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?티스속근경과 탈지된 크로톤종자의 혼합추출물(CP-2)이 동물체내에서의 수종 암세포증식에 미치는 영향
문정조,김춘원,한영복,경홍기,홍은경,조영호,김종배 中央醫學社 1993 中央醫學 Vol.58 No.5
It has been reported that CP-2 extracted from the mixture of coptis and croton tiglium L. showed a cytotoxicity against various tumor cell lines. Based on this result, we have investigated furthre the effect of CP-2 on the growth of tumor cells in animal and in vivo characterization of combination antitumor chemotherapy with CP-2 and commercial chemotherapeutic agents. The most effective amount of CP-2 was 125 mg/kg in mice bearing P388 and L1210 scitic tumors, and S-180 and Ehrlich solid tumors. When the inhibitory effect on the growth of tumors were measured by tumor weight and size after inoculation with S-180 and Ehrlich cell lines, and administration of CP-2, it showed significant inhibitory effects against both cell lines. It inhibited also the growth of tumors in CDF1 mice inoculated with P388 and L1210 lines while administrations of croton tiglium L. extract along, coptis extract alone and only mitomycin-C did not show significant effects. It is interested that CP-2 enhanced the antitumor action of chemotherapeutic agents such as Picibanil, mitomycin-C and (3-interferon, suggesting that it contains a compound showing synergistic effect in addition to the compound with direct cytotoxicity to tumors, and the possibility that CP-2 can be used for a combination antitumor chemotherapy with other known chemotherapeutic drugs.
염화제이수은 및 중크롬산칼륨이 가슴샘의 미세구조에 미치는 영향
안의태,고정식,박경호,박인규,경홍기,한영복,Ahn, E-Tay,Ko, Jeong-Sik,Park, Kyung-Ho,Park, In-Kyu,Kyung, Hong-Kee,Han, Young-Bok 한국현미경학회 1997 Applied microscopy Vol.27 No.1
Ultrastructure of mouse thymus was evaluated, following the administration of potassium dichromate and mercuric chloride, the heavy metals of evironmental pollutants. Potassium dichromate (20 mg/kg) or mercuric chloride solutions (10 mg/kg) were subcutanously injected to the mice. Six hours, three days and two weeks after the injections, animals were sacrificed. Thymic tissues were fixed in 2.5% glutaraldehyde-1.5% paraformaldehyde solutions. The procedure was followed by the fixation in 1% osmium tetroxide solutions. Washed and dehydrated tissue-blocks were embedded in the araldite mixture. Ultra-thin sections were stained with uranyl acetate-lead citrate solutions. Results observed were as follows: 1. In electron microscopy, cortical population of thymocytes in the thymus of experimental groups were reduced. especially in the outer cortex. Subcapsular cortices of potassium dichromate treated mice were filled with many epithelial reticular cells, whereas the similar area of mercuric chloride-treated mice exhibited large intercellular spaces. 2. In the thymus of mercuric chloride treated group, large intercellular spaces were formed by shrinkage of epithelial reticular cells, and the space was invaded by numerous cytoplasmic projections of macrophages. Thymocytes nuded out from the shrunken cytoplasm of epithelial reticular cells, presented numerous microvilli. 3. In the thymus of potassium dicromate treated group, many activated macrophages and plasma cells migrated into thymic cortices. 4. In the perivascular spaces of thymic cortices of potassium dichromate- and mercuric chloride-treated mice, activated macrophages. plasma cells, collagen fibrils, and flocculent substance of exudated materials were exhibited. From the above findifgs, it was concluded that potassium dichromate or mercuric chloride could disturb the normal differentiation or 'education' of T cells in the thymic cortex. In turn, these heavy metals may hurt the immunological defense mechanism.
5-Fluorouracil 및 Mitomycin이 가슴샘의 미세구조에 미치는 영향
고정식,안의태,박경호,박동분,경홍기,한영복,Ko, Jeong-Sik,Ahn, E-Tay,Park, Kyung-Ho,Park, Dong-Boon,Kyung, Hong-Kee,Han, Young-Bok 한국현미경학회 1997 Applied microscopy Vol.27 No.1
The experiment was performed to study the morphological responses of the thymus of the mice, to antitumour agents (5-Fluorouracil or mitomycin C). Healthy adult mice weighing 25 gm each were divided into normal and experimental groups. 5-Fluorouracil (60 mg/kg) or Mitomycin-C $(400{\mu}g/kg)$ were injected subcutaneously to the animals every other day. Animals were sacrificed at 4 days and 7 days following the first injection. Pieces of the tissue taken from the thymus were prefixed with 2.5% paraformaldehyde-l.5% glutaraldehyde, followed by post-fixation with 1% osmium tetroxide. Ultrathin sections stained with uranyl acetate and lead citrate were observed with a JEM 100 CX-II electron microscope. The observed results were as follow: 1. Apoptoses of T-lymphocytes were observed more frequently in the thymus of the experimental groups than in those of a normal group. 2. In the experimental group, the plasma cells with distended cisternae of the granular endoplasmic reticulum and the eosinophile leukocytes were observed frequently. 3. In the experimental group, newly forming Hassall's corpurscles were observed frequently. 4. In the mitomycin-treated group, the epithelial reticular cells containing distended perinuclear cisternae, distended the granular endoplasmic reticula and pyknotic nuclei were observed in the cortico-medullary junctional area. 5. In the mitomycin-treated group, nuclear bodies with medium electron dense materials were often observed in the T lymphocyte. 6. In the 5-fluorouracil-treated groups, fused and dissolved tonofilament bundles and apoptotic bodies were observed in the some epithelial reticular cells in the medullary area. 7. In the 5-fluorouracil-treated groups, some elongated and bar-shaped lysosomes with electron lucent gap were often observed in the macrophages. 8. In the 5-fluorouracil-treated group, membrane complex of the smooth endoplasmic reticulum were ofen observed in the macrophage. From the above results, it was suggested that 5-fluorouracil or mitomycin could induce rapid involution of the thymus, and disturb maturation and differentiation of T lymphocytes, and, in turn, supress immunity.
?티스속 근경과 탈지된 크로톤 종자의 혼합 추출물(CP-2)이 수종 암세포에 대한 세포독성의 효과
김춘원,서경원,조영호,홍은경,한영복,문정조,경홍기,김정한,김종배 中央醫學社 1993 中央醫學 Vol.58 No.3
In order to develop anti-cancer drug with strong anti-cancer activity without side effects, we extracted by various steps from the mixture of Coptis chinensis (CO) and Croton tiglium L (CR), and evaluated it by measuring the cytotoxicity against various tumor cell-lines including normal spleen and thymus cells. Five murine cell lines including leukemia L-1210 and 9 human tumor cell lines including leukemia HL-60 derived from various organs such as stomach, liver, lung, colon and breast cancers were used. The cytotoxicity were measured by the well-known colorimetric [3-(4,5dimethylthiazol-2-yl)2,5 -diphenyltetrazolium bromice] (MTT) assay. The extract (CP-2) from the mixture of CO and CR were very effective to all tumor cell lines with different degrees but showed no significant effects on normal spleen and thymus cells. Specially stomach SNU-1, lymphoma Raji and colon SNU-C2A compared with others showed very strong cytotoxicity; 60? 80 %, 55 ?96 % and 40~80 %, within the range of 10 ~45 ?g/ ml, 10 ~90 ?g/ml and 55 ~96 ?g/ml of the extract (CP-2) respectively. The range of ID50 values were from 4.4 ?g/ml in lymphoma Raji to 350 ?g/mi in liver HEP-3B, which were relatively low compared with other natural crude extracts reported elsewhere.