 그 결과 활성탄소섬유로만 사용한 카트리지 휠터의 탈착률은 70.5%이었으나, 활성탄소섬유와 활성탄을 1 : 1로 혼합하여 만든 카트리지 휠터의 탈착률은 36.9%이었다. 활성탄소섬유 카트리지 휠터의 탈착률은 역세척수의 온도가 상승할수록 상승했으며, 활성탄이 더해짐에 따라 잔류염소 탈착률이 감소했다. Cylindrical Activated Carbon Fiber cartridge filters were prepared to investigate their adsorption/desorption behavior of residual chlorine from 2 ppm aqueous solution. From breakthrough profiles, the amount of purified water was 31.5 ton with Activated Carbon Fiber cartridge filter(Activated Carbon Fiber: 100g) while only 8.4 ton with Activated Carbon Fiber/Activated Carbon cartridge filter(Activated Carbon Fiber; 50 g, Activated Carbon: 50 g) at the breakpoint when the breakpoint was fixed to 0.2 ppm(C/C_0=0.1) residual chlorine. The pressure drop through Activated Carbon Fiber and Activated Carbon Fiber/Activated Carbon cartridge filter were 0.2 ㎏_f/㎠ and 2.3 ㎏_f/㎠, respectively, when 120 ton aqueous solution was passing the cartridges at 10ℓ/min flow rate. The desorption of chlorine from fully used cartridges was performed by reverse washing with hot water at 90 ℃. The desorption efficiency of cartridge was determined by comparison the 2nd adsorption capacity of residual chlorine with the 1st adsorption capacity at breakpoint. The desorption efficiency of Activated Carbon Fiber cartridge was 70.5% while that of Activated Carbon Fiber/Activated Carbon cartridge was 36.9%. The desorption efficiency of cartridge increased with the temperature of washing water and decreased with the amount of Activated Carbon charged in cartridge.

生物體로부터 天然化合物 抽出에 관한 硏究 : 第 III 報 밤송이 껍질로부터 赤褐色 色素 抽出에 關한 硏究 Part 3. Extraction of Reddish-Brown Pigment from Chestnut-bur

劉承坤 연세대학교 대학원 1974 원우론집 Vol.2 No.1

Abstract The extraction efficiency of reddish-brown pigment from the chestnut-bur was greatly depended upon the extraction time, temperature and the amounts of solvent used. From the experimental results, the amounts of extracted pigment was logarithmically propertional to the extraction time and the amounts of solvent used for the extraction and also linearly proportional to the temperature of extraction, as the following equation which was empirically determined. Ptotal = 0.32logθ+0.485logS+0.022T-0.34 In addition to these, it wart very efficient that the sample was dried at 110℃ for 20minutes, prior to the pigment extraction. Finally, the most optimum conditions of pigment extraction were determined as 60 minutes of operation time, 60℃ of temperature and 400ml of solvent, 20% elhyl-alcohol per 1 g of the sample in the extractions.

96 춘계총회 및 학술발표회를 마치고

유승곤 한국화학공학회 1996 NICE Vol.14 No.4

향류식 열교환기에 의하여 멸균된 된장의 미생물군 및 색도

유승곤,김인호,김종생,최성현,오만진,김용국,이인기 한국생물공학회 1998 KSBB Journal Vol.13 No.6

To develop a large scale countercurrent single pass heat exchanger for continuous transportation and sterilization of soybean paste, microflora and color value of sterilized soybean paste were examined at various sterile condition. Aerobes, anaerobes, molds, yeasts and lactic acid bacteria were 5.1 x 107 CFU/g, 7.1 x 107 CFU/g, 2.6 x 105 CFU/g, 4.3 x 106 CFU/g, 1.3 x 107 CFU/g, respectively in raw soybean paste. In gold band ampoule test, aerobes and anaerobes of soybean paste were viable up to 90$^{\circ}C$, but become unviable at 100$^{\circ}C$. Molds decreased rapidly and yeasts decreased slowly from 70$^{\circ}C$. Lactic acid bacteria were unviable at 60$^{\circ}C$ within 10 min. In color test, Hunter L, a, and b values of soybean paste were 50.2, +5.6, and +17.8, respectively. After heating in polyethylene film bag at 80$^{\circ}C$, Hunter values were not so much changed and become 50.2, +4.7, and +19.7, respectively. The micorflora and color of soybean paste sterilized in a large scale heat exchanger system resulted in very similar to those of gold band ampoule and polyethylene film bag by effective heat transfer.

