포도의 재배 및 저장기간 중의 Procymidone 및 Chlorothalonil의 잔류량변화

고광용,김금희,이규승 한국환경농학회 2004 한국환경농학회지 Vol.23 No.1

In order to know the residual pattern of pesticides and predict to the degradation period until below MRL, we experimented procymidone and chlorothalodi for grape which were the most detected pesticide in grape by NAQS(National Agricultural product Quality management Service) survey. In this experiment, we sprayed those pesticides 10 days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10 day sample to establish logical equation and to calculate DT_(50) Also the same day samples stored at 4℃ and 20℃, which were compared their degradation patterns. During the cultivating period, the residue amount of procymidone was changed from 1.85㎎/㎏(0day) to 0.33㎎/㎏(10days), DT_(50) was 3.5 days, and chlorothalonil was changed from 5.5 ㎎/㎏ (0 day) to 3.49 ㎎/㎏ (10 day), DT_(50) was 4.4 days. During the storage period, DT_(50) of procymidone and chlorothalonil at 49c were 10.5 and 7.6 days, and 6.3 and 6.1 days at 20℃, respectively.

쪽파[Allium ascalonicum L.] 재배기간 중 Diazinon, Iprodione 및 Chlorfenapyr의 잔류랑 변화

고광용,나은식,김성헌,김상준,장영희,이규승 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.2

In order to know the residual pattern of pesticides and predict to the degradation period until below MRL, we experimented diazinon, iprodinon and chlorfenapyr for shallot. They were the most detected pesticide in shallot by NAQS (National Agricultural product Quality management Service) survey. In this experiment, we sprayed those pesticides 15days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10, 14 day sample to establish logical equation and to calculate DT_(50). During the cultivating period, the residue amount of diazinon was changed from 1.02 mg/kg (0 day) to 0.01 mg/kg (14 day), DT_(50) was 2.19 days, and iprodione was changed from 1.45 mg/kg (0 day) to 0.14 mg/kg (14 day), DT_(50) was 4.15 days, and chlorfenapyr was changed from 1.5 mg/kg (0 day) to 0.01 mg/kg (14 day), DT_(50) was 1.97 days. The DT_(50) of double amount in those pesticides, diazinon was 2.24 days, iprodione was 4.82 days, and chlorfenapyr was 2.24 days, respectively.

Swivel joint를 이용한 비대칭 발치증례의 교정적 공간폐쇄

고창희,임성훈,김광원,정동기,김도영,박선주,양용근 조선대학교 구강생물학연구소 2003 口腔生物學硏究 Vol.27 No.1

Extraction therapy is popular treatment protocol in orthodontics. Generally, symmetric premolar extraction is performed. But, in some cases, asymmetric extraction is required to solve asymmetric dental alignment. But asymmetric extraction treatment has side effects and disadvantages like a) collapse of dental arch form. b) canting of occlusal plane, c) extended treatment duration. For a good treatment result, we applied the orthodontic space closing arch wire with swivel joint to allow rotational movement of arch wire segments during space closure, Now we present a case treated with orthodontic space closing arch wire with swivel joint.

