담도 폐쇄증 환아의 간경변 조직에서 유전자 발현의 변동

최병호,이현미,김문규,김정철 경북대학교 병원 2002 경북대학교병원의학연구소논문집 Vol.6 No.1

목적:간외 담도 폐쇄증은 소아영역에서 간이식의 적응증으로 가장 많은 부분을 차지하는 질환으로 서 발생원인은 아직 밝혀져 있지 않으며, 간외 담도의 진행성 소실을 설명하기 위한 많은 가설이 제시되어 있으나 증명되지 않았다.따라서 담도 폐쇄증의 원인과 발생과정을 이해하고 조기 진단을 위해 발생과 진행,간섬유화 등에 관여하는 가능한 유전자의 규명은 매우 의미있는 시도이다. 방법:인간 모유두 새포로부터 구축한 cDNA ilbrary를 이용하여 dot blot panel을 제작하였다. 생체부분 간이식 수술시 환아가 간경변 조직과 공여자의 정상 간조직을 얻은 후 각각의 조직으로부터 RNA를 분리한 후 역전사하여 cDNA를 얻었다.rendom primed DNA labelling방법을 이용하여 방사능 동위원소를 붙임으로써 방사능 표지된 cDNA probe를 만들었다.membrane을 30분간 prehybridization 시킨 후 1시간 동안 hybridization 시켰다.reverse dot hybridization 결과 담도 폐쇄성 간경변 조직과 정상 간 조직에서 차이나는 클론을 확인하고 해당 클론의 핵산 염기서열을 분석하였다.그 결과를 BLAST를 이용하여 GenBank의 database와 비교 검색함으로써 선택된 클론이 기존의 어느 유전자와 상동성을 가지는지 확인하였다. 결과:담도 폐쇄성 간경병 조직에서 발현이 증가한 유전자는 26종으로서 bcl-w,laminin binding protein, hepatocyte growth factor-regulated tyrosine kinase substrate(HRS), tymosin β-4,10,transforming growth factor(TGF)-β, tissue inhibitor of metalloproteinase(TIMP)-1,signal recognition particle(SRP)4, eukaryotic initiation factor(eIF)-2 α kinase,lysyl oxidase, aldolase A, γ-glutamylcystein synthetase,collagen typeⅠ α1,2,collagen typeⅢ, fibronectin, osteonetin, insulin-like growth factor binding protein(IGFBP)2,3 등이었다.담도 폐쇄성 간경변 조직에서 발현이 감소한 유전자는 gastrula zinc finger protein과 novel gene:K0059 이었다. 결론:이 연구는 담도 폐쇄증과 간섬유화의 특이유전자 규명으로 담도 폐쇄증과 간섬유화의 원인규명에 기여하고 질환의 조기 발견과 간경화의 치료와 예방 물질의 개발을 위한 기초 자료가 될 것으로 생각한다. Purpose: Extrahepatic biliary atresia is the most common indication for liver transplantation in children. Serveral studies have been explain the destructive, inflammatory process leading to fibrosis and obliteration of the biliary tract, but the etiology of this disorders remains unknown. It would be very significant to identify genes that are specifically expressed in pathologic liver tissue of biliary atresia and analyze the pattern of expression in those genes. Method: We made dot blot panels consisting of 1,730 different EST(expressed sequence tags) clones which were isolated from human hair dermal papilla cell cDNA library.Liver tissues were taken from a recipient with biliary atresia and a normal donor during living-related liver transplantation.Total RNA was extracted from each sample and reversely transcribed to make cDNA. Then radioablelled cDNA probe pools were made by random primed DNA labeing method and used for screening differentially expressed genes using EST dot blot panel. Results: Among the total of 1,730 EST clones, 26 cDNA clones were overexpressed in biliary cirrhosis.They revealed homology to genes encoding bcl-w, laminin binding protein, hepatocyte growth factor-regulatef tyrosine kinase substrate(HRS),thymosin β-4, 10; transforming growth factor(TGF)-β, tissue inhibitor of metalloproteinase(TIMP)-1, signal recognition particle(SRP)4, eukaryotic initiation factor(eIF)-2αkinase, lysyl oxidase, aldoase A, γ-glutamylcystein synthetase, collagen typeⅠα1,2,collagen typeⅢ,fibronectin,osteonectin,insulin-like growth factor binding protein(IGFBP)-2,3,and more. In addition, the expression of 2 clones ahowed that gastrula zinc finger protein and one novel gene were decreased in biliary atresia. Conclusions: This study identified differential EST screening technique.We believe this studt could lead to a better understanding of the pathogenesis of biliary atresia and hepatic fibrosis.(J Korean Pediatr Soc 1999;42:1-10)