활성탄소섬유의 수중 페놀의 흡착

이재광,심재운,송승필,유승곤 忠南大學校 産業技術硏究所 1998 산업기술연구논문집 Vol.13 No.1

Adsorption behavior of activated carbon fiber cartridge for phenol of drinking water was studied. Adsorption equilibrium data were obtained from batch experiments. The ACF used for cartridge shows 1600㎡/g specific surface area, 7.7Å average pore radius, 0.52㎤/g micro-pore volume, and 0.16g/g-ACF adsorption amount of phenol. In removal of phenol from tap water by ACF cartridge, the breakthrough curves were rapidly increased from C/C。=0.1 to C/C。=0.5 and slowly approached C/C。=1.0. One ACF cartridge was made of l00g activated carbon fiber and a total amount of teated water from 2.0ppm to 0.2ppm(break-point C/C。=0.1) by one ACF cartridge was 751ℓ. The adsorption efficiency of ACF cartridge was 45% of its maximum theoretical adsorption capacity.

연구발표논문초록 : 전달현상 ( 2 ) ; 탄소섬유 - 글리세린의 써스펜젼계인 비뉴톤유체가 Converging Channel 을 통과할때 유동학적 특성연구

유승곤,김태진,최정봉 한국화학공학회 1985 추계총회초록집 Vol.- No.-

植物體의 水溶性 成分의 抽出 메카니즘

유승곤,송봉준,이보성 충남대학교 공업교육연구소 1985 論文集 Vol.8 No.1

Water soluble components of Persicaria hydropiper have an insecticidal effect. The extraction mechanism of water solubles from Persicaria hydropiper was studied and it turned out to be a diffusion model. The diffusivity was logarithmically increased with respect to extraction temperature (20…70℃) showing the values in 0.7…2.65×10^-7 cm²/min. Some variables which have an effect on extracted quantity of water solubles were also observed. As a result, optimum operatin conditions and empirical equation were presented for the industrial scale extraction.

부가결정화를 이용하여 납사분해유의 C9 방향족 혼합물로부터 Pseudocumene 분리

유승곤,이철호,김광주 한국화학공학회 1992 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.30 No.6

부가결정화 기술을 이용하여 납사분해센타에서 부생되는 C_9 방향족 혼합물로부터 pseudocumene을 분리하였다. 원료투입비, 결정화온도, 용매비, 냉각속도 등을 변수로 하여 thiourea 부가특성을 조사하였으며, pseudocumene을 분리할 수 있는 최적조건을 조사하였다. Pseudocumene은 C_9 방향족 혼합물로부터 4단계 부가결정화 조작에 의해서 99.7 wt%의 순도로 분리될 수 있었다. Trimethylbenzene 이성체에 대하여 thiourea에 부가되는 경향은 pseudocumene>hemimellitene>>mesitylene의 순이었다. C_9 방향족 화합물의 이성분계에 대한 부가결정화의 분리도를 추출결정화 및 증류의 분리도와 비교하였다. 이들 이성분계에 대하여 부가결정화에 의한 분리정도(extent of separation)는 한 이론 증류단수의 최대분리정도 보다 5.4-24배 컸으며, 추출결정화에 비해 1.9배 컸다. 또한 부가결정화의 한 단(stage)에서 분리할 수 있는 thiourea의 포획도는 추출결정화에 비해 6배정도 컸다. The separation of pseudocumene from the C_9 aromatic raffinate found in naphtha cracker was studied using adductive crystallization with thiourea. The characteristics of thiourea adduction are investigated on the variables of feed ratio, temperature, solvent ratio and cooling rate, and optimum conditions on the separation of pseudocumene are investigated on these variables. Pseudocumene can be separated by four stages adductive crystallization from C_9 aromatics as a purity of 99.7 wt%. With the trimethylbenzene isomers, the tendency for thiourea adduction is pseudocumene>hemimellitene>>mesitylene. The separation capacities of adductive crystallization for two binary mixtures of C_9 aromatics were compared with those of distillation and extractive crystallization. The extent of separation by one adduction is approximately 5.4 to 24.0 times greater than maximum separation that could be obtained by one theoretical distillation stage. The entrapping capacity is approximately 6.0 times greater than the entrapping capacity that could be obtained by extractive crystallization.