기체 크로마토그래피를 이용한 유기산 및 아미노산의 동시분석에 관한 연구

金京禮,金正漢,朴炯國,鄭恩枝 성균관대학교 약학연구소 1993 成均藥硏論文集 Vol.5 No.1

N(O, S)-isobutyloxycarbonylation with subsequent solid-phase extraction and tert.-butyl-dimethylsilylation was described for the simultaneous gas chromatographic analysis of 19 protein and 12 nonprotein amino acids and 26 organic acids from aqueous samples. Linear responses in the range of 10∼100 ppm were obtained for the most of compounds studied with the correlation coefficient varying from 0.993 to 0.999. When the present method was applied to wine samples, 13 organic acids and 13 amino acids were screened simultaneously.

병풀(Centella asiatica) 엑스-베타시클로텍스트린 고체 분산체를 함유한 폴록사머 407 히드로겔 제조 및 피부투과

김경국,곽은선,이계원,박진규,박목순,지웅길 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-

Extract of Centella asiatica(ECA), which is poorly water-soluble extract from the Centella asiatica is known to express excellent wound healing properties. ECA-β-cyclodextrin (asiaticoside-β-cyclodextrin and genin-β-cyclodextrin) solid dispersion system, which was prepared by freeze-drying method, was formulated as gels containing poloxamer 407 and propylene glycol, and evaluated with respect to their viscosity. stability, skin permeation and drug amount in the skin of hairless mouse. The average molar ratio asiaticoside-β-CD and genin-β-CD was 1:1,7 and 1:22, respectively. When the molar ratio of genin and β-CD was 1:5, madecassic acid made 100% solid dispersion system and asiatic and about 65%. In dissolution study, >99% of asiaticoside from asiaticoside-β-CD was dissolved in 5 minutes, and >99% madecassic acid and >64% asiatic acid from genin-β-CD. The apparent viscosity of poloxamer 407 gels with ECA-β-CD solid dispersion system increased in proportion to poloxamer 407 and gels showed that asiaticoside was most stable and madecassic acid stable and asiatic acid similar to stability of gel with free ECA. The permeation amount of asiaticoside in poloxamer gels through-hairless mouse skin decreased as the concentration of poloxamer 407 increased. When propylene glycol was added at the level of 10%, the permeation amount of asiaticoside at poloxamer gels through hairless mouse skin increased but from 15% it decreased. The permeation of asiaticoside into the skin of hairless mouse was estimated to be about 0.10 ㎍/㎠.

악교정 수술에서 모의 조종된 3차원 전산화 단층촬영상의 응용

김형돈,이경상,유선국,박창서 大韓口腔顎顔面 放射線學會 1998 Imaging Science in Dentistry Vol.28 No.2

In orthodontics and orthognathic surgery, cephalogram has been routine practice in diagnosis and treatment evaluation of craniofacial deformity. But its inherent distortion of actual length and angles during projecting three dimensional object to two dimensional plane might cause errors in quantitative analysis of shape and size. Therefore, it is desirable that three dimensional object is diagnosed and evaluated three dimensionally and three dimensional CT image is best for three dimensional analysis. Development of clinic necessitates evaluation of result of treatment and comparison before and after surgery. It is desirable that patient that was diagnosed and planned by three dimensional computed tomography before surgery is evaluated by three dimensional computed tomography after surgery, too. But Because there is no standardized normal values in three dimension now and three dimensional Computed Tomography needs expensive equipments and because of its expenses and amount of exposure to radiation, limitations still remain to be solved in its application to routine practice. If postoperative three dimensional image is constructed by pre and postoperative lateral and postero-anterior cephalograms and preoperative three dimensional computed tomogram, pre and postoperative image will be compared and evaluated three dimensionally without three dimensional computed tomography after surgery and that will contribute to standardize normal values in three dimension. This study introduced new method that computer-simulated three dimensional image was constructed by preoperative three dimensional computed tomogram and pre and postoperative lateral and postero-anterior cephalograms, and for validation of new method, in four cases of dry skull that position of mandible was displaced and four patients of orthognathic surgery, computer-simulated three dimensional image and actual postoperative three dimensional image were compared. The results were as follows. 1. In four cases of dry skull that position of mandible was displaced, range of displacement between computer-simulated three dimensional images and actual postoperative three dimensional images in co-ordinates values was from -1.8 mm to 1.8 mm and 94% in displacement of all co-ordinates values was from -1.0 mm to 1.0 mm and no significant difference between computer-simulated three dimensional images and actual postoperative three dimensional images was noticed(p>0.05). 2. In four cases of orthognathic surgery patients, range of displacement between computer-simulated three dimensional images and actual postoperative three dimensional images in coordinates values was from -6.7 mm to 7.7 mm and 90% in displacement of all co-ordinates values was from -4.0 to 4.0 mm and no significant difference between computer-simulated three dimensional images and actual postoperative three dimensional images was noticed(p>0.05). Conclusively, computer-simulated three dimensional image was constructed by preoperative three dimensional computed tomogram and pre and postoperative lateral and postero-anterior cephalograms. Therefore, potentiality that can construct postoperative three dimensional image without three dimensional computed tomography after surgery was presented.

