영지(Ganoderma lucidum)균사체의 액체배양에 의한 세포외 수용성 다당류의 분획 및 항암활성

이신영,강태수,문순옥,류인덕,이명열 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

영지 균사체의 액체배양에 의하여 생산된 세포외 다당 시료를 분획하였고, 이들 각 분획에 대한 항암 활성을 균사체 유래 다당 분획들과 비교하면서 조사하였다. 배양여액으로부터 세포외 수용성 다당(BWS)을 얻었고, 이를 순차적으로 이온교환 및 겔 크로마토그라피 하여 BWS를 포함한 세포의 수용성 다당 분획, 총 5종을 얻었으며, 균사체 유래의 수용성 다당(MWS)에서도 세포외 다당과 동일한 방법으로 분획하여 총 5종의 분획을 얻어 대조구로 시용하였다. 세포외 다당 시료들의 항보체 활성(ITCH_50, %)은 15~30% 정도로 15~70%인 균사체 유래 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들의 값보다는 낮았으나, 이들 세포외 다당 분획들중, BWS-DA와 BWS-DA-GI는 1000 ㎍/ml의 시료 농도에서 30% 정도의 매우 높은 활성을 나타내었다. MTT assay에서는 mouse leukemia L1210에 대하여 BWS-DN 및 MWS가 600㎍/ml의 농도에서 각각 86 및 89%의 높은 저해율을 나타내었으며, BWS-DA 및 MWS-DN의 경우는 3㎍/ml의 매우 낮은 농도에서도 32~35%의 저해율을 보였다. 또 human cell line인 COLO-205에 대해서는 BWS와 BWS-DA 및 MWS-DA가, 그리고 HL-60에 대해서는 BWS-DA와 BWS-DN 및 MWS-DN이 각각 300㎍/ml의 시료 농도에서 약 50%(IC_50)의 높은 저해능을 나타내었다. Exo-polysaccharide (BWS) obtained from submerged cultivation of Ganoderma lucidum mycelium was fractionated. Antitumor activity of their fractions was investigated in comparison with the mycelial polysaccharide fractions. Eight kinds (BWS-DN, BWS-DA, BWS-DN-GI, BWS-DA-GI, MWS-DN, MWS-DA, MWS-DN-GI and MWS-DA-GI) of polysaccharide fractions were obtained by DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel chromatography from BWS and MWS, which were isolated from culture fluid and mycelial cell, respectively. The anticomplementary activities (ITCH_50, %) of the exo-polysaccharide fractions showing 15% to 30% were lower than those of mycelial polysaccharide fractions showing 15% to 70%. The acidic fractions of BWS-DA and BWS-DA-GI fractionated from BWS, showed the highest activity of 30%. In the MTT assay, BWS-DN and MWS against mouse leukenia L1210 exhibited high inhibition ratio of 86 and 89%, respectively at the concentration of 600 ㎍/ml. High inhibition ratio of 50% (IC_50) was achieved for BWS, BWS-DA and MWS-DA fractions against human colon adenocarcinoma COLO-205 and for BWS-DN, and MWS-DN fractions against human leukemia HL-60 at the concentration of 300 ㎍/ml among the six polysaccharide fractions, respectively.

Ultra-energy-efficient analog-to-digital converters based on single-electron transistor/CMOS hybrid technology for biomedical applications

Lee, Jieun,Choi, Changmin,Park, Sungwook,Chung, In-Young,Kim, Chang-Joon,Park, Byung-Gook,Kim, Dong Myong,Kim, Dae Hwan Institute of Physics 2009 Semiconductor science and technology Vol.24 No.11

