Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Ji Yeon Son,Yoon Jong Kang,Kyeong Seok Kim,Tae Hyung Kim,Sung Kwang Lim,Hyun Jung Lim,Tae Cheon Jeong,Dal Woong Choi,Kyu Hyuck Chung,Byung Mu Lee,Hyung Sik Kim 한국독성학회 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

유전성 대사질환에서의 동종조혈모세포이식 : 단일 기관에서의 경험 A single center experience

유건희,김흥렬,이지은,이호영,천정미,성기웅,구홍회,이문향,진동규,김종원,김대원,김형록 대한조혈모세포이식학회 2001 대한조혈모세포이식학회지 Vol.6 No.2

배경: 유전성 대사질환의 치료로서 효소 요법 및 유전자 치료가 제한적인 현실에서 동종 조혈모세포이식이 현재로선 가장 중요한 치료가 될 수 있다. 본 연구에서는 단일 기관에서 유전성 대사질환에 대해 동종 조혈모세포이식을 시행한 경험을 보고하고자 하였다. 방법: 삼성서울병원 유전성 대사질환 조혈모세포이식팀에서는 1999년 12월부터 2001년 3월까지 총 6례의 유전성 대사질환을 대상으로 동종 조혈모세포이식을 시행하였다. 대상 질환은 Hunter 증후군 3례, galactosialidosis 1례, 이염성 백질이영양증 1례, 부신백질이영양증 1례였으며 성별 분포는 남아가 5명, 여아가 1명이었으며 연령 분포는 2년 9개월에서 15년 9개월이었다. 5례는 HLA 일치 혈연간 골수이식이었으며 1례는 HLA 불일치 T 림프구 제거 말초혈액 조혈모세포이식이었다. 전처치는 모두 BuCy를 사용하였으며 이식편대숙주반응의 예방에는 cyclosporine을 사용하였다. 이식된 세포는 유핵세포가 4.81×10^(8)(2.40~7.01×10^(8))/kg이었으며 CD34+ 세포는 3.65×10^(6)(0.88~10.72×10^(6))/kg이었다. 결과: 이식 후 조혈기능의 회복은 모두 조기에 달성되었으며(ANC>500: 정중 9.5일, 범위 9~14일; PLT>50K: 정중 32일, 범위 23~34일) 이식과 관련된 합병증은 Gr I의 aGVHD 3례, 국한성의 cGVHD 1례, 경증의 간정맥 폐쇄성 질환이 1례이었다. 모든 환자에서 직간접적으로 이식 후 효소의 생산이 증가함이 확인되었으며 임상적인 호전을 보인 경우가 4례, 질병 진행이 중단된 경우가 1례, 질병이 진행된 경우가 1례이었다. 질병 진행이 중단되었던 1례는 면역억제제 투여 중 수두 감염에 의한 폐출혈로 사망하였다. 결론: 유전성 대사질환에서 동종 조혈모세포이식이 가장 중요한 치료법으로 사용될 수 있고 중추신경계 증상이 나타나기 전, 가능한 조기에 조혈모세포이식을 시행하는 것이 바람직할 것이며 적극적인 지지 요법이 필요하다. 향후 더 많은 임상 경험이 필요하리라 사료된다. Background: Allogeneic hematopoietic stem cell transplantation (HSCT) may be the most important treatment modality to cure a number of genetic metabolic disorders because of the limitation of enzyme replacement or gene therapy. In this study, we report our single center experience about HSCT in several genetic metabolic disorders. Methods: We performed 6 cases of HSCT for genetic metabolic disorders from December 1999 to March 2001. Patients' diagnoses were Hunter syndrome (3), galactosialidosis (1), metachromatic leukodystrophy (1), and adrenoleukodystrophy (1). Stem cell sources were bone marrow from HLA matched sibling donors in 5 patients and mother's peripheral blood stem cells in one patient who did not have HLA matched donors. Busulfan and cyclophosphamide for conditioning, and cyclosporine for the prevention of graft versus host disease were used in all patients. Transplanted total nucleated cell counts were median 4.81×10^(8)(2.40~7.01×10^(8))/kg , and CD34+ cells 3.65×10^(6)(0.88~10.72×10^(6))/kg. Results: All patients achieved early hematologic recovery (median 9.5 days, range 9~14 days for ANC>500/μL; median 32 days, range 23~34 days for PLT>50,000/μL). Transplant-related complications were 3 cases of grade 1 acute GVHD, a case of limited chronic GVHD, and a case of mild hepatic veno-occlusive disease. Enzyme levels were normalized in 4 patients evaluated and there were indirect evidences of enzyme production in the other 2 patients after HSCT. Four of the 6 patients showed symptomatic improvement, 1 patient (galactosialidosis) experienced disease stabilization without progression before he eventually died due to pulmonary hemorrhage, and the other 1 patient deteriorated progressively even after HSCT. Conclusions: Allogeneic HSCT can be done as the only curative treatment in a number of genetic metabolic disorders. It seems desirable to perform HSCT as early as possible before the onset of central nervous system symptoms. More experience and long term follow up is needed to evaluate the efficacy and to monitor the long term transplant-related complications.

