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      • Research on Overload Monitoring and Alarming based on APP

        Feng Pengfei,Jin Huiqing,Gu Jiangyang,Han Jian 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.11

        As the acceleration of urban construction, the dump heavies(building soil-transporting vehicle) shuttle back and forth in urban road. For the sake of profits, the crazy overload transportation makes the vehicles lose braking control and steering ability in emergency state. The common occurrence of traffic disasters brings huge hidden trouble of urban traffic safety. Its harm is self-evident, causing serious damage to infrastructure, to threaten the safety of life and property of the people, to disrupt the market economic order, but also cause environmental pollution. In recent years, the traffic management departments and urban management departments jointly monitor the discharging heavy trucks in the forms of surveillance and reporting by the masses, installing camera and monitor, self-discipline and self-supervision of investors, etc. These forms are limited to the forms and surface, so they cannot effectively stop the overload of self-discharging trucks. How to better and accurately monitor and stop the over-load? The paper intends to achieve intelligent monitoring and alarming on the overload of dump heavy through internet + public transportation monitoring. First the controller is selected and the weighting sensing system is designed through selecting the weighting sensor and determining the installation position of weighting sensor. Then the construction of APP overload monitoring is finished by the three steps including constructing dual-core system, generating database and designing APP display module. Finally after finishing the design of intelligent overload detection system, the paper conducts experiments to validate its effectiveness. The results show that when considering no electromagnetic, the overload equipment and APP software system could receive the overload information timely and reliably.

      • KCI등재

        Numerical analysis on the performance of an SCR monolith reactor

        Pengfei Feng,Ding Chen,Yucheng Cao,Yaotong Chen 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.4

        An SCR catalyst is used as an example to investigate the effects of the washcoat diffusion limitation on the performance of monolith reactors. One-dimensional model and three-dimensional CFD model with and without washcoat diffusion limitation were established. The washcoat diffusion was modeled by using an effective diffusivity in the washcoat region (CFD modeling) and the internal mass transfer coefficient between the interface and the interior of the washcoat (One-dimensional modeling). The results show that numerical models with washcoat diffusion limitation give more accurate NO concentration prediction than the numerical models without washcoat diffusion limitation. Using the ratios of internal mass transfer resistance and reaction resistance to estimate the washcoat diffusion limitation, the correlation between the temperature and the washcoat limitation is discussed. Detailed comparisons of the 1-D model and CFD model are conducted.

      • KCI등재

        Characterization and expression analysis of inositolphosphorylceramide synthase family genes in rice (Oryza sativa L.)

        Pengfei Liao,Jinqiu Huang,Puguo Tong,Wang Nie,Xin Yan,Yingmei Feng,Hui Peng,Xiaojue Peng,Shao-Bo Li 한국유전학회 2017 Genes & Genomics Vol.39 No.5

        Genes encoding inositolphosphorylceramide synthase (IPCS), which play essential roles in sphingolipid biosynthesis, have been known to be widely distributed in plants. However, reports on identification, expression, and function analysis of plant IPCS family genes are very limited. In this study, a total of three OsIPCS genes were identified in the rice database, and their full-length coding regions were amplified by RT-PCR. These genes had 12–13 exons, and they encoded proteins of 313–326 amino acids with molecular mass of 35.8–37.6 kDa and isoelectric point of 10.99–12.61. Importantly, two typical and conserved motifs (D3 and D4) in plants were found in all the three putative OsIPCS proteins, indicating high similarity of OsIPCSs to the other plant IPCS proteins, which were confirmed by phylogenetic analysis. The transcripts of OsIPCS genes appeared in different rice organs including seedling roots, stems and leaves, and young panicles, but each gene showed a unique organ-specific expression pattern. Meanwhile, we found that all the three OsIPCS genes showed responses to stresses like drought, cold and salt, but their expression patterns were also to some extent different under stress treatments. Especially, under cold stress, OsIPCS1 and OsIPCS2 were significantly up-regulated in roots and stems but down-regulated in leaves, while OsIPCS3 was significantly up-regulated in all the measured organs. Actually, putative abiotic-stress regulatory elements were identified in the promoters of these genes. Our data suggest that OsIPCS genes should play important roles during rice growth and adversity adaptation.