圖書利用頻度 分析에 의한 學生利用 圖書量測定에 관한 硏究 : 大學圖書館 學部學生을 中心으로

崔達鉉,孫正彪 慶北大學校 文理科大學 1975 文理學叢 Vol.3 No.-

This study is an attempt to present a model for a more reasonable, economical standard of building a library collection for undergraduates students. On the basis of various circulation data of external and internal literature, the following methods of calculation for the study were employed: 1) A quantitative standard for undergraduate students was calculated by the exponential function derived from Trueswell's figure % of Circulation satisfied vs. % of Holdings satisfying circulation and Leimkuhler's hypothetical table for Relative use of stored items. 2) A standard for the annual increase of the collection was made by a formula derived from the principle of half-life in nuclear physics. 3) A statistical method for the measurement of duplication were formulas applied by the standard deviation and the coefficient of variation. The scope of this study was set up quantitatively by the number of students and limited to the circulation of books taken-out in the general collection. Other standards were excluded. The result of this study is as follows: First, the setting up the quantitative standard by solving of an equation of exponential function. C_q=K^avt_e, derived from the figure by Trueswell and Leimkuhler, revealed that colleges and universities will have to maintain the following ration of books to undergraduate students: 1) The minimum-more than 9 volumes per student. 2) The optimum-more than 14 volumes per student. Secondly, the standard of the annual increase of collection, a result of calculating the formula, N=N_oe^-λT, derived form the principle of half-life in nuclear physics, shows that colleges and universities will have to maintain the following ratio of books to undergraduate students: 1) The minimum-more than 0.62 volumes per student. 2) The optimum-more than 0.97 volumes per student. Thirdly, concerning the increase in student demand for duplication, colleges and universities will have to maintain a level of duplication to be calculated by the following formula derived from the standard deviation and coefficient of variation: 1) The quantity satisfying 99.73%…X_199.73%=X_1av+3×S/X×X. 2) The quantity satisfying 99.45%…X_199.45%=X_1av+2×S/X×X.

大學 圖書館 年間 圖書費 豫算策定에 관한 硏究 : 年次 增加量에 의한 理論的 模型

崔達鉉,孫正彪 경북대학교 교육대학원 1976 논문집 Vol.6-7 No.-

This study is an attempt to present a model for a more reasonable, economical standard of an annual budget for the university and college library collection. The annual budgetary model for the annual increase of collection was calculated by a formula derived from a factor analysis of the fluctuations of external and internal mean price indexes. The result of this study is as follows: ⑴ The method of calculation for the study was employed by the following formula: For Korean books, B_Tn=V(c_(n-1)+C_(n-1)×33.8/100)……………………………………………………⑴ For Japanese books, B_Tn=V(c_(n-1)+C_(n-1)×19.2/100)×(E_(n-1)+E_(n-1)×22.7/100)………… ⑵ For western books, B_Tn=V(c_(n-1)+C_(n-1)×11.2/100)×(E_(n-1)+E_(n-1)×7.1/100) ………… ⑶ Total ⑴+⑵+⑶ ⑵ The result by the above formula is shown to 8,000 Won per student for 1975. Accordingly, the annual budget in future will have to be calculated by adding the rate of the price increase on the above result.