들깻잎이 재배 및 저장기간중 Procymidone 및 Bifenthrin의 잔류량변화

고광용,이용재,원동준,박혜진,이규승 한국환경농학회 2003 한국환경농학회지 Vol.22 No.1

들깻잎에서 procymidone과 bifenthrin의 경우 안전사용기준인 약제살포 후 5일에 각각의 농도가 38.16, 1.77 mg/kg 이지만 이는 살포 조건과 재배환경에 따른 농약의 초기부착농도가 높은데서 기인한 것이라고 예상되어진다. 앞에서의 결과들로 미루어 볼 때 일반적인 농약의 안전사용기준으로 농약을 처리한다면 최초살포 이후 재배기간에서 5일간 50% 이상 이 분해되고 10일 후에는 90%이상이 분해된다. 또한 상온저장 중 20~40%가 분해되며 수돗물 세척에 의해서 만으로도 50%이상이 제거되므로 조리과정을 감안하지 않더라도 초기살포량의 약 90%는 분해 또는 제거되어 안전사용기준에 의거하여 사용한다면 두 약제 모두 MRL이하로 잔류하게 되어 별다른 문제가 없을 것으로 예상되어진다. 하지만 현행법상 수확 시점에 그 잔류량을 조사하도록 되어 있으므로 깻잎의 경우 재배가 대부분 하우스에서 이루어짐을 감안한다면 두 약제 모두 초기살포시 작물체로서의 부착량이 많을 경우에 수확시점에서의 잔류량이 MRL을 상회할 가능성도 있음을 알 수 있었다. 따라서 이에 따른 약제 사용량의 축소나 저농도 약제의 사용, 살포일수의 조정 등에 관한 연구가 필요하다고 본다. In order to know residual pattern of pesticides and to predict degradation period until below MRL, we experimented with procymidone and bifenthrin for perlua leaf which were the most detected pesticides by NAQS (National Agriculture-product Quality management Service) survey. In this experiment, we sprayed those pesticides 10 days before harvest and analyzed 0, 1, 2, 3, 5, 7, 10 day samples to establish logical equation and to calculate DT_(50). Also degradation patterns of those samples were compared during storage at 4℃ and 20℃. During cultivating period, procymidone residue amount was changed from 79.52 mg/kg (0 day) to 4.2 mg/kg, DT_(50) was 2.65 days by logical-equation, and bifenthrin residue amount was changed from 5.03 mg/kg (0 day) to 0.17 mg/kg, DT_(50) was 2.24 days. During storage period, DTso of procymidone and bifenthrin at 4℃ was 12.23 days and 10.57 days, and at 20℃ was 6.32 days and 8.2 days, respectively.

자궁 경부에서 발생한 상피내 암종과 침윤성 암종의 간질 변화에 관한 병리조직학적 연구

고성민,노광을,이민전,김용임,이미자,전호종,서재홍 조선대학교 1993 The Medical Journal of Chosun University Vol.18 No.2

Connective tissue seems to be involved in malignancy by lytic processes as a crucial element in invasive growth. This looks relatively simple, but data have emerged that the stroma is not just a passive but rather an active participant. Recent advances in the borderland between cancer and connective tissue research have increasingly made it clear that the relationship between malignant tissue and its stroma is a very intricate one. The present study was performed in order to investigate the variety of stromal reactivity and alterations of basement membrane accompanying malignant growth and the distribution of Langerhans cells and T and B lymphocytes in cervical epithelium affected by intaepithelial neoplasia and invasive cervical carcinoma. The subjects in this were 14 cervicitis, 42 cervical intraepithelial neoplasia, 14 microinvasive cervical carcinoma and 14 invasive cervical carcinomas. A total of 84 formalin-fixed and paraffin-embedded specimens of normal, inflamed and neoplastic uterine cervix have been studied in order to correlate the epithelial changes with the expression of α-smooth muscle actin, S-100 protein, α_1-antichymotrypsin, lysozyme, LCA, CD20, UCHL1, OPD4, CD1, CD4, CD8, CD68 and typeⅣ collagen in stroma. The results of immunohistochemical and electron microscopical examinations yield virtually identical findings 1) The number of α-smooth muscle actin positive cells and the intensity of stain were related to the increasing grading of cervical intraepithelial neoplasia and invasive cervical carcinoma. 2) Normal cervix and cervical intraepithelial neoplasia grade Ⅰ and Ⅱ showed continuous basement membrane but invasive cervical carcinoma showed highly variable basement membrane deposition ranging from continuous to almost completely absent. 3) The cervical intraepithelial neoplasia Ⅲ and invasive cervical carcinoma showed an increase in spindle shaped Langerhans cells associated with increased numbers of stroma and intraepithelial lymphoid cells. The evaluation of collagen Ⅳ in basement membrane, S-100 protein and CD1 in Langerhans cells and α-smooth muscle actin in stromal cells of the uterine cervix may be an useful adjunct to diagnostic criteria of cervical intraepithrlial neoplasia and invasive cervical carcinoma, and may help understanding of the mechanisms of mesenchymal epithelial interactions during neoplasia.

