침 시술로 발생한 Non-O1, Non-O139 Vibrio cholerae에 의한 패혈증

임태섭,지아영,이중희,장수연,김인수,김영주,김범경,김승업,박준용,안상훈,한광협,김도영 Ewha Womans University School of Medicine 2013 EMJ (Ewha medical journal) Vol.36 No.S

Vibrio cholerae is mainly known to cause gastrointestinal infection after seawater exposure or raw seafood intake. It is rarely reported to cause cellulitis or sepsis, but threre has been no known case after acupuncture. Herein, We report a 56-year-old cirrhotic patient of non-O1, non-O139 Vibrio cholerae septicemia caused by cellulitis of both lower extremities after acupuncture.

치근단 병소의 임상적 연구

김상우,우순섭,유임학,이영수,심광섭 대한악안면성형재건외과학회 2001 Maxillofacial Plastic Reconstructive Surgery Vol.23 No.3

The purpose of this study was to find the clinical features of periapical lesions. A total of 130 periapical lesions which were obtained from biopsy and diagnosed histopathologically as periapical cyst, periapical abscess, and periapical granuloma at the Department of Dentistry in Hanyang University Hospital were throughly analysed according to the distribution and incidence of age, sex, location, and so on. The following results were obtained : 1.Out of 130 periapical lesions, 88 cases(67.7%) were periapical cysts, 30 cases(23.1%) were periapical abscesses, and 12 cases(9.2%) were periapical granulomas. 2.The periapical lesions occurred most frequently in the third decade, and followed by the fourth, fifth, sixth, and second decade. The periapical cysts occurred most frequently in the third decade(26.1%), the periapical granulomas in the fourth decade(33.3% ) the periapical abscesses in the sixth decade(26.7%). 3.The periapical lesions were more frequent in men than in women with the ratio of men to women of 1.4 : 1. The radio of men to women of periapical cysts was 1.6 : 1, that of periapical granulomas was 0.5 : 1, and that of periapical abscesses was 1.3 : 1. 4.The periapical lesions were more frequent in maxilla than in mandible with the ratio of 1.2 : 1. The ratio of maxilla to mandible of periapical cysts was 1.5 : 1, that of periapical granulomas was 0.5 : 1, that of periapical abscesses was 1 : 1. 5.The most commonly involved location of the periapical lesions was maxillary anterior teeth(40.8%), and followed by mandibular molars, mandibular premolars, and mandibular anterior teeth and maxillary molars. The most frequent location of the periapical cysts was maxillary anterior teeth(48.9%), that of periapical granulomas was mandibular molars(50.0%), that of periapical abscesses was mandibular molars(40.0%).

