난소절제후 Estrogen을 투여한 흰쥐 대동맥에서 Protein Kinase C-α와 Fas 발현의 변화

이왕수,이상엽,안지현,송영빈,김학진,이광재,유재격,김상욱,김태호,김치정,류왕성 중앙대학교 의과대학 의과학연구소 2001 中央醫大誌 Vol.26 No.3

Estrogen is believed to decrease coronary artery disease. Protein kinase C-α(PKCα)appear to be important in signal conduction pathways. Estrogen treatment increases catalytic activity of PKCα,and activation of PKCαcan modulate estrogen receptor levels and responsiveness. And it was reported that activation of PKC can protect cells from apoptosis induced by Fas ligation. But the beneficial effect of estrogen on PKCαwas not clearly demonstrated. The objective of this study was to evaluate the role of PKCαafter ovariectomy and estrogen therapy in rat aorta. The results were summarized as below: 1. The significant pathological changes were not observed in the rat aorta irrelevant to ovariectomy and estrogen therapy. 2. The expression of Fas was decreased in the aorta of estrogen-treated rats than that of ovariectomized rats without estrogen therapy. 3. Increased expression of PKCαwas more marked in the aprta of estrogen-treated rats than that of ovariectomized rat without estrogen therapy. In conclusion,PKCαmay be important in signal conduation pathway on the effect of estrogen. Activation of PKCαby estrogen reduced Fas expression, suggesting that PKCαactivation may play a role in protection against atherasclerosis. Further studies are needed to elucidate the role of PKCαafter estrogen therapy.

급성하벽심근경색에서 전흉부유도 ST절 하강의 의의

정병천,배호상,곽동훈,김영태,배용학,황종현,임현주,류재근,조용근,채성철,전재은,박의현 慶北大學校 醫科大學 1997 慶北醫大誌 Vol.38 No.2