PWM을 이용한 화재 감지용 자외선 센서의 구동 방법

고낙용,최한수,김광진,임병현 조선대학교 에너지.자원신기술연구소 2004 에너지·자원신기술연구소 논문지 Vol.26 No.1

We propose driving method of Ultraviolet sensor for fire alarms using pulse width modulation that is used to fire detector. Ultraviolet sensor is UV Tron using gas multiplication effect to current discharge and photoelectric effect of metal. To have high sensibility and to gain proper output voltage. it should have highly responsive performance. This research designed a driving circuit with UV sensors and proposed a method for false alarm reduction. The result shows the prevention and extinction of fire technique degree, certificated operation of detector.

복숭아의 재배 및 저장기간 중 Procymidone, Chlorpyrifos 및 Cypermethrin의 잔류량 변화

이용재,고광용,원동준,길근환,이규승 한국환경농학회 2003 한국환경농학회지 Vol.22 No.3

복숭아의 생산단계에서 잔류허용 기준과 최종 소비단계에서의 잔류양상 평가자료로 활용하기 위하여 복숭아에서의 procymidone, chlorpyrifos 및 cypermethrin의 포장상태에서와 실온(20°C) 및 저온(4°C) 저장시 잔류양상을 알아보았다. Procymidone의 포장상태에서의 반감기는 표준량 3.1, 배량 3.4일로 나타났고, chlorpyrifos는 포장상태에서 포준량 7.2, 배량 5.8일로 나타났으며, cypermethrin은 표준 10.1, 배량 14.4일로 나타났다. 약제 살포 후 초기 부착량이 MRL 이하로 내려갈 때까지의 시간을 계산해 본 결과 배량으로 살포 할 경우 procymidone과 chlorpyrifos는 안전사용기준 보다 시간이 오래 걸렸지만, 표준량으로 사용할 경우 별다른 문제가 없는 것으로 나타났다. 저장기간 중의 procymidone, chlorpyrifos, cypermethrin의 반감일수는 20°C에서 4.6, 10.2, 12.9일 이며, 4°C에서는 16.1, 14.3, 13.1일로 저온에서의 반감기가 실온에서보다 더 긴 것을 알 수 있었다. 한편 세척방법에 의한 잔류농약의 제거율은 수돗물에서는 최고 procymidone 82.9%, chlorpyrifos 27.0%, cypermethrin 24.0%로 나타났고, 세제를 사용 할 경우 procymidone 88.8%, chlorpyrifos 59.0%, cypermethrin 59.4%의 제거율을 보였다. The residue patterns of procymidone, chlorpyrifos and cypermethrin in peaches were examined. The pesticides were sprayed at 15 days before harvest and then were determined the residue at 0, 1, 2, 3, 5, 7, 10 and 15 days after pesticide application and calculated their DT_(50). Also, the degradation patterns at 4℃ and 20℃ during storage period were compared. Biological half-lives of procymidone, chlorpyrifos and cypermethrin in peaches during the cultivation period were 3.1, 7.2 and 10.4 days, respectively. The biological half-life of procymidone was shorter than the others. During the storage period, half-lives of procymidone, chlorpyrifos and cypermethrin were 16.0, 14.3 and 13.1 days at 4 C and 4.6, 10.2 and 12.9 days at 20℃, resp ectively. The degradation rates of these three pesticides in storage period were slower than them in cultivation period. Removal rates were 22.2--82.9% by tap water, and 12.5∼88.8% by detergent solution.

