제주산 보통온주의 저온저장

梁相互,高正殷,金聖學,高正三 濟州大學校 亞熱帶農業硏究所 1994 亞熱帶農業硏究 Vol.11 No.-

제주산 청도온주의 저온저장조건을 검토하였다. 저장기간 중 3월 하순까지는 과피수분, 가용성고형물, 총당, 비타민 C, 비중의 변화가 매우 적었으며, 부패율이 20%수준으로서 비교적 신선도를 유지한 상태에서 저장이 가능한 것으로 보였다, 그러나 그 이후에는 온도가 낮을수록 냉해증상이 심하게 일어나 부패과의 발생이 많아지면서 저장이 어려웠다. 또한, 중량감소, 경도의 저하, 산함량의 저하는 저장기간동안 계속하여 서서히 일어났다. 온주밀감의 경우 저온에서 장기간 저장이 어려움을 알 수 있어서 저장용 감귤의 특성을 고려하여 저장조건을 설정할 필요가 있었다. The conditions of cold stroage of Citrus aoshima unshiu produced in Cheju were investigated. The changes of peel moisture content, soluble solids, total sugar, vitamin C and density were slightly occured, and decay ratio was below 20% on keeping freshness relatively till late of March during cold storage. After that, decayed citrus fruits were increased from cold injury with lower temperature. The loss of fruit weight, decrease of fruit hardness, and decrease of acid content were occured gradually during cold stroage. Because of the difficulty of long term storage for Citrus unshiu, the conditions and periods of cold storage would be determined after considering the physicochemical properties of fruits every year.

두피 전체의 발모광을 보이는 정신분열병 1례

고정인,신성훈,이상열 圓光大學校 醫科大學 神經精神科學敎室 1998 圓光精神醫學 Vol.14 No.1

Trichotillomania is characterized by recurrent pulling of one's hair, resulting in significant hair loss and involves many hours each day of pulling hair or thinking about pulling hair. Patients pulled hair from a variety of sites, including scalp, eyelashes, eyebrows, pubic region, face, and body, but the highest incidence and severity involved scalp hair. This symptom asries as a part of symptom complexes in a variety of psychiatric disorders such as obsessive compulsive disorder, depression, mental retardation, eating disorder and schizophrenic disorder. The authors experienced a case of trichotillomania in a 42 year old schizophrenic patient whose total hair in scalp was pull out. Several literatures were reviewed and psychiatric aspect of trichotillomania were discussed. For the treatment of this patient, haloperidol and supportive psychotherapy had been tried and been proven effective.

