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건강인에서 결핵균 30-kDa항원이 말초혈액 임파구증식에 미치는 영향
최대경,고석신,김화중,조은경,백태현,신철식 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1
T lymphocytes are thought to play a central role in cell mediated immune response. To Study the T lymphocyte proliferative response to 30-kDa antigen purified from Mycobacterium tuberculosis H37Rv, peripheral blood lymphocytes isolated from healthy subjects were stimulated with the 30kDa antigen, crude antigen or PHA. Healthy subjects were divided into three groups so that PPD(+), PPD(±) and PPD(-). The proliferations of lymphocytes were measured by the incroporation of ^3H-thyrnidine. The results were summarized as follows: 1. For the PPD(+) person, at concentration of < 1 ㎍/ml, the proliferative response to 30-kDa antigen resulted in a mild reduction but crude antigen showed relatively rapid reduction. For the PPD(-) person, decreasing the concentration of the both antigens showed rapid reduction of proliferation 2. The lymphocytes proliferations to 30-kDa antigen and crude antigen were significantly increased in PPD(+) group when compared to those in PPD(-) group, but PHA response was no significant difference. 3. The sensitivity and specificity of lymphocyte proliferation assay to 30-kDa antigen and crude antigen when compared with skin test results were 92.3%, 100% and 76.9%, 50%, respectively.
2004년 7월 봇돌바다의 영양염과 Chlorophyll-a의 단기 변동
최용규,조은섭,권기영,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.4
In order to study the temporal variations of nutrients and chlorophyll-a in the Bottol Bada, three field observations were carried out on 20, 23 and 26 July, 2004. The low N:P values exhibit nitrogen deficiency during the periods of observation. This result is not representative of typical summer environment in the southern coast of Korea. The possible mechanisms are as follows: 1) The freshwater inflow was not sufficient for the supply of nitrogen because the total precipitation was 11.9 mm in July, 2004. This amount is no more than 5% in normal precipitation in July. 2) There was an inflow of oceanic water under the subsurface into the Bottol Bada. Even though the oceanic water comprises more nutrients, it produces the stratification between the surface and the subsurface water and seems to prevent the supply of nutrinets to the surface layer. 3) The high chlorophyll-a concentration of 1.2 ㎍/L was shown near the narrow channel between Gae-do and Geumo-do. This seems to be resulted from the inflow of water from Gamak Bay.
생쥐에서 치주조직 형성 과정에 따른 치주인대 세포-특이 PDLs17 단백질 발현 변화
최치원,김은미,이명화,김흥중,박주철 조선대학교 구강생물학연구소 2002 Oral Biology Research (Oral Biol Res) Vol.26 No.2
PDLs17, PDL-specific gone, had previously identified the cDNA for a novel protein from cultured PDL fibroblasts using subtraction hybridization between gingival fibroblasts and PDL fibroblasts. The purpose of this study was to observe the immunohistochemical localization of PDLs17 on the developing periodontium of mice. Developing mice (post-natal mice; P4, P10, P15) were recovered and decapitated, and the heads were fixed overnight at in a freshly prepared solution of 4% paraformaldehyde. Some specimens were decalcified for 2-4 weeks in a solution containing 10% of the disodium salt of ethylenediamine-tetraacetic acid (EDTA). Next, tissues were dehydrated, embedded in paraffin and sectioned serially at 6㎛ in thickness. Polyclonal antiserum raised against PDLs17 peptides. SEAVHETDLHDGC, were made, and immunostained the tooth, periodontium, developing bone and bone marrow of developing mice. The results were as follows. 1. Immunohistochemical analysis showed that PDLs17 is Preferentially expressed in the developing tooth follicle, but not in tooth pulp and overlying gingival mucosa of 4-day-old developing mouse. PDLs17 also expressed in preosteoblast-likec ells in the developing alveolar bone of 4-day-old mouse. 2. PDLs17 expressions were observed in developing tooth follicle, tooth pulp and alvolar bone of 10-day-old developing mouse. 3. PDLs17 protein were observed in PDL cells, tooth pulp and surrounding alveolar bone of 15-day-old tooth-eloping mouse. However, there were no PDLs17 expression in ameloblasts and odontoblasts. These results suggested that PDLs17 might expressed at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL, differentiatiation from tooth follicle. However, more research needs to be performed to gain a better understanding of the exact function of PDLs17 during the differentiation of tooth pulp cells and PDL cells.
