종설(綜說) : 한의학 학술지에 발표된 탈모에 관한 연구현황 분석

김광중 ( Kwang Joong Kim ),백승엽 ( Seung Yeob Baek ),임채광 ( Chae Kwang Im ) 대구한의대학교 제한동의학술원 2012 東西醫學 Vol.37 No.4

We performed a systemic analysis of the research situations on hair and alopecia published in the journal of oriental medicine to understand the search trend in the area of hair and alopecia, prepare the basis for the interests and basis on hair and alopecia in the oriental medicine area and above that, to support for the applications in the clinics through this and came to the following conclusions. 1. There are total 95 publications in the domestic journals of oriental medicine area from 1986 to 2011. 2. Total 95 articles were published in 23 journals. “Dong-eui journal of physiology and pathology” had the largest number of articles which was 22(23%) followed by “Oriental journal of ophthalmology, otolaryngology, and dermatology” of 19(20%). 3. The authors who published the largest number of articles as the first author were “Cheon-jong Kim”, “Hyeon-taek Shin”, and “Tae-hu Lee” who published 3 each, and the correspondent author who published the largest number of articles was “Chang-hyeon Lee” who published 8 articles. 4. For the contents and methodologies of the study, there were 16 literature reviews, 36 clinical trials, and 43 animal and other experimental studies and 3 literature reviews were published in 2002, 6 animal and other experimental studies were published in 2006, and 6 clinical trials were published in 2004, 2006, 2009, and 2010 each the most. Based on this study, we hope not only scientific treatises but also these for the oriental medicines and alopecia study related to the oriental medicines to be continued.

경락(經絡)과 정신양생(精神養生)의 관계 설정(設定)에 대(對)한 고찰(考察)

백진웅,김광호,Baek Jin-Woong,Kim Kwang-Ho 대한예방한의학회 2001 대한예방한의학회지 Vol.5 No.2

It is thought that Kim Hong-kyeong followed the existing theory of Meridian(經絡) and supplemented his unique perspectives in interpreting it. And he tried to relate Meridian with hygiene of mind. I took up with the research on the assumption that the review of Kim's method can present new perspectives to the study of relationship between Meridian and hygiene of mind. The results of my study are as follows: 1. Kim followed common theory of Meridian and then added his unique perspectives to it. His method of interpretation has one characteristic. By introducing Idealism(唯心論), he understood 'Meridian' as the path of the mind and the emotion. Especially I think his hypothesis that acupuncture can be widely used in mental diseases as well as body related diseases and its applications to treatment are very valuable. 2. Meridian and hygiene of mind are close together. I think Kim's unique trial is something new in studying relationship between Meridian and hygiene of mind but requires more research on it.

흉부외상 없이 발생한 흉부대동맥 절단증의 조기 진단 : 2례 보고 Two Cases Report

한승백,전영진,백광제,김준식,김정택,김광호,선경 大韓應急醫學會 1998 대한응급의학회지 Vol.9 No.4

Aortic transection or interruption is a rare condition which developed after an acute deceleration injury. Its occurrence depends on the location and direction of the force applied and is usually from motor vehicle accident or falling down. The exact incidence of aortic transection in trauma is not known but, when develops, only about 10-15% of the victims can survive and be transported to the hospital. Even in the survivors, majority of them will be fatal within a few days if a prompt diagnosis and surgical treatments are not made. Aggressive diagnostic work-up is recommended for the patients with high suspicious index, which would salvage the victims with this fatal condition. We report the experience of two cases of aortic transection or interruption following motor vehicle accidents.

