http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Antiviral activity of KR-23502 targeting nuclear export of influenza B virus ribonucleoproteins
Jang, Y.,Lee, H.W.,Shin, J.S.,Go, Y.Y.,Kim, C.,Shin, D.,Malpani, Y.,Han, S.B.,Jung, Y.S.,Kim, M. Elsevier/North-Holland 2016 ANTIVIRAL RESEARCH Vol.134 No.-
<P>The spiro compound 5,6-dimethyl-3H,3'H-spiro(benzofuran-2,1'-isobenzofuran)-3,3'-dione (KR-23502) has antiviral activity against influenza A and more potently B viruses. The aim of this study is to elucidate its mechanism of action. Subcellular localization and time-course expression of influenza B viral proteins, nucleoprotein (NP) and matrix protein 1 (M1), showed that KR-23502 reduced their amounts within 5 h post-infection. Early steps of virus life cycle, including virus entry, nuclear localization of NP and viral RNA-dependent RNA replication, were not affected by KR-23502. Instead it interrupted a later event corresponding to nuclear export of NP and M1 proteins. Delivery of viral ribonucleoprotein (vRNP)-M1 complex has been known to be mediated by the viral nuclear export protein (NEP) through interaction with cellular chromosomal maintenance 1 (CRM1) protein. In this study, we experimentally demonstrated that the compound targets the nuclear export of vRNP. Moreover, a single mutation (aspartate to glycine) at amino acid position 54 in M1 [M1(D54G)] was detected after 18 passages in the presence of KR-23502 with a 2-fold increase in 50% effective concentration indicating that this compound has a relatively high genetic barrier to resistance. Interestingly, it was observed that proteasome-mediated degradation of M1 (D54G) was attenuated by KR-23502. In conclusion, we suggest that KR-23502 shows its anti-influenza activity by downregulating NEP/CRM1-mediated nuclear export of influenza vRNP and M1. KR-23502 provides a core chemical skeleton for further structure-based design of novel antivirals against influenza viruses. (C) 2016 Elsevier B.V. All rights reserved.</P>
Microporous ZIF-7 membranes prepared by in-situ growth method for hydrogen separation
Go, Y.,Lee, J.H.,Shamsudin, I.K.,Kim, J.,Othman, M.R. Pergamon Press ; Elsevier Science Ltd 2016 International journal of hydrogen energy Vol.41 No.24
<P>The H-2/CO2 selectivity across ZIF-7 membrane prepared by in-situ growth method at synthesis temperature and time of 105 degrees C and 2 h, respectively, was the highest at 15.98, nearly four times higher than H-2/CO2 Knudsen separation factor of 4.7. ZIF-7 membranes prepared from in-situ growth method also surprisingly performed better than ZIF-7 membranes prepared by other innovative techniques such as electro-spray deposition and secondary growth methods that achieved selectivity of 9.59 and 4.7, respectively. Despite lower selectivity performance than the numerically predicted results (the latter fall within the commercially attractive region with H-2/CO2 selectivity of 200 and permeability of 2000 barrer), the micro-porous ZIF-7 membrane prepared in this work demonstrated higher H-2 permeability of 3770 barrer. Performance comparison between various inorganic membranes, including ZIF-7 and ZIF-8 membranes (that are highly potential for commercial application with H-2/CO2 selectivity of 20.29 and permeability of 35541 barrer) was made and a new upper boundary for inorganic membranes was also constructed and reported. (C) 2015 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.</P>
한국 소재 대학교에 재학 중인 재외국민대학생의 문화지능과 학습참여도가 우울에 미치는 영향
강보미,강유빈,고미영,김찬송,남의정,박소은,박정연,백도윤,이혜린,한보혜,신주현,손지희 이화여자대학교 간호과학대학 2019 이화간호학회지 Vol.- No.53
Purpose: This study aims to identify the impact that cultural intelligence and learning engagement of Korean overseas students in Korea have in depression, also, to propose direction for further research, and to suggest a nursing intervention program that aims to the reduction of depression in overseas students. Methods:. The selected participants of the present study were Korean Overseas University Students enrolled in a special admission for overseas students. Data were obtained through the use of SPSS Window 25 and analyzed using descriptive statistics, t-test, ANOVA, Scheffe test, Pearsons correlation coefficient, and multiple linear regression analysis. Results: Depression in Korean overseas university students is influenced by the education system(ß=-.193, p=.046) and continent of residency(ß=0.197, p=.03). It is necessary to take into account the differences of education systems as well as the cultural differences from where they come from for the purpose of reducing depression rates in overseas Koreans. Conclusion: The results of this study show that depression in Korean overseas students is related to the differences in continents of residency and the education systems. Consequently, the development and implementation of a nursing intervention program considering both factors, the continent and education system, has become a necessity that relates to the reduction of depression in Korean overseas students.
