질산화 작용이 있는 Aeromonas hydrophila의 동정 및 특성

엄미나,장재철,유영희,지의상 한국식품영양학회 2000 韓國食品營養學會誌 Vol.13 No.6

폐수처리 중 생물학적 처리에 활용할 수 있는 질소분해 능력을 가진 미생물을 분리하여 동정하고자 경기도내 하천 6지점에서 채취한 시료로부터 50개 균주를 선별하였다. 형태학적, 생화학적 및 배양학적 실험결과 Bergey's mannual of systematic bacteriology의 색인을 통하여 Aeromonas hydrophila로 동정하였다. Aeromonas hydrophila(AH-1). (AH-3), (AH-4), (AH-6) 균이 질산화 능력이 우수하였다. 4개 균주 모두 amoxillin, ampicillin, cephalothin과 ticarcillin에 내성을 나타내었다. 본 실험에서 분리한 .Aeromonas hydrophila의 질산화의 최적조건은 균 농도 1.0×l0exp(6) cells/ml, 배양온도 37℃로 나타났다. For the purpose of the isolation of microorganisms which have the capability of nitrification, we isolated the microorganisms in 6 samples collected from the stream of Kyonggi area. 60 strains were isolated. The selected strain were identified as a Aeromonas hydrophila based on the data obtained from the morphological, biochemical and cultural characteristics defined experiments. Among them Aeromonas hydrophila (AH-1), (AH-3), (AH-4), (AH-6) showed the highest nitrification capability. All isolates were resistant to amoxillin, ampicillin, cephalothin and ticarcillin. Optimum culture conditions of isolates were 37℃ and 1×10exp(8) cells/ml for 4 hours in the nitrate medium.

신농양에 동반된 척추 경막외 농양 1예

엄흥식,김준재,김현수,박영정,최상봉,박원도,김상현 인제대학교 2006 仁濟醫學 Vol.27 No.-

Spinal epidural abscess is an uncommon but readily diagnosable and treatable potential cause of paralysis and death. The incidence of spinal epidural abscess is approximately one to two cases per 10,000 hospital admissions in the United States. The symptoms of spinal epidural abscess are varied but include lower back pain, fever, local tenderness and neurological deficit, especially in such high risk groups as patients with diabetes, intravenous drug abuse, chronic renal failure, alcoholism, liver disease and immunocompromization. Infections of the epidural space originate from contiguous spread or via hematogenous routes from a distant source. Cutaneous sites of infection are the most common remote sources, especially in intravenous drug users. S. aureus accounts for most spinal epidural abscess infections, followed by streptococci and gram-negative anaerobes. We here report a case of spinal epidural abscess in renal abscess patient complaining of fever and back pain. A 71-year old female patient was admitted to epigastric pain and oliguria. She had been diagnosed as acute renal failure and treated with hydration. After third hospital days, she shows a persistent fever and drowsy mentality. Abdominal computed tomography demonstrated renal abscess. She was treated with intravenous antibiotics, third cephalosporin. Blood cultures and urinary culture revealed Escherichia, coli. After fourteenth hospital days, she developed back pain, muscle weakness and decreased sensation of lower extremities. Spine MRI demonstrated spinal epidural abscess with spinal cord compression. Surgical drainage with laminectomy was performed. After treatment of intravenous antibiotics and surgical drainage, she discharged with improved back pain and neurological symptom.

