익상구개와에 발생한 악성 섬유조직구종 1예

홍정표,김인섭,김문석,윤주헌 대한이비인후과학회 1999 대한이비인후과학회지 두경부외과학 Vol.42 No.9

음향방출 및 가속도 신호를 이용한 지하매설 상수도배관의 누수지점 탐지연구

이영섭,윤동진,정중채 한국비파괴검사학회 2003 한국비파괴검사학회지 Vol.23 No.3

지하매설 상수도 배관의 누수는 사회적, 환경적 그리고 경제적 문제를 야기시킨다. 이러한 누수에 대한 적절한 대응책의 하나로서 배관의 누수지점에 대한 탐지 및 수리가 해당될 것이다. 누수음은 배관의 누수지점의 식별에 좋은 탐지원이다. 비록 청음봉, 층음기 또는 지상마이크로폰과 같은 누수음으로 누수지점을 탐지하는 몇가지의 방법이 있어 왔지만, 그들은 그렇게 효율적인 도구들은 아니었다. 본 논문에서는, 좀 더 쉽고 효율적인 방법을 제공할수도 있는 음향방출센서와 가속도계를 사용한 누수위치탐지를 다룬다. 누수지점 탐지를 위해 센서로 부터의 입력신호에 대한 필터링, 신호처리 및 알고리듬을 기술하였다. 120 미터 길이의 배관이 실험을 위해 설치가 되었고, 실험결과들은 음향방출센서와 가속도계가 누수지점의 정밀탐지에 이용될 수 있음을 보여 주었다. 누수지점의 탐지에 극히 중요한 지하매설배관 속의 유체의 음파 전파속도에 관한 이론적 해석 또한 기술되었다. Leaks in underground pipelines can cause social, environmental and economical problems. One of relevant countermeasures against leaks is to find and repair of leak points of the pipes. Leak noise is a good source to identify the location of leak points of the pipelines. Although there have been several methods to detect the leak location with leak noise, such as listening rods, hydrophones or ground microphones, they have not been so efficient tools. In this paper, acoustic emission(AE) sensors and accelermeters are used to detect leak locations which could provide an easier and more efficient method. Filtering, signal processing and algorithm of raw input data from sensors for the detection of leak location are described. A 120 m-long pipeline system for experiment is installed and the results with the system show that the algorithm with the AE sensors and accelerometers offers accurate pinpointing of leaks. Theoretical analysis of sound wave propagation speed of water in underground pipes, which is critically important in leak locating, is also described.

출아효모에서 TAR 클론닝법을 이용한 고등동물의 게놈으로부터 특정 염색체 부위의 분리

박정은,윤영호,이윤주,김광섭,윤세련,안태진,임선희,선우양일 동아대학교 기초과학연구소 2003 基礎科學硏究論文集 Vol.20 No.1

복잡한 게놈 분석에 용이하도록 효모의 인공 염색체(YAC) 클론닝 시스템은 발달되어왔다. YAC은 박테리아의 인공 염색체(BAC)보다 더 큰 단편을 클론닝할 수 있고, 또한 클론된 단편을 쉽게 변형시킬 수 있다. 형질전환과 연계된 재조합법(Transformation-Associated Recombination; TAR)은 게놈 라이브러리를 만들지 않고 직접 게놈 DNA로부터 분리하고자 하는 유전자나 특정 염색체 부분을 클론닝 할 수 있는 방법이다. 이 방법은 spheroplast transformation을 수행하는 동안, 목적으로 하는 유전자의 5' 그리고 3' 염기 배열(hooks)을 지닌 TAR 벡터와 게놈 DNA 사이에서 일어나는 상동성 재조합에 의해 이루어진다. 효모 내의 in vivo 재조합을 이용한 TAR 클론닝법은 복잡한 게놈으로부터 목적의 염색체 부분을 원형 YAC의 형태로서 선택적으로 분리할 수 있다. 그러므로 TAR 클론닝 법은 특정 염색체로부터 YACs을 만드는데 매우 유용하여, 전체 게놈으로부터 특이적 유전자나 유전자의 family를 분리하는데 효과적인 방법으로 사료된다. Yeast artificial chromosome (YAC) cloning systems have advanced the analysis of complex genomes considerably. They permit the cloning of larger fragments than do bacterial artificial chromosome systems, and the cloned material is more easily modified. Transformation-associated recombination (TAR) is a cloning technique that allows specific chromosomal regions or genes to be isolated directly from genomic DNA without prior construction of a genomic library. This technique involves homologous recombination during spheroplast transformation between genomic DNA and a TAR vector that has 5' and 3' gene targeting sequences (hooks). Using in vivo recombination in yeast, TAR cloning selectively isolates, as circular YACs, desired chromosome segments or entire genes from complex genomes. We propose that TAR cloning can provide an efficient means for generating YACs from specific chromosomes and that TAR cloning may be useful for isolating families of genes and specific genes from total genome DNA.