목적 : 급성하벽심근경색증 환자에서 심전도상 흔히 동반되는 전흉부유도 ST절 하강의 의의를 알아보았다. 방법 : 심근경색후 24시간에서 48시간에 얻은 심전도를 기준으로 52명의 급성하벽심근경색증 환자를 전흉부유도에서 ST절의 하강이 있는 군과 없는 군으로 나누었으며 심도자, 관상동맥조영술, 심초음파도 및 방사성 핵종을 이용한 좌심구혈율 측정 등을 시행하였다. 결과 : 1) 좌심실확장기말압은 전흉부유도에서 ST절의 하강이 있는 경우에서 16.9±5.33mmHg로 없는 경우의 11.0±4.02mmHg보다 유의하게 높았다(p<0.05). 그러나 운동량 및 좌심구혈을은 흥부 유도상 ST절의 하강이 있는 경우 7.6±3.0 METs 및 53.9±9.1%로 없는 경우의 9.1±2.7 METs 및 54.6±9.5%보다 낮았으나 통계적 유의성은 없었다(p=0.180, p=0.823). 2) 관상동맥조영술상 전흉부 ST절의 하강이 동반된 군에서는 유의한 좌전하행지나 좌회선지의 협착이 있는 경우가 60.9% 및 57.9%로 ST절의 하강이 없는 군에서의 34.5% 및 7.7%와 비교해 좌관상동맥에 협착이 동반된 경우가 많았다(p=0.050, p=0.001). 3) 관상동맥조영술상 세 혈관질환의 빈도는 흉부유도상 ST절의 하강을 보인 23예에서 10예로 ST절 하강이 없었던 29예중 2예에 비하여 유의하게 많았고(P=0.007), 단 혈관질환의 빈도는 ST절의 하강을 보인 군에서 23예중 5예로 ST절 하강이 없었던 29예중 17예에 비하여 유의하게 적었다(P=0.002). 두 혈관질환의 경우에는 각각 8예와 10예로 양군간에 유의한 차이가 없었다. 4) 흉부유도상 ST절 하강의 빈도는 단 혈관질환 22예 중 5예에서, 두 혈관질환 18예중 8예, 세 혈관질환 12예 중 10예에서 관찰되어 협착이 있는 관상동맥의 수가 많을수록 ST절의 하강이 나타나는 빈도가 많음을 보였다(p=0.00308). 그러나 흉부유도상 나타난 ST절의 하강을 모두 합하여 구한 ST절 하강의 합은 협착이 있는 관상동맥의 수와 통계적인 유의성이 없었다. 5) 심초음파도상 좌심실 하벽의 벽운동은 ST절 하강을 보인 군에서는 akinesia가 83.3%, hypokinesia가 11.1%, 정상 벽운동이 5.6%로, ST절 하강이 없었던 군에서의 dyskinesia가 3.6%, akinesia가 39.3%, hypokinesia가 25.0%, 정상 벽운동이 32.1%와 비교하여 ST절의 하강이 있는 군에서 유의하게 벽운동의 장애가 빈번함을 보였다(p=0.03891). 그러나 좌심실전벽, 측벽 및 중격부위에서는 유의한 벽운동의 차이가 없었다. 결론 : 급성하벽심근경색증 환자의 전흉부유도에서 ST절의 하강이 동반되는 경우 다혈관 질환의 가능성이 높으며 좌심실확장기말압이 상승되었고 운동능력은 감소되어 있었다. 또한 심초음파도상 좌심실하벽의 벽운동장애도 심하였다. 따라서 전흉부 ST절의 하강을 동반한 급성하벽심근경색증 환자는 전흉부유도에 ST절 하강이 없는 경우보다 예후가 불량할 것으로 생각되며, 보다 적극적인 치료가 필요할 것으로 생각된다. The purpose of this study was to determine the significance of precordial ST segment depression appearing during acute inferior myocardial infarction and to assess the correlation of that with angiographic finding. 52 patients were allocated into two groups based on the existence of precordial ST segment depression : 23 patients with ST segment depression and 29 patients without ST segment depression. The extent of coronary artery disease as well as the prevalence of significant stenosis (≥70%) in left anterior descending artery were more frequent in patients showing precordial ST segment depression than in patients not showing them (p=0.050) and the result were similar in cases of left circumplex artery (p=0.001). On echocrdiographic examination of left ventricular (LV) wall motion, it showed more severely decreased inferior LV wall motion in patients showing precordial ST segment depression than in patients not showing them (p=0.03891) even though the other segment of LV wall motion didn't show the statistical difference between both groups. The other cardiac parameters such as LVEDP, exercise amount (METs) and ejection fraction were tend to showing bad profile in patients with precordial ST segment depression. From the above results, we could deduce that patients with precordial ST segment depression have relatively large infarction or concomitant left coronary artery disease, so they need more caution and intensive theraphy.

Immunohistomorphometric Analysis of Transplanted Umbilical Cord Blood-Derived Mesenchymal Stem Cells and The Resulting Anti-Inflammatory Effects on Nerve Regeneration of Injured Canine Spinal Cord

( Hak Hyun Ryu ),( Ye Eun Byeon ),( Sung Su Park ),( Byung Jea Kang ),( Min Soo Seo ),( Sang Bum Park ),( Wan Hee Kim ),( Kyung Sun Kang ),( Oh Kyeong Kweon ) 한국조직공학·재생의학회 2011 조직공학과 재생의학 Vol.8 No.2