자연환경방사선에 의한 피폭선량(유효선량당량)의 종합적 해석연구

전재식,채하석,이병영,김현창,이동준,김도성,엄두하 충남대학교 기초과학연구소 1990 연구논문집 Vol.10 No.-

자연환경방사선의 세 가지 주요 요소인 지각방사선, 우주선 전리성분 및 라돈과 그 자핵종이 주는 선량을 측정, 해석하였는 바 18개월간에 걸쳐 대전, 수원, 경산 지역에 대하여 수행하고 이를 종합하여 유효선량당량으로 평가하였다. 지각방사선과 우주선 전리성분 측정을 위하여 3"∮×3" Nal(Tl) 섬광검출기와 휴대형 다중파고분석기를 이용한 감마선 분광분석 및 ^7LiF PTEE disk TLD에 의한 적산선량측정을 병행하였고, 공기중 라돈농도의 측정은 CR-39 고체비적검출기를 내장한 라돈컵을 설계제작하여 사용하였으며 비적수 라돈농도 교정인자는 스웨덴 국립방사선 방호연구소의 표준 라돈 챔버내에서 조사하여 결정하였다. 세 지역의 가연방사선에 의한 유효선량당량에는 약간의 수치적 차이는 있었으나 본질적 차이는 찾아볼 수 없었으며 지역 측정치의 분산가중 평균치는 지각방사선 (463±3)uSvㆍy^-1[(52.9±0.3)nSvㆍh^-1], 우주선 전리성분 (287±15)uSvㆍy^-1[(32.8±1.7)nSvㆍy^-1]로 외부 피폭이 (775±9)uSvㆍy^-1[(88.5±1.0)nSvㆍy^-1]였으며 라돈과 그 자핵종에 의한 내부피폭은 (1973±298)uSvㆍy^-1[(225.2±34.0)nSvㆍy^-1]였다. 따라서 자연황경방사선에 의한 여간 총 선량당량은 (2762±296)uSvㆍy^-1[(315.3±34.0)nSvㆍh^-1]로 평가되었는데 이 값들은 UN보고서^(11)에 나온 “정상적 자연방사선 지역”의 값과 비교할 만한 타당한 값으로 나타났다. Assessment of effective dose equivalent from natural environmental radiation that consists of terrestrial gamma­ray, ionizing component of cosmic­ray and alpha­rays of atmospheric radon and its decay products has been carried out for 18 months in Daejeon, Suwon and Kyungsan areas. A 3"∮×3"cylindrical Nal(Ti) scintillation detector in association with portable multichannel analyser and ^7LiF PTFE disk TLDs were used for the measurement of terrestrial gamma­ray and cosmic­ray ionizing component, while self­designed radon­cups with CR-39 SSNTD attached to the inside bottom were used for the integral measurement of at­mospheric radon concentration. Determination of calibration factor for converting track density of CR-39 detector to radon concentration was made by irradiation of radon­cups in the standard radon chamber of Swedish National Institute of Radiation Protection. Although resultant effective dose equivalents of three different measurment points show minor numerical differences, no essential discripancy between the places was found. The variance weighted means of the annual effective dose equivalents of each component of radiation from the tree places came out to be: (463±3)uSvㆍy^-1[(52.9±0.3)nSvㆍy^-1] for terrestrial gamma­ray, (287±15)uSvㆍy^-1[(32.8±1.7)nSvㆍy^-1] for ionizing component of cosmic­ray and (1973±298)uSvㆍy^-1[(225.2±34.0)nSvㆍy^-1] for internal exposure of radon and its decay products. Consequent total effective dose equivalent turned out to be (2762±298)uSvㆍy^-1[(315.3±34.0)nSvㆍy^-1], which if reasonably comparable with that appeared in UNSCEAR Report^[11]as per caput annual effective dose equivalent in “areas of normal back ground radiation.”

조리방법에 따른 식품 중 영양소 잔존률(retention factor)에 관한 연구(Ⅰ) : 채소류 및 감자류 Vegetablesn ane potatoes

김대병,장영미,김인복,이혜영,장재희,박정현,엄세정 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

조리조건에 따른 식품의 영양소 잔존를을 측정하여 보다 정확한 조리식풍에 의한 영양소 섭취량을 평가할 수 있도록 하기 위하여 합리적인 영양소섭취량 조사, 영양 평가를 위한 개선책을 제시하고자 끓였다. 대상 시료와 조리법은 시금치(데치기, 끓이기), 배추(끓이기), 콩나물(데치기, 끓이기), 호박(끓이기, 볶기). 당근(끓이기, 볶기), 우엉(조림), 감자(끓이기, 볶기)이었으며, 조리 전 후의 비타민 A(β -carotene), Bl, B2, C, E, 나이아신, 칼슘, 철, 아연, 칼륨, 인, 나트륨의 함량을 분석하여 조리법에 따른 잔즌률을 국하였다. 조리조건별 잔존률을 구한 결과 무기질은 데치기의 경우 조리수를 제거하므로 손실이 있었으나 끓이기, 볶기 등의 조리법에서는 손실이 거의 없었다. 비타민 A와 I는 대부분 80%이상으로 많이 손실되지 않았으며 비타민 Bi은 데치기가 가장 낮았고, 조림, 괄이기, 볶기의 순으로 잔존률이 높았다. 비타민 B2와 나이아신은 비타민 Bi보다는 잔존를이 높은 경향이었다. 비타민 C는 대부 분의 조리법에서 크페 손실되었다. 본 연구의 결과는 합리적이고 정확한 영양소 섭취량 평가를 위한 기초자료로서 기존의 평가방법을 개선 보완하여 영양소섭취량 평가으 과학화에 기여할 것으로 기대된다. In various mutrition activities, the necessary values of nutrient intake were usually calculated from food composition tables which have nutrient contents of raw ingredients and dishes, but Korean food composition tables of dishes were lacking, So we studied todetermine the nutnent retention factors(RF) of vegetables and potatoes by cooking methods. Atfirst, we surveyed cooking methods of vegetables and potatoes, and selected major vegetablesand cooking methods. Selected items and cooking methods were sp.mach(blanching and boiling),cRnese cabbage(boiling), soybean sprout(bBanchlng and boiling), pumpkin(boiling andstir-frying), carlot(boiling and stir-frying), burdock(braising), and potato(boiling and stir-frying)We analyzed 6 vitamins luhich were vitamin A(β-carotene), Bl, B2, C, E, and niacin, and 6minerals which were calciurrl, iron, zinc, potassium, phosphorus, and sodium. The results wereas follows : Minerals almost retained in boiling, stir-frying, except in blanching method, RF ofvitamin A and I were 80% or more. RF of vitandn Bl were decreased in order of sfir-frying,boiling, braising, blanching and the RF of vitamin B2 and niacin were higher than vitandn Bl,RF of the vitamin C were varird in So~90% in all cooking methods