연령에 따른 흰쥐 망막의 기저복합체의 변화에 대한 미세구조적 연구

고정식 순천향대학교 1989 논문집 Vol.12 No.1

본 실험은 연령에 따른 흰쥐 망막 기저복합체의 미세구조적 변화를 관찰하기 위하여 시행하였다. 실험동물로는 생후 3개월, 12개월 및 30개월된 Fisher 344계 흰쥐를 각군에 4마리씩 사용하였다. 각 동물은 4% chloral hydrate 마취하에 안구를 적출하였다. 적출된 안구에서 초자체를 제거한 후, 망막후부중 일정부위의 망막을 절취하였다. 절취된 조직은 2.5% glutaraldehyde-1.5% paraformaldehyde고정액(0.1 M phosphate buffer, pH7.3) 에 1차 고정한 후, 1% osmium tetroxide 액(0.1 M phosphate buffer, pH 7.3) 에 2차 고정하였다. 고정된 조직은 탈수과정을 거쳐 araldite에 포매한 후 LKB-V ultratome으로 60-70nm 두께의 얇은 절편을 만들었다. 각 절편은 uranyl acetate와 lead citrate로 대조염색을 시행한 후 JEM 100CXⅡ 전자현미경으로 관찰하였다. 기저복합체를 구성하는 5 층의 두께 측정은 각군당 50 매씩의 사진에서 비스듬히 절단된 기저복합체로 인한 오차를 줄이기 위해, 각 사진에서 얇은 부분을 측정하였다. 결과는 다음과 같다. 1. 망막 기저복합체의 두께는 어린 흰쥐의 것은 474nm, 젊은 흰쥐의 것은 525nm 및 늙은 흰쥐의 것은 704nm로서 연령증가에 따라 현저히 두꺼워졌다. 2. 맥락막의 모세혈관 기저막의 두께는 어린 흰쥐의 것이 121nmduTsmsep 비하여 젊은 흰쥐와 늙은 흰쥐의 것은 각각 66nm와 54nm로서 연령이 증가함에 따라 얇아졌다. 3. 외측 교원섬유층의 두께는 어린 흰쥐의 것은 132nm, 젊은 흰쥐의 것은 139nm 및 늙은 흰쥐의 것은 232nm로서 연령이 증가함에 따라 두꺼워졌다. 4. 탄력섬유층의 두께는 어린 흰쥐의 것이 91nm였고, 젊은 흰쥐와 늙은 흰쥐의 것은 각각 147nm와 142nm로서 연령이 증가함에 따라 두꺼워졌다. 5. 내측 교원섬유층의 두께는 어린 흰쥐의 것이 87nm였고, 젊은 흰쥐와 늙은 흰쥐의 것은 각각 106nm 와 119nm로서 연령이 증가함에 따라 두꺼워졌다. 6. 색소상피 기저막의 두께는 어린 흰쥐의 것이 43nm였고, 젊은 흰쥐와 늙은 흰쥐의 것은 각각 67nm 와 157nm로서 현저히 두꺼워졌다. 7. 늙은 흰쥐의 경우, 색소상피 세포와 기저막 사이에 미세한 섬유상 또는 과립상물질<기저침전물>이 침전되어 있는 경우가 자주 관찰되었다. Morphological difference of the Bruch's membrane at different age groups have been studied in young (three months-old), adult(twelve months-old) and old(thirty months-old) Fisher strain 344 rats. Pieces of the tissues taken from the posterior regions of the retina were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde (0.1M Millonig's phosphate buffer, pH7.3) and 1% osmium tetroxide(0.1M Millonig's phosphate buffer, pH7.3), embedded in araldite mixture. The ultrathin sections contrasted with uranyl acetate and lead citrate were observed under a JEM 100 CX II electron microscope. The mean thickness of each of the five zones of the Bruch's membrane were determined by measuring the thinnest portion of Bruch's membrane, and by taking the average of 50 readings from electron micrographs at different ages. Observed results were as follows: 1. The thickness of the Bruch's membrane is increased during aging: 474 nm in young rats, 525 nm in the adult, and 704 nm in the old. 2. The thickness of the basal lamina of the choriocapillary endothelium is decreased during againg: 121 nm in young rats, 66 nm in the adult, and 54 nm in the old. 3. The thickness of the outer collagenous zone is increased during aging: 132 nm in young rats, 139 nm in the adult, and 232 nm in the old. 4. The thickness of the elastic zone is increased during aging: 91 nm in young rats, 147 nm in the adult, and 142 nm in the old. 5. The thickness of the inner collagenous zone is increased during aging: 87 nm in young rats, 106 nm in the adult, and 119 nm in the old. 6. The thickness of the basal lamina of the pigment epithelium is increased during aging: 43 nm in young rats, 67 nm in the adult, and 157 nm in the old. 7. Accumulaton of finely filamentous or granule-like materials(basal deposit) between basal foldings of pigment epithelium and above its thickened basal lamina is more frequently observed in old rats.