晉州 附近 新洞層群(下部 白堊系)에서 産出되는 化石 Stromatolite의 生堆積 構造 및 古環境

金榮汶,白光錫,徐承祚 晋州敎育大學校 科學敎育硏究所 1998 科學敎育硏究 Vol.24 No.-

For the purpose of clarifying the organosedimentary structure and paleoenvironment of stromatolites from the southem part of the Kyongsang Basin, the study was carried out both through the field works and chemical analysis of samples. Obtained data were compared with the repotred ones from areas in North Kyongsang Province, additionally. Through this study, the paleaoenvironments of these areas were summarized as follows: 1. Laminations of stromatolite are consist of algal-rich and sediment-rich laminae altemately. The content of calcite, main component of stromatolite, has less Magnesium value. Thus, it seems to have formed under the fresh water environment. 2. The almost of the stromatolites have very irregular shapes which has been caused by disturbed and divided algae from microbial activities. Therefore, it can be inferred that the lake was calm. 3. It seems to have been shallow lacustrine environment and intermittent dry season.

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

학생증 인식을 이용한 웹 환경에서의 학사 관리 시스템

김광백 新羅大學校 2003 論文集 Vol.52 No.-

This paper proposes a method which recognizes student's identification card by using image processing and recognition technology and can manage student information on the web. The presented scheme sets up an average brightness as a threshold, based on the most bright pixel and the least bright one for the source image of the ID card. It is convert to binary image, applies a horizontal histogram, and extracts school number through its location. And, it removes the noise of the school number region, each number is extracted using vertical histogram and normalized. Each recognition of school number is done by the ARTI algorithm. Experiment results show that the proposed scheme can efficiently extract the region of school number and recognize its letter. So, it can effectively manage student information through a database of the recognized characters.

새로운 PCR 클로닝 벡터의 개발

김윤일,백광희,윤재승 경희대학교 산학협력기술연구원 1996 산학협력기술연구논문집 Vol.2 No.-

PCR (Polymerase Chain Reaction) is a powerful technique for amplifying specific DNA region of interest. The PCR was used first only in the field of molecular biology, but nowadays it is widely used in the various fields such as diagnosis of genetic disorders, identification of forensic samples, and classification of organisms. However, Taq(Thermus aquaticus)DNA polymerase used in PCR has been reported to add a single nontemplate-directed A residue to the 3' ends of amplified KNA, giving rise to the difficulties in the cloning of amplified DNA. Considering the wide applications of the PCR, the development of the efficient cloning vector for PCR-amplified DNA would be very helpful for various purposes, we have designed the PCR cloning vector using the nucleotide sequence recognized by restriction endonuclease Xcm I. The double incorporation of Xcm I recognition sites in the pUC 19 and m13 DNA can make compatible ends carrying T residue and be easily ligated to PCR products.

농산 폐기물인 Allium속 뿌리를 이용한 Ni와 Pb 이온 제거

김성조,백승화,김운성,문광현 한국식품영양학회 1998 韓國食品營養學會誌 Vol.11 No.6

농산물의 갈무리시 발생되는 건조한 마늘, 쪽파 및 대파 뿌리 분말을 흡착제로 이용하여 수용액 중 이온 상태로 존재하는 Ni과 Pb의 흡착력을 시료의 입자별, 중금속의 농도별, 용액의 온도별, pH별로 분석하였다. 중금속 이온들은 흡착제의 입자크기가 작을수록 흡착률이 높았고, 그중 마늘뿌리가 Pb에 대해 높은 흡착력을 나타냈다. 수용액중 중금속 농도가 높을수록 중금속들이 흡착되는 양이 증가하였고, 흡착률은 흡착제와 중금속의 종류에 따라 차이가 있었다. 온도의 증가는 쪽파와 대파에서 Ni과 Pb가 감소되었다. 알칼리 조건에서 비교적 흡착이 잘 되는 중금속은 Ni이며, 중성과 산성조건에서는 Pb의 흡착량이 높았다. A batch experiment was conducted to evaluate the removal capacity of welsh onion(Allium fistulosum L.), shallot(Allium ascalonicum L.), garlic(Allium sativum L.) roots as an adsorbent for Ni and Pb in aqueous solution. One gram of the dried Allium root powder was reacted in 100ml of solution containing 10㎎ of each heavy metal and effects of metal concentration, pH, temperature, and size of adsorbent on the removal efficiency were evaluated. The results were as follows : The amount of adsorption of heavy metal ions were higher with the smaller particles size of adsorbent. Garlic root was high adsorption capacity of Pb, especially. The higher concentration of heavy metal solution was, the more amount of adsorption of heavy metals was. The adsorption ratio was differed from a kind of heavy metal. As the temperature increased, the amount of adsorption of Ni and Pb by shallot and welsh onion were decreased. The amount of adsorption of Ni was high under alkali conditions but the amount of adsorption of Pb was high under neutral and acidity condition.