      • KCI등재

        Preparation and Catalytic Performance of Amidoximated Polyacrylonitrile Bimodal Nanofiber Iron Complexes

        Fu Li,Pengfei Fei,Yongchun Dong,Man Zhang,Yu Feng,Shuqiang Liu,Lu Jia,Hulin Zhang,Zhen Ni 한국섬유공학회 2022 Fibers and polymers Vol.23 No.5

        To balance the specific surface area and porous structure of nanofibrous heterogeneous Fenton catalyst for therapid transfer of dye molecules during dye-containing wastewater treatment, a polyacrylonitrile nanofiber with bimodaldiameter distribution (n-PAN-D) was prepared and amidoximated, followed by coordinating with Fe3+ ion (Fe-AO-n-PAND). The modification and coordination process hardly changed the fiber morphology and bimodal diameter distribution ofnanofiber membrane. The amidoximated degree of n-PAN-D increased with the increase of the diameter difference, and thecoordination ability of amidoximated n-PAN-D decreased with increasing the diameter difference. Fe-AO-n-PAN-D hadbetter catalytic activity than those with similar thick or thin nanofiber diameters due to the rapid mass transfer of dyemolecules in the catalyst. The possible oxidation and degradation pathway of Reactive Red 195 in the Fe-AO-n-PAN-D/H2O2system was proposed. And the series of reactions may not gradually occur because of the non-selective oxidation of ·OH. Thegood reusability of Fe-AO-n-PAN-D made it potential carrier for heterogeneous Fenton catalyst in wastewater treatment.

      • KCI등재

        IRE1α protects against osteoarthritis by regulating progranulin-dependent XBP1 splicing and collagen homeostasis

        Liang Li,Zhang Fengmei,Feng Naibo,Kuang Biao,Fan Mengtian,Chen Cheng,Pan Yiming,Zhou Pengfei,Geng Nana,Li Xingyue,Xian Menglin,Deng Lin,Li Xiaoli,Kuang Liang,Luo Fengtao,Tan Qiaoyan,Xie Yangli,Guo Fen 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

        Osteoarthritis (OA) is a full-joint, multifactorial, degenerative and inflammatory disease that seriously affects the quality of life of patients due to its disabling and pain-causing properties. ER stress has been reported to be closely related to the progression of OA. The inositol-requiring enzyme 1α/X-box-binding protein-1 spliced (IRE1α/XBP1s) pathway, which is highly expressed in the chondrocytes of OA patients, promotes the degradation and refolding of abnormal proteins during ER stress and maintains the stability of the ER environment of chondrocytes, but its function and the underlying mechanisms of how it contributes to the progression of OA remain unclear. This study investigates the role of IRE1α/ERN1 in OA. Specific deficiency of ERN1 in chondrocytes spontaneously resulted in OA-like cartilage destruction and accelerated OA progression in a surgically induced arthritis model. Local delivery of AdERN1 relieved degradation of the cartilage matrix and prevented OA development in an ACLT-mediated model. Mechanistically, progranulin (PGRN), an intracellular chaperone, binds to IRE1α, promoting its phosphorylation and splicing of XBP1u to generate XBP1s. XBP1s protects articular cartilage through TNF-α/ERK1/2 signaling and further maintains collagen homeostasis by regulating type II collagen expression. The chondroprotective effect of IRE1α/ERN1 is dependent on PGRN and XBP1s splicing. ERN1 deficiency accelerated cartilage degeneration in OA by reducing PGRN expression and XBP1s splicing, subsequently decreasing collagen II expression and triggering collagen structural abnormalities and an imbalance in collagen homeostasis. This study provides new insights into OA pathogenesis and the UPR and suggests that IRE1α/ERN1 may serve as a potential target for the treatment of joint degenerative diseases, including OA.

