Substrate specificity of a recombinant d-lyxose isomerase from Providencia stuartii for monosaccharides

Kwon, H.J.,Yeom, S.J.,Park, C.S.,Oh, D.K. Society for Bioscience and Bioengineering, Japan ; 2010 Journal of bioscience and bioengineering Vol.110 No.1

The specific activity and catalytic efficiency (k<SUB>cat</SUB>/K<SUB>m</SUB>) of the recombinant putative protein from Providencia stuartii was the highest for d-lyxose among the aldose substrates, indicating that it is a d-lyxose isomerase. Gel filtration analysis suggested that the native enzyme is a dimer with a molecular mass of 44 kDa. The maximal activity for d-lyxose isomerization was observed at pH 7.5 and 45 <SUP>o</SUP>C in the presence of 1 mM Mn<SUP>2+</SUP>. The enzyme exhibited high isomerization activity for aldose substrates with the C2 and C3 hydroxyl groups in the left-hand configuration, such as d-lyxose, d-mannose, l-ribose, d-talose, and l-allose (listed in decreasing order of activity). The enzyme exhibited the highest activity for d-xylulose among all pentoses and hexoses. Thus, d-lyxose was produced at 288 g/l from 500 g/l d-xylulose by d-lyxose isomerase at pH 7.5 and 45 <SUP>o</SUP>C for 2 h, with a conversion yield of 58 % and a volumetric productivity of 144 g l<SUP>-1</SUP> h<SUP>-1</SUP>. The observed k<SUB>cat</SUB>/K<SUB>m</SUB> (920 mM<SUP>-1</SUP> s<SUP>-1</SUP>) of P. stuartiid-lyxose isomerase for d-xylulose is higher than any of the k<SUB>cat</SUB>/K<SUB>m</SUB> values previously reported for sugar and sugar phosphate isomerases with monosaccharide substrates. These results suggest that the enzyme will be useful as an industrial producer of d-lyxose.

미국 의학드라마에 나타난 간호이미지와 역할수행 연구

권영애,길민지,김정현,박옥경,서민정,유현정,이민경,정지혜,조양현,천기윤,이자형,정덕유,김성남 이화여자대학교 간호과학대학 2009 이화간호학회지 Vol.- No.43

We tried to inquire the people's views on nursing or nurses, correct the image of nurse and take it as a basis to be applied on nursing education examining the image of nursing on Television drama playing a important role of mass media. 523 nurses of the characters in drama is applied to the analysis object of this study by selecting 3 American medical dramas, Grey's Anatomy, House M.D, E.R from 2005 to 2007. Contents analysis method was used in Data Analysis, which was used in Lim Mi-lim ?Research on contents analysis on image of nursing on television drama?, 7 Items was used after Coders previously modify and compensate it based on research documents of Kalish & Kalisch(1986), Yoon Young(1983), Lee Hye-Ok(1982), Lee Young-Bok, etc in analysis items. Before making the coding, we did pre-test. 6 Coders made of 3teams, 2 Coders are in each team, made the Codings the article on each person by Item seeing the recorded Film. An each team makes coding about 50episodes, total 147episodes for 2weeks. We used double coding and when the results are different between two coders, we decided just one result through mutual agreement. The average of reliability degree was 97.4% which measured the reliability degree by the method of Holsti. The statistic method of Frequency, Percentage was used using EXCEL Program in data processing. The result of research is as follows. 1. The general feature of nurses on the drama is that they are women(92.7%) which is 20 or 30-aged(70.5%). 2. Relative importance of 65.2% nurses in drama is depicted as surrounding characters and most of them was expressed as the character plating surrounding role. 3. The affair attitude of nurses shown on drama is revealed as active(24.5%), autonomous(32%), kind(16.8%), mechanical(23.2%) and adept(28.4%). However, unknown category is the highest in all affairs. 4. The professional activity of nurses was classified with professional/simple affair, The professional activity of nurses was 80% and simple activity of nurses was 22.9%. The professional activity of nurses was classified with personal/assistant affair. The personal activity of nurses was 61.9% and assistant activity of nurses was 15.1%. 5. The place nurses of the drama appears are hospital(97.7%). 6. The appearance feature of nurses is shown on common physique (55.0%), common stature(79.3%), common appearance(76.1%), common dignity(97.5%) and decent (51.1%). 7. The feature of character of nurses depicted on Television drama is neutral in all 11 affairs. We want the following epigraph based on above conclusion. 1. The continuos research intended for mass people is required about how their image of nurse is changed after watching medical drama. 2. The research to construct monitoring mass media system and make plans for developing is required for nurse image being in a triangular position as a professional job.