Urapidil, Labetalol의 투여가 기관내 삽관시 심혈관계에 미치는 영향

신용섭,윤석화,손수창,이원형,이정은,황원재,김만수,김영주,김혜자,최세진 충남대학교 의과대학 지역사회의학연구소 1994 충남의대잡지 Vol.21 No.2

We have examined the comparative efficacy of small doses of intravenous urapidil and labetalol in blunting hemodynamic response to endotracheal intubation and surgical incision in 30 patients without cardiovascular diseases. After intravenous urapidil 0.2 mg/kg or labetalol 0.2 mg/kg anesthesia was induced with thiopental 5mg/kg. Endotracheal intubation was facilitated by vecuronium 0.15 mg/kg with priming principle and anesthesia was maintained with enflurane and nitrous oxide in oxygen. Systolic, diastolic and mean arterial pressure and heart rate were measured before administration of the drugs, 5 minute after administration, just prior to endotracheal intubation and 1, 3, 5, 10 minute after intubatin. Also the peak blood pressures and heart rate within 10 minutes after surgical incision were measured. Endotracheal intubation and surgical stimulation were associated with significant increases in blood pressures and heart rate in both urapidil and labetalol group. Comparison of the changes in systolic, diastolic, and mean artrial pressures and heart rate between urapidil and labetalol group showed no significant difference except peak systolic pressure after surgical incision. It is concluded that the pressor response to endotracheal intubation and surgical stimulation are not influenced significantly by urapidil 0.2 mg/kg or labetalol 0.2 mg/kg. However, urapidil and labetalol preloading may be similarly effective in the blunting of the increases in blood pressures with larger doses of the durgs during anesthetic induction.

지하매설 상수도배관의 누수지점 탐지를 위한 탄성파 검출연구

이영섭,윤동진,정중채,이승석 한국비파괴검사학회 2003 학술대회 논문집 Vol.- No.1

Leaks in underground pipelines can cause social, environmental and economical problems. One of a good contermeasures of leaks is to find and repair of leak points of pipes. Leak noise is a good source to identify thc location of leak points of pipelines. Although there have been several methods to detect thc leak location with leak noise, such as listening rods, hydrophones or ground microphones, they were not so efficient tools. In this paper, two accelermeters are used to detect leak locations which could provide an easier and effcient method. The filtering, signal processing and algorithm is described for the detection of leak location. A 120m-long pipeline system for experiment is installed and the results with the system show that the algorithm with the two accelerometers gives very accurate pinpointing of leaks. Theoretical analysis of sound wave propagation speed in underground pipes is also described.