      • The Monocot-Specific Receptor-like Kinase SDS2 Controls Cell Death and Immunity in Rice

        Fan, Jiangbo,Bai, Pengfei,Ning, Yuese,Wang, Jiyang,Shi, Xuetao,Xiong, Yehui,Zhang, Kai,He, Feng,Zhang, Chongyang,Wang, Ruyi,Meng, Xiangzong,Zhou, Jinggeng,Wang, Mo,Shirsekar, Gautam,Park, Chan Ho,Bell Elsevier 2018 Cell host & microbe Vol.23 No.4

        <P><B>Summary</B></P> <P>Programmed cell death (PCD) plays critical roles in plant immunity but must be regulated to prevent excessive damage. The E3 ubiquitin ligase SPL11 negatively regulates PCD and immunity in plants. We show that <I>S</I>PL11 cell-<I>d</I>eath <I>s</I>uppressor <I>2</I> (SDS2), an S-domain receptor-like kinase, positively regulates PCD and immunity in rice by engaging and regulating SPL11 and related kinases controlling defense responses. An <I>sds2</I> mutant shows reduced immune responses and enhanced susceptibility to the blast fungus <I>Magnaporthe oryzae</I>. Conversely, <I>SDS2</I> over-expression induces constitutive PCD accompanied by elevated immune responses and enhanced resistance to <I>M. oryzae</I>. SDS2 interacts with and phosphorylates SPL11, which in turn ubiquitinates SDS2, leading to its degradation. In addition, SDS2 interacts with related receptor-like cytoplasmic kinases, OsRLCK118/176, that positively regulate immunity by phosphorylating the NADPH oxidase OsRbohB to stimulate ROS production. Thus, a plasma membrane-resident protein complex consisting of SDS2, SPL11, and OsRLCK118/176 controls PCD and immunity in rice.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The RLK SDS2 positively regulates plant cell death and immunity in rice </LI> <LI> SDS2 phosphorylates E3 ligase SPL11, which in turn ubiquitinates SDS2 for degradation </LI> <LI> SDS2 phosphorylates receptor-like cytoplasmic kinases RLCK118 </LI> <LI> RLCK118 interacts with and phosphorylates the NADPH oxidase OsRbohB </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

      • KCI등재

        Hot Deformation Behaviors and Process Parameters Optimization of Low-Density High-Strength Fe–Mn–Al–C Alloy Steel

        Peng Wan,Huixiang Yu,Feng Li,Pengfei Gao,Lei Zhang,Zhengzhi Zhao 대한금속·재료학회 2022 METALS AND MATERIALS International Vol.28 No.10

        The hot deformation behavior of low-density high-strength Fe–Mn–Al–C alloy steel at T = 900-1150 °C and ̇ = 0.01-10 s−1was studied by the Gleeble-3500 thermo-mechanical simulator. The rheological stress curve characteristics of the steel wereanalyzed through experimental data, and a physical constitutive model considering strain coupling was established. At thesame time, the finite element software DEFORM was used to calculate the critical damage value of the steel, and the influenceof T and ̇ on the maximum damage value was considered. By introducing the dimensionless parameter Zener–Hollomon, thecritical damage model was established. Finally, the workability of the steel was evaluated by using the intuitive processingmap technology. The results indicated that Fe–Mn–Al–C alloy steel is a positive strain rate-sensitive and a negative temperature-sensitive material, and the constitutive model considering physical parameters can well predict the rheological stress ofthe steel during hot deformation (R = 0.997). The critical damage factor of Fe–Mn–Al–C alloy steel varies with the changeof T and ̇ , and the range is 0.359-0.535. At the same time, the critical damage factor is more sensitive to ̇ . At a constantT, the damage factor decreases with the increase of ̇ . Based on the Prasad instability criterion, the dynamic material modelprocessing map and the microstructure verification after thermal compression, the rheological instability characteristics ofthe steel are mainly mechanical instability and local plastic flow, and the stable deformation area is mainly characterized bydynamic recrystallization. The optimal hot working process window of the steel is 975-1050 °C/0.01-0.032 s−1.