음이온에 의한 토마토 뿌리조직 마이크로솜 H^+-ATPase 활성 저해

신대섭,조광현,김영기 충북대학교 첨단원예기술개발연구센터 2000 연구보고서 Vol.5 No.-

식물 뿌리세포의원형질막 및 액포막에 위치하는 H+-ATPase들은 세포의 여러 가지 생리활성에 중요한 역할을 수행한다. H+-ATPase의 생리활성 특성을 조사하기 위하여 토마토 뿌리조직으로부터 마이크로솜을 분리하고, H+-ATPase 활성에 미치는 음이온의 효과를 조사하였다. 다양한 종류의 음이온들이 H+-ATPase의 활성을 저해함을 확인하였으며, 이들 중 특히 효소의 저해정도가 다른 citrate와 인산을 선택하여 작용특성을 조사하였다. Citrate에 의한 ATPase 활성저해는 3mM 이상에서 나타났고, 20 mM citrate는 활성을 50-60% 저해하였다. 그러나, citrate,의 저해효과는 MG2+의 농도를 증가시킬수록 감소하여, citrate에 의해 저해된 ATPase 활성은 Mg2+에 의해 회복되는 것으로 나타났다. 즉, 7 mM Mg2+을 첨가하였을 때, citrate에 의한 활성저해는 관측되지 않았고 ATPase 활성은 대조활성과 비슷한 수준으로 회복하였다. 이러한 결과는 citrate가 Mg2+을 chelation함으로써 H+-ATPase의 활성을 저해하기 때문이다. 한편, 인산에 의한 ATPase 활성저해는 3mM 이상의 농도에서 나타났고, 30 mM 인산은 ATPase의 활성을 50% 저해하였다. 인산에 의해서 저해된 ATPase의 활성은 MG2+의 농도증가에 의해 회복되지 않아, 인산에 의한 저해효과는 Mg2+과 무관하였다. H+-ATPases located on plasma and vacuolar membranes play major roles in various cellular physiological processes. In order to investigate the physiological roles of H+-ATPases, microsomes were prepared from tomato roots and the effects of various anions were measured on the activities of H+-ATPases. H+-ATPase was inhibited by various anions. Citrate and phosphate were chosen to investigate detailed inhibitory mechanisms on ATPases since they showed different levels of inhibition. Inhibitory effect of citrate was observed at the concentrations above 3 mM. when 20 mM citrate was added, the H+-ATPase activity was decreased by 50-60%. However, the inhibitory effect of citrate was decreased by increasing the concentration of Mg2+. The citrate-induced inhibited activity was recovered by the addition of Mg2+. Addition of 7 mM Mg2+ completely removed the inhibitory effect of citrate and the activity recovered to the level of the control experiment. These results imply that citrate chelates Mg2+ and thus inhibits H+-ATPases. Meanwhile, the inhibitory effect of phosphate was observed at the concentration above 3 mM and the activity was decreased by 50% in the presence of 30 mM phosphate. Further addition of Mg2+ showed no recovery on the activity. These results imply that the inhibitory effect of phosphate is not dependent upon the concentration of Mg2+. Key words: anion, citrate, phosphate, H+-ATPase, tomato roots Abbreviation: PK, pyruvate kinase; LDH, lactate dehydrogenase; HEPES, N-(2-hydroxyethyl)-piperazine-N'-(2-ethanesulfonic acid); NADH, nicotinamide adenine dinucleotide

한국인 두경부암종 환자에서 Cytochrome P450 1A1,2E1 및 N-acetyltransferase 2 효소의 다형성 분석에 따른 유전적 감수성에 대한 연구

심광섭,김태균,우순섭,이영수,공구 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Individual genetic susceptibilities to cancers may result from several factors including differences in xeno-biotics metabolism to chemical carcinogens, DNA repair, altered oncogenes and suppressor genes, and environmental carcinogen exposures. Among them, genetic polymorphisms of metabolizing enzymes to chemical carcinogens have been recognized as a major important host factors in human cancers. They have two main types of enzymes: the· phase I cytochrome P-450 mediating enzymes (CYPs) and phase Ⅱ conjugating enzymes. The purpose of this study is to determine the frequencies of genotypes of phase I (CYP1A1 and CYP2E1) and phase Ⅱ (NAT2) metabolizing enzymes in healthy control and head and neck cancer patients of Korean and to identify the relative high risk genotypes of these metabolizing enzymes to head and neck cancer in Korean. The author has analyzed 132 head and neck cancer patients and 113 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . The results were as following; 1.The frequencies of genotypes of CYP1A1, CYP2El and NAT2 in healthy control were as following; CYP1A1 exon 7 polymorphism; Ile / Ile: Ile/Val: Va1/Va1 = 59.3%: 36.3%: 4.4% CYP2El Pst I polymorphism, C1/Cl: C1/C2: C2/C2 = 61.1%: 32.1%: 6.2% NAT2 polymorphism; F/F: F/S: S/S = 43.4%: 48.7%: 8.0% 2.In analysis of phase I enzyme, Val/Val genotype in CYP1A1 exon 7 polymorphism and C2/C2 genotype in CYP2El Pst I polymorphism were associated with relative high risks to head and neck cancers (Odds' ratio: 2.09 and 1.37, respectively). 3.Among the genotypes of NAT2 enzyme polymorphism, S/S genotype of NAT2 enzyme had 1.03 times of relative risk to head and neck cancers. 4.In combined genotyping of CYP1A1, CYP2El, and NAT2 enzymes polymorphisms, the patients with Va1/Va1 and Cl/Cl, C2/C2 and fast acetylator, and Va1/Va1 and fast acetylator had higher relative risks than the patients with each baseline of combined genotypes (Odds' ratio: 2.82, 1.98 and 2.1, respectively) . These results suggest the combined genotypes of Va1/Va1 and Cl/Cl , C2/C2 and fast acetylator, and Val/Val and fast acetylator were more susceptible to head and neck cancers in Korean. And genotyping of metabolizing enzymes could be useful for predicting individual susceptibility to head and neck cancer.