Potential roles of D-serine and serine racemase in experimental temporal lobe epilepsy

Ryu, H.J.,Kim, J.-E.,Yeo, S.-I.,Kim, D.-S.,Kwon, O.-S.,Choi, S.Y.,Kang, T.-C. Wiley Subscription Services, Inc., A Wiley Company 2010 Journal of neuroscience research Vol.88 No.11

<P>To confirm the roles of D-serinergic gliotransmission in epilepsy, we investigated the relationship between spatiotemporally specific glial responses and the D-serine/serine racemase system in mesial temporal structures following status epilepticus (SE). In control animals, D-serine and serine racemase immunoreactivities were detected mainly in astrocytes. After SE, D-serine and serine racemase immunoreactivities were increased in astrocytes. Double-immunofluorescence study revealed that up-regulation of serine racemase immunoreactivity was relevant not to D-serine immunoreactivity but to nestin or vimentin immunoreactivity. Neither D-serine nor serine racemase was found in naïve or reactive microglia. In addition, phosphorylated N-methyl-D-aspartate (NMDA) receptor subunit 1 (pNR1-Ser896) immunoreactivity in the hippocampus was increased compared with controls. Increased D-serine immunoreactivity showed direct correlation with the phosphorylation of Ser896 of NR1. Given the findings of our previous study, these findings suggest that D-serine and serine racemase in astrocytes may play roles in neuronal hyperexcitability via a cooperative activation of NMDA receptors. Furthermore, serine racemase may be involved in migration and differentiation of immature astrocytes, which is relevant to reactive astrogliosis. © 2010 Wiley-Liss, Inc.</P>

Drastic anthocyanin increase in response to PAP1 overexpression in fls1 knockout mutant confers enhanced osmotic stress tolerance in Arabidopsis thaliana

Lee, W. J.,Jeong, C. Y.,Kwon, J.,Kien, V.,Lee, D.,Hong, S. W.,Lee, H. Springer Science + Business Media 2016 Plant cell reports Vol.35 No.11

<P>pap1 - D/fls1ko double mutant plants that produce substantial amounts of anthocyanin show tolerance to abiotic stress. Anthocyanins are flavonoids that are abundant in various plants and have beneficial effects on both plants and humans. Many genes in flavonoid biosynthetic pathways have been identified, including those in the MYB-bHLH-WD40 (MBW) complex. The MYB gene Production of Anthocyanin Pigment 1 (PAP1) plays a particularly important role in anthocyanin accumulation. PAP1 expression in many plant systems strongly increases anthocyanin levels, resulting in a dark purple color in many plant organs. In this study, we generated double mutant plants that harbor fls1ko in the pap1-D background (i.e., pap1-D/fls1ko plants), to examine whether anthocyanins can be further enhanced by blocking flavonol biosynthesis under PAP1 overexpression. We also wanted to examine whether the increased anthocyanin levels contribute to defense against osmotic stresses. The pap1-D/fls1ko mutants accumulated higher anthocyanin levels than pap1-D plants in both control and sucrose-treated conditions. However, flavonoid biosynthesis genes were slightly down-regulated in the pap1-D/fls1ko seedlings as compared to their expression in pap1-D seedlings. We also report the performance of pap1-D/fls1ko seedlings in response to plant osmotic stresses.</P>

Forebrain-specific ablation of phospholipase Cγ1 causes manic-like behavior

Yang, Y R,Jung, J H,Kim, S-J,Hamada, K,Suzuki, A,Kim, H J,Lee, J H,Kwon, O-B,Lee, Y K,Kim, J,Kim, E-K,Jang, H-J,Kang, D-S,Choi, J-S,Lee, C J,Marshall, J,Koh, H-Y,Kim, C-J,Seok, H,Kim, S H,Choi, J H,Ch Macmillan Publishers Limited, part of Springer Nat 2017 Molecular psychiatry Vol.22 No.10

<P>Manic episodes are one of the major diagnostic symptoms in a spectrum of neuropsychiatric disorders that include schizophrenia, obsessive-compulsive disorder and bipolar disorder (BD). Despite a possible association between BD and the gene encoding phospholipase C gamma 1 (PLCG1), its etiological basis remains unclear. Here, we report that mice lacking phospholipase C gamma 1 (PLC gamma 1) in the forebrain (Plcg1(f/f); CaMKII) exhibit hyperactivity, decreased anxiety-like behavior, reduced depressive-related behavior, hyperhedonia, hyperphagia, impaired learning and memory and exaggerated startle responses. Inhibitory transmission in hippocampal pyramidal neurons and striatal dopamine receptor D1-expressing neurons of Plcg1-deficient mice was significantly reduced. The decrease in inhibitory transmission is likely due to a reduced number of gamma-aminobutyric acid (GABA)-ergic boutons, which may result from impaired localization and/or stabilization of postsynaptic CaMKII (Ca2+/calmodulin-dependent protein kinase II) at inhibitory synapses. Moreover, mutant mice display impaired brain-derived neurotrophic factor-tropomyosin receptor kinase B-dependent synaptic plasticity in the hippocampus, which could account for deficits of spatial memory. Lithium and valproate, the drugs presently used to treat mania associated with BD, rescued the hyperactive phenotypes of Plcg1(f/f); CaMKII mice. These findings provide evidence that PLC gamma 1 is critical for synaptic function and plasticity and that the loss of PLC gamma 1 from the forebrain results in manic-like behavior.</P>

