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Canine Distemper Virus 검출을 위한 정량 Real-time PCR
이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2
Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.
강병노 ( Byeongro Kang ),권행운 ( Hang Woon Gwan ) 국민대학교 사회과학연구소 2020 社會科學硏究 Vol.33 No.1
본 연구는 미국의 복지국가의 역사적 발전과정을 분석하여 한국에 대한 함의를 도출하는 것을 목적으로 한다. 신자유주의 시대의 미국은 규모가 크지만 사적이며 잔여적이고 시장중심의 작은 복지국가라고 알려져 있다. 구체적으로 미국의 복지국가 변천과정을 태동, 형성과 발전, 재편과 개혁, 갈림길의 역사적 시기별로 분석한다. 분석결과, 미국 복지국가의 형성과 발전은 단면적이라기보다는 이중성, 양면성을 포함하였다. 미국은 간접적이며 소극적이고, 잔여주의적인 복지의 역사적 주변화 과정을 보였으며 분배, 권리, 국가책임, 주체와 연대 형성, 이민자의 문제 등이 잠재되어 있었다. 역사적 시기별로 확대와 축소가 반복되면서 개인의 자유와 책임을 중요시하는 개인주의와 자유주의적 전통은 보편적인 사회보장과 역사적 긴장관계를 형성하였다. 분석 결과를 토대로, 한국에 함의로써 복지국가를 위한 지속가능한 역사적 과제를 제안한다. The purpose of this study is to analyze the historical development of welfare states in the United States and to find implications for Korea. It is widely known that the development of welfare states in the United States is big size, private, residual, market-oriented, and small welfare policy in neoliberal globalization era. For the specific chronological method, the historical stages include the beginning, formation and development, reorganization and reform, and crossroads. The analysis results showed that the U.S. is an insecure and residual welfare state based on liberalism and individualism. The formation and development of the U.S. welfare state included duality and ambivalence rather than cross-sectional. The main process involves historical circumscription, passive social policy, and personal freedom and responsibility rather than social protection within as historical periods expand and decrease. Based on the results, this study proposes a sustainable task for welfare state in Korea.
복지국가 발전 동인과 전망에 대한 비교사회정책적 재발견
강병로 ( Byeongro Kang ),권행운 ( Hang-woon Kwan ) 국제지역학회 2017 국제지역연구 Vol.21 No.3
본 연구는 복지국가의 발전 동인을 비교사회정책적 관점에서 재발견하여 한국에의 함의를 모색하기 위한 목적을 가지고 있다. 복지국가 발전은 여전히 진행 중이지만 신자유주의적 지구화에 의한 자본축적으로 인해 그 경로와 전망은 불안정하며 복지국가적 발전의 방식에 대한 비판적 검토가 필요하다. 구체적인 국가별 비교를 위해서 영국, 미국, 스칸디나비아(스웨덴, 노르웨이, 핀란드, 덴마크), 남아메리카(칠레, 브라질, 아르헨티나)를 비교분석하였다. 분석결과, 영국은 사회적 보호의 전통에 의한 늙고 전통적인 복지국가, 미국은 자유주의와 개인주의를 바탕으로 불안전한 잔여적 복지국가, 스칸디나비아는 사회적으로 합의된 복지국가, 남아메리카는 불안정한 체제와 취약한 복지국가의 특성을 보여주고 있다. 국가별 비교 분석 결과를 토대로, 한국에 함의로써 기본자산 보장, 공정한 사회에서의 정의와 자유, 국가의 역할 재정립, 자본축적의 통제와 관리, 사회적 권리향상을 위한 사회개혁 등 포괄적인 신복지기획을 제안하였다. The purpose of this study is to rediscover the development motives of welfare states from the viewpoint of comparative social policy and seek implications for Korea. The development of welfare states is still in progress, but the path and prospects are unstable due to capital accumulation by neoliberal globalization, and a critical review of the welfare state development approach is needed. For the comparison between specific countries, the U.K, the U.S, Scandinavia (Sweden, Norway, Finland, Denmark), and South America (Chile, Brazil, and Argentina) were compared and analyzed. The analysis results showed that the U.K. is an old and traditional welfare state by the tradition of social protection, the U.S. is an insecure and residual welfare state based on liberalism and individualism, Scandinavia is a welfare state by societal corporatism and South America is characterized by an unstable regime and a vulnerable welfare state. Based on country-specific comparative analysis results, this study proposed a comprehensive new welfare plan such as guarantee of basic assets, justice and freedom in a fair society, reestablishment of the role of the state, control and management of capital accumulation, social reform to improve social rights as implications for Korea.