Gas Chromatography를 이용한 Aspergillus niger KCCM 32005의 Myrcene 대사산물에 대한 연구

金京禮,崔文禎,李尙駿,朴炯國,金正漢 성균관대학교 약학연구소 1991 成均藥硏論文集 Vol.3 No.1

Abstract-After biotransformation of myrcene with Aspergillus niger KCCM 32005, its neutral metabolites were isolated from the conversion broths and analyzed by GC and GC/MS. Myrcene was converted into various terpenoids. Among them the five compounds, which were considered to be important as flavor, were identified as follows; terpinen-4-ol. α-terpineol, trans-carveol, cis-carveol, and carvone. Higher production was accomplished when the amount of myrcene was kept at lower but steady concentration by portion-wise introduction to the culture broth.

대구지역 일부 중학생의 건강증진행위와 관련요인

김경희,한창현,권진희,이성국 韓國保健敎育.健康增進學會 2001 보건교육건강증진학회지 Vol.18 No.2

The propose of this study is to find out how well the middle school students are practicing the health Promotion behaviors and the factors relating their health promotion bahavior. Questionnaire survey on 922 middle school students attending 6 middle schools (three middle schools for each sex) located in Taegu City from the 7th through 19th of Feb. 2000 were conducted. The following were as follows; 1.The perceived health status is higher in male students than in female students(p<0.01). And the ratio of the students' feeling that they are healthy becomes also high in proportion to their economic status, and their mothers' educational level, and their parents' interest in health(p<0.01). The perceived importance of health is high in proportion to the student's economic status, and their parent's interest in health. 2.In case of the Health Locus of Control in Personality, the students with both parents have higher trend of inner control than the students with single mother or single father. The perceived self efficacy is significantly higher in male students than in female students(p<0.01). And it becomes significantly high in proportion to the students' economic status and their parents' educational level and interest in health(p<0.01). It is also higher in the students who had no diseases. 3.In case of the perceived benefit of the health promotion behavior, the ratio of the students responding that it is high is higher in male students than in female students(p<0.01). It also becomes high in proportion to the students' economic status, and their parent's educational level and interest in health(p<0.01). The barriers of the health promotion behavior was found to have no variables that are related to itself. 4.According to the data from Multiple Regression of Analysis which has the health promotion behavior practice as a subordinate variable, in male students' case the degree of health promotion behavior practice becomes high in promotion to their parents' interest in health, and the perceived health status. Their degree of health promotion behavior practice is also in proportion to their perceived self efficacy and the perceived benefit of the health promotion behavior practice. But in case of the barriers of the health promotion behavior practice, the result is the opposite. As to the female students, their health behavior practice becomes high in proportion to their parents' interest in health. It also becomes high in proportion to the perceived health status, the understanding of the importance of the health, the perceived self efficacy and the perceived benefit of the health promotion behavior. But in case of the barriers of the health promotion behavior, it was the same as the male students' case.

종격동 종괴를 의심케한 우대동맥궁 1례

김만우,백학연,정춘해,장경식,홍순표,조건국,김종진 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Developmental anomalies of the aortic arch, commonly known as vascular rings, are relatively rare congenital vascular anomalies which can compress the trachea and esophagus. One routine chest film of a patient who was checked and hepatoma-diagnosed in Internal Medicine, Chosun University Hospital left the room for doubt of mediastinal mass. But no subjective symptoms for the mass was told by the patient. This is to report our experience of a patient having right side aortic arch with aberrant left subclavian artery, as proved by diagnosis, but none of congenital heart disease. This right side aortic arch with aberrant left subclavian artery is the most frequent type among right aortic arch, the anomaly of which is usually detected from the incidental suspicion for mediastinal mass at routine chest film; because no symptoms are accompanied therewith.