최은화,서창호,임종태,김석우 公州大學校 工學硏究院 生産技術硏究所 2003 論文集 Vol.11 No.-
Biometric encryption refers to a process of secure key management. Biometric encryption does not directly provide a mechanism for the encryption/decryption of data, but rather provides a replacement to typical passcode key-protection protocols. Although the process of biometric encryption can be applied to any biometric image, the initial implementation was achieved using fingerprint images.
2004년 7월 봇돌바다의 썰물, 전류 및 밀물시 수온과 염분 분포
최용규,조은섭,이용화,이영식 한국환경과학회 2005 한국환경과학회지 Vol.14 No.2
Based on the observation on 20, 23 and 26 July 2004, the distributions of temperature, salinity and stratification was investigated in relation to ebb, turn of tide and flood. The results are as follows: 1) The high temperature and low saline water with 23.5-24.0℃ and 32.4~33.0psu existed at Naro Island. 2) The cold surface water below 21.00 and 33.0~33.4psu appeared in the area near Gae Island and Geumo Island. 3) The cold and saline water, below 24.0℃ at the surface and 17.0℃ near the bottom, 32.8~33.8psu at the surface and 33.8~34.0psu near the bottom, existed in Sori Island. These waters were more saline compared to the South Sea Coastal Water with about 31.8psu. This suggests that the oceanic saline water intruded into the Bottol Bada through the area near Sori Island. The stratification appeared during all the observation periods due to a high solar radiation of 22MJ/m², and a weak wind speed of 2.9m/s on the average while the mean speed of wind in July is around 3.9 m/s. It qualitatively suggested that the stratification was maintained during the observation periods because of a high solar radiation, a weak wind speed and intrusion of saline oceanic water.
흰쥐 선조체에서 6-OHDA-유도 도파민 고갈 및 SH-SY5Y 세포주에서 6-OHDA-유도 산화적 스트레스에 대한 I-Deprenyl의 신경 보호효과
김은미,최신규,이경림,김화정 이화여자대학교 약학연구소 2005 藥學硏究論文集 Vol.- No.15
A neurotoxin, 6-hydroxydopamine (6-OHDA) has long been used to form a Parkinson's disease (PD) model by inducing the lesion in catecholaminergic pathways, particularly the nigrostriatal dopamine (DA) pathway. Whereas I-deprenyl, a selective inhibitor of monoamine exidase (MAO) type B, is now widely used in the treatment of PD, the precise action mechanism of the drug remains elusive. In this studym, we investigated whether I-deprenyl shows protective effect against the DA depletion induced by 6-OHDA in rat brain, and against 6-OHDA-induced neurotoxicity and oxidative stress in catecholaminergic neuroblastoma SH-SY5Y cells that are known to lack MAO-B activity. Pretreatment fo I-deprenyl significantly enhanced the striatal DA, 3,4-dihydroxyphenylacetic acid, homovanilic acid, and 3-methoxytyramine levels compared to the untreated 6-OHDA-lesioned rat, indicating that I-deprenyl pretreatment prevents 6-OHDA-induced depletion of not only striatal dopamine but also its metabolites. Treatment of 6-OHDA for 24 hrs decreased the cell viability and increase the generation of ROS in dose-dependent manners.We further investigated whether caspase activity is involved in the action of I-deprenyl. Treatment of I-deprenyl (0.1~100μM) did not produce any changes in 6-OHDA-induced cleavage of poly (ACP-ridose) polymerase in SH-SY5Y cells. Our result suggest that the neuroprotective effect of I-deprenyl against 6-OHDA is due to its incresed scavenger activity, but independent of inhibition of MAO-B or caspase-3activation.