<P>Ultra-energy-efficient analog-to-digital converters (ADCs) based on single-electron transistor (SET)/complementary metal-oxide-semiconductor (CMOS) hybrid technology are proposed as a solution to sense and process biomedical signals. Our results show the energy efficiency of 0.82 pJ/state, which is lower than that in previously reported energy-efficient ADCs. The performance and dissipated power of proposed ADCs are estimated and compared with those of CMOS ADCs by using Lee's SPICE model including non-ideal effects of the experimental data. While the proposed ADC shows an operating power lower by two orders of magnitude than that of the CMOS flash-type ADC, the number of required transistors is about 10% of that in the CMOS flash-type ADC. The peak-to-valley current ratio in Coulomb oscillation of SETs used in the circuit implementation has the range of 1.15–1.5, which is consistent with the experimental result of top-down approached Si-based SETs at <I>T</I> = 77–100 K. From the perspective of the immunity to the gate capacitance <I>C<SUB>cg</SUB></I> mismatch and the background charge <I>Q</I><SUB>0</SUB> noise, it is shown that the criteria of SET/CMOS hybrid ADCs are Δ<I>C<SUB>cg</SUB></I> ⩽ 0.02 × <I>C<SUB>cg</SUB></I> (with <I>C<SUB>cg</SUB></I> = 0.24 aF) and Δ<I>Q</I><SUB>0</SUB> ⩽ 0.23<I>q</I>, respectively.</P>

치과용 아말감의 산화환원에 관한 전기화학적 연구

이인복,이명종 大韓齒科保存學會 1993 Restorative Dentistry & Endodontics Vol.18 No.2

The purpose of this study was to observe corrosion characteristics of six dental amalgams and was to analyse corrosion products electrochemically. After each amalgam alloy and Hg was triturated as the direction of the manufacturer by using mechanical amalgamator, the triturated mass was inserted into the cylinderical metal mold(12X10mm) and was condensed with 160kg/cm^2 by using the hydrolic press. The specimen was removed from the mold and was stored at room temperature for 1 week, and was polished with amalgam polishing kit. The anodic and cathodic polarization curve was obtained by using cyclic voltammetric method with 3-electrode potentiostat range was -1.7V~0.4V(vs SCE) in working eledtrode and scan rate was 50mV/s and the exposed surface area of each specimen to the electrolytic solution was 0.79cm^2. The results were as follows. 1. In anodic-cathodic polarization curve of amalgam specimens, two anodic current rising areas and two cathodic current peaks were obtained at the low Cu amalgam(CF, CS) specimen and three anodic current rising areas and three cathodic current peaks were obtained at the high Cu amalgam(TY, DS, HV) specimen. 2.As this compared with the anodic and cathodic current peak potentials of Sn, Cu and Ag specimen, the first cathodic current peak I c was caused by the reduction of divalent tin salt, second cathodic current peak II c results from the reduction of quadravalent tin salt, and third cathodic current peak III c results from the reduction of copper salt. 3. As reverse potential sweeping was done repeatedly, anodic current was decreased slightly in all amalgam specimens.

最適序列 算法을 利用한 電力系統 分割 解法의 開發

조인숙,이춘모,신명철 成均館大學校 科學技術硏究所 1990 論文集 Vol.41 No.1

Today, the power systems have been complicated and large-scaled. In order to analyze such system promptly and maintain it efficiently, the application of computer to power system is introduced. Accordingly, the interest in analysis of power system by computer has been growing around the world. But there are many additional problems, such as inaccurate solution, computing time delay, and immense memory size. This paper deals with ordering algorithm and diakoptic method in large eletric power system, and its main concern is significant reduction in computing time and memory size. In this thesis a new diakoptic method is designed. It takes the good points of diakoptic method and MPRLD, a kind of optimal ordering algorithm, which had improved sparse vector method. Reducing in computing time and memory size by the diakoptic method designed is verified with a model system. It is possible to reduce because each teared area is analyzed independently and the concept of parallel processing is adopted.

Effects of In-Situ Annealed Buffer Layers in Oxygen Plasma on Properties of ZnO Epitaxial Layers Grown by Plasma-Assisted Molecular Beam Epitaxy

Min Su Kim,Do Yeob Kim,Ghun Sik Kim,Hyun Young Choi,Min Young Cho,Su Min Jeon,Kwang Gug Yim,Ji Hoon Kim,Dong-Yul Lee,Joo In Lee,Sung Dong Park,Eundo Kim,Myong Hyo Jung,Do-Weon Hwang,Jae-Young Leem 한국진공학회 2009 한국진공학회 학술발표회초록집 Vol.37 No.-

'1998 한국인 우울증 선별의 날' 참가자들의 사회인구통계학적 특성과 우울증 선별검사 결과

한상익,이성필,고효진,박원명,장계호,유태열,전태연,채정호,방승규,김광수,최보문,이정태,백인호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.5