BCG를 대량 투여 받은 생쥐 가슴샘 겉질의 미세구조

고정식,안의태,박경호,우성임,한영복,경홍기 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.3

상악골 전방 견인이 상악골체의 변위에 미치는 영향

고정석,김종철 대한치과교정학회 1995 대한치과교정학회지 Vol.25 No.5

골격성 부정교합에 대한 악정형 치료시, 바람직한 악정형 효과를 얻기 위해서는 악정형 장치의 역학 현상에 대한 분석이 필요하다. 상악의 저성장에 기인한 골격성 Ⅲ급 부정교합의 치료를 위한 상악골의 전방견인시 바람직한 견인력 방향과 견인 위치를 알아보기 위해, 성인 두개골을 대상으로 3차원 유한 요소 모델을 제작하여, 제1소구치와 제1대구치에서 각각 교합평면에 평행 또는 20˚하방으로 500g의 전방 견인력을 주어 상악골체의 각 부분에서 응력과 변위를 분석하여 다음과 같은 결과를 얻었다. 1. 모든 경우 상악골이 반시계 방향으로 회전하는 양상을 보였다. 2. 견인력 방향에 따른 상악골의 회전은, 20˚하방으로 견인하는 경우가 평행하게 견인하는 경우보다 더 적었다. 3. 견위 위치에 따른 상악골의 회전은, 평행하게 견인시에는 견위 위치가 제1대구치일 때가, 20˚하방으로 견인시에느 제1소구치일 때가 더 적었다. 이상의 결과 견인력 작용선이 상악골체의 저항 중심점에 가장 근접하여 반시계 방향으로의 변위가 가장 적게 일어난 제1소구치에서 20˚하방으로 견인한 경우가 바람직한 견인력 방향과 견인 위치로 판단되었다. In the orthopedic therapy, the biomechanical analysis of the appliance is necessary to get a desirable orthopedic effect. The purpose of this study was to investigate the desirable direction and application position of the protraction force. The protraction force of 500g was applied to the first premolar or to the first molar. The direction of force application was paralell or 20˚downward to the occlusal plane respectively. The stress distribution and the displacement within the maxilla was analyzed by a 3-dimensional finite element method. The findings obtained were as follows : 1. Protraction forces caused a counterclockwise rotation of the maxilla. 2. The degree of maxillary rotation was less when the force was applied 20˚downward direction to the occlusal plane than when applied to the parallel direction. 3. The degree of rotation of maxilla was greater when the parallel force was applied to the 1st premolar than when applied to the first molar, whereas it was greater when force is applied 20˚downward than at the first premolar. In conclusion, the 20˚downward protraction from the first premolar induced the least counterclockwise rotation of the maxilla and was thought as the desirable direction and application position of the protraction force.

집토끼의 갑상선내 Parafollicular Cells에 대한 전자현미경적 연구

고정식,양남길 순천향대학교 1981 논문집 Vol.4 No.2

In order to study the fine structure of the parafollicular cells in the rabbit thyroid gland, small b1ocks of the thyroid tissue were fixed in the mixture of 2% paraformaldehyde-2.5 % glutara1dehyde (phosphate buffer, pH 7.4) and post-fixed in 1 % osmium tetroxide(phosphate buffer, pH 7.4). The tissue were embedded in Epon 812, ultrathin-sectioned with the LKB-Ⅲ ultramicrotome. The sections contrasted with uranyl acetate and lead citrate were observed with a JEM 100B electron microscope. Observed results were as follow: 1. Of the rabbit parafollicular cells. the cells with electron-lucent cytoplasm (light cell) and with electron-dense cytoplasm (dark cell) can be distinguished. 2. The parafollicular cells of the rabbit were observed in the intra-. para-and inter-follicular positions. Inter-follicular cells occur as groups of a few cello. None of intra-follicular cells have contact with the follicular lumen. 3. The parafollicular cells have oval or round indented nuclei. They contain moderately developed cell organelles. such as Golgi complex, granular doplasmic reticulum, ribosomes, etc. 4. The membrane-bound granules are spherical with a diameter of 150∼380nm. The electron densities of the core texture are variable from the coarse granule with lower density to the homogeneous granule with higher dentsity.

연령이 증가함에 따른 흰쥐 망막의 막대세포, 원뿔세포 및 바깥얼기층의 미세구조 변화

고정식,한종원,양남길,안의태 대한해부학회 1992 Anatomy & Cell Biology Vol.25 No.3

과도 절삭에서의 절삭 공정 시뮬레이션

고정훈,조동우,윤원수,김주한 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

In most of the existing mechanistic models, the cutting process simulation is often restricted to a single path machining operation under a fixed cutting condition. Complex cutting processes such as die or mold manufacturing, however, are performed under two- or three-dimensional multiple tool paths. Since the tool paths in CNC machining are composed of line and arc segments, transient cuts are frequently occured due to the multiple paths. Even in steady cuts, the width of cut is varied with each segment. In this regard, this paper deals with the development of process simulation system for transient cuts, where continuously changing cutting configuration is computed, and then the cutting forces are predicted.