Evaluation of Renal Toxicity by Combination Exposure to Melamine and Cyanuric Acid in Male Sprague-Dawley Rats

Son, Ji Yeon,Kang, Yoon Jong,Kim, Kyeong Seok,Kim, Tae Hyung,Lim, Sung Kwang,Lim, Hyun Jung,Jeong, Tae Cheon,Choi, Dal Woong,Chung, Kyu Hyuck,Lee, Byung Mu,Kim, Hyung Sik Korean Society of ToxicologyKorea Environmental Mu 2014 Toxicological Research Vol.30 No.2

Melamine-induced nephrotoxicity is closely associated with crystal formation in the kidney caused by combined exposure to melamine (Mel) and cyanuric acid (CA). However, there are few dosage-finding studies for toxicological evaluation of chronic co-exposure to Mel and CA. The objective of this study was to investigate the possible mechanism by which a Mel and CA mixture lead to renal toxicity in rats. Mel and CA were co-administered to rats via oral gavage for 50 days. Nephrotoxicity was determined by measuring blood urea nitrogen (BUN) and serum creatinine (sCr) levels. Relative kidney weights were significantly increased in rats after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg) mixtures. BUN and sCr levels were significantly increased after Mel and CA co-exposure. Taken together, significant increase in KIM-1, NGAL, and calbindin levels were observed in the urine of rats exposed to Mel+CA (63/6.3 or 630/6.3 mg/kg) compared with the corresponding control group. Histological analysis revealed epithelial degeneration and necrotic cell death in the proximal tubules of the kidney after co-exposure to Mel+CA (63/6.3 or 630/6.3 mg/kg). Our data suggest that Mel-mediated renal toxicity may be influenced by CA concentrations in Mel-contaminated milk or foods.

골프 드라이버 스윙 임팩트 시 팔의 추가 당김이 클럽헤드 속도에 미치는 영향

김종숙,서국웅 釜山大學校 附設 體育科學硏究所 2005 體育科學硏究所 論文集 Vol.21 No.1

The purpose of this study was to find out variables to increase the golf driver distance through simulation analysis of the double pendulum model on computer. Also, it was performed in order to examine the effects of additional pull of the arm on the velocity of the clubhead in moment of the golf driver swing and provide the scientific basis for improving the skill of swing. The conclusions are as follows :........

골프 경기력 향상을 위한 골프공의 선택에 관한 연구

김종숙,서국웅 釜山大學校 附設 體育科學硏究所 2007 體育科學硏究所 論文集 Vol.23 No.1

This research was carried out to find data to get better result in golf game through analyzing the distance and dispersion by structure of golf ball among phenomenon on during golf ball is flying. But the difference that analysis result is worth keeping in mind did not appear. According to characteristics of that is golf game, microscopic difference decides game result. Therefore, supposing the golfers select ball via follwing item, may satisfy in game result......

양성 및 음성 정신분열병 아형에서 사고의 정신병리적 특성에 관한 비교연구

김종숙,함웅,홍광화 대한신경정신의학회 1994 신경정신의학 Vol.33 No.3

The authors have studied the content and severity of thought disorder between the subjects of the positive and negative subtypes of schizophrenia. It's hypothetical ground is that the positive and negative subtypes of schizophrenics might bear highly significant differences in the pathogenesis, pathophysiology, psychopathology, also therapeutic and prognostic terms. In that regards, the author reported the neuropsychological significance in differentiating the two subtypes from each other, such that the negative subtype showed the worse function in the short-term recall against the auditory stimuli than the positive. To comparatively analyze the content and severity of thought disorder between the two subtypes, the authors utilized the Thought Discorder Index(TDI) scores and Holt's scale of the primary process thinking content. The results were as follows: 1) The total score of TDI as a whole, showed somewhat more thought disorder in the positive subtype subjects than the negative, though this failed to reach the statistical significance. In 0.75 level of TDI, the positive subtype subjects showed statistically significantly worse thought disorder than the negative. 2) In the comparative study of the primary process thinking content, the positive subtype subjects showed degree in the aggressive content but lower degree in the anxiety content than the negative subtype subjects with statistical significance. 3) In the correlation study, 0.5 level of TDI correlated with the libidinal content of the primary process with blunted affect and the aggressive content with lack of spontaneity and flow of conversation. The above result might suggest that the positive subtype of schizophrenia demonstrated more severe thought disorder, if we regard that responses scored at the 0.75 level of TDI represent clear thought disturbances. The negative subtype of schizophrenia tends to possess higher anxious and libidinal content of the primary process thinking.