연구목적 : 1998년 정신건강의 날 기념 한국인 우울증 선별의 날 행사에 자발적으로 참여한 참가자들의 사회인구 통계학적 특성을 분석하고 우울증 선별검사 결과를 평가하기 위하여 본 연구를 시행하였다. 방 법 : 8개 병원에서 얻어진 619명의 자료를 사용하여 선별검사로 밝혀진 우울증의 유병율과 사회인구통계학적 특성 및 응답자의 정신과적 치료를 포함한 과거력 등을 조사하였다. 결 과 : 우울증 척도 점수는 응답자 전체 평균 57.5±13.8로 경도의 우울 증상이 있는 것에 해당되었다. 전체 응답자의 69.5%(430명)가 최소한 경도 이상의 우울 증상을, 43.1%(267명)가 중등도 이상의 우울 증상을, 18.4%(114명)가 고도의 우울 증상을 보였다. 고도의 우울 증상이 있는 응답자의 56.8%와 중등도의 우울 증상이 있는 응답자의 63.9%가 정신과 치료를 받은 적이 전혀 없었다. 나이가 29세 이하인 것과 전임근무 중인 직업상태는 각각 나이가 60세 이상인 것과 직업이 없는 것에 비교하여 우울증으로부터 보호하는 인자임을 시사하였다. 결 론 : '1998 한국인 우울증 선별의 날' 행사를 통하여 많은 우울증 환자들을 선별해낼 수 있었고, 이들의 우울 증상은 몇몇 사회인구통계학적 특성과 유의한 관계가 있음을 확인하였다. 우울증 선별의 날 행사를 통한 우울증에 대한 교육과 우울증 선별검사는 치료받지 못하고 있는 우울증 환자 및 기존의 치료 중인 환자 모두에게 유용한 프로그램임을 알 수 있었다. Objectives : This study was performed to analyze the sociodemographic characteristics of participants in '1998 Korean Depression Screening Day' and to evaluate the results of the screening test. Methods : By using the survey results of 619 volunteers from 8 hospitals, the authors examined the prevalence of depression detected at the screening test and sociodemographic characteristics and the psychiatric treatment history of respondents. The assessment measure was the Zung Self-Rating Depression Scale. Results : The mean depression score of all participants was 57.5±13.8 and it came under mild depression. Of all participants, 69.5%(N=430) had at least mild depressive symptoms, 43.1%(N=267) had at least moderate symptoms, and 18.4%(N=114) had severe symptoms. Never have 56.8% of respondents in the severely depressed range and 63.9% of those in the moderately depressed range had psychiatric treatment. The results suggest that the age group of 29-year-old or younger(relative to 60-year-old or older group) and full-time employment status(relative to unemployment) are protective factors of depression. Conclusions : By '1998 Korean Depression Screening Day', many depressed patients were detected and their depressive symptoms had statistically significant relationships with some sociodemographic characteristics. The results suggest that the education and screening test programs for depressive illness facilitated by Depression Screening Day are useful to the patients regardless of being under current treatment or not.

Analysis of Atmospheric Aerosol Forecast based on Data Assimilation using Satellite and Ground Observations

Ganghan Kim,Seunghee Lee,Myong-In Lee 한국기상학회 2021 한국기상학회 학술대회 논문집 Vol.2021 No.10

Northeast Asia is the unique region in which is various atmospheric aerosol events are occurred such as Yellow Dust. To understand the atmospheric air circulations over Northeast Asia, this study develops an aerosol data assimilation and forecast system using the Weather Research and Forecasting model coupled with Chemistry (WRF-Chem) and the three-dimensional variational (3DVAR) data assimilation method. The aerosol optical depth (AOD) from the Geostationary Ocean Color Imager (GOCI), Moderate Imaging Spectroradiometer (MODIS) satellite, and surface particulate matter (PM) observations has been assimilated. The study covers Northeast Asia domains, and the modeling is evaluated for the Korea-US Air Quality (KORUS-AQ) campaign period and four seasons of 2017 periods. The result shows the underestimation of aerosol concentration in most regions, in which the assimilation of satellite and in-situ data improves the mean biases and spatial distribution. Moreover, it improves the forecast skill of the surface concentration of PM10 and PM2.5. The satellite AOD-only assimilation slightly enhances the forecast skill and most of the skill improvement comes from the surface PM assimilation, showing a practically useful level of skill until 12 hours from the initial state. The marginal improvement in the PM10 forecasts by the AOD suggests the non-negligible difference between column-representing AOD and the surface PM concentration. For the 4-season simulation, they pronounced the seasonal dependence in the forecast skill, suggesting an important role of the seasonal changes in regional atmospheric circulation patterns. The spring and autumn showed higher degradation, and the winter and summer showed relatively high performance.

