부산 백병원에서 실시한 개심술의 최근 기법

조광현 인제대학교 백병원 2002 仁濟醫學 Vol.23 No.1S

■ Background 3,175 cardiac surgeries were performed in the Pusan Paik Hospital from Sep. 1985 to April 2002, and recently several kinds of current techniques for open heart surgery were adopted with good results. ■ Method Retrospective analysis of the cases and review of the results of operation and usefulness of current techniques such as OFF-Pump CABG. ARCH-FIRST TECHNIQUE. MICRO-WAVE ABLATION, TRANSPLANTATION and MINIMAL INCISION were done. ■ Results ① Among the total cases (3,179), open heart and non-open heart surgery cases were 1,988 and 218, respectively. ② 188 cases of CABG were done. Among them, 20 cases underwent OFF-PUMP coronary artery bypass surgery. 21 cases underwent complete arterial revascularization, and 14 cases underwent both. ③ 75 cases of aortic aneurysm (ascending or arch aneurysm) were operated undercardiopulmonary bypass. The causes of these aneurysm were dissecting aneurysm (45), annuloaortic ectasia(24), non-dissecting chronic aneurysm(5). In the surgery of arch aneurysm. 7 cases underwent ARCH-FIRST TECHNIQUE with good results. ④ In the treatment of atrial fibrillation coupled with valvular heart disease. MICRO-WAVE ABLATION were performed in 18 cases with 83.3 % sinus rhythm recovery. ⑤ Four cases of heart transplantation were performed, using Shumway method, with 3 survivals and one death. ⑥ Minimally invasive incision (using lower sternal incision) was performed successfully in the cases of tricuspid valve diseases(10) and congenital septal defects (50). ■ Conclusion The over all postoperative hospital mortality with open heart surgery was not so high as 3.5 %, whereas more proper management of the all patients with current techniques must be continued.