In order to investigate the beneficial mechanism of transplanted canine umbilical cord blood-derived mesenchymal stem cells (UC-MSCs) on spinal cord injury (SCI), we evaluated recovery in a canine SCI model by examining neurological function and nerve regeneration. Recovery was assessed by clinical observation and by examining regenerated nerve cells by immuno-histomorphometric analysis and by assessing anti-inflammation by measuring mRNA expression of inflammation-related cytokines. Extradural compression of the spinal cord in 21 dogs (4.6±0.4 kg, ~2-3 yrs) was performed using a balloon catheter for 12 hours. All of the dogs showed hind limb paralysis after compression. Functional recovery of the hind limbs was evaluated by the Olby score and Revised modified tarlov scale. Experimental dogs were divided into cUCB and control groups. In the cUCB group (n=12), UC-MSCs were infected with a lentivirus-vector labeled GFP gene and injected into the SCI site. In the control group (n=9), only PBS was injected into the SCI site. Seven dogs (control = 3, cUCB = 4) were euthanized and their injured spinal cords were collected at 1, 4 and 8 weeks after transplantation. Nerve regeneration was assessed on longitudinal sections at the epicenter. After transplantation of UC-MSCs, functional improvement up to 5 points of the Olby score and 4 points of Revised modified tarlov scale were observed. Compared to the control group, immuno-histomorphometrical analyses showed that cells labeled with GFAP were significantly reduced and cells labeled with Tuj1 and NF160 were increased in the cUCB group (p < 0.05). In western blot analysis, total gliosis in the cUCB group was reduced by 35% compared to controls and surviving nerve cells in the SCI lesion were increased by more than 50 % compared to controls at 8 weeks after transplantation. However, it must be noted that a small number of nerve cells were derived from the transplanted UC-MSCs. In addition, the expression of COX2, IL1, IL6, TNF and TGF-β, in the cUCB group were down regulated. These findings suggest that improvements of neurological function seen after transplantation of UC-MSCs into injured spinal cord might be due primarily to reduced gliosis by anti-inflammation, increased survival of endogenous nerve cells and enhanced function of survived endogenous nerve cells by engrafted cells.

Comparison of Mesenchymal Stem Cells Derived from Fat, Bone Marrow, Wharton^|^rsquo;s Jelly, and Umbilical Cord Blood for Treating Spinal Cord Injuries in Dogs

RYU, Hak-Hyun,KANG, Byung-Jae,PARK, Sung-Su,KIM, Yongsun,SUNG, Gyu-Jin,WOO, Heung-Myong,KIM, Wan Hee,KWEON, Oh-Kyeong Japanese Society of Veterinary Science 2012 The Journal of veterinary medical science Vol.74 No.12

A Sustainable Tourism Study in Underdeveloped Areas Using Big Data Analysis Techniques

Hyun-Seok Kim,Sang-Hak Lee,Gi-Hwan Ryu The Institute of Internet 2024 International Journal of Internet, Broadcasting an Vol.16 No.2

We Design The problem of underdeveloped areas is emerging as a social problem. Industrialization drove the population to the cities, creating underdeveloped areas. Underdeveloped areas are causing social problems such as population decline and aging. It is necessary to study the continuous tourism development of underdeveloped areas through development and improvement projects. Using social media big data to investigate keywords in underdeveloped areas and see the connection between keywords. The purpose of this study was to conduct core research divided by type and to investigate the keywords of tourism in underdeveloped areas through concor analysis of underdeveloped areas. As a result of the study, keywords were connected for each type of redevelopment, regional development, regional economy, and underdeveloped areas. Through this, the keywords for sustainable tourism in underdeveloped areas were identified. It is hoped that this study will develop sustainable tourism for the keywords of underdeveloped areas.

초등학교 아동들의 비만 발생에 영향을 미치는 요인 : 2년 추적조사 연구 2 Years Follow-up Study

류소연,박종,이철갑,박상기,배화연,안현옥,박영봉,문경래,양은석,노영일 대한비만학회 1998 The Korean journal of obesity Vol.7 No.2