'선도 추격'에서 '동반 추격'으로의 전환을 위한 서비스 산업의 역할과 전략 : 한일 생산성 비교의 시사

이근,정무섭,김윤지 韓國東北亞經濟學會 2008 동북아경제연구 Vol.20 No.2

The Korean economy has grown very fast over the last decades, but it was an uneven catch-up led by a big business and manufacturing. Now it is time for co-catchup led by both manufacturing and services. This paper analyzes the weight and productivity of the service sector in Korea and suggests for policies for its improvement in productivity. The service sector in Korea is featured by low and declining productivity (both TFP and labor productivity), and a big trade deficits, which is in contrast to the trade surplus in manufacturing. This performance is worse, compared with the situation in Japan. For example, the ratio of labor productivity in service to that in manufacturing is only 14%, compared to 46% in Japan. Causes for the low performance in service include too much regulation and lack of competition due to the closed market. Thus, measures to boost up productivity in service include deregulation and market opening, which would open up the opportunity for learning from foreign companies. Industrial policy should also change from input-oriented one to network-oriented one emphasizing the unity of commodities and services.

UV-TiO₂광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정해광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경공해연구소 1998 環境公害硏究 Vol.14 No.-

UV-TiO_(2) 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254 ㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_(2)는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7× 10^(7) cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0× 10^(2) cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 3.4× 10 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 석영관에 박막증착된 경우에는 7.6× 10^(2) cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입후 11분 동안 자외선을 조사시킨 경우에는 1.3× 10^(2) cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_(2)를 첨가하였을 경우에는 1× 100 cells/㎖으로, 자외선 조사와 함께 TiO_(2)가 박막증착된 경우에는 7.9× 10 cells/㎖을 나타내었다. 결국 UV-TiO_(2) 반응기에 사용되는 TiO_(2)가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_(2) photocatalytic system on the Escherichia coli DH5-慣 and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 nm was set on the center of pyrex round glass tube. Two types of TiO_(2), one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7 * 10^(5) cells/??of E. coli for 11 min, the living cell number decreased to 4.0 * 1.0^(0) cells/?? The effect of UV system together with slurried TiO_(2) was less than 1 cells/?? whereas that of UV-coated TiO_(2) system decreased to 7.1 * 10^(3) cells/?? To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7 * 10^(5) cells/??were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3 x 10^(2) cells/?? In the same condition except the addition of slurried TiO_(2), the living cells were 1 * 10^(2) cells/?? However, more cells could be killed by the system which consists of UV, coated TiO_(2), and air bubble (7.9 * 10^(1) cells/??. From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_(2) system.

peat moss를 이용한 폐수중의 중금속 제거

이경화,김정섭,유현철,최성우,윤철종,우성훈,박승조 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

Adsorption equilibrium of heavy metals ion on the peat moss was confirmed Frundlich isotherm Removal efficiency of heavy metals ion was increased from pH 2 to 10 and heavy metal removal efficiency was increased in the sequence of Cu(Ⅱ) > Pb(Ⅱ) > Cd(Ⅱ) > Mn(Ⅱ) on the PM(Peat Moss). The PM is likely to be more effective adsorption than the PAC to removal of metal ions in the solution, since PM's weakly acidic functional groups which is not founded on the PAC, in addition to its high surface area and porosity. But, the PM is not separated from the solution water by sedimentation process. So, a liquid and solid seperation between the PM and the solution water could be considered for the PM's field application. But peat moss was low cost adsorbent. Thus author propose to adopt PM as a adsorbent for heavy metal removal.