肝組織에서의 Glucagon의 脂肪酸合成 抑制機轉에 대한 硏究

曺義鉉,高光三 조선의대 1984 The Medical Journal of Chosun University Vol.9 No.1

심장수술 2,450예와 심장이식

조광현,황윤호,이양행,류지윤 인제대학교 1999 仁濟醫學 Vol.20 No.1

원예작물의 염류장애 원인과 대책을 위한 생리, 생화학적 연구 3.토마토 뿌리조직에서 마이크로솜 Ca²+ uptake 특성연구

조광현,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1998 연구보고서 Vol.3 No.-

식물세포의 세포질 Ca²+ 이동과 관련된 Ca²+ transport 특성을 조사하기 위하여, 토마토 뿌리조직으로부터 마이크로솜을 분리하고, 45Ca²+ uptake 실험을 수행하였다. 반응용액에 Ca²+-ATPase의 선택적 저해제인 1 mM vanadate와 액포막 H+-ATPase의 선택적 저해제인 50 mM NO₃를 각각 첨가하였을 때, 45Ca²+ uptake는 각각 20%와 30% 저해되었고, 두 가지 저해제를 동시에 첨가하였을 때 50% 저해되었다. 또한 마이크로솜 45Ca²+ uptake는 vanadate 또는 NO₃-의 농도를 증가시킴에 따라 저해되었다. 이러한 저해효과는 토마토 뿌리조직 마이크로솜에 Ca²+-ATPase와 Ca²+/H+ antiport가 동시에 존재할 가능성을 시사한다. Protonophore인 gramicidin의 처리에 의해 45Ca²+ uptake는 30% 가량 저해되어, 토마토 뿌리조직 마이크로솜에 Ca²+/H+ antiport가 존재하고 있음을 확인하였다. NO₃-를 처리하여 45Ca²+ uptake를 저해시킨 후 gramicidin을 처리하였을 때, gramicidin에 의한 추가저해는 거의 관측되지 않았으나, vanadate의 경우 gramicidin에 의한 추가저해가 현저히 나타났다. 이러한 결과는 토마토 뿌리조직 마이크로솜 45Ca²+ uptake가 vanadate에 의하여 저해되는 Ca²+-ATPase와 NO₃-에 의하여 저해되는 Ca²+/H+ antiport에 의해 이루어지고 있음을 의미한다. 한편, 동물조직 ER/SR-type Ca²+-ATPase의 특이성 높은 선택적 저해제인 thapsigargin은 마이크로솜 45Ca²+ uptake를 농도 의존적으로 저해하였으며, 10μM 농도에서 최대저해효과를 나타냈다. Thapsigargin에 의한 45Ca²+ uptake의 저해효과는 NO₃를 사용하여 액포막 H+-ATPase 활성을 저해하였을 때 현저하게 감소하였다. 반면, vanadate를 처리하여 원형질막 H+-ATPase의 활성을 저해한 후에도 thapsigargin에 의한 추가적 저해효과가 관측되어 thapsigargin이 액포막 H+-ATPase의 활성을 저해하여 간접적으로 Ca²+/H+ antiport를 저해함을 확인하였다. 이상의 결과들은 토마토 뿌리조직 마이크로솜에 Ca²+-ATPase와 액포막 Ca²+/H+ antiport가 존재하며, thapsigargin이 액포막 Ca²+/H+ antiport를 선택적으로 저해함을 보여준다. In order to characterize the property of Ca²+ transport in plant tissue, microsomes were prepared from the roots of tomato and microsomal 45Ca²+ uptake was measured. When 1mM vanadate, a selective inhibitor of P-type ATPases, 50 mM NO₃, a specific inhibitor of vacuolar H+-ATPase, and both of these inhibitors were treated, the microsomal 45Ca²+ uptakes were inhibited by 20%, 33%, and 47%, respectively. The inhibitory effects of these two inhibitors were investigated by using a protonophore, gramicidin. When the chemical gradient of H+ was relieved by gramicidin, the uptake was decreased by 30%, implying the presence of Ca²+/H+ antiporter in the microsomal membrane. In the 45Ca²+ uptake experiment, the effect of gramicidin was independent of vanadate-induced inhibition. However, when the activity of vacuolar H+-ATPase was inhibited by NO₃, the effect of gramicidin was severely decreased. Meanwhile, thapsigargin, a specific antagonist of ER/SR-type Ca²+-ATPase, inhibited the microsomal 45Ca²+ uptake and the maximum inhibitory effect was obtained at 10μM. The effect of thapsigargin was blocked by NO₃and gramicidin, but not by vanadate. These results imply that vanadate directly inhibits the activity of Ca²+-ATPase; however, NO₃- and thapsigargin block the activity of Ca²+/H+ antiporter by inhibiting the vacuolar H+-ATPase. In conclusion, the microsomal 45Ca²+ uptakes are mediated by two major enzymes, Ca²+-ATPase and Ca²+/H+ antiporter in tomato root tissue.

CAD/CAM 으로 제작된 Ceramic Inlay의 변연누출에 관한 비교연구

조병훈,이명종,임미경,이광원,손호현 大韓齒科保存學會 1997 Restorative Dentistry & Endodontics Vol.22 No.2

To compare the marginal leakage of CAD/CAM-fabricated ceramic inlay, gold inlay and amalgam, forty extracted caries-free premolars were prepared with Class Ⅱ MO cavity design. The teeth were divided into four groups of ten samples each. Group 1 was restored with CAD/CAM-fabricated ceramic inlays cemented with Scotchbond Resin Cement / Scotchbond Multi-purpose plus. Group 2 was restored with gold inlays cemented with Scotchbond Resin Cement / Scotchbond Multi-purpose plus. Group 3 was restored with gold inlays cemented with zinc phosphate cement. And, Group 4 was restored with amalgam. All samples were thermocycled, and stored in 1% methylene blue. Marginal leakage was examined at four margins, that is, occlusal distal, priximal gingival, occlsal facial and occlusal lingual margins from sectioned samples under steeomicroscope(x15). The results were as follows: 1. Group 1 and 2 showed no statistically significant difference among marginal leakage at all four examined margins(p>0.05). 2. Group 3 and 4 showed significant marginal leakage at proximal gingival margin compared with other margins(p<0.05). 3. Significantly increased marginal leakage at priximal gingival, occlusal facial and occlusal lingual margins in group 3 were observed compared with other groups(p<0.05).