This prospective study was performed to find the associated factors of obesity in primary school children in Kwangju. We studied obesity incidence over 2 years among 4th grade primary school children, In 1994, base line study about 1,344 children of 4th grade was performed and in 2 years later, we reinvestigated health examination and questionnaire about several variables in 922. The 797 children included in the analysis had non-obese(normal and overweight) at baseline in 1994. This results were as follows; l. In 2 years later, 3.5% of normal and 15% of overweight children were to the obese. 2. The body mass index and obesity in one or both parents, sex, overweight at baseline, regular exercise and meat preference were statistically significant factors in simple analysis. 3. In multiple logistic regression analysis, the odds ratios associated with obesity were 0.41 (95% CI: 0.20-0.82) for female, 0.48(95% CI: 0.25-0.92) for doing regular exercise, 4,95(95% CI: 2.55-9.59) for overweight at baseline, and 3.22(95% CI: 1.11-9.34) for obesity in both parents. In conclusion, obesity incidence in primary school children was associated with physical characteristics, genetic factors and social factors like a life style. It is suggested that obesity management and appropriate regular exercise in family unit should be required to prevent obesity in children.

Increased Cordycepin Production by the Inhibition of Adenosine Deaminase Activity

Hak Hyun Lee,Ayman Turk,Solip Lee,Sang Won Yeon,Se Hwan Ryu,Sung Min Ko,Beom Seok Kim,Bang Yeon Hwang,Mi Kyeong Lee 한국버섯학회 2023 버섯 Vol.27 No.1

Activin A/BMP2 Chimera (AB204) Exhibits Better Spinal Bone Fusion Properties than rhBMP2

Ryu, Dalsung,Yoon, Byung-Hak,Oh, Chang-Hyun,Kim, Moon-Hang,Kim, Ji-Yong,Yoon, Seung Hwan,Choe, Senyon The Korean Neurosurgical Society 2018 Journal of Korean neurosurgical society Vol.61 No.6

Objective : To compare the spinal bone fusion properties of activin A/BMP2 chimera (AB204) with recombinant human bone morphogenetic protein (rhBMP2) using a rat posterolateral spinal fusion model. Methods : The study was designed to compare the effects and property at different dosages of AB204 and rhBMP2 on spinal bone fusion. Sixty-one male Sprague-Dawley rats underwent posterolateral lumbar spinal fusion using one of nine treatments during the study, that is, sham; osteon only; $3.0{\mu}g$, $6.0{\mu}g$, or $10.0{\mu}g$ of rhBMP2 with osteon; and $1.0{\mu}g$, $3.0{\mu}g$, $6.0{\mu}g$, or $10.0{\mu}g$ of AB204 with osteon. The effects and property on spinal bone fusion was calculated at 4 and 8 weeks after treatment using the scores of physical palpation, simple radiograph, micro-computed tomography, and immunohistochemistry. Results : Bone fusion scores were significantly higher for $10.0{\mu}g$ AB204 and $10.0{\mu}g$ rhBMP2 than for osteon only or $1.0{\mu}g$ AB204. AB204 exhibited more prolonged osteoblastic activity than rhBMP2. Bone fusion properties of AB204 were similar with the properties of rhBMP2 at doses of 6.0 and $10.0{\mu}g$, but, the properties of AB204 at doses of $3.0{\mu}g$ exhibited better than the properties of rhBMP2 at doses of $3.0{\mu}g$. Conclusion : AB204 chimeras could to be more potent for treating spinal bone fusion than rhBMP2 substitutes with increased osteoblastic activity for over a longer period.

Health Behaviors, Periodontal Conditions, and Periodontal Pathogens in Spontaneous Preterm Birth: A Case-Control Study in Korea

Ryu, Jae-In,Oh, KyungJoon,Yang, HyeJin,Choi, Bong-Kyu,Ha, Jung-Eun,Jin, Bo-Hyoung,Kim, Hyun-Duck,Bae, Kwang-Hak Wiley (John WileySons) 2010 Journal of periodontology Vol.81 No.6

Comparison and Quantitative Analysis of Various Oyster Mushrooms Species

Hak Hyun Lee,Solip Lee,Kyoung Tae Lee,Rhim Ryoo,Youngki Park,Se Hwan Ryu,Bang Yeon Hwang,Mi Kyeong Lee 한국버섯학회 2024 버섯 Vol.28 No.1