Mycobacterium abscessus 5례

김정현,최원호,강정옥,최태열 대한임상미샐물학회 2004 Annals of clinical microbiology Vol.7 No.1

都市圈 公共圖書館 分館시스팀計劃에 관한 硏究 : 釜山直轄市를 對象으로 한 理論的 模型 Theoretical Model for Branch Library System Planning in Pusan City

孫正彪,崔達鉉 경북대학교 사회과학대학 1982 社會科學 Vol.1 No.-

This study is an attempt to present a model of branch library system planning of the public library in Pusan City. This model is built up through the analysis of the distribution of population and characteristics of community structure, based on the standard for the branch libiary system of a public library. The results are summarized as follows: 1. Under the circumstances of this country, each radius of the effective service area for branch libraries is as follows: 1∼1.5㎞ is adequate for the central library and major branches; 1㎞ for neighborhood branches; 0.5∼0.8㎞ for sub-branches. 2. Population within the radius of the effective service area of a given branch may range from 80,000 to 100,000 for major branches, from 50,000 to 60,000 for neighborhood branches, and from 20,000 to 30,000 for sub-branches. 3. The radius of the outer boundary for the effective service area may be 9㎞ from the heart of the city, and that of the inner boundary, 1.3㎞. 4. Areas within the width of 3㎞ toward both the east and west from the heart of the city, along the direction of the southwest and northeast, may be selected as the most effective locations for branch libraries, and between 3㎞ and 4㎞ as the second priority, and between 4㎞ and 5㎞ as the third. 5. The number of branches may be 29, that is, 7 major branches (Dongsang, Yangjung, Bumil, Daeyun 6 dong, Kwangan, Ami, Sinsun), 11 neighborhood branches (Myungryun, Yoensan 5 dong, Yoensan 3 dong, Buam, Danggam, Sujung 3 dong, Gaegum, Gamman, Joongang, Nambumin, Chunghak), and 11 sub-branches (Sajik 3 dong, Sajik 2 dong, Yoensan 7 dong, Bumchun, Namchun, Banyoe, Daeyun 1 dong, Guejung, Dukpo, Gamjun, Daejo)

한국형 노인주거개발을 위한 이론적 고찰

박유복,최영선,김현정,오이진,박정희 목포대학교 생활과학연구소 2000 생활과학논집 Vol.3 No.-

According to the increase of the number and portion of the elderly people, the elderly housing is an important social welfare factor as the solution of the elderly problem. When we consider the elderly housing of Korea, it should be appropriate to Korean culture and emotion. This study aims to suggest two ways of Korean elderly housing: three-generation housing and the renovation of housing which one elderly person have lived in. Considering that family centrism is remained a lot in Korean consciousness, those two ways should be developed well so as to build a unique Korean housing culture for the elderly.

학교와 학원의 비교를 통해 본 청소년의 플로우 및 내적경험

김기옥,현은자,최인수,유현정 대한가정학회 2004 Human Ecology Research(HER) Vol.42 No.4

The purpose of this study was to compare the school life of Korean high school students between public and proprietary schools, especially focused on their internal experience and flow. Internal experiences were composed of three dimensions: emotion, cognitive efficiency, and satisfaction. Flow was classified into four conditions: apathy, boredom, anxiety, and flow. The study results are: 1. Korean high school students felt more flow when they were in public school than in proprietary school,. In public school, they felt relatively more bored, and on the other hand, they were relatively more anxious in proprietary school. 2. Korean high school students felt more positive emotions when they were in public school than in proprietary school. 3. Korean high school students were more involved into study when they were in public school than in proprietary school. 4. Korean high school students were more satisfied when they were in public school than in proprietary school.

