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- 저자 : Jinqiu Huang (검색결과 2 건)
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Pengfei Liao,Jinqiu Huang,Puguo Tong,Wang Nie,Xin Yan,Yingmei Feng,Hui Peng,Xiaojue Peng,Shao-Bo Li 한국유전학회 2017 Genes & Genomics Vol.39 No.5
Genes encoding inositolphosphorylceramide synthase (IPCS), which play essential roles in sphingolipid biosynthesis, have been known to be widely distributed in plants. However, reports on identification, expression, and function analysis of plant IPCS family genes are very limited. In this study, a total of three OsIPCS genes were identified in the rice database, and their full-length coding regions were amplified by RT-PCR. These genes had 12–13 exons, and they encoded proteins of 313–326 amino acids with molecular mass of 35.8–37.6 kDa and isoelectric point of 10.99–12.61. Importantly, two typical and conserved motifs (D3 and D4) in plants were found in all the three putative OsIPCS proteins, indicating high similarity of OsIPCSs to the other plant IPCS proteins, which were confirmed by phylogenetic analysis. The transcripts of OsIPCS genes appeared in different rice organs including seedling roots, stems and leaves, and young panicles, but each gene showed a unique organ-specific expression pattern. Meanwhile, we found that all the three OsIPCS genes showed responses to stresses like drought, cold and salt, but their expression patterns were also to some extent different under stress treatments. Especially, under cold stress, OsIPCS1 and OsIPCS2 were significantly up-regulated in roots and stems but down-regulated in leaves, while OsIPCS3 was significantly up-regulated in all the measured organs. Actually, putative abiotic-stress regulatory elements were identified in the promoters of these genes. Our data suggest that OsIPCS genes should play important roles during rice growth and adversity adaptation.
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Yumei Li,Rui Zhao,Xiang Li,Chuying Wang,Huiwei Bao,Shudan Wang,Jing Fang,Jinqiu Huang,Ce Wang 한국섬유공학회 2019 Fibers and polymers Vol.20 No.2
The endothelialization and anti-thrombotic abilities of tissue engineered vascular scaffolds are considered to be effective properties for improving the performance small-caliber vascular scaffolds. For this purpose, we designed and developed electrically conductive fibrous scaffolds based on polyaniline coated polyurethane (PANI-PU) electrospun fibersfor vascular tissue engineering applications. The porosity of PANI-PU fibers was 75.27±2.04 %. The obtained PANI-PU fibers were characterized by SEM observations, XPS analysis, water contact angle (WCA) measurement and mechanical property. The PANI functionalization aimed to improve the performance of anticoagulation and endothelialization. The WCA of PAIN-PU decreased to 35 o from 135 o of PU fibers. Blood compatibility and cytocompatibility were compared before and after PANI coating. The adhered platelet cells on PANI-PU was 6.87×105/cm2 and plasma recalcification time was 123 s. Platelet adhesion and plasma recalcification time test showed that the PANI-PU scaffolds had a certain anticoagulant effect. The hemolysis rate of PANI-PU fibers was 0.14 %, which showed that the PANI-PU scaffolds could be used as blood contact materials. The observation of endothelial cell proliferation and morphology in human umbilical vein endothelial cells showed that PANI-PU fibers were more beneficial to cell adhesion, proliferation and extension than that of PU fibers. The results demonstrates the PANI coated electrospun PU fibers have great potential in application as small-diameter vascular grafts and this work shows new insights into conductive scaffolds for vascular tissue engineering.
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