삼풍백화점 붕괴 대참사의 법의치과학적 개인식별 : Using the Forensic Odontological Studies

김종열,이영석,윤창륙,허웅,최종훈,김재홍 內務部 國立科學搜査硏究所 1996 國立 科學 搜査 硏究所 年報 Vol.28 No.-

원형질체 융합에 의한 체세포 잡종의 형질 발현에 관한 연구 : Ⅰ. Nicotiana tabacum과 N. sylvestris의 원형질체 융합에 의한 체세포 잡종의 생성 및 형질 발현 Ⅰ. Somatic Hybrids by Protoplast Fusion between Nicotiana tabacum and N. sylvestris

김준철,원종락,이광웅 慶尙大學校 기초과학연구소 1987 基礎科學硏究所報 Vol.3 No.-

담배재배종인 Nicotina tabacum cv.Nc 2326과 야생종인 N.sylvestris 간의 원형질체 융합에 의한 체세포 잡종을 생성하고 이들 잡종의 유전 및 형태적 특성을 모식물체와 비교한 결과는 다음과 같다. 1. 체세포 잡종식물의 엽조직에서의 peroxidase 밴드패턴을 부분적으로 함께 나타내고 있었다. 2. 꽃의 색깔과 길이, 초장은 모식물체의 중간형이었고 잎배열은 N.tabacum에 엽맥의 색깔은 N.sylvestris에 가까웠으며 그밖의 형질은 일정한 경향이 없었다. 3. 체세포 잡종식물은 개화는 되었으나 종자의 결실은 되지 않았다. Somatic hybrids were produced in the callus derived from the fused cells between Nicotiana tabacum cv. NC 2326 and N. sylvestris. In biochemical analysis of the somatic hybrids the peroxidase isozyme patterns in the leaf partly showed the profiles of both parents. In the hybrids the flower colour and length, and plant height were the intermediate form of both parents. In leaf arrangement the hybrids were similar to N. tabacum and in colour of mid vein they were similar to N. sylvestris. The other phenotypes had no tendency as compared to their parents. The hybrid plants had flowers but could not set seeds.

Clopidogrel에 의해 발생된 전신 염증 반응 증후군 1례

김민환,김종훈,박경일,박혜연,황철웅,김의석,도준형,남궁준,이성윤,이원로 白中央醫療院 2005 仁濟醫學 Vol.26 No.1

Clopidogrel bisulfate, a widely used inhibitor of platelet aggregation, is considered at least as safe as aspirin. We describe a 61 year old male patient who developed a systemic inflammatory response syndrome consisting of high fever, rash, chills, impaired liver function, and mild leukopenia after receiving clopidogrel after coronary angiography and stent implantation. The reaction resolved promptly after withdrawal of the drug, thus making the diagnosis of a clopidogrel induced reaction highly probable.

만성 주정중독자의 뇌전산화 단층촬영 소견

김형동,김종길,오민웅 大韓神經精神醫學會 1984 신경정신의학 Vol.23 No.2

Authors studied the degree of atrophy of alcoholics by measuring ventricle size, cortical sulci size and bifrontal cerebroventricular index. Subjects were consisted of 34 chronic alcoholics and the control group of 25 non-alcoholic neurotics, who were treated at the Department of Neuropsychiatry at Maryknoll Hospital from Mar. 1, 1982 to Aug. 8, 1983. The results were as follows: 1. Cerebral atrophy was found among 24 cases(70.6%) of chronic alcoholics. However, only 1 case of cerebral atrophy was found among the control group. 2. For chronic alcoholics sulci was 6.64±1.48mm, ventricle size was 17.14±2.37mm and the bifrontal cerebroventricular index was 35.6±5.3%, which suggested statistically significant cerebral atrophy, as compared to the control group(p<0.05).