성인형 당뇨병 환자에서 피부생검을 이용한 표피하 모세혈관 기저막 두께의 변화에 관한 연구

김원학,오금탁,김만우,장경식,채종구,조건국 朝鮮大學校 附設 醫學硏究所 1987 The Medical Journal of Chosun University Vol.12 No.1

Thickening and proliferation of capillary basement membrane is a generalized phenomenon in diabetes mellitus and has been described in many organs including heart, kidney, pancreas, retina etc. While such changes are specific, it is difficult to obtain specimens from those organs. We took the specimens from the medial surface of the thing of 33 diabetics and 4 healthy persons as controls by means of punch biopsy. The measurement carried out by normogram and electron microscopic technique. HbA_(1c) values were also measured simultaneously. 1. The HbA_(1c) values are higher in diabetics than in control group (p<0.01). 2. The subepidermal capillary basement membrane thickness of the diabetics : 30% of the 5 th decade population, 53.9% of the 6 rh decade population and 83.3% of the 7th decade population showed more rhan 3,000 A. Whereas all the controls showed below 3,000 A. 3. The subepidermal capillary basement membrane thickness did nor increase significantly with the duration of the disease. 4. In cases of high subepidermal capillary basement membrane thickness, HbA_(1c) showed significant increase. (p<0.01).

수면관련 호흡장애 환자의 MMPI 및 SCL-90-R 반응 특성

김경우,윤석준,양창국,한홍무 한국정신신체의학회 2002 정신신체의학 Vol.10 No.1

Bifidobacterium angulatum KCTC3236의 α-galactosidase에 의한 Oligo당 생성의 최적조건

왕지원,김경민,강대중,강국희,정재록 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Bifidobacterium angulatum KCTC3236 showed the highest α-galactosidase activity among the tested strains of Bifidobacteria( data not shown). Optimum culture conditions for the maximum production of α-galactosidase were as follows. The culture media was MRS broth containing 1% galactose as a carbon source and its initial pH was 7.0. The incubation temperature and time were 37℃, 21 hours respectively. Melibiose have been used as substrate. The formation of oligosaccharides was maximum at 40℃ when the substrate concentration was 30%. And the unit of α-galactosidase was 40 unit. Finally, we could observe the maximum production of oligosaccharides after 14 hours' enzyme reaction.

상악동에 발생한 국균증 2례

조용석,김경원,박현진,강지연,이건국,신향미 충북대학교 의학연구소 2001 忠北醫大學術誌 Vol.11 No.1

국균증은 토양, 유기체 그리고 모든 형태의 유기물 잔사에 광범위하게 존재하는 국균 (Aspergillus) 속에 의하여 발병한다.이 균의 포자는 공기 중에서 전염될 수 있으므로 가장 흔한 감염 진균 중 하나이다.부비동에 발생한 국균증은 초기 임상 증상이 만성 부비동염과 유사하며, 방사선 사진 등을 이용한 검사상도 일반 세균성 감염과 구별하기가 매우 어렵다.대개는 병리조직학적 검사로 국균을 확인해서 확진을 내리게된다.국균증의 치료는 외과적으로 상악동내의 병소를 완전히 제거하고 비강에로 충분한 공기를 통기시키는 것이 가장 중요하며 보조적으로 전신적인 항진균제를 사용해야한다. 증례는 40세와 24세인 여자 환자들로서 전신적으로는 건강했으며 임상적 방사선적으로 만성 상악동 증세를 보였다.상악동 근치술을 시행하면서 국균종으로 여겨지는 암갈색 덩어리들이 관찰되었고 술 후 병리조직검사상 국균증으로 진단되었다.국균증으로 진단 후 보조적으로 전신적 항진균제를 투약하였으며 재발없이 치유되었다. Aspergillus is a fungus of the Acetomyces class that is most commonly encountered in the human environment.Aspergillosis of the maxillary sinus is not common and it requires special consideration to diagnose and eradicate. The disease is characterized by a wide range of initial symptoms, and should be considered as a possible diagnosis in sinusitis refractory to antibiotics and antral lavage. Generally the diagnosis of sinus aspergillosis can be confirmed by histologic examination, however, characteristic radiographic appearance such as coherene of radiodense sinus concretions can be found by careful examination of the radiograms. The choice of treatment of maxillary sinus aspergillosis is radical surgery with adjunctive systemic antifungal therapy. We experienced two cases of maxillary sinus aspergillosis, which initially diagnosed as chronic maxillary sinusitis and showed a necrotic mass intraoperatively and histologically diagnosed as aspergillosis.We reports theses cases with review of literature.