Effect of Supplementation of Bacillus subtilis LS 1-2 Grown on Citrus-juice Waste and Corn-soybean Meal Substrate on Growth Performance, Nutrient Retention, Caecal Microbiology and Small Intestinal Morphology of Broilers

Sen, Sinol,Ingale, S.L.,Kim, J.S.,Kim, K.H.,Kim, Y.W.,Khong, Chou,Lohakare, J.D.,Kim, E.K.,Kim, H.S.,Kwon, I.K.,Chae, B.J. Asian Australasian Association of Animal Productio 2011 Animal Bioscience Vol.24 No.8

A feeding trial was conducted to investigate the effect of dietary supplementation of Bacillus subtilis LS 1-2 grown on citrus-juice waste and corn-soybean substrate on growth performance, nutrient retention, caecal microbial population and intestinal morphology in broilers. Three hundred twenty d-old Ross chicks were randomly allotted to 4 treatments on the basis of BW in a randomized complete block design. Each treatment had 4 replicates of 20 chicks in each. Experimental diets were fed in 2 phases, starter (d 0 to 21) and finisher (d 21 to 35). Dietary treatments were; negative control (NC: basal diet without any antimicrobial), positive control (PC: basal diet added with 20 mg/kg Avilamycin), basal diet added with 0.30% Bacillus subtilis LS 1-2 grown on corn-soybean substrate (P1), and basal diet added with 0.30% Bacillus subtilis LS 1-2 grown on citrus-juice waste substrate (P2). Overall BW gain, feed intake and FCR were better (p<0.05) in PC, P1 and P2 treatments as compared to NC. Moreover, overall BW gain and FCR in PC and P2 treatments were greater than P1. Retention of CP, GE (d 21, d 35) and DM (d 35) were increased (p<0.05) in treatments PC, P1 and P2 compared with NC. At d 35, caecal Clostridium and Coliform counts were lower (p<0.05) in treatments PC, P1 and P2 than NC. Moreover, Clostridium and Coliform counts in treatment PC was lower (p<0.05) than P1. Villus height and villus height to crypt depth ratio in both duodenum and ileum were increased (p<0.05) in treatments PC, P1, P2 as compared to NC. However, retention of nutrients, caecal microbial population and intestinal morphology remained comparable among treatments P1 and P2. It is concluded that Bacillus subtilis LS 1-2 inclusion at 0.30% level had beneficial effects on broilers' growth performance, nutrient retention, caecal microflora and intestinal morphology. Additionally, citrus-juice waste can be used as substrate for growth of probiotic Bacillus subtilis LS 1-2.

Comparative Efficacy of Different Soy Protein Sources on Growth Performance, Nutrient Digestibility and Intestinal Morphology in Weaned Pigs

Yang, Y.X.,Kim, Y.G.,Lohakare, J.D.,Yun, J.H.,Lee, J.K.,Kwon, M.S.,Park, J.I.,Choi, J.Y.,Chae, B.J. Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.5

To elucidate the efficacy of different soy protein sources on piglet's performance, a total of 280 weaned piglets ($Duroc{\times}Yorkshire{\times}Landrace$, $23{\pm}3$ d of age, $5.86{\pm}0.45$ kg initial BW) were allotted to 5 treatment diets comprising soybean meal (SBM), soy protein concentrate (SPC), Hamlet protein (HP300), fungal (Aspergillus oryzae) fermented soy protein (FSP-A), and fungal plus bacterial (A. oryzae+Bacillus subtilis) fermented soy protein (FSP-B), respectively. Experimental diets for feeding trial were formulated to contain each soy protein sources at 8% level to corn-whey powder basal diet. There were 14 pigs per pen and 4 pens per treatment. Experimental diets were fed from 0 to 14 d after weaning and then a common commercial diet was fed from 15 to 35 d. Also for ileal digestibility studies, 18 pigs were assigned to 6 dietary treatments as N-free, SBM, SPC, HP300, FSP-A and FSP-B with T-canulation at distal ileum for 6 days. At $14^{th}$ d of experimental feeding, the ADG was significantly higher (p<0.05) in SPC fed diet as compared with others. Similar trend was noticed during the 15-35 d and overall study (0-35 d). All the processed soy protein sources tested in this experiment improved (p<0.05) growth than SBM during overall study. The nutrient digestibility of GE, DM, CP and Ca showed lower (p<0.05) values in SBM and FSP-A fed groups than SPC and FSP-B treatments. The apparent ileal digestibility of TEAA, non-TEAA and TAA showed lower (p<0.05) in SBM treatments compared with other soy protein sources. The true ileal digestibility of TEAA, non-TEAA and TAA were lower (p<0.05) in SBM fed group than SPC and HP300 treatments, and lower than FSP treatments though they didn't achieve significant difference (p>0.05). Villous height and crypt depth was not affected by dietary treatments. In conclusion, the growth and digestibility of nutrients in weaned pigs fed SPC was superior to others. Also FSP-A and FSP-B showed improved performance than those fed SBM.