      • KCI등재

        Effect of laser beam incidence angle on cladding morphology in laser cladding process

        Guan Zhang,Wenlei Sun,Dongmei Zhao,Pengfei Fan,Feng Guo,Yong Huang,Pengfei Li 대한기계학회 2020 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.34 No.4

        The change of angle θ between laser cladding powder plane and substrate plane will lead to changes in cladding layer's geometric morphology. Therefore, we established a quantitative numerical prediction model for cladding layer geometry. In this model, we consider the variation of θ, the laser energy attenuation rate and the temperature rise of the powder particles. At the same time, the simulation results were verified by experiments. The results show that when θ is in the range of 50°~90°, the initial temperature is 298 K, the scanning speed is 3.75 mm/s, and the laser spot diameter is 4.5 mm, the Fe#1 powder cladding can achieve better forming effect on Q235. In general, with the decrease of θ, the height of the cladding layer decreases and the width of the layer increases. However, when θ is less than 50°, the quality of the formed morphology significantly deteriorated. The experimental results are in good agreement with the simulation results, which verifies the validity and reliability of the model. This work provides a theoretical reference for further understanding the relationship between the laser cladding morphology and the incident angle.

      • KCI등재

        Modeling of the polycrystalline cutting of austenitic stainless steel based on dislocation density theory and study of burr formation mechanism

        Jiaxin Wen,Lin He,Tao Zhou,Pengfei Tian,Tian Zhou,Zhiguo Feng 대한기계학회 2023 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.37 No.6

        The presence of burrs in mechanical processing can negatively affect surface integrity and dimensional accuracy and even lead to part scrapping. Grain size also has a significant impact on material properties and subsequently generate different processing effects. However, only few studies have explored the effect of grain size on machined surface integrity and burr formation. To fill this gap, this study develops a model based on dislocation density and incorporates this model into ABAQUS using subroutines to investigate polycrystalline cutting modeling and the burr formation mechanism. In addition, the plastic flow and deformation process of grains are observed using a newly developed 2D polycrystalline model, which is later compared with traditional Johnson-Cook constitutive models. The simulated cutting force value, chip morphology, and experimental results are also compared to preliminarily validate the feasibility of the developed model. The experimental results remain consistent across different simulated cutting speeds and depths. The height and width of the exit burr slightly decrease along with increasing cutting speed. Meanwhile, increasing the cutting depth significantly increases the burr width and height. The lateral burrs observed in the experiment can also be reproduced using a 3D polycrystalline model. The lateral burr size increases along with cutting depth and speed. The effect of grain size on cutting force and burr formation is eventually explored, and results show that increasing the grain size reduces the cutting force but increases the burr size. This study provides a new concept for burr control and surface integrity improvement.

      • KCI등재

        Lactate potentiates angiogenesis and neurogenesis in experimental intracerebral hemorrhage

        Jing Zhou,Tao Liu,Hao Guo,Hanjin Cui,Pengfei Li,Dandan Feng,En Hu,Qing Huang,Ali Yang,Jun Zhou,Jiekun Luo,Tao Tang,Yang Wang 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

        Lactate accumulation has been observed in the brain with intracerebral hemorrhage (ICH). However, the outcome of lactate accumulation has not been well characterized. Here, we report that lactate accumulation contributes to angiogenesis and neurogenesis in ICH. In the first set of the experiment, a rat model of ICH was induced by injecting collagenase into the brain. The effects of lactate accumulation on the neurological function, apoptosis, and numbers of newborn endothelial cells and neurons, as well as the proliferation-associated signaling pathway, were evaluated in the rat brain. In the second set, exogenous L-lactate was infused into intact rat brains so that its effects could be further assessed. Following ICH, lactate accumulated around the hematoma; the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were significantly increased compared with the numbers in the Sham group. Moreover, ICH induced translocation of nuclear factor-kappa B (NF-κB) p65 into the nucleus, resulting in a notable upregulation of VEGF and bFGF mRNAs and proteins compared with the levels in the Sham controls. Administration of a lactate dehydrogenase inhibitor dramatically inhibited these effects, decreased the vascular density, and aggravated neurological severity scores and apoptosis after ICH. After exogenous L-lactate infusion, the numbers of PCNA+/vWF+ nuclei and PCNA+/DCX+ cells were strikingly increased compared with the numbers in the Sham controls. In addition, lactate facilitated NF-κB translocation to induce increased transcription of VEGF and bFGF. Co-infusion with an NF-κB inhibitor significantly inhibited these effects. These data suggest that lactate potentiates angiogenesis and neurogenesis by activating the NF-κB signaling pathway following ICH.

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