허혈 조건부여가 심근기능의 회복에 미치는 영향 : 적출 심장모델을 이용한 실험연구 Isolated heart experimental study

백광제,김준식,한승백,전영진,이인성 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: Brief episode of coronary artery occlusion(i.e.,ischemic preconditioning) makes the heart more resistant to injury from a subsequent ischemic insult. Although a great deal of effort has been made in studying ischemic preconditioning, the underlying mechanism of ischemic preconditioning and its effect on hypothermic insult has not been elucidated. This study was performed to see whether ischemic preconditioning protects against the depression of cardiac contractility induced by hypothermic cardioplegic arrest/reperfusion. And recently, adenosine was known to have some correlation with the mechanism of preconditioning. If so, does this effect remain after the blockade of adenosine receptor by 8-phenyl theophyline? Method: Twenty-four Sprague-Dawley rat weighed 250-350g were used and divided into three groups. Rat hearts were removed rapidly, and each isolated heart paced with a rate of 180/min was perfused by modified Krebs-Hensleit buffer(KHB) solution on a Langendorff apparatus for an hour. After obtaining baseline data including left ventricular pressure(LVP), dp/dt, and coronary flow, cardiac arrest was induced by perfusion of 0℃ crystalloid cardioplegic(St Thomas) solution. After that, all hearts were stored in the same St Thomas solution at same temperature for 2 hours. In group Ⅰ(control group), the heart was reperfused by KHB solution. In group Ⅱ(preconditioning group), the heart was subjected to two 2-minute episode of global ischemia followed by 5 minute reperfusion with KHB solution(preconditioning) before cardiac arrest. In group Ⅲ(phenyl theophylline group), the heart was subjected to preconditioning procedure and 8-phenyl theophylline at 10μM in concentration was added to KHB solution at time of reperfusion. Observing parameter was obtained in each group at 10, 20, 40 and 60 minutes after starting reperfusion and compared statistically by use of one way ANOVA test(STASTICA, release 4.5). p-value less than 0.05 was considered significant. Results: Although depressed LVP, dp/dt, and coronary flow were seen in all groups during the reperfusion period, the preconditioned group showed more effective recovery of LVP than that of the control group, especially at 10, 20 and 40 minutes(p<.05). We failed to demonstrate the difference between the phenyl theophylline group and the control group(p=NS). Conclusion: These results suggest that ischemic preconditioning has protective effect on recovery state of hypothemic cardioplegic arrest/reperfusion. Its protective effect was limited during early reperfusion stage and was blocked by adenosine blocker.

체육교수 유형 및 지도 이론에 관한 연구

김광휘,김백중 대구대학교 인문과학연구소 2004 人文科學硏究 Vol.28 No.-

In this study, we wished to contribute in leadership ability elevation of sports leaders by investigating theory about professor type and coaching method synthetically by literature. Good physical education professor is teacher who is teacher who possess efficient professor facility and maximizes learner's accomplishment degree through class. Professor's efficiency can improve through training and quantitative test is available. Coaching is based on leader's experience and knowledge and do it so that player may establish target and reach. Therefore, coaching is according to student's skill and subject, and the method is also changed by them. Teacher should be tried to enlarge student's studying area by efficient physical education, and the process of the selection, performance and evaluation of ideal coaching method have to be repeated.