자가 탈회골의 조직반응에 관한 실험적 연구

김장연,김영조,엄인웅,이동근,이재은 大韓顎顔面成形再建外科學會 1993 Maxillofacial Plastic Reconstructive Surgery Vol.15 No.3

Many surgeons are on the point of bone excision and reconstruction of the bone defects by autograft. xenograft, and allograft in the treatment fo begin and malignant tumors of bone. Of all type of bone grafts, we received the autograft as the best ideal bone graft. Of autogenic bone graft, replantation of excised autogenic bone for reconstructiong the bone defects has been the ideal method until now, but early bone healing reponses and tumor cell devitalization after replantation of excised autogenic bone have not been identified for clinical applications. So, to evaluate bone healing response after replantation in rabbit's calvarial bone, we divided the experimental group into three groups. Group 1 is a fresh autogenous bone group. Group 2 is a deep frozen group. Group 3 is freeze-dried demineralized group. Obtained result were as followed: 1. Inflammatory cell infiltration appeared at I week and disappeared at 4 weeks in all experimental group, Especially, severe inflammatory cell infiltration showed in fresh autogenous bone group at 2 weeks, Especially, severe inflammatory cell infitration showed n fresh autogenous bone group at 2 week Group 3 is the least showing group on the point of inflammatory cell infiltration. 2. Osteoblastic activity evenly increased upto 4 weeks and maintained to 6 weeks and decreased after this period, especially osteoblastic activity in group 2 is less than group 1 and group 3. We can't discriminate between osteoblastic activity of group 1 and that of group 3. 3. In new bone formation, group 3 was more active than any other groups at early stage, but there were little differences among three experimental groups at later state. 4. Bone resorption around the grafted bone slightly appeared at 1 week and disappeared at 4 weeks in all experimental groups. We can find the more bone resorption in group 2 at 2 weeks than any other groups. We could suggest, as appears from our results, that freeze-dried deminiralized bone graft is the useful bone graft in the clinical applications of excised autogenic bone.

관상동맥경화증 환자에서 전곡섭취가 지질과산화 및 혈중 인슐린과 호모씨스테인 농도에 미치는 영향

장양수,이종호,엄영람,조은영,바현영,황재관,여익현 대한순환기학회 2000 Korean Circulation Journal Vol.30 No.6

TNFα -308 and TNFβ +252 locus plays an important role in the etiopathogenesis of cerebral infarction

Hong Seung-Heon,Kim Hyung-Min,Um Jae-Young 대한약학회 2004 大韓藥學會 總會 및 學術大會 Vol.2004 No.2

INTERLEUKIN-1 RECEPTOR ANTAGONIST GENE POLYMORPHISM AND TRADITIONAL CLASSIFICATION IN OBESE WOMEN

UM, JAE-YOUNG,KIM, HYUNG-MIN,MUN, SUNG-WON,SONG, YUNG-SUN,HONG, SEUNG-HEON Taylor Francis 2006 INTERNATIONAL JOURNAL OF NEUROSCIENCE - Vol.116 No.1