Risperidone 조기 중단군 및 장기 유지군의 임상적 특성 비교

김광수,박원명,전태연,배치운,김대진,백인호,이철,김정수,한상익,최보문,장계호,고효진 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.3

연구목적 : Risperidone 투여후 조기 중단한 환자들과 장기적으로 계속 risperidone을 복용하고 있는 환자의 임상적 특성을 평가하고 나아가 risperidone장기 유지와 조기 중단의 요인을 분석하고 정신병리가 약물 투여에 미치는 영향을 조사하고자 하였다. 방 법 : 연구 대상자는 1996년 1월부터 1996년 12월까지 가톨릭대학교 의과대학 부속 8개 병원 정신과에서 risperidone을 투여 시작한 환자 580명중 DSM-IV기준에 의해 정신분열병으로 진단받고 투여 기간이 6개월 미만(조기 중단군)이거나 2년 이상(장기 유지군)을 경과하였으며 의무기록의 누락이 없는 210명을 선정하였다. 두 군간 임상적 특성을 조사하기 위하여 인구학적 변인, 정신병리학적 변인, 약물 투여기록 및 부작용 등을 과거 의무기록을 근거로 평가하였다. 결 과 : 대상환자 210명 중 조기 중단군이 67명(31.9%)이었고 장기 유지군은 143명(68.1%)이었으며 두 군간 인구학적 변인과 정신병리학적 변인에 따른 특징에는 두 군간 차이가 없었다. 두 군간 risperidone의 시작 용량과 변인과 정신병리학적 변인에 따른 특징에는 두 군간 차이가 없었다. 두 군간 risperidone의 시작 용량과 최대 용량은 유의한 차이가 없었으나 유지용량은 조기 중단군에서 4.49(±1.44)mg,장기 유지군에서 3.67(±1.50)mg으로 유의한 차이가 있었다(t=3.698, p<0.05). 또한, 이전 항정신병약물의 사용 기왕력 변인에서 조기 중단군은 기왕력이 없는 환자가 39명(58.2%), 고역가 항정신병약물을 사용한 기왕력이 있는 환자는 27명(40.3%), 저역가 항정신병약물을 사용한 기왕력이 있는 환자는 1명(1.5%)이었고, 장기 유지군은 기왕력이 없는 환자가 58명(40.6%), 고역가 항정신병약물을 사용한 기왕력이 있는 환자는 77명(53.8%), 저역가 항정신병약물을 사용한 기왕력이 있는 환자는 8명(5.6%)으로 유의한 차이가 있었다.(x²=6.559, df-=2, p<0.05). 결 론 : 본 연구결과에 의하면 가급적 환자 상태에 따라 낮은 최적 치료 용량을 투여하는 것이 장기잔의 투여를 바람직한 것으로 생각된다. 본 연구와 같은 대규모의 후향적 자연적 연구는 일반 실제 진료에서 risperidone의 효과 및 기타 다른 측면에서의 유용한 정보를 얻을 것으로 기대된다. Objective : This retrospective naturalistic study was designed to compare the clinical characteristics including psychopathology of two groups of patients, long-term maintenance group and short-term drop-out group, who were taking risperidone. Method : Datas were collected for 210 schizophrenic patients with complete medical records among 580 patients who were enrolled with risperidone administration from January 1996 to December 1996 in 8 affiliated hospital of the Catholic University. The short-term drop-out patients group were assigned to whom treatment period was less than 6 month, and the long-term maintained patients group, treatment period was more than 2 years. We assessed demographics, psychopathology, and other variables related with medication based on past medical records. Results : Among subjects of 210, short-term drop-out patients group were 67(31.9%) and long-term maintained patients group were 143(68.1%). Demographics and psychopathology were not significantly different between two groups. The stating and maximal dosage of risperidone was not significantly different between two groups but the maintenance dosage of risperidone was lower in long-term medicated patients group than short-term drop-out patients group(t=3.698, p<0.05). Additionally, the result of this study showed differences in experiences of past antipsychotic use as following. The number of no previous use of antipsychotic was 39(58.2%), the number of high potency amtipsychotic use was 27(40.3%), and the number of no previous use of antipsychotic was 58(40.6%), the number of high potency ntipsychotic use was 77(53.8%), the number of low potency antipsychotic use was8(5.6%) in long-term maintained group.(x²=6.559, df-=2, p<0.05). Conclusions : According to these results, administration of low therapeutic dosage should be recommended for long-term maintenance as if possible. Multi-center based retrospective naturalistic study like this would be useful for getting informations about efficacy and some other aspects of antipsychotic administration in practial field.