Sang-Yeol Kim,Woon-Ha Hwang,Sung-Hwan Oh,Jong-Hee Lee,Jun-Hyeon Cho,Kuk-Hyun Jeong,Ji-Yoon Lee,Sung-Tae Park,You-Chun Song,Un-Sang Yeo,Jeong-Il Kim,Hang-Won Kang 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
Experiments were conducted to investigated the change of primary dormancy and viability of Chinese milk vetch(CMV) seed buried in soil both as seed and pod with seeds under CMV-rice cropping system during the period of 2007~2009. The freshly harvested CMV seed alone and pods with seeds were buried in rice field at 0, 5 and 10cm depths and determined change of seed dormancy and viability at one to three month intervals for 10 months. The CMV seed had high dormancy of 95%, showing only 4~5% germination at the beginning in June but the seed dormancy was gradually broken at rice harvest time in autumn, showing 25 to 35% for seed and 55 to 61% for pod with seeds. The viability loss was faster in the seed than in the pod with seeds regardless of depths of placement in the soil base on decayed seeds. Also the seed placed on the soil surface lost viability faster than the 5~10 burial depths. However, appreciable number of CMV seeds still remained at even 4 months after burial in soil. These results indicate that seed dormancy was enable CMV plant to regenerate naturally from the remained soil seed bank at rice harvest time in autumn.
Thermosensitive and mucoadhesive delivery systems of mucosal vaccines
Han, In-Kwon,Kim, Young Bong,Kang, Hung-Sik,Sul, Donggeun,Jung, Woon-Won,Cho, Hee Jeong,Oh, Yu-Kyoung Elsevier 2006 Methods Vol.38 No.2
<P><B>Abstract</B></P><P>Mucosal vaccination is emerging as a potential administration route for eliciting antigen-specific mucosal and systemic immunogenicity. Most mucosal vaccines have been administered in a phosphate-buffered saline vehicle that may limit the exposure of antigens to the mucosal surfaces and result in poor immunogenicity. To improve the potency of the mucosal vaccines, we have developed mucosal vaccine delivery systems that might prevent leakage and increase retention of vaccines on mucosal surfaces. Thermosensitive polymers have been used to reduce the leakage problems of nasal or vaginal vaccines, while mucoadhesive polymers have been employed to increase the mucosal contact of the vaccines. Here, we describe the formulation and delivery methods of mucosal vaccines using thermosensitive and mucoadhesive polymers.</P>
Staphylococcus lugdunensis에 의한 감염성 심내막염 1예
최성호,박현구,변승운,구동회,강호석,장항제,김양수,우준희,김영휘,최상호 대한감염학회 2006 감염과 화학요법 Vol.38 No.5
Staphylococcus lugdunensis is a coagulase-negative staphylococcus (CNS) that is commonly found on the human perineal skin. Contrary to other CNS, S. lugdunensis is a rare contaminant in cultures and has the potential to cause clinically significant infections, including infective endocarditis. Infective endocarditis due to S. lugdunensis have been known to resemble endocarditis due to S. aureus because of its aggressive clinical course and high mortality. We report a case of infective endocarditis caused by S. lugdunensis in a 21-year-old woman. She was cured of the infection with surgical and antibiotic therapy. S. lugdunensis에 의한 감염성 심내막염은 국내에서보고가 없었던 매우 드물지만 다양한 합병증과 높은 사망률을 보이는 감염증이다. 저자들은 S. lugdunensis가 수술적 치료와 항생제 치료로 완치된 감염성 심내막염 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다.