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Bacillus sp. YA-14의 Xylanase 와 β- Xylosidase유전자의 재조합
나규흠,김지만,박희경,배동훈,유주현 한국산업미생물학회 1990 한국미생물·생명공학회지 Vol.18 No.2
토양으로부터 분리한 알카리내성 Bacillus sp. YA-14의 xylanase 유전자를 포함하고 있는 재조합 plasmid pYDC21의 EcoRI site에 β-xylosidase 유전자를 함유한 plasmid pYXL22의 5.7kb DNA 단편을 삽입시킴으로써 xylanase와 β-xylosidase 유전자가 동시에 연결된 약 13kb의 재조합 plasmid pYDK43을 제조하였다. Xylanase와 β-xylosidase를 동시에 생산하는 재조합 균주 E. coli HB101(pYDX43)이 기질인 xylan으로부터 직접 xylose까지 분해하는 것을 TLC상에서 확인하였다. E. coli HB101(pYDX43)이 생산하는 xylanase의 경우 공여균주인 Bacillus sp. YA-14보다 효소생산이 약 30% 증가하였으나 β-xylosidase의 경우 2.5배 감소하였다. A recombinant plasmid, pYDX43, was consturcted by inserting 5.7kb EcoRI DNA fragment of pYXL22 containing β-xylosidase gene into EcoRI site of pYDC21 which was carrying encoding xylanase gene. The end-product of xylan hydrolysis with crude cell extract of E. coli HB101 harboring pYDX43 was found to be xylose by TLC analysis. The productivity of xylanase with E. coli HB101(pYDX43) was 1.3 times higher than that with Bacillus sp. YA-14, but the synthesis of β-xylosidase was rather 2.5 times lower.
나규흠,김학주,김계원,김기태,이정환,양중익 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.4
Tobramycin 고생산성 변이주를 쉽고 신속하게 선별할 수 있는 screening 방법을 개발하였다. 고농도의 apramycin이 포함된 배지를 사용함으로서 nebramycin 생산성이 낮은 변이주들을 1차적으로 제거할 수 있었다. 모균주인 S. tenebrarius ATCC 17920에는 저지환을 나타내지 못하고 생산성이 향상된 변이주의 경우에만 저지환을 형성하는 strain No.23을 토양으로부터 분리하여 Ps. paucimobilis로 동정하였고 1차 선별된 변이주들 중 tobramycin 생산성이 높은 균주들을 선별하기 위한 피검균으로 사용하였다. 이러한 screening 전략으로 strain No.23에 명확히 저지환을 나타내는 변이주 58주를 얻었고, HPLC를 이용하여 각 변이주의 tobramycin 생산성을 비교 측정한 결과 모균주에 비해 3∼8배 생산성이 향상되었음을 확인하였다. An improved method for the selection of high tobramycin-producing mutants of Streptomyces tenebrarius ATCC 17920 was investigated. By the use of apramycin-containing media, low nebramycin-producing mutants were eliminated. Strain No.23, resistant to apramycin and kanamycin B and sensitive to tobramycin, was isolated from soils, identified as Pseudomonas paucimobilis and used as a test organism for overlaying the mutants on agar plate. While inhibition zones were not shown when the parent strains were overlaid with soft agar containing the strain No. 23, clear zones were shown when high tobramycin-producing mutants were overlaid. Using this screening strategy, 58 mutants showing clear zones had been isolted, antibiotic activities of which were incresed to 3-8 fold compared to that of parent strain.
( Tae Heum Park ),( Woo Il Kim ),( Na Gyeong Yang ),( Kyu Rak Hong ),( Young Lip Park ),( Sung Yul Lee ),( Moon Kyun Cho ),( Sooyoung Kim ) 대한피부과학회 2020 대한피부과학회 학술발표대회집 Vol.72 No.1
Background: Green tea extract (GTE) has been studied for the treatment of acne based on its anti-inflammatory/ antioxidant properties. Objectives: This systematic review and meta-analysis aimed to examine the effects of GTE on acne. Methods: Electronic databases, including PubMed, Embase, and the Cochrane Library were systematically searched up to August 2019. Results: The effect size of acne lesion counts is presented as mean differences and 95% confidence intervals (CIs). Five randomized-controlled studies were included in the meta-analysis (N; experimental=125, control=122). GTE significantly reduced the number of inflammatory lesions (-9.38; 95% CI: -14.13 to -4.63). In subgroup analysis, topical GTE application significantly reduced the inflammatory lesion counts (-11.39; 95% CI: -15.91 to -6.86) whereas oral GTE intake showed minimal effect (-1.40; 95% CI: -2.50 to -0.30). Although GTE did not significantly reduce the number of non-inflammatory lesions (-21.65; 95% CI: -47.52 to 4.22), when stratified by the route of admission, non-inflammatory acne lesions were significantly reduced by topical GTE application (-32.44; 95% CI: -39.27 to -25.62) but not with oral GTE administration (0.20; 95% CI: 0.00 to 0.40). Conclusion: This systematic review and meta-analysis suggest that topical GTE application is beneficial for the treatment of acne without causing significant adverse events while oral GTE intake has limited effects. Further high-quality clinical trials are warranted.