두가지 혈류 유발방법에 의한 새로운 심폐소생술(이중 혈류 유발 심폐소생술)장치의 개발

황성오,김현,조준휘,오범진,임종천,최경훈,윤정한,이승환,김영식,이강현,이윤선 大韓應急醫學會 1999 대한응급의학회지 Vol.10 No.2

Background: There have been many efforts to augment blood flow during cardiopulmonary resuscitation. These efforts have focused on maximizing the effect of cardiac pump or thoracic pump alone. However, considering that the heart is the biggest blood reservoir and increase of intrathoracic pressure can generate blood flow, simultaneous exploitation of both mechanisms may have synergistic effect. We hypothesized that simultaneous chest constriction in addition to sternal compression by standard CPR may have additive hemodynamic effects by preventing deformation of the chest and increase of intrathoracic pressure. Methods and results: we built a new mechanical device to perform compression and thoracic constriction simultaneously. The device consists of two main elements. Piston in the center is to depress the sternum. Strap is to constrict the thorax circumferentially. Strap is attached to both sides of the piston. When the piston is pushed down, it depresses the sternum and pulls on the thoracic strap. To determine strap width to produce optimal hemodynamic effect, we measured hemodynamic parameters with variable widths of strap in two dogs after induction of ventricular fibrillation. Result of the experiment showed that 10cm wide strap was determined to be most effective. We also determined optimal depth of compression to produce maximal hemodynamic effect with animal experiments using two dogs. Animal experiments showed that the highest aortic pressure could be generated when the stemum was depressed to 5 cm. Cardiopulmonary resusciation using a new device could generate higher systolic aortic pressure, coronary perfusion pressure and end-tidal carbon dioxide tension in comparison with standard cardiopulmonary resuscitation in a pilot animal study using two dogs. Conclusion: New cardiopulmonary resuscitation method using a mechanical device designed by us could perform sternal compression and simultaneous thoracic constriction, and generate better hemodynamic effects than standard cardiopulmonary resuscitation in pilot animal experiments.

류마티스 관절염 환자에서 발생한 Mycobacterium intracellulare에 의한 관절염 1예

박근우,권현희,정승혜,김경찬,최정윤,이영환 대한감염학회 2007 감염과 화학요법 Vol.39 No.1

비정형 항산균에 의한 근골격계 감염은 매우 드물며, 진단이 지연되는 경우가 많다. 저자들은 류마티스 관절염환자에서 수차례 스테로이드 주사 후에 생긴 M. intracellulare에 의한 관절염을 진단하고 수술적 치료없이 약물 치료만으로 좋은 결과를 보인 예를 경험하였기에 보고하는 바이다. Nontuberculous mycobacteria are ubiquitous organisms that are frequently present in the water, soil and animal reservoirs. Nontuberculous mycobacterial infections of the musculoskeletal system are rare and usually associated with predisposing factors, such as prior joint disease, trauma, use of intraarticular or oral corticosteroids, or an immunocompromised state. A sixty five-year-old patient with rheumatoid arthritis was hospitalized due to swelling on the left wrist. M. intracellulare was cultured from the aspirated joint fluid. The patient was successfully treated with clarithromycin, ethambutol, and rifampin. We report this case with review, emphasizing high suspicion for nontuberculous mycobacterial infection in patients with predisposing risk factors.

이차성 기질화 폐렴이 동반된 피부근염 1예

박철연,권정석,정진욱,이충기,현대성,최정윤 영남대학교 의과대학 2008 Yeungnam University Journal of Medicine Vol.25 No.2

Dermatomyositis is characterized by progressive, symmetric, Proximal muscle weakness and a nonsuppurative inflammatory myopathy of unknown etiology involving predominantly skeletal muscles. It is also characterized by typical skin lesions. Interstitial lung disease has a poor prognosis when it is associated with dermatomyositis. Organizing pneumonia is a disease in which granulation tissue fills the lumina of terminal and respiratory bronchioles and extends into the distal airspaces. The cryptogenic nature of the process is appreciated in that organizing pneumonia patterns of injury can be seen in secondary forms of the disease (secondary organizing pneumonia). Organizin pneumonia has been reported to occur in 5~10% in dermatomyositis-polymyositis patients Anti-histidyl tRNA synthetase antibody (anti-Jo-1) is a Predictive disease marker that is reported to occur in up to 70% of patients. We describe a 49-year-old male dermatomyosits patient who presented with organizing pneumonia and was found to have negative anti-Jo-1 antibody.