상수 고도처리에서 활성탄 재생기술

손성섭,김진오,최광호 嶺南大學校 環境問題硏究所 1999 環境硏究 Vol.18 No.2

요 약 국내 상수도는 취수원의 90%이상을 지표수에 의존하므로 하천오염에 따른 수원의 수질악화가 불가피하며, 또한 생활수준 향상에 따라 먹는물 수질기준의 강화가 예상되므로 상수고도처리의 도입이 절실한 실정이다. 오존처리와 활성탄 여과는 현재 가장 널리 적용되는 상수고도처리법으로서, 특히 활성탄 여과는 냄새, 맛, 색도 및 난분해성 유기물질을 흡착하는데 효과적이므로 국내 20여개 정수장에서 적용하고 있다. 활성탄 여과지에 사용되는 활성탄은 피흡착물에 의해 포화흡착된 후 신탄으로 교체하거나 재생공정을 거쳐 재사용하며, 재생 방법으로는 열재생, 용매추출, 생물학적 산화, 화학적 산화 등이 있으나 활성탄을 다량 사용하는 수처리에는 열재생법이 가장 경제적인 방법으로 평가된다. 열재생은 가열에 의해 흡착 유기물을 제거하는 것으로 건조, 탄화, 부활의 공정으로 진행되며, 재생설비로는 다단로, 로타리킬른, 유동층로, 이동층로 등을 고려할 수 있다. 국내에서의 활성탄 재생설비는 제당공장, 연구용 및 고도폐수처리 등에서 일부 사용되고 있으며 상수고도처리 분야에서는 국내 몇 개 정수장에 적용하여 시공단계에 있지만 수질오염의 심각성 및 시설의 경제성을 고려할 때 그 수요가 점차적으로 증가될 것으로 예상된다.

주변성 법랑모세포종의 증례

송주섭,김경아,고광준 대한구강악안면방사선학회 2006 Imaging Science in Dentistry Vol.36 No.2

Peripheral ameloblastoma is an extremely rare odontogenic soft tissue tumor with histologic characteristics similar to those of the intraosseous ameloblastoma. It appears in the gingiva and oral mucosa. And it usually does not show any bone involvement on radiographs, except for saucer shaped erosion of underlying alveolar bone. Recurrence is considered uncommon. We report a case of peripheral ameloblastoma with bone involvement. Histologically it presented with follicles and nest of tumor cells with palisading pattern. And radiographs showed the typical saucer shaped alveolar bone erosion at the distal area of right mandibular third molar. At 6-month follow-up after operation, no local recurrence was noted.

비균일 열유속하에서 압력강하 불안정성의 요동특성

申光燮,金秉宙 弘益大學校 科學技術硏究所 1993 科學技術硏究論文集 Vol.3 No.-

An experimental study on the oscilatory characteristics of pressure-drop oscillations (PDO) has been carried out in a non-uniformly heated boiling channel using freon-113 as the working fluid. The effects of initial and boundary conditions on PDO are investigated in terms of oscillation period and amplitude. Generally boiling channel under uniform heat flux showed higher amplitude and period of PDO than that of consine-type non-uniform heat flux. The period and amplitude of PDO are increased with the increase in the compressible volume in surge tank, heat input, and with the decrease in the initial flowrate of the fluid supply sectin. However the effects of inlet temperature of working fluid are very similar for both the uniformly and non-uniformly heated channels.

치주인대 세포의 보관방법에 따른 생활력에 관한 연구

현원섭,이긍호,김광철 大韓小兒齒科學會 1994 大韓小兒齒科學會誌 Vol.21 No.1

This study was designed to compare the ability of human periodontal ligament cell viability according to different storage methods, storage time and temperature, and to evaluate the osmolality and pH of the storage media. In the present investigation, media tested for their ability of PDL cell were HBSS, milk, milk contained antibiotics, saliva, ion drink, saline. Time interval was 2 min, 5 min, 10 min, 30 min, 60 min, 120 mi, and storage temperature was 4。C and room temperature. The viability of PDL cells stored for these media was determined by the trypan blue dye exclusion test. Viable (non-stained) and non-viable (stained) cells were counted in a hemocytometer. The pH of storage media were evaluated at 4。C and room temperature. The osmolality of the samples was analysed in an Osmometer, 3D-Ⅱ model. The results were as follows: 1. PDL cell viability was declined in the sequence of DMEM, HBSS, milk contained antibiotics, milk, saline, saliva, ion drink, Distilled water(p<0.01). 2. PDL cell viability was more depended on pH than Osmolality of storage media. 3. PDL cell viability was better at 4。C than room temperature(p<0.01). 4. PDL cell viability was decreased with time(p<0.01).