황토 급여가 거세한우의 발육에 미치는 영향

조원모,기광석,정하연,강수원,김준식,백봉현,김용국 한국국제농업개발학회 2003 韓國國際農業開發學會誌 Vol.15 No.1

본 연구는 거세한우 육성우에 대한 황토 첨가급여가 발육에 미치는 효과를 구명하고자 발육능력, 사료섭취량을 규명한 바 아래와 같은 결과를 얻었다. 전 시험기간 동안의 평균 일당증체량은 대조구와 황토급여구가 각각 0.91㎏과 0.87㎏/day로 대조구가 높은 것으로 나타났지만 유의성은 인정되지 않았다. 사료섭취량은 대조구와 황토급여구 모두 평균 일일 두당 농후사료 섭취량이 2.42㎏이었으나, 건초섭취량에 있어서는 각각 3.02와 3.14㎏으로 황토급여구가 다소 높은 경향으로 나타났다. The study was conducted to investigate the effect of dietary supplements of reddish clay on the growth performance in growing Hanwoo steers. A total of 8 calves was allocated into 2 feeding groups, control(basal diet) and treatment(basal diet plus ad libitim Reddish clay) for Hanwoo steers. This study was carried out for 5 months with a total of 8 calves. The results obtained in the present studies are summarized as follows : Average daily gains throughout the experimental period of 4 months to 9 months of age were 0.91 and 0.87㎏ for control group and reddish clay-supplemented group, respectively, however, no statistical significances were found. Average daily intake of concentrates was 2.42㎏ for each group, while that of hay tended to be higher in reddish clay-supplemented group(3.14㎏) than in control group(3.02㎏).

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

γ-PGA에 포함된 D-glutamate의 효소화학적 정량분석

조현정,지광환 금오공과대학교 2005 論文集 Vol.26 No.-

Bacillus subtilis chungkookjang produces a new biofunctional polymer, poly y-glutamic acid (γ-PGA). γ-PGA consists of D and/or L-glutamate and its ratio is different depending microorganisms and culturing environrrents, Here we are reporting the new enzymatic method to determine the stereochemistry of D-glutamic acid composition ratio in γ-PGA D-amino acid aminotransferase (D-AAT) can catalyzes transamination between D-amino acids and a-keto acids. Thus the amount of D-glutamate in γ-PGA can be determined by the assaying the amount of produced D-alanine followed by the reaction of D-AAT in the presence of pyruvic acid. D-glutamate and D-alanine can be separated by thin layer chromatography (TLC) in NH₄0H : ethanol (2:8, v/v) solvent system D-alanine can be visualized by ninhydrin (0.5% in 75% ethanol) spraying and quantitated by the measurement the absorbance of TLC spot followed by the elution with 0.005% copper sulfate in 75% ethanol at EOOnm. Partially purified D-AAT is good enough to carry out this new and very convenient method to determine the amount of D-glutamic acid in γ-PGA.

광학적 측정을 이용한 정밀 스테이지 위치오차의 실시간 측정 방법에 관한 연구

조웅,현광익,김종형 한국공작기계학회 2008 한국공작기계학회 춘계학술대회논문집 Vol.2008 No.-

This paper suggests method to measure that error of the precision stage is used precision process and measurement equipment by using camera optics. Though laser interferometer measures position error of stage when precision stage is manufactured by us or it is used to measure stage for the nonce as a general rule, optics tooling position measurement method to suggest is possible to apply in real-time and trace an error range continually. Therefore it is able to improve capabilities of equipment by compensating an error range when operates precision process and measurement equipment in real-time.