UV-TiO_2 광촉매 반응기를 이용한 미생물의 살균효과

김시욱,이정섭,정혜광,박열,윤성명,유진철,이범규,이인화,박진열 조선대학교 환경연구소 1998 環境硏究 Vol.14 No.1

UV-TiO_2 반응기를 이용하여 Escherichia coli와 Aspergillus oryzae var. oryzae의 살균효과를 측정하였다. 254㎚에서 최대 14 watt의 자외선 방출량을 내는 램프를 원형 Pyrex유리관 중앙에 설치하였고 TiO_2는 석영관에 박막증착(Thin Film Coating)된 형태와 슬러리 형태로 나누어 회분식으로 살균정도를 측정하였다. E. coli에 대한 살균력은 1.7×10^7 cells/㎖에 대해 5분간 자외선 조사를 하였을 경우 2.0×10^2 cells/㎖으로 감소하였고, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 3.4×10 cells/㎖으로, 자외선 조사와 함께 TiO_2가 석영관에 박막증착된 경우에는 7.6×10^2 cells/㎖으로 감소하였다. 한편 위와 같은 조건에 유리관 하부에서 기포를 주입수 11분 동안 자외선을 조사시킨 경우에는 1.3×10^2 cells/㎖으로, 자외선 조사와 함께 슬러리 형태의 TiO_2를 첨가하였을 경우에는 1×102 cells/㎖으로, 자외선 조사와 함께 TiO_2가 박막증착된 경우에는 7.9×10 cells/㎖을 나타내었다. 결국 UV-TiO_2 반응기에 사용되는 TiO_2가 슬러리 형태일 때 최대 살균효과를 나타내었으나 기포가 첨가되었을때는 오히려 살균에 장애를 받는 것으로 나타난 반면 석영관에 박막증착된 경우에는 기포가 첨가되는 것이 살균에 효과적인 것으로 관찰되었다. The killing effect of UV-TiO_2 photocatalytic system on the Escherichia coli DH5-α and Aspergillus oryzae var. oryzae (KCTC 6095) was studied. The UV lamp which emits maximum 14 watts at 254 ㎚ was set on the center of pyrex round glass tube. Two types of TiO_2, one of which is slurry and another which is thin film coated form, were used to determine the killing effect. When UV was irradiated to 1.7×10^5 cells/㎖ of E. coli for 11 min, the living cell number decreased to 4.0×10^0 cells/㎖. The effect of UV system together with slurried TiO_2 was less than 1 cells/㎖, whereas that of UV-coated TiO_2 system decreased to 7.1×10^3 cells/㎖. To study the effect of bubble on the killing of microorganisms, air was bubbled to the bottom of glass tube. When 1.7×10^5 cells/㎖ were exposed to UV for 11 min in combination with air bubble, the living cell number decreased to 1.3×10^2 cells/㎖. In the same condition except the addition of slurried TiO_2, the living cells were 1×10^2 cells/㎖. However, more cells could be killed by the system which consists of UV, coated TiO_2, and air bubble (7.9×10^1 cells/㎖). From these results, it was found that UV-slurried system is the most effective one, but its killing effect is not stimulated by air bubble. However, bubbling was very effective in the UV-coated TiO_2 system.

석탄회 및 석고첨가가 퇴비화에 미치는 영향

유현철,김정섭,곽명화,윤철종,이희인,박승조 동아대학교 환경문제연구소 2001 硏究報告 Vol.24 No.1

Problems of wastes that pigs produce are increasing rapidly in the hog raising industry nowadays. consequently, the generation volume of excreta also is increasing but most of that is not disposed appropriately and exposed in eco-system therefore the disposal of stock excreta is becoming more and more serious. considering the physical and chemical characteristics of livestock excreta, the pollution loading rate - BOD, COD etc. - is very high. as alternative plan, that is, as one method kind of composisting, the utilization of the soil reformation agent will become one solution in managing the upper problems. also, the flyash of 3.7 million tons are producing every year and about 32.2% of that, 1.18 million ton is recycled as concrete, cement, cementassistance, road etc. consequently, the discharged flyash is not abandoned and a plan of recycling is needed acutely. gypsum of 4.16 million tons in 1998 presently was produced and about 57.2% of that, 2.38 million tons was recycled as cement, construction materials. the present stock is 25 million tons. therefore, in this study, because organic materials can be obtained in pig excreta and calciums can be obtained in gypsum, the small amount of elements can be acquired in flyash for the soil reformation agents, mixing the three materials - pig excreta, gypsum, flyash - adequately, investigated technique of making the soil reformation agents and described below in comparrision with the changes of temperature, PH TOC, T-N, TOC/T-N ratio etc.