도심 교차로 노면 지표유출수의 초기수질특성에 관한 연구

최상용,고광백,이지영,임세호 한국환경과학회 2006 한국환경과학회지 Vol.15 No.4

합성피혁제조업체 근로자들의 요중 N-Methylformamide 농도의 계절적 변이

이광영,변주현,송혜란,김진하,고광욱,이용환 大韓産業醫學會 2003 대한직업환경의학회지 Vol.15 No.2

목적: 본 연구는 여름과 겨울의 계절적 차이에 따른 디메틸포름아미드의 체내 흡수의 변화를 알아보고자 시행되었다. 방법: 일개 합성피혁제조업체에서 직접적으로 디메틸포름아미드를 취급하는 22명의 근로자들을 대상으로 2001년 2월(겨울)과 7월(여름)에 작업환경측정을 통해 기중 DMF농도를 측정하였고 특수건강검진을 실시하여 요중 NMF의 배설량을 측정하고 간기능 검사를 시행하였다. 또한 검사 당일의 평균 기온과 평균 습도를 측정하고 설문지를 통해 알코올 음주 정도, 피부 질환의 유무, 보호구 착용 유무 등을 조사한 후 여름과 겨울의 자료를 비교 분석하였다. 결과: 겨울과 여름의 평균 공기 중의 디메틸포름아미드 기하 평균 농도는 각각 11.55ppm, 13.78ppm으로 통계적으로 유의한 차이는 없었으나 요중엔-메틸포름아미드 배설량의 기하 평균은 겨울이 31.23㎎/g creatinine인 것에 비해 여름이 96.09㎎/g creatinine으로 겨울에 비해 여름의 요중 엔-메틸포름아미드 배설량이 유의하게 높았다(p<0.05). 공기 중 디메틸포름아미드 농도(ppm)에 대한 요중 엔-메틸포름아미드 배설량 (㎎/g creatinine)의 비는 여름(1:6.97)이 겨울(1:2.70)에 비해 약 2.6배 정도 높았다. 결론: 여름과 겨울의 요중 NMF배설량의 차이는 아마도 연구 대상 사업장의 환기 시설이 미약하고 근로자들이 DMF에 적합한 보호구를 적절하게 사용하지도 않았기 때문이고, 무엇보다도 높은 온도와 습도에서 짧은 옷을 입고 근무함으로써 피부 노출면적이 증가되어 DMF의 피부 흡수의 양이 많아졌기 때문으로 판단된다. Objectives: This study was carried out to identify seasonal variations of urinary concentrations of N-methylformamide(NMF) among workers employed at a synthetic leather factory. Methods: Study subjects consisted of 16 male and 6 female workers who were involved in the direct treatment of dimethylformamide(DMF) in a synthetic leather factory. By using health examination data and the results of air measurements and biologic monitoring conducted in February and July, 2001, we identified seasonal variations of the DMF concentrations in the air and NMF concentrations in urine. Results: 1) In winter and summer, average temperatures at the working sites were 3.2℃ and 26.5℃, respectively and average humidities were 35.4% and 84.5%, respectively. 2)Airborne DMF concentrations were not significantly different between summer(13.78ppm) and winter(11.55ppm). 3)NMF concentrations in urine were found to be significantly higher in summer(96.09㎎/g creatinine) than in winter(31.23㎎/g creatinine) (p<0.001). Conclusions: The seasonal difference in the urinary excretion values of NMF may be due to increased dermal absorption of DMF with the higher ambient temperature and humidity in summer and the increased area of exposed skin.

모시조개 배지에서의 장내세균 및 비브리오균 증식

김용훈,고광균,이강순 순천향대학교 1992 논문집 Vol.15 No.2

This study was performed to develop a culture medium which fovors the growth of Vibrio vulnificus of the extract with a short-necked clam(Cyclina sinesis). The new plating medium made of the extract of a short-necked clam was tested for the growth of Vibrio vulnificus and a members of Enterobacteriaceae as well as Staphylococcus aureus and their abilities to form colonies in the media were compared with these to form colonies in the medium. The following results were obtained 1. The Vibrio vulnificus grew well and formed large colonies on the Cyclina sinensis agar plate and yeast-extract peptone agar plate. The Cyclina sinensis agar medium was better than the thiosulfate-citrate0bile salt-sucrose(TCBS) agar for the isolation of Vibrio vulnifificus. 2. All the bacteriae of Enterobacteriaceae family grew well on the Cyclina sinensis agar and the Brain Heart Infusion agar plate but the growth was inhibited on the MacConkey agar. 3. The growth of Staphylococcus aureus was much inhibited on the Cyclina sinensis agar media.