교흔으로 인해 발생한 Eikenella corrodens 간농양 1예

고근준,이은재,최형섭,김상수,최정은,임환섭,김준명,이꽃실 대한감염학회 2004 감염과 화학요법 Vol.36 No.2

E. corrodens는 면역저하 환자에서 주로 문제를 일으키며, 감염시 두경부, 폐, 복부 등에 위험을 초래할 수 있어 치료의 적절성에 대한 중요성이 부각되고 있다. Eikenella corrodens는 균 동정을 위해 CO₂의 공급을 필요로 하며 서서히 자라는 특성상 까다로운 배양조건을 필요로 하는 균주이다. 혐기성 균주이지만 metronidazole이나 clindamycin과 같은 항균제에는 반응을 안하는 특이한 항생제 감수성에 의해 보편적인 구강내 상재균에 대한 경험적 치료에 실패할 수 있다. 치료에 있어 잘못된 초기 항생제 선택이나 외과적 시술이 적절히 시행되지 않은 경우에 치료실패의 요인이 될 수 있으므로 적절한 항생제 사용과 함께 외과적 처치가 동반되어야 한다. 저자들은 정상 면역력을 가진 성인에서 사람에게 물리고 난 후 발생한 E. corrodens에 의한 간농양의 적절한 치료의 결과를 문헌고찰과 함께 보고하는 바이다. Eikenella corrodens is a slow growing, facultatively anaerobic gram-negative rod that is part of the normal flora of the oral cavity and upper respiratory tracts. In most patients with E. corrodens infections, host defenses are compromised due to surgery, or chronic debilitating illness such as malignancies, or immunosuppressive therapy. This organism causes abscesses and infections that are at times fatal. We experienced a case of E. corrodens isolated from liver abscess in an immunocompetent patient. A 50-year-old man who is a dentist, was hospitalized because of fever and myalgia of 10 days' duration. Abdominal ultrasonography showed non-septated, 3.8×4.6 ㎝ sized abscess in the segment 6/7 of the liver. E. corrodens was isolated from aspiration of liver abscess. This organism was presumably acquired from his patient's oral cavity during dental procedure. We hope that increased awareness to E. corrodens will raise the index of suspicion, and contribute to optimal management of these infections, minimizing the significant morbidity associated with this organism.

Pseudomonas aeruginosa의 항생제내성에 관하여

高漢喆,朴政枰,朴悅 朝鮮大學校 附設 醫學硏究所 1989 The Medical Journal of Chosun University Vol.14 No.1

In order to study the MIC distribution and serotypes of p. aerugmosa, 112 strains isolated from two general hospital in kwangju city were tester). The following results were obtained. 1. Among 112 strains of p. aeruginosa isolated, 107 strains(95.5%) were typable. serotype E(30.4%) and G(30.4%) were most prevalent followed by B(11.6%), serotype A(7.1%) and serotype F, I(1.8%) among 107 typable strains. 2. According to test of MIC distribution on 17 antibiotics against p. aeruginosa. aminoglycosides were 0. 5~>512mcg/㎖, MIC peak in AK 32mcg/㎖ and other antimicrobial agents were > 512mcg/㎖. penicillins were 4~>512mcg/㎖, MIC peak in pi 256mcg/㎖ and other antimicrobial agents were >512mcg/㎖. Cephalosporines were 8~>512mcg/㎖, MIC peak in CP 64mcg/㎖ and other antimicrobial agents were>512mcg/㎖. 3. Among those antimicrobial agents, amikacin and rifampin showed good antimicrobial activity. 4. All of drug-resistant p. aeruginosa strains were multiply resistant to nine or more drugs. 35 strains were resistant to 17 drugs, follwed by 21 strains resistant to 15 drugs, 9 strains resistant to 14 drugs. 5. Out of 9~17 drugs, E. and G serotypes were 34 strains, out of 9~17 drugs, B sero-type were 13 strains, out of 13~]7 drugs, A serotype were 8 strains.