Screening with the Korean Version of the Mood Disorder Questionnaire for Bipolar Disorders in Adolescents: Korean Validity and Reliability Study

Se-Hoon Shim,Jong-Hun Lee,Jye-Heon Song,Beomwoo Nam,Bo-Hyun Yoon,Ha-young Jin,Hyung-Mo Sung,Jong Hyun Jeong,Sae-Heon Jang,Duk-In Jon,Young Sup Woo,Won-Myong Bahk 대한정신약물학회 2018 CLINICAL PSYCHOPHARMACOLOGY AND NEUROSCIENCE Vol.16 No.3

Objective: This study aimed to evaluate the validity and reliability of a Korean version of the Mood Disorder Questionnaire-Adolescent version (K-MDQ-A) as a screening instrument for bipolar disorders in adolescents. Methods: One hundred two adolescents with bipolar disorders and their parents were recruited from November 2014 to November 2016 at 7 training hospitals. One hundred six controls were recruited from each middle school in two cities of South Korea. The parent version of the original MDQ-A was translated into Korean. The parents of all participants completed the K-MDQ-A. The diagnoses of bipolar disorders were determined based on the Korean version of K-SADS-PL. The test-retest reliability with a 10-month interval was investigated in 33 bipolar adolescents. Results: K-MDQ-A yielded a sensitivity of 0.90 and a specificity of 0.92 when using a cut-off score of endorsement of 5 items, indicating that symptoms occurred in the same time period and caused moderate or serious problems. The internal consistency of the K-MDQ-A was good. The correlations between each item and the total score ranged from 0.40 to 0.76 and were all statistically significant. Factor analysis revealed 3 factors that explained 61.25% of the total variance. The mean total score was significantly higher in bipolar adolescents (7.29) than in controls (1.32). The Pearson correlation coefficient for the total test-retest score was 0.59 (p<0.001). Conclusion: The K-MDQ-A completed by parents showed the excellent validity and reliability and may be a useful screening tool for adolescents with bipolar disorders attending in- and outpatient psychiatric clinics.

장애인의 정보격차 해소 방안에 대한 연구

이근민,김인서,임명준 대구대학교 특수교육재활과학연구소 2004 再活科學硏究 Vol.22 No.1

본 연구의 목적은 장애인의 정보격차를 해소하기 위한 방안에 대한 연구를 하고자 함에 있다. 정보화가 진행되면서 장애인의 컴퓨터 이용 현황은 비장애인와 비교하였을 때 낮은 수준에 머물러 있다. 먼저 정부의 노력을 법률, 정책, 산업 그리고 보급 및 서비스 전달체계로 나누어 그 문제점과 개선방안을 기술하였다. 장애인 정보격차 해소 방안으로 세가지 모델을 제시하였다. 첫째, 전문적인 교육체계 수립 및 전문가 양성이 필요하다. 둘째, 체계적인 컴퓨터 대체 접근 서비스 제공을 위한 개선 방안을 제시 하였다. 세 번째로 장애인 고용을 위한 장애인 정보화 지원센터의 미래상을 보여줬다. The purpose of this study is to provide plans to reduce digital divide for people with disabilities. As the digital world advances, computer utilization rate for persons with disabilities is lower compared to the non-disabled population. Efforts by central government along with its problems and improvement plans are presented according to legislation, policy, industry, distribution and service delivery system. In order to reduce digital divide, 3 plans are proposed as follow: ① educational system and professional training in the field of digital must be established; ② improvement plan for providing systematic alternative computer access service has been proposed; ③ establish and expand digital support center for the employment of disabled.