Development of a microfluidic perfusion 3D cell culture system
Park, D H,Jeon, H J,Kim, M J,Nguyen, X D,Morten, K,Go, J S IOP 2018 Journal of micromechanics and microengineering Vol.28 No.4
<P>Recently, 3-dimensional <I>in vitro</I> cell cultures have gained much attention in biomedical sciences because of the closer relevance between <I>in vitro</I> cell cultures and <I>in vivo</I> environments. This paper presents a microfluidic perfusion 3D cell culture system with consistent control of long-term culture conditions to mimic an <I>in vivo</I> microenvironment. It consists of two sudden expansion reservoirs to trap incoming air bubbles, gradient generators to provide a linear concentration, and microchannel mixers. Specifically, the air bubbles disturb a flow in the microfluidic channel resulting in the instability of the perfusion cell culture conditions. For long-term stable operation, the sudden expansion reservoir is designed to trap air bubbles by using buoyancy before they enter the culture system. The performance of the developed microfluidic perfusion 3D cell culture system was examined experimentally and compared with analytical results. Finally, it was applied to test the cytotoxicity of cells infected with Ewing’s sarcoma. Cell death was observed for different concentrations of H<SUB>2</SUB>O<SUB>2</SUB>. For future work, the developed microfluidic perfusion 3D cell culture system can be used to examine the behavior of cells treated with various drugs and concentrations for high-throughput drug screening.</P>
Nam, B.H.,Seo, J.K.,Go, H.J.,Lee, M.J.,Kim, Y.O.,Kim, D.G.,Lee, S.J.,Park, N.G. Academic Press 2012 Fish & Shellfish Immunology Vol.33 No.1
An approximately 21 kDa antimicrobial protein was purified from an acidified testis extract of olive flounder, Paralichthys olivaceus, by ion-exchange and C<SUB>18</SUB> reversed-phase HPLC. A comparison of the N-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone H1 molecules; thus, it was designated flounder histone H1-like protein (fH1LP). fH1LP showed potent antimicrobial activity against Gram-positive bacteria, including Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 2.8-30.0 μg/ml), Gram-negative bacteria, including Aeromonas hydrophila, Escherichia coli D31, Vibrio parahaemolyticus (MECs, 1.4-12.0 μg/ml), and Candida albicans (MEC, 2.0 μg/ml). cDNA cloning and tissue distribution studies of fH1LP indicated that it is constitutively expressed in testis and ovary. The fH1LP expression level was significantly dependent on developmental stage, and decreased dramatically after hatching. However, lipopolysaccharide stimulation did not induce fH1LP mRNA in other immune organs, including the kidney and spleen. These results suggest that fH1LP plays an important role in innate immunity in fish during reproduction, including mating, fertilization, and hatching.
Zhu, L. L.,Park, Y. J.,Gan, L.,Go, S. I.,Kim, H. N.,Kim, J. M.,Ko, J. W. Chapman and Hall 2017 Journal of materials science Materials in electron Vol. No.
<P>Highly transparent Y2O3 ceramics doped with different Zr concentrations were successfully fabricated by vacuum pre-sintering at temperatures ranging from 1600 to 1800 degrees C combined with a subsequent hot-isostatic pressing (HIP) treatment using commercial powders as the starting materials. All of the 1 mol% Zr-doped Y2O3 ceramics exhibit very good optical quality. The sample with the highest transparency level was realized by vacuum sintering at 1650 degrees C for 4 h followed by a post-HIP treatment at 1450 degrees C lasting 5 h. It has a fine microstructure and the grain size is 1.48 mu m. Furthermore, the in-line transmittance reaches 83.3% at 1100 nm (1.2 mm thickness). It was found that a relatively low vacuum sintering temperature (1650 degrees C) and relatively low Zr doping concentration (1 mol%) are more appropriate to achieve optimally transparent Y2O3 ceramics with a subsequent HIP treatment.</P>