Hg^2+에 의한 토마토 뿌리조직 마이크로솜 H^+-ATPase의 가역적 저해

신대섭,조광현,김영기 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

토마토 뿌리조직의 마이크로솜 ATPase 활성에 대한 중금속의 효과를 조사하기 위하여 뿌리조직으로부터 마이크로솜을 분리하였고, enzyme-coupled assay를 이용하여 마이크로솜 이온펌프(ATPase)의 활성을 측정하였다. 여러 가지 중금속 이온들 중 Hg2+은 마이크로솜 ATPase 활성을 농도 의존적으로 저해하였으며, Gd3+ 과 Fe3+, La3+, Zn2+ 그리고 Pb2+ 등은 마이크로솜 ATPase의 활성을 현저히 저해하면서 동시에 assay에 사용된 효소를 저해하였다. 그러나, CS+과 BA2+은 마이크로솜 ATPase 활성에 영향을 미치지 않았다. Hg2+은 원형질막과 액포막에 위치하는 H+-ATPase들의 활성을 10 μM 이상의 농도에서 급격히 저해하였고, 1 mM 이상의 농도에서 완전히 저해하였으며, 두 효소들에 대한 활성저해의 Ki 값은 각각 80 , μM, 58 μM로 나타났다. Hg2+에 의해 저해된 ATPase의 활성은 DTT의 농도를 증가시킴에 따라 회복되어, HG2+에 의한 ATPase 활성저해는 가역적임을 확인하였다. 이러한 결과들은 Hg2+이 원형질막과 액포막에 위치한 H+-ATPase들을 비선택적이고 가역적으로 저해함을 보여준다. In order to characterize the effects of heavy metal ions on the microsomal ATPase activities, microsomes were prepared from the roots of tomato plant and the activity of microsomal ATPase was measured by an enzyme-coupled assay. Hg2+ inhibited the activity of microsomal ATPase in a dose-dependent manner, while Gd3+, Fe3+, La3+, Zn2+, and Pb2+ inhibited not only the ATPase activity but also the activities of enzymes used in the assay. However, Cs+ and Ba2+ showed no significant effect. Hg2+ inhibited the activities of both plasma membrane and vacuolar membrane H+-ATPases. In the dose-response to Hg2+, the activities of both microsomal H+-ATPases were severely inhibited at the concentration of Hg2+ above 10μM and were completely inhibited at 1 mM Hg2+. Apparent Ki values of Hg2+ on the inhibitions of plasma membrane and vacuolar membrane H+-ATPases were 80 μM and 58 μM, respectively. The Hg2+-induced inhibitions were reversible since the addition of dithiothreitol completely reversed the inhibitory effects of Hg2+.These results suggest that the inhibitory effects of Hg2+ on both plasma membrane and vacuolar membrane H+-ATPases are nonselective and reversible.

가소제 종류에 따른 아크릴레이트 아이오노머의 기계적 성질에 대한 영향

고광환,김준섭 조선대학교 생산기술연구소 2007 生産技術硏究 Vol.29 No.2

The present study aimed to investigate the effects of additive (DOP, glycerol and 4-DA) on the mechanical properties of acrylate based ionomers neutraized with Na^(+). In experiments, the dynamic mechanical properties of PEA-ionomers containing polar and non-polar additives were compared with those of the PMA and PMMA ionomer. In the case of the PEA-ionomers containing non-polar 4-DA, residing in non-ionic regions of the ionomer, the matrix and cluster Tgs of the ionomer decreased. It was observed that the matrix Tgs of PMA ionomer decreased by the addition of all the additives. However, the two Tgs of PMMA ionomer increased with the addition of all the additives. In PMMA ionomers the partition of the additives took place into both the multiplets and the backbone regions, resulting in an increase in clustering.