環境 汚染에 關한 硏究 : 大氣汚染에 關하여 about Air Pollution

權赫姬,金東君,崔德一,李哲遠,尹錫根,金鎭仁 國立保健硏究院 1973 국립보건연구원보 Vol.10 No.-

Sintering temperature dependent piezoelectric properties of (Bi,Na)TiO₃-(Ba,Sr)TiO₃ ceramics

Kwon, Y.H.,Shin, D.J.,Lee, G.H.,Koh, J.H. Ceramurgica ; Elsevier Science Ltd 2016 CERAMICS INTERNATIONAL Vol.42 No.8

In this paper, the strain and piezoelectric properties of lead-free piezoelectric (100-x)(Bi<SUB>0.5</SUB>Na<SUB>0.5</SUB>)TiO<SUB>3</SUB>-x(Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>)TiO<SUB>3</SUB> (x=0, 3, 6, 8, 12, 15, 18wt%) ceramics were investigated for sintering temperatures ranging from 1125 to 1225<SUP>o</SUP>C to determine the optimized processing conditions. The optimized (100-x)(Bi<SUB>0.5</SUB>Na<SUB>0.5</SUB>)TiO<SUB>3</SUB>-x(Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>)TiO<SUB>3</SUB> ceramics exhibited a high relative dielectric permittivity ε<SUB>r</SUB> of 1608, piezoelectric charge coefficient d<SUB>33</SUB> of 155pC/N, electromechanical coupling factor k<SUB>p</SUB> of 38.5%, and the generated electric power of 25.7nW/cm<SUP>2</SUP>, respectively. Especially, the electric field dependent strains of (100-x)(Bi<SUB>0.5</SUB>Na<SUB>0.5</SUB>)TiO<SUB>3</SUB>-(Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>)TiO<SUB>3</SUB> ceramics were also measured and analyzed, and various types of strain effects were observed and analyzed. Our studies suggest that optimized sintering temperature and optimized barium strontium titanate content can enhance the piezoelectric properties of lead-free (100-x)(Bi<SUB>0.5</SUB>Na<SUB>0.5</SUB>)TiO<SUB>3</SUB>-x(Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>)TiO<SUB>3</SUB> ceramics. As a result, the optimized composition was 88(Bi<SUB>0.5</SUB>Na<SUB>0.5</SUB>)TiO<SUB>3</SUB>-12(Ba<SUB>0.5</SUB>Sr<SUB>0.5</SUB>)TiO<SUB>3</SUB>, which sintered at 1200<SUP>o</SUP>C.

간호대학생의 이론・실습 성적과 임상수행능력의 관련성 : 성인간호학 교과목을 중심으로

권현진,김다영,김민정,김소현,박예담,박지원,왕서현,유영주,이재현,주가은,신수진 이화여자대학교간호학회 2020 이화간호학회지 Vol.- No.54

Purpose: This study aimed to explore the relationship among score of theory course, score of practicum course and clinical practice ability by the course, Adult Health Nursing. Methods: A descriptive research was conducted using questionaries. Data were collected from September 25 to October 2, 2019. Participants were 67 nursing students at one university in Seoul on their junior year who have experienced clinical settings. The collected data were analyzed by t-test, ANOVA, Cohen’s Kappa, and Scheffe’s test using SPSS 25.0 version program. Results: Participants were given a quartile number from 1 to 4 respectively according to their scores of theory course those of practicum course. The total mean score of clinical practice ability was 3.61, with a perfect score of 5. The scores of theory course and practicum course showed slight correlation(Cohen’s Kappa=.168). However, significant correlation did not existed among score of theory course, score of practicum course and clinical practice ability. Conclusion: The result showed that score of theory course, score of practicum course and clinical practice ability have no significant correlation. However, its finding suggests the necessity of further research about various approach and new evaluation methods needed in order to improve nursing students’ competency.