<P>Cytokines appear to be the major regulators of adipose tissue metabolism. Interleukin- 1 receptor antagonist (IL-1ra) serum levels are increased in human obesity, and are under strong genetic control. The hypothesis was tested that the IL-1ra gene might be a candidate for obesity. Furthermore, the relationship was investigated between Sasang constitution and IL-1ra polymorphism. The frequency of a penta-allelic 86-bp tandem repeat (VNTR) in the intron 2 of IL-1ra gene in 67 lean (BMI 2 ), 133 overweight (BMI 25-29.9 kg/m 2 ) and 61 obese (BMI ≥ 30 kg/m 2 ) otherwise healthy Korean subjects was investigated. Total fat mass and percentage body fat were determined by dual-energy X-ray absorptiometry. Subjects were discriminated into four types by QSCC II program as well as clinical data (weight, height, blood pressure, etc.); Teaeumin, Taeyangin, Soyangin, and Soeumin. Genomic DNA was extracted and used for polymerase chain reaction-based genotyping of IL-1ra. The genotypic, or allelic distribution did not differ markedly between the three groups. The relative risk of being obese in comparison with lean was twofold increased in allele 2 carriers, although it was not statistically significant. Carriers of the allele 2 did not show a significant difference in physical and clinical characteristics. However, the relative risk of being obese in comparison with lean was increased in Taeumin subjects ( p = .050), and so was in IL-1ra A2- carriers ( p = .047). No relationship was found between the IL-1ra polymorphism and BMI in Korean women, but the authors first attempted to find an association among IL-1ra polymorphism, obesity, and Sasang constitution.</P>

A Prospective, Multicenter Study to Evaluate the Efficacy of Terlipressin and Albumin in Cirrhotic Patients with Acute Kidney Injury

( Jae Yoon Jeong ),( Joo Hyun Sohn ),( Yong Kyun Cho ),( Ji Hoon Kim ),( Young Kul Jung ),( Soon Ho Um ),( Sang Bong Ahn ),( Chang Hyeong Lee ),( Jae Young Jang ),( Young Seok Kim ),( Hong Soo Kim ),( 대한간학회 2017 춘·추계 학술대회 (KASL) Vol.2017 No.1

Aims: To compare the efficacy of a combined treatment with terlipressin and albumin (treatment group) with albumin alone (control group) in cirrhotic patients with ascites and acute kidney injury (AKI) between the values of serum creatinine (sCr) 1.5 and 2.5mg/dl after 2 or 3 days of a conservative treatment. Methods: Among randomized 40 patients, 34 were included in this interim analysis. For the treatment group (n=13), terlipressin was intravenously given at a total dose of 4mg/day in conjunction with albumin 20g/day. In case of sCr>1.5mg after 3 days of the treatment, the dose of terlipressin was doubled to 8mg/day. For the control group (n=21), during 5 days of treatment the same dose of albumin 20g/day was given. The primary outcome was AKI recovery rate after 5 days of the treatment. Recovery is arbitrary defined as a decrease of sCr ≤1.5mg or by a reduction of sCr ≥50% from the pretreatment value. Secondary outcomes were: rate of type 1 hepatorenal syndrome development, AKI partial recovery rate (sCr>1.5 and a reduction of sCr<50% from the pretreatment value), survival rate at week-4 follow- up after treatment initiation, AKI recurrence rate at week-4 and adverse effects of therapy. Results: AKI recovery rate was 77% in the treatment group and 33% in the control group (p=0.017). AKI progression and HRS development occurred only in the control group, 19% and 5% respectively, whereas did not in the treatment group. Treatment with albumin alone was ineffective in 67%. AKI recurrence rate was about 60% in both groups with no difference. One death occurred in the control and 2 in the treatment group, due to hepatic failure. Conclusions: A combined treatment with terlipressin and albumin is more effective than albumin alone in cirrhotic patients with ascites and AKI with serum creatinine between 1.5 and 2.5 mg/dl.

Discrimination between genetically identical peony roots from different regions of origin based on 1H-nuclear magnetic resonance spectroscopy-based metabolomics: determination of the geographical origins and estimation of the mixing proportions of blende

Um, Jung A,Choi, Young-Geun,Lee, Dong-Kyu,Lee, Yun Sun,Lim, Chang Ju,Youn, Young A,Lee, Hwa Dong,Cho, Hi Jae,Park, Jeong Hill,Seo, Young Bae,Kuo, Hsun-chih,Lim, Johan,Yang, Tae-Jin,Kwon, Sung Won,Lee, Springer-Verlag 2013 ANALYTICAL AND BIOANALYTICAL CHEMISTRY Vol.405 No.23