Categorized wetland preference and life forms of the vascular plants in the Korean Peninsula
Choung, Yeonsook,Min, Byeong Mee,Lee, Kyu Song,Cho, Kang-Hyun,Joo, Kwang Yeong,Hyun, Jin-Oh,Na, Hye Ryun,Oh, Hyun Kyung,Nam, Gi-Heum,Kim, Jin-Seok,Cho, Soyeon,Lee, Jongsung,Jung, Sangyeop,Lee, Jaeyeon The Ecological Society of Korea 2021 Journal of Ecology and Environment Vol.45 No.1
Background: In 2020, a categorized list of wetland preferences, major habitats, and life forms of 4145 vascular plant taxa occurring in the Korean Peninsula was published by the National Institute of Biological Resources. We analyzed the list and explored the distribution patterns of the five categorized groups according to wetland preference, along with the information on the major habitats and the life forms of the plants belonging to those categories. Results: Out of 4145 taxa, we found that 729 wetland plant taxa (18%) occur in Korea: 401 obligate wetland plants and 328 facultative wetland plants. Among the 729 wetland taxa, the majority (73%) was hygrophytes and the remaining 27% was aquatic macrophytes. Furthermore, almost all of the wetland taxa are herbs; so, woody plants are only 4.7%. The 16 carnivorous taxa distributed in Korea were characterized as obligate wetland plants. Conclusions: We expect the categorized information would promote understanding of the characteristics of the plant species and would be an important source for understanding, conservation, and restoration of wetland ecosystems.
-경혈 및 트리거 포인트에서- 미약 자기장을 이용한 근피로 회복 평가
김수병,이나라,심태규,이승욱,이용흠,Kim, Soo-Byung,Lee, Na-Ra,Shim, Tae-Kyu,Lee, Seung-Wook,Lee, Yong-Heum 한국정보통신학회 2010 한국정보통신학회논문지 Vol.14 No.5
이에 본 연구에서는 비침습적인 방식으로 국소적 부위인 경혈과 Trigger Point에서 경결된 근조직과 신경조직을 자극할 수 있는 자기장 자극 시스템을 제작하였다. 시스템을 이용하여 가정용 저주파치료기기와 동일한 조건에서 상완 이두박근의 근피로 유발 후 근피로 회복을 평가하였다. 경혈과 Trigger Point의 해부학적 위치가 동일한 청영혈(HT2)를 자극위치로 선정하였다. 등척성 운동을 통하여 25명의 일반인에게 근피로를 유발시켰으며, 무자극 5명, 저주파 치료기기의 전기자극 10명, 자기장 자극 10명으로 분류하였다. 자극시간은 5분으로 하여 자극하였으며, 5일동안 EMG를 측정하여 관찰하였다. EMG를 주파수영역에서 분석해본 결과, 무자극의 근피로 회복률이 가장 저조했으며, 저주파치료기기의 전기자극보다 자기장 자극이 근피로 회복에 효과적인 것으로 확인되었다. The aim of this study was developing the non-invasive focused magnetic stimulation system(NI_FMSS) that can stimulates acupoints which are small parts and knotted muscular tissues. To evaluate pain treatment effect of the NI_FMSS, we caused muscle fatigue to 25 subjects in biceps of the arm. Then, we stimulated acupoints(trigger points) HT2 using low frequency stimulator(10 subjects) and NI_FMSS(10 subjects). The other 5 subjects had not been stimulated. We analyzed muscle fatigue recovery with median frequency, RMS and median power in frequency domain for 5 days. We checked the magnetic stimulation effect on acupoint by evaluation of muscle fatigue recovery. Therefore, we identified that the NI_FMSS was more efficient system to relieve muscle pain than electric-stimulation system.
E. coli ATCC 21990이 생산하는 Aminoglycoside-3'-Phosphotransferase ( APH(3') )의 Densitometric TLC Assay
이정환,김계원,김기태,나규흠,김학주,양중익,김수일 한국산업미생물학회 1991 한국미생물·생명공학회지 Vol.19 No.4
E. coli ATCC 21990의 aminoglycoside-3'-phosphotransferase(APH(3'))의 신속하고 간편한 정량적 방법을 TLC densitometry를 이용하여 확립하였다. APH(3') 반응생산물인 3' 위치에 인산화된 kanamycin B(3'-PKMB)는 silica gel plate에서 chloroformmethanol-method-ammonia water(3:4:3) 전개용매로 반응물에서 분리되었고, 3'-PKMB의 양은 ninhydrin으로 발색 후 densitometry로 측정하였다. APH(3')의 densitometric TLC assay는 좋은 정량적 결과와 재현성을 보였고, 3'-PKMB에 대한 감도는 1.56nmol이었으며 많은 시료의 분석이 한번의 실시로 가능하였다. 이 방법은 aminoglycoside 항생제의 불활화 효소 분석에 응용이 가능하리라 여겨진다. A rapid and simple quantitative assay method for aminoglycoside-3'-phosphotransferase(APH(3')) derived from E. coli ATCC 21990 was developed using the thin layer chromatographic densitometry, 3'-phosphorylated kanamycin B(3'-PKMB), product of APH(3') reaction, was separated from reaction mixtures by developing on the silica gel TLC plate with chloroform-methanol-ammonia water (3:4:3). The quantity of the 3'-PKMB was measured by densitometry after color development by ninhydrin method. Densitometric TLC assay for APH(3') was showed a good quantitative result and reproducibility. Sensitivity of this assay was 1.56 nmol of 3'-PKMB and could be analyzed many samples at same time. This method may be applicable for the analysis of inactivating enzymes of aminoglycoside antibiotics.